Category Archives: Microbiological Risk Assessment

Research – Emerging Method to Protect Food Crops from Carcinogenic Aflatoxins

Food Safety.Com

Researchers from the U.S. Department of Agriculture’s Agricultural Research Service (USDA’s ARS) are using a bioplastic coating to naturally shield seeds from Aspergillus, a type of fungi that produces aflatoxin. Exposure to aflatoxins is a food safety issue due to the compound’s carcinogenic and other harmful effects.

In the U.S., Southern agriculture is most affected by aflatoxins, as hot, dry conditions promote Aspergillus growth and aflatoxin production. Recent science has shown, however, that the Midwestern Corn Belt may be increasingly affected in the near future due to climate change. Corn is highly susceptible to aflatoxin contamination, as are seeds, nuts, feed, stored grain, and other important crops.

The new method for mitigating aflatoxin contamination of crops involves coating seeds with a protective, innocuous strain of Aspergillus, delivered via a mixture of biodegradable, corn starch-based bioplastic and biochar. The competitive Aspergillus strain found in the coating prevents aflatoxin-producing Aspergillus from infecting the seed, and other components of the mixture create a physical barrier that prevents contamination.  

Research – Evaluation of the dietary exposure of the Catalan population to mycotoxins of the genus Fusarium

ACSA

Within the framework of total diet studies, the Catalan Food Safety Agency (ACSA) publishes a second study on the evaluation of mycotoxins: “Evaluation of the dietary exposure of the Catalan population to mycotoxins of the genus Fusarium”.

In 2014, a first study was published in which the presence of the main mycotoxins in food intended for human consumption in the Catalan market was determined, and the intake of food linked to this contamination to estimate the food exposure of the population residing in Catalonia, and evaluate the risk to health (ACSA, 2014). Considering the results obtained, the ACSA considered it necessary to carry out this second, more specific study on mycotoxins, evaluating those that were found most frequently in the foods of the Catalan market, the mycotoxins of the genus Fusarium, and also taking into account the most exposed population groups.

The present study shows that the mycotoxins detected with greater frequency were DON and ENNB followed, with a much lower frequency, by mycotoxin T-2 and mycotoxins FB1 and DON-3G. The rest of mycotoxins analyzed (3-ADON, 15-ADON, NIV, FUS-X, zearalenone, HT-2, FB2 and FB3) will always present levels below the detection limit.

All the age groups evaluated have an exposure to DON and ENNB lower than the respective safety values. The average exposure of the adult population and children in Catalonia to the mycotoxin DON presents values ​​between 7.5% and 10.1% of the safety value. The average exposure of the population to ENNB presents values ​​​​that are two orders of magnitude lower than the extrapolated safety values.

Total diet studies make it possible to better understand the reality linked to the main chemical pollutants that reach the Catalan market, and in this way specific decisions can be made to correct possible risk situations for the health of consumers.

Research – Tofu Whey Wastewater as a Beneficial Supplement to Poultry Farming: Improving Production Performance and Protecting against Salmonella Infection

MDPI

Tofu whey wastewater (TWW) is a by-product of the tofu production process, and contains high amounts of organic products and Lactobacillus ap. However, no studies have been reported on whether naturally fermented TWW can be used as a beneficial additive for poultry production. This study analyzed the main nutritional components and microbial flora of naturally fermented TWW from rural tofu processing plants and their effect on chick production performance, role in modulating the biochemical and immune parameters, and protection against Salmonella enteritidis (S. enteritidis) infection. It was observed that the average pH of TWW was 4.08; therefore, the total viable count was 3.00 × 109 CFU/mL and the abundance of Lactobacillus was 92.50%. Moreover, TWW supplementation increased the total weight gain and feed intake, reduced the feed/gain ratio, increased the length and relative weight of the gut, and reduced the colonization and excretion of S. enteritidis in chickens. Additionally, TWW decreased oxidative damage and pro-inflammatory cytokine secretion caused by S. enteritidis infection. In addition, TWW supplementation ensured the structure of the intestine remained relatively intact in S. enteritidis-infected chicken. Furthermore, TWW markedly promoted the intestinal barrier integrity and up-regulated the relative abundance of Lactobacillus, counteracting the changes in gut microbiota caused by S. enteritidis infection in chicken. In conclusion, our data demonstrated that TWW could be used as a beneficial addition to poultry production, providing a research basis for the further development of TWW as a health care application in in food-producing animal.

Research – Antimicrobial Phage Spray Effective Against Foodborne Bacteria, Including Multidrug Resistant E. Coli

Food Safety.Com

Researchers at McMaster University have developed a new, highly effective tool to mitigate bacterial contamination of foods, including pathogens displaying antimicrobial resistance (AMR). The technology involves the application of bacteriophages (phages)—benign viruses that eat bacteria—to goods in the form of microgels.

Phages are natural predators to bacteria, and because phages attack bacteria in a highly targeted manner, they can be used in food and agriculture without disturbing the balance of microbial communities. Phage products have been approved by the US Food and Drug Administration (FDA) for controlling dangerous bacterial contaminants such as Escherichia coli in food products. Though they do not affect the taste, texture, and nutritional quality of foods, phages are not widely used by industry due to challenges with delivery and stability of phage products.

