Category Archives: E.coli

Research – Risk of Foodborne Illness from Pet Food: Assessing Pet Owners’ Knowledge, Behavior, and Risk Perception

JFP

Pet food has been identified as a source of pathogenic bacteria, including Salmonella and Escherichia coli. A recent outbreak linked to Salmonella -contaminated pet treats infected over 150 people in the United States. The mechanism by which contaminated pet food leads to human illness has not been explicated. Pet owners’ food safety knowledge and their pet food handling practices have not been reported. This study evaluated pet owners’ food safety knowledge and pet-food handling practices through an online consumer survey. The survey consists of 62 questions and assesses (1) owners’ food safety knowledge and pet-food handling practices; (2) owners’ interaction with pets; (3) owners’ risk perception related to their own health, their children’s health, and their pets’ health. The survey was pilot-tested among 59 pet owners before distribution to a national consumer panel, managed by Qualtrics XM. All participants (n=1,040) were dog and/or cat owners in the United States. Almost all pet owners interacted with their pets (93%) and most cuddled, allowed their pets to lick them, and slept with their pets. Less than one-third of pet owners washed their hands with soap after interacting with their pets. Over half (58%) the owners reported washing their hands after feeding their pets. Most pet owners fed their pets dry pet food and dry pet treats. Some fed their pets raw meat or raw animal product (RAP) diets because they believed these diets to be beneficial to their pet’s overall health. Many owners (78%) were unaware of pet food recalls or outbreaks associated with foodborne pathogens. Less than 25% considered dry pet foods and treats as a potential source of foodborne pathogens. The findings of this study indicated the need for consumer education about pet food handling. The data collected can assist in developing more accurate risk assessment models and consumer education related to pet food handling.

RASFF Alert – E.coli – Live Clams

RASFF-Logo

RASFF – too high count of Escherichia coli (790 MPN/100g) in live clams (Ruditapes philippinarum) from Italy in Italy

Research -Sneaky Salmonella finds a backdoor into plants

News Wise

Newswise — As the world wrestles with the coronavirus (COVID-19) pandemic, which arose after the virus jumped from an animal species to the human species, University of Delaware researchers are learning about new ways other pathogens are jumping from plants to people.

Opportunistic bacteria — salmonella, listeria and E.coli, for example — often piggyback on raw vegetables, poultry, beef and other foods to gain entry into a human host, causing millions of foodborne illnesses each year.

But University of Delaware researchers Harsh Bais and Kali Kniel and their collaborators now have found that wild strains of salmonella can circumvent a plant’s immune defense system, getting into the leaves of lettuce by opening up the plant’s tiny breathing pores called stomates.

The plant shows no symptoms of this invasion and once inside the plant, the pathogens cannot just be washed off.

Stomates are little kidney-shaped openings on leaves that open and close naturally and are regulated by circadian rhythm. They open to allow the plant to cool off and breathe. They close when they detect threats from drought or plant bacterial pathogens.

Research – From Jekyll to Hyde: New study pinpoints mutation that makes E. coli deadlier

Science Daily

As far as humans are concerned, bacteria can be classified as either harmful, pathogenic bacteria and harmless or beneficial non-pathogenic bacteria. To develop better treatments for diseases caused by pathogenic bacteria, we need to have a good grasp on the mechanisms that cause some bacteria to be virulent. Scientists have identified genes that cause virulence, or capability to cause disease, but they do not fully know how bacteria evolve to become pathogenic.

To find out, Professor Chikara Kaito and his team of scientists from Okayama University, Japan, used a process called experimental evolution to identify molecular mechanisms that cells develop to gain useful traits, and published their findings in PLoS Pathogens. “We’re excited by this research because no one has ever looked at virulence evolution of bacteria in an animal; studies before us looked at the evolution in cells,” said Prof Kaito.

The scientists decided to start with a non-pathogenic Escherichia coli (or E. coli for short) and repeatedly mutate it and use it to infect silkworms, an insect that is often used as a model for infectious diseases, and then test whether it will cause death in silkworms.

South Korea – E. coli infections tied to kindergarten south of Seoul rise to 58

YNA

SEOUL, June 29 (Yonhap) — The number of people infected with a strain of E. coli bacteria in connection to a kindergarten just south of Seoul reached 58, one more from the previous day, health authorities said Monday.

Health authorities have widened a probe into the E. coli outbreak in Ansan, about 50 kilometers south of the capital, since a kindergarten student first showed symptoms of illness on June 12.

As of 6 p.m. Sunday, 114 students and their family members showed suspected symptoms, according to the Korea Centers for Disease Control and Prevention (KCDC).

Of 21 hospitalized patients, 16 people, including 14 students, showed symptoms of hemolytic uremic syndrome (HUS), a type of E. coli that can cause kidney failure. Of them, four are undergoing dialytic therapy.

