Category Archives: Food Microbiology Research

Research – Microbiologists get grant to study biofilms guarding foodborne pathogen Listeria

Mirage News

Microbiologists in Penn State’s College of Agricultural Sciences have received a $605,000 grant from the U.S. Department of Agriculture to study how microbial biofilms protect Listeria monocytogenes, the bacterium that causes the deadly foodborne illness listeriosis.

Jasna Kovac, Lester Earl and Veronica Casida Career Development Professor of Food Safety, along with Luke LaBorde, professor of food science, will use the funding from USDA’s National Institute of Food and Agriculture to conduct research on the interactions between microorganisms found in fruit-packing environments and Listeria monocytogenes.

“We will study the ability of environmental microorganisms to form robust biofilms together with L. monocytogenes and how these biofilms may protect L. monocytogenes from the antimicrobial activity of sanitizers,” said Kovac, assistant professor of food science. “The data generated in this project will help improve the cleaning and sanitizing used in the fresh produce industry to better control L. monocytogenes and support the production of safe food.”

Listeria and other microorganisms found in the natural environment, such as soil, can be introduced unintentionally into food-processing facilities with raw foods such as fruit. The research is needed, Kovac explained, because once introduced into the food-processing environment, Listeria and many other environmental microorganisms can grow on surfaces into microbial layers called biofilms.

“Microorganisms enclosed in a biofilm produce slimy substances that protect them from the antimicrobial activity of sanitizing chemicals by slowing down their penetration into the core of a biofilm,” Kovac said. “Biofilm formation can therefore result in reduced efficacy of antimicrobial sanitizers used to inactivate Listeria. This project will investigate the interactions between microorganisms found in fruit-packing environments and L. monocytogenes.”

Research – Determination of Listeria monocytogenes, competitive microflora, microbial hygiene indicators and physico-chemical parameters during the shelf-life of the typical Mediterranean style fermented sausage “Salsiccia Sarda”

Journal of Food Protection

The aim of the present study was the determination of Listeria monocytogenes , competitive microbiota, microbial hygiene indicators and physico-chemical parameters in the typical Mediterranean Style fermented sausages “Salsiccia Sarda” . A batch of “Salsiccia Sarda” (25 samples) naturally contaminated by L. monocytogenes and vacuum packaged after 24 days of ripening was included in the study. Fifteen samples stored at 8° C were analysed after 13, 90 and lastly at the end of shelf-life, after 180 days from vacuum packaging. Ten vacuum packaged samples were stored at 12°C in a domestic fridge simulating temperature abuse and were evaluated at the end of the shelf-life. Samples were subjected to physico-chemical analysis (pH and aw) and investigated for the presence and enumeration of L. monocytogenes . Competitive microbiota, Lactic Acid Bacteria (LAB) and Coagulase negative Staphylococci (CNS), and microbial hygiene indicators (Total mesophilic bacterial counts, Enterobacteriaceae, Enterococcuss spp. and Staphylococcus aureus ) were determined in all the samples. Although a decreasing trend in L. monocytogenes prevalence was observed through the shelf-life, the detection of the pathogen in fermented sausages confirms the ability of L. monocytogenes to overcome the hurdles of the manufacturing process. The results of the present study highlight the importance to carefully evaluate the “Salsiccia Sarda” production process by Food Business Operators (FBOs) in order to maintain unfavourable conditions for the growth of L. monocytogenes .

Research – Prevalence and levels of Campylobacter in broiler chicken batches and carcasses in Ireland in 2017–2018

Science Direct

Highlights

Provides valuable qualitative and quantitative data on Campylobacter in Irish Broilers.

Study demonstrates improvements and progress made in the Irish poultry industry.

Study provides indications of several factors on Campylobacter contamination rates.

