Category Archives: STEC

RASFF Alert – STEC E.coli – Chilled Spelt Dough

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RASFF – shigatoxin-producing Escherichia coli (stx1-, stx2+ /25g) in chilled spelt dough from Austria in Germany

USA- Romaine in the cross-hairs – FDA to test lettuce for pathogens

Food Poison Journal

The FDA is conducting a small, focused assignment to collect samples of raw agricultural commodity (RAC) romaine lettuce to test for Salmonella spp. and pathogenic Escherichia coli (also known as Shiga Toxin-producing E. coli or STEC), microbial hazards repeatedly linked to foodborne illnesses associated with romaine lettuce consumption. The assignment begins this month (November 2019) and is expected to last one year.

Research -First report of Escherichia coli O157:H7 in ready-to-eat sushi.

Europe PMC

AIMS:The aim of this study was to evaluate the microbiological quality of commercially prepared ready-to-eat (RTE) sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella ssp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form biofilms on stainless steel. METHODS AND RESULTS:There were four sampling events in seven establishments, totalling 28 pools of sushi samples. Mean AMB counts ranged between 5·2 and 7·7 log CFU per gram. The enumeration of TC varied between 2·1 and 2·7 log MPN per gram. Salmonella ssp. were not detected, and one sample was positive for E. coli and was identified as E. coli O157:H7. To the best of our knowledge, this is the first report of E. coli O157:H7 in sushi samples in the world literature. This isolate presented virulence genes stx1, stx2, eae and hlyA. It was also susceptible to 14 antimicrobials tested and had the ability to form biofilms on stainless steel. CONCLUSIONS:There is a need to improve the good hygiene practices adopted in establishments selling sushi in the city of Pelotas, Brazil. In addition, the isolated E. coli O157:H7 carries a range of important virulence genes being a potential risk to consumer health, as sushi is a RTE food. This isolate also presents biofilm formation ability, therefore, may trigger a constant source of contamination in the production line of this food. SIGNIFICANCE AND IMPACT OF THE STUDY:The increase in the consumption of sushi worldwide attracts attention regarding the microbiological point of view, since it is a ready-to-eat food. To our knowledge, this was the first time that E. coli O157:H7 was identified in sushi samples.

Research -Occurrence of Campylobacter spp., Salmonella spp. and shiga toxin‐producing Escherichia coli in inline milk filters from Swedish dairy farms

Wiley Online

Abstract

This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from 12.6% of filters. The stx genes (stx 1stx 2, or both) were screened from 71% of the filters and STEC was isolated from 14% of these. Of the STEC isolates, 21 contained the stx 1 gene, 19 the stx 2 gene, and five a combination of both stx 1 and stx 2 genes. Whole genome sequence typing on 34 of the 45 STEC showed that they belonged to 21 different serotypes, of which STEC O145:H28 was the most common (2%). STEC O157:H7 was only found from one (0.3%) of the filters. A combination of stx 2 and eae genes was found from 0.7% of the total number of inline milk filters analyzed, while stx 2a was found in 24% of the whole genome‐sequenced isolates. There was a significant positive correlations between number of animals per farm and presence of pathogens on milk filters.

RASFF Alert – STEC E.coli – Boneless Beef

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RASFF -shigatoxin-producing Escherichia coli (stx1/stx2 /25g) in boneless beef from Argentina in Spain

USA -King Arthur Flour Updates Three Lot Codes of Voluntarily Recalled Unbleached All-Purpose Flour (5 lb.)

FDA

King Arthur Flour, Inc. was notified by ADM Milling Co. that three additional product lot codes of Unbleached All-Purpose Flour 5 lb. were omitted from the original data they provided for the press release on October 3, 2019. The additional lot codes and their corresponding “Best Used By” dates are listed below:

Best Used by Date 12/09/19: Lot codes L18A09A L18A09C

Best Used by Date 01/08/20: Lot code A19A08A

This new information only applies to “Best Used By” dates already disclosed in the prior release. No additional Best Used By dates are introduced as a result of these three updated lot codes.

As stated in the prior release, we have undertaken this voluntary recall because of the potential presence of E. coli 026.

King Arthur Flour has not received any confirmed reports of illnesses to date related to this product.

Consumers who have any of these affected products should throw them away and may submit a claim for a refund or replacement at kingarthurflour.com/voluntaryrecallExternal Link Disclaimer, or by calling our King Arthur Flour Consumer Hotline at 866-797-9178.

Consumer safety is our top priority. Consumers are reminded to wash their hands, work surfaces, and utensils thoroughly after contact with raw dough products or flour, and to never eat raw dough or batter. The Centers for Disease Control (CDC) warns consumers to not consume raw products made with flour. E. coli O26 is killed by heat through baking, frying, sautéing, or boiling products made with flour. For more information, refer to the following: https://www.cdc.gov/features/no-raw-dough/index.html.

Bakers have trusted King Arthur Flour products in their kitchens for over 225 years. We remain committed to providing our consumers safe and superior products.

This information can be found online at kingarthurflour.com/voluntaryrecall.External Link Disclaimer Consumers with any questions regarding this recall or King Arthur Flour products are encouraged to call the King Arthur Flour Consumer Hotline at 866-797-9178.

Original Press Release

Research – Survey of Intact and Nonintact Raw Pork Collected at Retail Stores in the Mid-Atlantic Region of the United States for the Seven Regulated Serogroups of Shiga Toxin–Producing Escherichia coli

Journal of Food Protection

ABSTRACT

A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga toxin–producing Escherichia coli (STEC-7) using standard microbiological and molecular methods. In short, 21 (5.3%) of the 395 ground or nonintact pork samples and 3 (3.4%) of the 119 intact pork samples tested positive via the BAX system real-time PCR assay for the stx and eae virulence genes and for the somatic O antigens for at least one of the STEC-7 serogroups. However, none of these 24 presumptive-positive pork samples subsequently yielded a viable isolate of STEC displaying a STEC-7 serogroup-specific surface antigen in combination with the stx and eae genes. These data suggest that cells of STEC serogroups O26, O45, O103, O111, O121, O145, or O157:H7 are not common in retail raw pork samples in the mid-Atlantic region of the United States.

HIGHLIGHTS
  • None of the 514 retail raw pork samples were positive for STEC-7.

  • Four of 514 raw pork samples harbored E. coli of unknown serogroup containing stx and eae.

  • STEC-7 are uncommon in retail raw pork samples in the U.S. mid-Atlantic region.