Research – Salmonella, Listeria and E. coli: opportunistic bacteria

Oulah

Researchers at the University of Delaware are examining how certain bacteria manage to bypass plant immune defenses.

As the world battles against the coronavirus pandemic (COVID-19), which emerged after the virus moved from animal to human, researchers at the University of Delaware are learning new ways to other pathogens jump from plants to humans

Opportunistic bacteria,  Salmonella ,  Listeria  and  E. coli , for example – often attach themselves to raw vegetables, poultry, beef and other foods to enter a human host, causing millions of illnesses each year food.

But researchers from the University of Delaware,  Harsh Bais  and  Kali Kniel  and their collaborators have now discovered that wild strains of  Salmonella  can bypass a plant’s immune system, penetrating lettuce leaves by opening tiny pores. of the plant called stomata.
The plant has no symptoms of this invasion and once inside the plant, the pathogens cannot simply be washed out.

Research – Antibacterial activities of Camellia sinensis plant extracts against uropathogenic E. coli in vitro and in vivo

AJ

This study evaluated the in vitro and in vivo antibacterial activities of methanol and ethyl acetate extracts of Camellia sinensis (Green tea) against Escherichia coli implicated in urinary tract infections. Antibacterial activities of these plant extracts were tested against 10 multi-drug resistant E. coli isolates obtained from clinical specimens of UTI patients. Forty-five (45) Wister albino rats were used for this study and exactly 0.1 ml of standardized (0.5 McFarland’s) E. coli suspension was each inoculated into the 45 rats through intra-urethral route and observed after 48 h. This was followed by oral administration of different concentrations of methanol and ethyl acetate extracts of C. sinensis, and ciprofloxacin antibiotic for 14 days. Phytochemical screening of extracts showed the presence of bioactive components. Results revealed that methanol extract was better than ethyl acetate extract of C. sinensis in the treatment of UTI caused by E. coli. Body weight, white blood cell count, and erythrocyte sedimentation rate level returned to normal values after treatment with methanol extract of C. sinensis at 200 mg/kg body weight when compared to ethyl acetate extract of C. sinensis. This study has shown that C. sinensis possess bioactive ingredients with antimicrobial activities. Information from this study adds to the current information on the potential health benefits of green tea. Thus, further studies on other plant products should be explored so as to understand their potential health benefits and as alternative therapeutics in the treatment of bacterial infections.

Research – A bacterium that causes food-borne illness grows flagella under stressful conditions

Massive Science CDC E.coli

Micro-organisms, especially bacteria, play essential roles in our bodies, especially in our guts. Some bacteria are beneficial, and some like E.coli are harmful. Another Escherichia strain (in the same genus as E. coli) named Escherichia albertii is also pathogenic to humans, causing diarrhea and food-borne illnesses.  E. albertii was identified for the first time during an illness outbreak in Bangladesh.

Pathogenic bacteria like E. albertii are very motile, meaning they move around a lot. They are able to do this using hair-like structures called flagellaE. albertii was originally described as non-hairy bacterium and thus far has been considered to be a non-motile pathogenic micro-organism.

 

USA – Raw Beef Ravioli recalled over E. coli fears

Food Poison Journal

The U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) is issuing a public health alert because approximately 70 pounds of raw beef ravioli products, produced by P&S Ravioli Company, a Philadelphia, Pa. establishment, may be contaminated with E. coli O157:H7. A recall was not requested because the affected product is no longer available for purchase.

However, FSIS is concerned that some product may be in consumers’ freezers. Consumers who have purchased these products should not consume them. These products should be thrown away or returned to the place of purchase.

South Korea – Food poisoning sickens about 100 kindergarten pupils in Ansan

YNA

ANSAN, South Korea, June 25 (Yonhap) — About 100 pupils at a kindergarten in Ansan, just south of Seoul, have complained of food poisoning symptoms over the past week, with a fifth of them hospitalized for hamburger disease and other disorders, municipal health officials said Thursday.

According to the officials in Ansan, 50 kilometers south of the capital, 99 out of 184 children attending the unidentified kindergarten showed symptoms of food poisoning from June 16 to Monday, and 22 of them were sent to hospitals.

Some of them are reportedly showing signs of hemolytic uremic syndrome (HUS), also known as hamburger disease, which could cause severe kidney failure, they noted, adding doctors raised the possibility of hamburger disease among some ailing students.

The number of patients has been increasing since four children complained of stomachaches on June 16. By June 17, 10 pupils exhibited symptoms of stomachache and diarrhea.

Authorities have found hemorrhaging E. coli bacteria in samples taken from about 30 pupils. HUS is one of the complications caused by E. coli bacteria.