Abstract

In 2008, an EU wide baseline survey of broilers revealed a high Campylobacter prevalence. To assist with industry-wide controls, updated data were required. The primary objective of this study was to establish up-to-date data on Campylobacter carriage and carcass contamination in Irish broilers. Monthly samples were collected from the three largest broiler processing plants in Ireland over a twelve-month period. Samples were taken from both first and final thin birds (partial and full depopulation) from 358 batches of broilers. From each batch, a composite sample of 10 caecal contents (n = 358) and 5 neck skins (n = 1790) were collected and numbers of Campylobacter in each sample were determined. Of the 1790 neck skin samples tested, 53% were Campylobacter positive. Campylobacter was detected in the caecal contents of 66% of all batches tested. Depopulation and/or age had a significant effect on Campylobacter prevalence with 67% of final thin broilers yielding Campylobacter-positive neck skin samples in contrast to 38% of first thin broilers that yielded positive neck skin samples (P ≤ 0.002). A significant seasonal variation was observed in the rate of Campylobacter-positive caecal samples with higher prevalence seen in July (85%) than the colder months of November (61%), December (50%), January (61%) March (57%) and April (59%). Neck skin samples were 7 times more likely to be Campylobacter positive if the caecal contents from the same batch were positive (odds ratio = 7.1; P ≤ 0.0001). The decrease in Campylobacter prevalence observed in neck skin and caecal contents demonstrates the improvements and progress made in reducing prevalences of this important enteropathogen in the Irish poultry industry since the 2008 EU baseline survey. It also provides further supporting data on the impact of thinning, the processing environment and season on Campylobacter prevalence.

Sabah – Food poisoning: 10 clusters detected in Sabah during fasting month, only two linked to bazaars

The Star

KOTA KINABALU: Sabah recorded 10 food poisoning clusters involving 189 individuals throughout the fasting month, says Datuk Dr Rose Nani Mudin (pic).

The state Health director said from these cases reported between April 3 and May 1, only two clusters were related to Ramadan bazaars.

“The patients involved have mostly received treatment and recovered from food poisoning,” she said in a statement late Thursday (May 5).

She said none were hospitalised due to food poisoning.

Dr Rose said these cases were detected during ongoing integrated operations on bazaar stalls throughout the fasting month.

She said the main contributors to these cases were unhygienic food handling and food contamination.

“We also conducted activities and operations to monitor the spread of water and food borne transmittable diseases,” she said.

Research – Researchers study use of blue light technology for foodborne outbreaks

National Hog Farmer

Researchers from the University of Georgia Center for Food Safety are beginning a new study to investigate the effectiveness of antimicrobial blue light technology to reduce the foodborne pathogens that cause food poisoning.

When used as a means of disinfecting surfaces in a hospital setting, antimicrobial blue light technology has shown promising results, but little research has yet been done to explore its potential efficacy to control foodborne pathogens.

Through a three-year, $599,900 grant awarded by the U.S. Department of Agriculture’s National Institute of Food and Agriculture, UGA College of Agricultural and Environmental Sciences researchers will test the technology to uncover possible applications in the food production process.

Despite industry efforts, the number of foodborne infections has remained steady and, in some cases, has increased in recent years according to the Centers for Disease Control and Prevention (CDC).

Large-scale outbreaks and recalls still occur, particularly due to environmental contamination — like food preparation and processing surfaces — with Salmonella enterica and Listeria monocytogenes, both of which can cause illness and even death.

Most food manufacturers use chemical sanitizers on food preparation surfaces to help control the spread of foodborne pathogens.

This is typically effective in easy-to-reach areas, but areas that are hard to reach may not receive the same degree of sanitation.

These hard-to-reach places sometimes harbor biofilms, which are clusters of microorganisms such as bacteria that are hard to eliminate. The inadvertent spread of these microorganisms can occur during food production both by humans and machinery. Viruses pose a further challenge as many, such as norovirus, can survive on a variety of surfaces for long periods of time.

India – Food poisoning: E.Coli, Coliforms found in samples from snacks shop

New Indian Express

KASARGOD: The presence of e-Coli and coliform bacteria was found in the food samples taken from Ideal snacks bar at Cheruvathur, said an official source. The Department of Food Safety had sent the samples for testing at the Regional Analytical Laboratory in Kozhikode after a schoolgirl, who had shawarma from the eatery, died, and another 52 persons who ate from there fell sick.

The presence of the highly contagious shigella bacteria, which causes intestinal infection, was found in the blood and stool samples of the patients tested at Kozhikode Medical College Hospital.
The laboratory is now conducting tests on the food samples to ascertain the presence of shigella and salmonella, another bacteria that affect the intestinal tracts, said the source. Only after these two tests, the report would be officially released.

Research – Invasive listeriosis outbreaks and salmon products: a genomic, epidemiological study

Tandfonline

Invasive listeriosis, caused by Listeria (L.) monocytogenes, is a severe foodborne infection, especially for immunocompromised individuals. The aim of our investigation was the identification and analysis of listeriosis outbreaks in Germany with smoked and graved salmon products as the most likely source of infection using whole-genome sequencing (WGS) and patient interviews.

