Category Archives: STEC

UK- Scotland – Guidance for Scottish local authority officers on the production of cheese from unpasteurised milk

HPS

The Scottish Food Enforcement Liaison Committee (SFELC) has endorsed guidance produced by their Joint Specialist Cheese and Risky Foods Short-Life Working Group on the production of cheese from unpasteurised milk.

The guidance aims to control the microbiological risks in the production of artisan cheeses made from unpasteurised milk, specifically Shiga toxin-producing Escherichia coli(STEC). The guidance applies to all establishments producing cheese made from unpasteurised milk from cows, goats, sheep and buffalo.

Cheese made from unpasteurised milk has been linked to outbreaks of human illness and in particular, illness caused by STEC organisms including E. coli O157. The guidance has been developed in relation to the control of STEC and is intended for use during the inspection and enforcement of food safety controls applied by cheesemakers producing cheese made from unpasteurised milk.

The guidance reflects current scientific knowledge and understanding in relation to STEC and the production of cheese from unpasteurised milk. As scientific information and evidence on STEC evolves, the guidance will be reviewed. In addition, the guidance will complete a pilot implementation phase before a scheduled review.

The guidance is available to view at: https://www.foodstandards.gov.scot/downloads/SFELC_Document.pdf.

Details of a recent report on pathogen risks in unpasteurised cheese in Scotland can be viewed on the HPS weekly report, current note 52/5006: http://www.hps.scot.nhs.uk/ewr/redirect.aspx?id=78563.

RASFF Alert- STEC E.coli – Raw Sausage

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RASFF -shigatoxin-producing Escherichia coli in raw sausage from Austria in Austria

Research – Detection, Isolation, and Characterization of Shiga Toxin–Producing Escherichia coli in Flour

Journal of Food Protection

Wheat flour has recently been described as a novel vehicle for transmission of Shiga toxin–producing Escherichia coli (STEC). Very recently, an outbreak of STEC O121 and STEC O26 infections was linked to flour in the United States. The aim of the present study was to generate baseline data for the occurrence of STEC in flour samples from different retailers in Switzerland. In total, 70 flour samples were analyzed. After enrichment, the samples were screened for stx1 and stx2 by the Assurance GDS MPX ID assay. STEC strains were isolated and serotyped by the E. coli SeroGenoTyping AS-1 kit. The determination of stx subtypes was performed with conventional PCR amplification. Screening for eae, aggR, elt, and estIa/Ib was performed by real-time PCR. Nine (12.9%) of the flour samples tested positive for stx by PCR. STEC was recovered from eight (88.9%) of the positive samples. Two isolates were STEC O11:H48 harboring stx1c/stx1d, two were O146:H28 containing stx2b, one was O103:H2 containing stx1a and eae, and three were O nontypeable: Ont:H12 (stx2a), Ont:H14 (stx2a/stx2g), and Ont:H31 (stx1c/stx1d). STEC O103 belongs to the “top five” serogroups of human pathogenic STEC in the European Union, and STEC O146 is frequently isolated from diseased humans in Switzerland. Our results show that flour may be contaminated with a variety of STEC serogroups. Consumption of raw or undercooked flour may constitute a risk for STEC infection.

RASFF Alerts – STEC E.coli -Chilled Boneless Beef – Farmstead Cheese

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RASFF-shigatoxin-producing Escherichia coli (in 1 out of 5 samples /25g) in chilled boneless beef from Argentina in Germany

RASFF-shigatoxin-producing Escherichia coli (presence /25g) in farmstead cheese from France in Germany

RASFF Alert – STEC E.coli – Chilled Lamb Meat

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RASFF-enteropathogenic Escherichia coli (stx2- /25g) in chilled lamb meat from the United Kingdom in the Netherlands

RASFF Alert – STEC E.coli – O26 – Raw Goats Milk Cheese

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RASFF -enteropathogenic Escherichia coli (026H11 eae+, stx-) in raw goat’s milk cheese from France in France

Research -Microbiological Testing Program for E. coli O157:H7 and non-O157 Shiga Toxin-Producing E. coli: Individual Positive Results for Raw Ground Beef (RGB) and RGB Components

USDA

Table 1. Raw Ground Beef Products (RGB) Analyzed for E. coli O157:H7, Current Calendar Year

