Category Archives: Research

Research – Development of Desiccation-Tolerant Probiotic Biofilms Inhibitory for Growth of Foodborne Pathogens on Stainless Steel Surfaces

MDPI

Lactic acid bacteria biofilms can be used to reduce foodborne pathogen contamination in the food industry. However, studies on growth inhibition of foodborne pathogens by inducing biofilm formation of antagonistic microorganisms on abiotic surfaces are rare. We developed a desiccation-tolerant antimicrobial probiotic biofilm. Lactobacillus sakei M129-1 and Pediococcus pentosaceus M132-2 isolated from fermented Korean foods were found to exhibit broad-spectrum antibacterial activity against Bacillus cereusEscherichia coli O157:H7, Staphylococcus aureusListeria monocytogenes, and Salmonella enterica. Their biofilm levels were significantly (p < 0.05) higher on stainless steel than on polyethylene or ceramic. Biofilms of both isolates showed significantly (p < 0.05) enhanced resistance against desiccation (exposure to 43% atmospheric relative humidity) as compared with the isolates not in the biofilm form. The antimicrobial activity of the isolates was sustained in dried biofilms on stainless steel surface; the initial number of foodborne pathogens (average 7.0 log CFU/mL), inoculated on stainless steel chips containing L. sakei M129-1 or P. pentosaceus M132-2 biofilm decreased to less than 1.0 log CFU within 48 h. The lactic acid bacteria antibacterial biofilms developed in this study may be applied to desiccated environmental surfaces in food-related environments to improve microbiological food safety. View Full-Text

UK – Several supermarkets improve Campylobacter in chicken results

Food Safety News

The supermarket Marks and Spencer has reported better results for Campylobacter in chicken in the latest quarterly figures from the United Kingdom.

The data covers October to December 2021 for nine retailers on high levels of Campylobacter in fresh, shop-bought, UK-produced chickens.

Results at Morrisons, Lidl, Waitrose and Sainsbury’s went up while Marks and Spencer, Tesco, Co-op, Aldi and Asda recorded lower levels of contamination compared to the previous quarter.

The Food Standards Agency (FSA) maximum level is 7 percent of birds with more than 1,000 colony forming units per gram (CFU/g) of Campylobacter.

Research – Salmonella and Campylobacter continue to show high levels of antibiotic resistance

EFSA

Antibiotic resistance in Salmonella and Campylobacter bacteria is still high, says a report released today by the European Centre for Disease Prevention and Control (ECDC) and the European Food Safety Authority (EFSA).

Campylobacteriosis was the most reported zoonosis in the EU in 2020 and the most frequently reported cause of foodborne illness. Campylobacter bacteria from humans and poultry continues to show very high resistance to ciprofloxacin, a fluoroquinolone antibiotic, that is commonly used to treat some types of bacterial human infection.

Increasing trends of resistance against the fluoroquinolone class of antibiotics has been observed in humans and broilers for Campylobacter jejuni. In Salmonella Enteritidis, the most common type of Salmonella in humans, increasing trends of resistance to the quinolone/fluoroquinolone class of antibiotics were observed. In animals, resistance to these antibiotics in Campylobacter jejuni and Salmonella Enteritidis were generally moderate to high.

However, despite the increasing trends of resistance against certain antibiotics, simultaneous resistance to two critically important antibiotics – remains low for E. coliSalmonella and Campylobacter in bacteria from both humans and food-producing animals.

A decline in resistance to tetracyclines and ampicillin in Salmonella from humans was observed in nine and ten countries, respectively, over the period 2016-2020, and this was particularly evident in Salmonella Typhimurium. Despite the decline, resistance to these antibiotics still remains high in bacteria from both humans and animals.

Furthermore, in more than half of the European Union countries, a statistically significant decreasing trend in the prevalence of extended-spectrum β-lactamase (ESBL)-producing E. coli was observed in food-producing animals. This is an important finding as particular strains of ESBL-producing E. coli are responsible for serious infections in humans.

Carbapenem resistance remains extremely rare in E. coli and Salmonella from food-producing animals. Carbapenems are a class of last resort antibiotics and any findings showing resistance to these in zoonotic bacteria are concerning.

Although findings and trends are consistent with data reported in previous years, the COVID-19 pandemic had an impact on the amount of data reported, particularly with regards to public health.

An interactive data visualisation tool shows resistance levels in humans, animals and food, country-by-country in 2019 and 2020.

Additionally, the human food and waterborne antibiotic resistance data is published in ECDC’s Surveillance Atlas of Infectious Diseases (under the diseases campylobacteriosis, salmonellosis and shigellosis, respectively).

Norway – Analyzes of Pathogenic Escherichia coli in bacon sausages 2022

Mattilsynet

On behalf of the Norwegian Food Safety Authority, the Norwegian Veterinary Institute has examined 137 Norwegian bacon sausages for pathogenic E. coli bacteria (STEC). There was a discovery of such bacteria, but this type does not usually cause serious illness in humans. Although the sample was small, the conclusion is that the prevalence of STEC in Norwegian bacon sausages is low.