In a national surveillance program, WGS was used for subtyping and core genome multi locus sequence typing (cgMLST) for cluster detection of L. monocytogenes isolates from listeriosis cases as well as food and environmental samples in Germany. Patient interviews were conducted to complement the molecular typing.

We identified 22 independent listeriosis outbreaks occurring between 2010 and 2021 that were most likely associated with the consumption of smoked and graved salmon products. In Germany, 228 cases were identified, of 50 deaths reported (22%) 17 were confirmed to have died from listeriosis. Many of these 22 outbreaks were cross-border outbreaks with further cases in other countries.

This report shows that smoked and graved salmon products contaminated with L. monocytogenes pose a serious risk for listeriosis infection in Germany. Interdisciplinary efforts including WGS and epidemiological investigations were essential to identifying the source of infection. Uncooked salmon products are high risk foods frequently contaminated with L. monocytogenes. In order to minimise the risk of infection for consumers, food producers need to improve hygiene measures and reduce the entry of pathogens into food processing. Furthermore, susceptible individuals should be better informed of the risk of acquiring listeriosis from consuming smoked and graved salmon products’.

Research – Will climate change increase the risk of aflatoxin in U.S. corn?

Science Daily

As climate change continues to alter weather patterns around the planet including the Midwest, researchers at Michigan State University are modeling the impact on crops such as corn.

“The United States is the largest exporter and donor of field corn around the world,” said Felicia Wu, a John A. Hannah Distinguished Professor and an international expert on food safety in the Department of Food Science and Human Nutrition and the Department of Agricultural, Food, and Resource Economics in the College of Agriculture and Natural Resources at MSU. “Here in the U.S., we consume field corn in the form of corn chips, corn flakes, corn grits and corn tortillas; as opposed to sweet corn, which is frozen, canned and eaten off the cob. Field corn is also used for animal feed and for ethanol production.”

The fungi Aspergillus flavus and Aspergillus parasiticus produce aflatoxin which can infect peanuts, tree nuts and corn. Aflatoxin not only degrades corn quality but can also cause health problems for humans and animals depending on the amount and length of time it is ingested. While aflatoxin contamination occurs annually in the southern United States because of the hot and dry climate, it has rarely been a serious problem in the Corn Belt region of the U.S.

“When we ran our near-term climate model scenarios, we found that between 2031-2040, aflatoxin is going to become more of a problem in the U.S. Corn Belt in the Midwest,” Wu said. “The last time there was a serious problem was in 2012 when we had an unusually hot and dry summer throughout the Midwest; particularly Iowa, Illinois and Indiana.”

Wu’s research was published April 5, 2022 in the journal Environmental Research Letters. Co-authors on the paper include her former doctoral student Jina Yu (Hong Kong Baptist University), David Hennessy (Iowa State University) and Jesse Tack (Kansas State University).

Hot and dry conditions encourage fungi spores to be airborne which increases their chances of contaminating crops. Water helps plants withstand stress that makes them vulnerable to harmful fungi. Steps growers, grain elevators and processors can take to reduce the risk of aflatoxin contamination include storing corn harvests in cool, dry conditions and keeping crops irrigated to the extent possible given declining water tables.

Researchers are already using both biotechnological and conventional breeding techniques to develop hybrid crops that can withstand drought, insect damage and fungal infections. In many parts of the world, corn growers are using biocontrol to reduce aflatoxin. Biocontrol infects plants with Aspergillus fungi which is unable to produce aflatoxin because this these fungi competitively exclude the fungi that produce aflatoxin.

Another possibility shifts crop production further north or further south geographically where the climate is cooler or wetter to reduce aflatoxin risk. That, however, impacts farms that have been passed down for generations.

For consumers concerned about eating field corn and their aflatoxin risk, Wu suggests eating your greens and garlic. “Green leafy vegetables are good for your overall health,” Wu said. “Chlorophyll has a sandwich molecular structure that traps aflatoxin molecules so that humans excrete them before the toxin can enter our bloodstream.”

Also, cruciferous vegetables like broccoli, cauliflower, kale and allium vegetables like garlic, onions and leeks can help detoxify carcinogens in our bodies.

“We predict seeing an increase in aflatoxin problems over the next 10-20 years,” Wu said. “So, we need to rely on technologies and a whole suite of interventions that can reduce the problem.”


Story Source:

Materials provided by Michigan State University. Original written by Emilie Lorditch. Note: Content may be edited for style and length.