Sample Source1 Collection Date Where Collected Product Status Positives this Year Samples Analyzed this Year Total Positives2 Total Samples Analyzed2
Federal RGB Verification, Beef Oct 15, 2018 MN Held 4 9,541 540 238,301
Federal RGB Verification, Beef Oct 9, 2018 NC Held 3 9,297 539 238,057
Federal RGB Verification, Beef Mar 26, 2018 OR Held 2 3,085 538 231,845
Federal RGB Verification, Beef Feb 8, 2018 CA Held 1 1,704 537 230,464

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Microbiological Testing Program for E. coli O157:H7 and non-O157 Shiga Toxin-Producing E. coli: Individual Positive Results for Raw Ground Beef (RGB) and RGB Components

View by Year:  
2018 Positive Results
2017 Positive Results
2016 Positive Results
2015 Positive Results
2014 Positive Results
2013 Positive Results
2012 Positive Results
2011 Positive Results
2010 Positive Results
2009 Positive Results
2008 Positive Results
2007 Positive Results
2006 Positive Results
2005 Positive Results
2004 Positive Results
2003 Positive Results
2002 Positive Results
2001 Positive Results

The table below includes all positive results as of November 4, 2018.

Table 1. Raw Ground Beef Products (RGB) Analyzed for E. coli O157:H7, Current Calendar Year 

Sample Source1 Collection Date Where Collected Product Status Positives this Year Samples Analyzed this Year Total Positives2 Total Samples Analyzed2
Federal RGB Verification, Beef Oct 15, 2018 MN Held 4 9,541 540 238,301
Federal RGB Verification, Beef Oct 9, 2018 NC Held 3 9,297 539 238,057
Federal RGB Verification, Beef Mar 26, 2018 OR Held 2 3,085 538 231,845
Federal RGB Verification, Beef Feb 8, 2018 CA Held 1 1,704 537 230,464

1Sample Sources may include these types of establishments and samples:

  • Federal (verification; follow-up)
  • Retail (verification; follow-up)
  • State (verification; follow-up)
  • Import (verification; follow-up). For Import samples, the column “Where Collected” is defined as Country of Origin. <!–
  • Source may also refer to the type of product (beef, veal, or mixed), as listed on the product label.
  • –>

2Totals: “Total Positives” and “Total Samples Analyzed” are the totals since FSIS began its testing program to detect E. coli O157:H7 in raw ground beef on October 17, 1994.


The table below includes all positive results as of November 4, 2018.

Table 2. Raw Ground Beef Components (RGBC) Analyzed for Target STECs, Current Calendar Year3

Sample Source4 Collection Date Target STECs Where Collected Product Status Posi-
tives this Year
Samples Analyzed this Year5 Total Posi-
tives
Total Samples Analyzed6
Trim Verification, Beef Oct 18, 2018 O111 PA Held 19 6,594 415 59,239
Trim Verification, Beef Oct 9, 2018 O157:H7 SD Held 18 6,262 414 58,907
Trim Verification, Beef Sep 5, 2018 O157:H7 PA Held 17 5,641 413 58,286
Trim Verification, Beef Aug 14, 2018 O103 PA Held 16 5,018 412 57,663
Trim Verification, Beef Jun 6, 2018 O157:H7 MO Held 15 3,583 411 56,228
Trim Verification, Veal Jun 6, 2018 O26 PA Held 14 3,428 410 56,073
Follow-up to RGBC Positive, Beef May 30, 2018 O103 PA Held 13 3,266 409 55,911
Follow-up to RGBC Positive, Beef May 29, 2018 O103 PA Held 12 3,266 408 55,911
Trim Verification, Beef May 21, 2018 O157:H7 TX Held 11 3,147 407 55,792
Trim Verification, Beef May 16, 2018 O103 PA Held 10 2,968 406 55,613
Trim Verification, Beef May 15, 2018 O103 NY Held 9 2,968 405 55,613
Trim Verification, Beef May 9, 2018 O103 PA Held 8 2,968 404 55,613
Other RGBC Verification May 9, 2018 O157:H7 NE Held 7 2,968 403 55,613
Trim Verification, Beef Mar  19, 2018 O157:H7 NM Held 6 1,814 402 54,455
Trim Verification, Beef Mar 1, 2018 O121 ID Held 5 1,507 401 54,148
Trim Verification, Veal Feb 27, 2018 O103 WA Held 4 1,347 400 53,988
Follow-up to RGBC Positive, Beef Jan 27, 2018 O45 WI Held 3 583 400 53,225
Other RGBC Verification Jan 4, 2018 O157:H7 SD Held 2 107 399 52,749
Trim Verification, Beef Dec 28, 2017 O157:H7 WI Held 1 107 398 52,749