Mattilsynet

What we investigated: 137 Norwegian bacon sausages
Period: The samples were obtained in 2020 and analyzed in 2021
What we were looking for: E. coli and pathogenic STEC variants
What we found: A positive E. coli sample. This was not the type that usually causes serious illness in people, ie good results.
Who carried out the assignment: Veterinary Institute
Escherichia coli is present in the gastro intestinal tract of humans and warm-blooded animals, and are usually harmless. However, some groups of E.coli may cause infections in humans. Shiga toxin-producing E. coli (STEC) is one of the groups of pathogenic E. coli. Ruminants, in particular cattle, are assumed to be the main reservoir for STEC.
The results from previous surveys of cattle and sheep in Norway for the occurrence of STEC of the serogroups most often associated with infection, have indicated a low occurrence. Similar results were observed in a survey of minced meat from cattle, on the Norwegian market. The Norwegian Food Safety Authority commissioned a survey of STEC in domestically produced fermented sausages. The samples were collected in 2020 with subsequent analysis in 2021.
A total of 137 samples of domestically produced fermented sausage were collected and analysed. Enriched samples were examined for the presence of the genetic markers stx1, stx2 and eae. Attempts of isolation were carried out from samples that were positive for one or more of the virulence markers. Isolates identified as STEC were further characterized using whole genome sequencing.
STEC was isolated from only one sample, and the isolate was characterized as STEC O76:H19 harbouring stx1c, but did not carry eae. In the primary screening of enriched samples, only a few samples were positive for the virulence markers. The results from this study indicates that the occurrence of STEC in
Norwegian fermented sausages is low.
Although STEC was isolated, none of the serogroups associated with severe infections were isolated. It must be taken into account that a low number of samples were analysed. Since this type of products has not been analysed previously, the present survey provides knowledge for the industry, authorities and knowledge institutions. It is important to carry out similar surveys regularly to generate updated Norwegian data.

France – Report on surveillance of Campylobacter infections in France in 2020.

Sante Publique

Key points

In 2020, surveillance of Campylobacter infections confirmed the epidemiological and biological trends already observed in recent years: 

  • a predominance of the species C. jejuni ;
  • higher number of cases and incidence in children;
  • a predominance of infections in men, except in people aged 30 to 39;
  • a seasonal peak during the summer period;
  • high resistance to fluoroquinolones and tetracyclines, which has remained stable in recent years;
  • no notable increase in the resistance rates of the six antibiotics tested routinely;
  • the consumption of poultry as the first food (incriminated or suspected) source of contamination in episodes of collective food poisoning.

The number of Campylobacter strains listed by the CNR has been increasing since 2013, the year in which the network’s laboratories introduced online data entry. This increase could be a reflection of an increase in Campylobacter infections in France. However, this increase in the number of strains must be considered within the framework of the specificities of the surveillance system. Several factors, such as an increase in the activity of the network’s laboratories or prescriptions for stool cultures, could cause an increase in the number of isolations and notifications over time. The implementation of multiplex PCR in many laboratories has also facilitated the detection of Campylobacter sp in stool samples.

The health context linked to the COVID-19 pandemic does not seem to have had an impact on the surveillance data. A decrease in the number of strains compared to previous years was observed only in March-April 2020, corresponding to the period of the first confinement. This reduction seems to reflect less recourse to care (medical consultation, biological analyses) during this period, but could also indicate a reduction in the incidence linked to health restrictions.

USA – CDC – Lettuce, Other Leafy Greens, and Food Safety

CDC

Leafy greens arranged on a white background

Vegetables are an important part of a healthy diet. Leafy vegetables (called leafy greens on this page), such as lettuce, spinach, cabbage, kale, and bok choy, provide nutrients that help protect you from heart diseasestroke, and some cancers.

But leafy greens, like other vegetables and fruits, are sometimes contaminated with harmful germs. Washing leafy greens does not remove all germs. That’s because germs can stick to the surface of leaves and even get inside them. If you eat contaminated leafy greens without cooking them first, such as in a salad or on a sandwich, you might get sick.

Although anyone can get a foodborne illness, sometimes called food poisoning, some groups of people are more likely to get one and to have a serious illness. These groups include:

  • Adults aged 65 and older
  • Children younger than 5 years
  • People who have health problems or take medicines that lower the body’s ability to fight germs and sickness (a weakened immune system)external icon
  • Pregnant people

Eating Leafy Greens

Are leafy greens safe to eat?

Millions of servings of leafy greens are eaten safely every day in the United States. But leafy greens are occasionally contaminated enough to make people sick. To reduce your chance of getting sick, always follow the steps for safely handling and preparing leafy greens before eating or serving them.

Are leafy greens safe for my pet to eat?

Some animals can get sick from some germs that also make people sick. Always follow the steps for safely handling and preparing leafy greens before feeding them to pets and other animals. Never feed recalled food to pets or other animals.