Spain – Multinational monophasic Salmonella typhimurium outbreak caused by chocolate products

ACSA

This year the Report of the European Center for Disease Prevention and Control (ECDC) and the European Food Safety Authority (EFSA) on this outbreak related to Kinder chocolate products, produced by the Ferrero group, has been published. Given the wide distribution and durability of these products, and the increase in mobility due to the Easter holidays, the outbreak, of 212 cases, affects 5 European countries: France (59 cases), Belgium (54 cases), Germany (18 cases) , the United Kingdom (80 cases) and the Spanish State (1 case). There is even a case in the US.

The first patient was confirmed in the United Kingdom, on January 7, 2022, with a sampling date of December 21, 2021. On February 17, 2022, the United Kingdom, which had already carried out Whole Genome Sequencing (WGS), notified the ECDC of 18 cases of Salmonella typhimurium monophasic BURST Group 1 (eBG1).

Since the elaboration of the ECDC Report, new cases have been detected which are already taken into account in this writing. The ECDC and EFSA are working on an update of the outbreak assessment that is to be published in mid-May.

The most affected population have been children under 10 years of age and women. Many of the affected people have had to be hospitalized since they presented very serious symptoms.

The outbreak strain is resistant to six families of antibiotics: penicillin, aminoglycosides (streptomycin, kanamycin, gentamicin), phenicols, sulfonamides, trimethoprim, and tetracycline. Resistance to aminoglycosides, phenicols, and trimethoprim is rare in monophasic Salmonella typhimurium and can be used for detection of probable cases. It is susceptible to fluoroquinolones, azithromycin, and third-generation cephalosporins.

The chocolate was produced in Belgium and distributed in at least 113 countries. On April 10, 2022, the International Network of Food Safety Authorities (INFOSAN) launched a global alert. Salmonella Typhimurium identified single-phase in the buttermilk tanks of the Ferrero plant in Arlon, in December 2021 and in January 2022. After implementing hygiene measures and obtaining negative tests for salmonella, the Kinder products involved were distributed throughout Europe and throughout the world. However, on April 8, 2022, the Belgian food safety authority reported through RASFF that the investigation at the plant revealed that the management of the contamination carried out by the company did not ensure the healthiness of the products they produced. , for which he ceased its production. It also extended the recall of all batches manufactured at this facility, regardless of batch number and expiration date. The Luxembourg Public Prosecutor’s Office has opened an investigation.It should be noted that Ferrero has production plants in more countries (France, Germany, Poland, etc.), where microbiological tests are also being carried out, given that they share some suppliers. For example, the results obtained in the plant located in Germany are, at this point, all negative, and the cases detected are linked to products made in Belgium. The withdrawal of Ferrero products has been carried out in many countries, including in some where, for the moment, no cases have been declared, such as Canada. and the detected cases are linked to products made in Belgium. The withdrawal of Ferrero products has been carried out in many countries, including in some where, for the moment, no cases have been declared, such as Canada and the detected cases are linked to products made in Belgium. The withdrawal of Ferrero products has been carried out in many countries, including in some where, for the moment, no cases have been declared, such as Canada.

In the Spanish State, on April 6, products with expiration dates between May and August of this year were withdrawn from the market, as a precautionary measure.

Salmonella-contaminated foods are usually not altered in appearance, smell, or taste. 

Belgium – Cora organic dried figs 220 gr – Ochratoxin A

AFSCA

Recall of Cora
Product: Cora organic dried figs 220 gr.
Problem: Too high level of ochratoxin A.

WE ARE RECALLING THE PRODUCTS DESCRIBED BELOW FOLLOWING A NON-COMPLIANCE WHICH MAY LEAD TO A RISK FOR THE CONSUMER:

Product: Cora organic dried figs 220 gr

Barcode: 5400134408133

MDD: 05/25/2022, 05/28/2022 & 06/05/2022

Problem: Too high level of ochratoxin A

These products were sold in Cora Belgium hypermarkets.

CORA has decided, in collaboration with the AFSCA (Federal Agency for the Safety of the Food Chain), to recall these products from consumers.
Customers who have purchased these products are asked not to consume them and to return them to the point of sale, where they will be reimbursed.

In the meantime, the products have been removed from the shelves of all stores.
We sincerely apologize to our customers for these inconveniences and assure them that we are taking the most effective measures to prevent such incidents.

Any customer who wants more information can contact our quality department on 071.69.95.24 or by e-mail at the following address: ALERTES-RETRAITS@CORA.BE .

CORA quality department