Category Archives: STEC

RASFF Alerts – STEC E.coli – Beef

kswfoodworld food safety poisoning

RASFF -shigatoxin-producing Escherichia coli (presence /25g) in frozen beef from Brazil in Italy

RASFF -shigatoxin-producing Escherichia coli (stx1-,stx2+ /25g) in frozen boneless beef from Brazil in Italy

RASFF-shigatoxin-producing Escherichia coli (stx1+, stx2+, eae- /25g) in chilled boneless beef from Uruguay in the Netherlands

RASFF Alert – STEC E.coli – Chilled Beef

kswfoodworld food safety poisoning

RASFF-shigatoxin-producing Escherichia coli (stx2+ /25g) in chilled beef from Uruguay in the Netherlands

RASFF Alert – STEC E.coli – Chilled Beef

kswfoodworld food safety poisoning

RASFF-shigatoxin-producing Escherichia coli (stx1+ stx2+ eae+ /25g) in chilled beef from Uruguay in the Netherlands

UK -Analysis shows source of E. coli outbreak was leafy greens – O157 – 2016

Food Safety News 

CDC E.coli

Image CDC Enter a caption

Increased use of ingredient-based analyses is being promoted by researchers after the method was used to identify the source of infection in an E. coli outbreak. 

Public Health England was alerted to an outbreak of Shiga toxin-producing E. coli (STEC) O157 phage type (PT) 34 in July 2016 involving 56 cases in England and Wales. The source of infection was baby-mixed leaf salad, which was an ingredient in multiple dishes.

“A traditional analysis may have resulted in multiple menu items being associated with illness, thereby failing to identify the true source of infection,” said researchers.

 

Ireland -12 cases of E.Coli now confirmed at Dunmore creche

Connach Tribune

12 cases of E.Coli have now been confirmed following an outbreak last week at Dun Beag creche in Dunmore.
The HSE’s multidisciplinary Outbreak Control Team says four cases have involved hospitalisation.
E.Coli VTEC can spread in a number of ways including contact with infected animals, contaminated soil, water or certain foods, and can be spread among toddlers who are not toilet trained.
The majority of cases of VTEC get better with no treatment and without hospitalisation.
However the most serious complication is Haemolytic Uraemic Syndrome or HUS which occurs in up to 10% of VTEC cases.
This can lead to anaemia and kidney failure and requires intensive medical treatment.
The number of cases of VTEC notified in Ireland each year is increasing with 927 cases confirmed in 2017.

RASFF Alert -STEC E.coli – Raw Milk Cheese

kswfoodworld food safety poisoning

RASFF-shigatoxin-producing Escherichia coli (O26:H11 stx+, eae+ /25g) in raw milk cheese from France in France

Research – A Novel Selective Medium for Simultaneous Enrichment of Shiga Toxin–Producing Escherichia coli and Salmonella in Ground Beef

Journal of Food Protection 

 

Microbiological analysis of ground beef for contamination by both Salmonella and Shiga toxin–producing Escherichia coli (STEC) is performed by the U.S. Department of Agriculture, Food Safety Inspection Service (FSIS), as part of its Performance Standards Verification Testing program. FSIS has established a zero tolerance for STEC serotype O157:H7 and serogroups O26, O45, O103, O111, O121, and O145 because they are regarded as adulterants. The detection and isolation of these specific serogroups presents a technical challenge necessitating time-consuming and costly laboratory procedures that often exceed the technical capabilities of many small internal and reference laboratories. We describe here a method using a novel STEC and Salmonella selective (SSS) broth that allows for simultaneous selective enrichment of STEC and Salmonella sp., providing isolation and detection from the same broth. The method only involves direct plating from beef enrichments to detect suspect isolates that can be easily confirmed by using immunoassays or PCR, rendering the isolation simpler and less costly than the current described methods. In a side-by-side comparison with modified tryptic soy broth (mTSB), the use of SSS broth resulted in primarily isolating STEC and Salmonella sp., while substantially suppressing the growth of other gram-negative Enterobacteriacae by 90%. Significantly more (χ2 < 3.84) samples containing E. coli O157:H7 and STEC O26, O111, O121, and O145 and a nondifferent (χ2 > 3.84) number of samples containing STEC O103 and O45 were identified when enriching in SSS broth. Coenrichment using six different Salmonella serovars showed numerically greater but not significant (χ2 < 3.84) positive samples by using SSS broth compared with mTSB for a majority of serotypes.