Research -Risk assessment of enteric viruses along the food chain and in the population

EFSA

Abstract

Food-borne microbial illness contributes up to one third of global disease burden. The largest category of food-borne illness is gastroenteritis, the majority of which is caused by enteric viruses. Viruses like these are transmitted to food either by waste-contaminated waters, or by handling and transfer during processing.

An important tool for reducing or controlling food-borne microbial risk is risk analysis. This framework has been adopted globally to manage risks associated with microbial contamination in food. Several hundred microbial risk assessments (MRAs) have been published by different national and international organisations, for different food-hazard combinations. The use of MRAs in controlling and understanding virus risk has, to date, been limited, compared with the efforts made on bacterial pathogens. Given the large disease burden that viruses are responsible for, this disparity should be addressed. 

The main reasons for the relative lack of risk assessments are the difficulty in detecting and monitoring viruses compared with bacteria. This means less data on prevalence, concentration and inactivation, and allows viruses to remain silent contributors to global disease. There are also key conceptual differences between virus risk assessment and bacterial risk assessment. This project aimed to assess the current state of the art for food-borne virus risk assessment, then to progress the field further by using the data available to produce risk rankings and risk assessments.

This was done by a combination of literature reviewing and various risk assessment tools. The result was an assessment of the overall evidence base in the literature, a semi-quantitative ranking comparison between the viruses and foods of most concern, and a survey of inactivation methods, leading to a quantitative ranking of the effectiveness of each in reducing and managing food-borne virus risk.

Research – Identification of Genetic Markers for the Detection of Bacillus thuringiensis Strains of Interest for Food Safety

MDPI

Abstract

Bacillus thuringiensis (Bt), belonging to the Bacillus cereus (Bc) group, is commonly used as a biopesticide worldwide due to its ability to produce insecticidal crystals during sporulation. The use of Bt, especially subspecies aizawai and kurstaki, to control pests such as Lepidoptera, generally involves spraying mixtures containing spores and crystals on crops intended for human consumption. Recent studies have suggested that the consumption of commercial Bt strains may be responsible for foodborne outbreaks (FBOs). However, its genetic proximity to Bc strains has hindered the development of routine tests to discriminate Bt from other Bc, especially Bacillus cereus sensu stricto (Bc ss), well known for its involvement in FBOs. Here, to develop tools for the detection and the discrimination of Bt in food, we carried out a genome-wide association study (GWAS) on 286 complete genomes of Bc group strains to identify and validate in silico new molecular markers specific to different Bt subtypes. The analyses led to the determination and the in silico validation of 128 molecular markers specific to Bt, its subspecies aizawaikurstaki and four previously described proximity clusters associated with these subspecies. We developed a command line tool based on a 14-marker workflow, to carry out a computational search for Bt-related markers from a putative Bc genome, thereby facilitating the detection of Bt of interest for food safety, especially in the context of FBOs.

Australia – Cause of illnesses from spinach in Australia identified; Costco among stores that received product

Food Safety News

Investigations into almost 200 foodborne illnesses in Australia after eating a brand of baby spinach have revealed what caused the illnesses.

Riviera Farms said the spinach was contaminated with a weed called thornapple. The scientific name is Datura stramonium and it is also known as jimsonweed. How the weed got into the food supply is still being investigated by Victorian authorities with site inspections underway.

Riviera Farms issued a recall of baby spinach after reports of customers falling ill. The company then contacted its 20 clients. Costco is the only direct major retail client, however, the product was also sold to stores such as Coles, Aldi, and Woolworths.

Spinach products were grown on a farm in Victoria and shipped to several stores across the country. More than 190 potential cases were reported in New South Wales, Australian Capital Territory, Victoria, and Queensland.

There have been several hospitalizations. Toxicological impacts are still to be confirmed but it is understood most people experienced symptoms for a short time and then recovered.

RASFF Alert – Animal Feed – Aflatoxin – Corn Gluten

RASFF

Aflatoxin B1 in corn gluten from Austria in Germany and Switzerland

RASFF Alerts – Animal Feed – Salmonella

RASFF

Salmonella Enteritidis in potato starch for feed from Belgium in the Netherlands

RASFF

Salmonella in complete feed for dogs from Netherlands in Belgium

RASFF

Salmonella in fishmeal from Spain in Belgium

The Handwashing Handbook

Global Handwashing Org

The Handwashing Handbook is based on the experience of the Global Handwashing Partnership and presents best practices and new concepts to improve the uptake of handwashing. The Handwashing Handbook focuses on:

  • Making the Case for Handwashing
  • Designing and Implementing Handwashing Programs
  • Improving Handwashing in Specific Contexts
  • Addressing Handwashing at a Systems Level

The Handwashing Handbook is available in Arabic, Chinese, English, French, Portuguese, and Spanish.

Resource Attachments:
https://globalhandwashing.org/wp-content/uploads/2020/10/GHP_Handwashing-Handbook_FINAL.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Arabic.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Chinese.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-French.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Portuguese.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Spanish.pdf (pdf)