Category Archives: Food Microbiology Research

Research – Multi-country outbreak of Salmonella Mbandaka ST413, possibly linked to consumption of chicken meat in the EU/EEA, Israel and the UK

EFSA

A cross-border outbreak of Salmonella Mbandaka ST413 has been ongoing in the EU/EEA, Israel, and the UK since September 2021. By 8 November 2022, 196 cases had been reported in Czechia (n=5), Estonia (n=3), Finland (n=89), France (n=10), Germany (n=2), Ireland (n=1), the Netherlands (n=1), the United Kingdom (n=81), and Israel (n=4), according to the European case definition. Nineteen cases were hospitalised and five cases had septicaemia. One case in the UK died.

Based on case interviews from Finland and the UK, ready-to-eat (RTE) chicken products and/or fresh chicken meat are the likely vehicles of infection. Fifteen cases in Finland reported consumption of six RTE products from three brands. All 15 cases had consumed at least one RTE chicken product. Two products were marketed under the same brand name, which is adopted by the Estonian Company A and the Finnish Company B. Based on additional information, such as purchase data, and consultation of the grocery chains selling the products and of the Finnish Company B, the food authority in Finland linked the products to the Estonian Company A. However, this link could not be verified by the identification of the batches nor by microbiological evidence. The Estonian Company A received processed chicken meat from different suppliers, but there is no further information in RASFF. Therefore, the role of the Estonian Company A as a source of infection could not be established.

Epidemiological data and microbiological evidence from whole genome sequencing of human isolates indicate there are several active sources through different food distribution chains, with a likely common source higher up in the chicken supply chain. New cases are likely to occur in the EU/EEA until the source has been identified and controlled.

Further investigations are needed by public health and food safety authorities to identify the source(s)/origin of contamination.

Click to access roa-7707.pdf

Research – Treatment of Fresh Meat, Fish and Products Thereof with Cold Atmospheric Plasma to Inactivate Microbial Pathogens and Extend Shelf Life

MDPI

Abstract

Assuring the safety of muscle foods and seafood is based on prerequisites and specific measures targeted against defined hazards. This concept is augmented by ‘interventions’, which are chemical or physical treatments, not genuinely part of the production process, but rather implemented in the framework of a safety assurance system.
The present paper focuses on ‘Cold Atmospheric pressure Plasma’ (CAP) as an emerging non-thermal intervention for microbial decontamination. Over the past decade, a vast number of studies have explored the antimicrobial potential of different CAP systems against a plethora of different foodborne microorganisms.
This contribution aims at providing a comprehensive reference and appraisal of the latest literature in the area, with a specific focus on the use of CAP for the treatment of fresh meat, fish and associated products to inactivate microbial pathogens and extend shelf life. Aspects such as changes to organoleptic and nutritional value alongside other matrix effects are considered, so as to provide the reader with a clear insight into the advantages and disadvantages of CAP-based decontamination strategies.

Research – Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters

MDPI

french oysters

Abstract

Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practices can represent one of the main strategies to reduce the incidence of viral diseases related to shellfish. This study aimed to investigate long-term relays for the reduction of NoV levels in live oysters, during the high-risk cold months, by transferring the oysters from a more contaminated site to two sites with lower NoV levels. The efficacy of relaying was evaluated by analyzing oyster samples collected at days 0 (T0) and 30 (T30) for NoV levels using a real-time quantitative PCR (RT-qPCR). The NoV level at the relay sites was consistently lower than at the harvest site. The relay process for 30 days in seawater with a lower NoV level resulted in a decrease in the NoV load compared to day 0 with significant reductions depending on the site and genogroup of NoV considered. These results suggest that long-term relaying of oysters to reduce NoV levels is promising and could help growers to improve oyster safety; however, further investigations are needed.

Research – Occurrence of Aflatoxins and Ochratoxin a during Merkén Pepper Powder Production in Chile

MDPI

Abstract

Berry fruits of Capsicum annuum L. cv. “Cacho de Cabra” are used for the manufacture of a traditional pepper powder known as Merkén. In the present study, aflatoxins (AFs) and ochratoxin A (OTA) contamination in berry fruits of C. annuum was determined at harvest, drying, and smoking stages of Merkén production, in cumin and coriander seeds used as Merkén ingredients, and in the final packaged Merkén produced by local farmers. Additionally, Merkén samples from local markets in the region of La Araucanía (Chile) were also evaluated. Chromatographic analysis was based on a qualitative method. AFs and OTA were not detected on pepper pods and seeds. There was no detection of AFs and OTA on cultured Aspergillus and Penicillium strains isolated from pepper pods, cumin and coriander seeds and Merkén. The lack of AFs/OTA-producers among the isolated fungal species can explain and support the absence of contamination in pepper pods. In contrast, the AFB1 was detected in 75% of Merkén obtained from farmers and 46% of Merkén samples purchased from local markets; while OTA was detected in 100% of Merkén samples obtained from farmers and local markets. In the Merkén production chain, the harvest and post-harvest are key stages for fungal growth while the commercialization stage is highly susceptible to AFs and OTA contamination.

Research – One Health Campylobacter Risk Model Could Improve Outbreak Surveillance

Food Safety.Com

Researchers have built a new risk model for possible human Campylobacter outbreaks that leverages data on weather patterns and the presence of Campylobacter on broiler farms. The developers of the model hope to disseminate the information it produces through a website that can generate warnings when appropriate.

The model follows a “One Health” approach, in that data from the animal, human, and environmental sectors were combined to predict human gastrointestinal illness (GI) outbreaks.

Read more at the link above

Research – From Aflatoxin to Zearalenone: Mycotoxins You Should Know – Deoxynivalenol (DON)

Agfax

Mycotoxins are substances produced by fungi that infect grain crops like maize and small grains and cause ear and kernel rots. Exposure to mycotoxins can lead to chronic or acute toxicity in humans and animals. In addition, mycotoxins can lead to market losses, discounts, rejection of grain lots at elevators, and a reduction in livestock efficiency and productivity.

The most economically important mycotoxins include aflatoxins (AF), deoxynivalenol (DON, also known as vomitoxin), fumonisins (FUM), zearalenone (ZEA), ochratoxin A (OTA), T2, HT-2, ergot alkaloids, and patulin (PAT). The fungal species that produce mycotoxins have worldwide distribution; therefore, mycotoxin contamination occurs everywhere grain crops are grown. Accordingly, mycotoxins have been detected in feed, silage, food, and beverages derived from cereal grains and animal products exposed to contaminated feed.

Research – Warning after scientists find faeces and dangerous bacteria at self-service check-outs

News Yahoo

Health chiefs are warning festive shoppers to wash their hands regularly after a study found both faeces and vomit-inducing bugs – on self-service check-out screens.

The investigation, where scientists analysed swabs from several everyday objects, revealed there were thousands of bacteria on the ubiquitous payment machines.

E.coli, which causes vomiting, was present on nearly all the surfaces, while poo and microbes that lead to urinary tract infections (UTIs) were found on self-service screens.

A bug commonly found in the vagina, mouth, throat and gut called Candida albicans, which can cause yeast infections, was also discovered on an escalator handrail.

And shoppers may also be at risk if they hot desk at work, as intestinal microbes that can cause a range of infections, including UTIs, were found on computer keyboards.

Italy hit by new Listeria outbreak with one dead

Food Safety News

Italian authorities are investigating a Listeria outbreak that has sickened 22 people and killed one.

Mortadella sold by Veroni is being looked at as a potential source but an official link has not been confirmed by authorities.

In early November, a number of recalls were published for Veroni mortadella due to potential Listeria contamination.

A product recalled by Veroni in November

The Ministry of Health (Ministero della Salute) issued a warning covering a few batches of the Veroni Supergiant Mortadella packed in small portions due to the presence of Listeria monocytogenes.

Veroni said it immediately and voluntarily alerted the market, recalling all batches of Supergiant Mortadella with and without pistachios in portions that had expiry dates before Dec. 27, 2022.

USA – Outbreak Investigation of E. coli O121:H19 Frozen Falafel (October 2022)

FDA

The FDA, along with CDC and state and local partners, investigated a multistate outbreak of E. coli O121:H19 infections linked to Earth Grown frozen falafel.

As of December 1, 2022, CDC announced that the outbreak is over. There have been a total of 24 illnesses in six states. The last illness onset was October 24, 2022.

On October 7, 2022, the Michigan Department of Agriculture and Rural Development collected Earth Grown frozen falafel for testing from a sick person’s home. Whole Genome Sequencing done at the Michigan Department of Health & Human Services showed that the E. coli strain in the falafel sample was closely related to the strain causing illness in this outbreak.

On October 6, 2022, the manufacturer of Earth Grown frozen falafel, Cuisine Innovations, initiated a voluntary recall. Recalled products include Earth Grown vegan traditional falafel and garlic & herb falafel distributed and sold exclusively by ALDI. Additional information on recalled products is available in the firm’s recall notice.

Recommendation

These items are no longer available for sale but were sold frozen and have a long shelf-life of 18 months, so consumers should check their freezers for any recalled Earth Grown vegan traditional falafel and garlic & herb falafel that was sold by ALDI. If you have recalled frozen falafel in your freezer, do not eat or serve them.


Product Images

Outbreak Investigation E.coli Frozen Falafel October 2022 - Sample Product Images

Map of U.S. Distribution

Outbreak Ecoli Falafel Distribution Map

Case Count Map Provided by CDC

Outbreak Investigation of E. coli O121: Frozen Falafel (October 2022) - CDC Case Count Map as of December 1, 2022

Case Counts

Total Illnesses: 24
Hospitalizations: 5
Deaths: 1
Last illness onset: October 24, 2022
States with Cases: FL (2), IA (2), KS (1), MI (13), OH (1), WI (5)
Product Distribution: Nationwide

RASFF Alert – STEC E.coli – Bovine Meat – Salami –

RASFF

STEC (stx+;eae+) in bovine meat from Belgium in Bulgaria, France, Greece and the Netherlands

RASFF

E.coli (STEC) is found in salami from Italy in Denmark

RASFF

Verotoxin producing Escherichia coli in salami with wild boar from Italy in Austria, Croatia, Denmark, France, Germany, Hungary, Ireland, Italy, Sweden and Switzerland