Safely Handling and Preparing Leafy Greens

Do I need to wash all leafy greens?

Prewashed greens don’t need to be washed again. If the label on a leafy greens package says any of the following, you don’t need to wash the greens:

  • Ready-to-eat
  • Triple washed
  • No washing necessary

Prewashed greens sometimes cause illness. But the commercial washing process removes most of the contamination that can be removed by washing.

All other leafy greens should be thoroughly washed before eating, cutting, or cooking.

What is the best way to wash leafy greens?

The best way to wash leafy greens is by rinsing them under running water. Studies show that this step removes some of the germs and dirt on leafy greens and other vegetables and fruits. But no washing method can remove all germs.

Follow these steps to wash leafy greens that you plan to eat raw:

  • Wash your hands for 20 seconds with soap and water before and after preparing leafy greens.
  • Get rid of any torn or bruised leaves. Also, get rid of the outer leaves of cabbages and lettuce heads.
  • Rinse the remaining leaves under running water. Use your hands to gently rub them to help get rid of germs and dirt.
  • Dry leafy greens with a clean cloth or paper towel.

Should I soak leafy greens before washing them?

No. Do not soak leafy greens. If you soak them in a sink, germs in the sink can contaminate the greens. If you soak them in a bowl, germs on one leaf can spread to the other leaves. Rinsing leafy greens under running water is the best way to wash them.

Should I wash leafy greens with vinegar, lemon juice, soap, detergent, or produce wash?

Use plain running water to wash leafy greens and other produce. Kitchen vinegar and lemon juice may be used, but CDC is not aware of studies that show vinegar or lemon juice are any better than plain running water.

Do not wash leafy greens or other produce with soap, detergent, or produce wash. Do not use a bleach solution or other disinfectant to wash produce.

What other food safety steps should I keep in mind when I select, store, and prepare leafy greens and other produce?

  • Select leafy greens and other vegetables and fruits that aren’t bruised or damaged.
  • Make sure pre-cut produce, such as bagged salad or cut fruits and vegetables, is refrigerated or on ice at the store.
  • Separate produce from raw meat, poultry, seafood, and eggs in your shopping cart, grocery bags, and refrigerator.
  • Store leafy greens, salads, and all pre-cut and packaged produce in a clean refrigerator with the temperature set to 40°F or colder.
  • Use separate cutting boards and utensils for produce and for raw meat, poultry, seafood, and eggs. If that isn’t an option, prepare produce before working with raw meat.
  • Wash utensils, cutting boards, and kitchen surfaces with hot, soapy water after each use.
  • Cook thoroughly or throw away any produce that touches raw meat, poultry, seafood or their juices.
  • Refrigerate cooked or cut produce, including salads, within 2 hours (1 hour if the food is exposed to temperatures above 90°F, like a hot car or picnic).

Germs, Outbreaks, and Recalls

How do leafy greens get contaminated with germs?

Germs that make people sick can be found in many places, including in the soil, in the feces or poop of animals, in refrigerators, and on kitchen surfaces.

Germs can contaminate leafy greens at many points before they reach your plate. For example, germs from animal poop can get in irrigation water or fields where theexternal icon vegetables grow. Germs can also get on leafy greens in packing and processing facilities, in trucks used for shipping, from the unwashed hands of food handlers, and in the kitchen. To prevent contamination, leafy greens should be grown and handled safely at all points from farm to fork.

Read a study by CDC and partners on what we have learned from 10 years of investigating E. coli outbreaks linked to leafy greens.

How common are outbreaks linked to leafy greens?

In 2014–2018, a total of 51 foodborne disease outbreaks linked to leafy greens (mainly lettuce) were reported to CDC. Five of the 51 were multistate outbreaks that led CDC to warn the public. Among those five outbreaks, two were linked to packaged salads, two were linked to romaine lettuce, and one could not be linked to a specific type of leafy greens.

Most recently, in 2019–2021, CDC investigated and warned the public about nine multistate outbreaks linked to leafy greens. Among those outbreaks, six were linked to packaged salads, one was linked to romaine lettuce, one was linked to baby spinach, and one could not be linked to a specific type of leafy greens. Learn about these outbreaks.

Most foodborne illnesses are not part of a recognized outbreak. The nearly 2,000 illnesses reported in 2014–2020 outbreaks linked to leafy greens represent only a small part of illnesses caused by contaminated leafy greens during those years.

Does CDC warn the public about every foodborne disease outbreak?

No. CDC does not warn the public about every foodborne outbreak—including ones linked to leafy greens. Some reasons for this include:

  • Most sources of foodborne outbreaks are never identified.
  • By the time a source is identified, it might no longer be in stores, restaurants, or homes. This can happen with foods that are perishable (foods that spoil or go bad quickly), such as leafy greens.
  • Most outbreaks affect people in only one state, so local or state health departments lead the work to identify, investigate, and communicate about those outbreaks. CDC typically communicates only about outbreaks that affect people in more than one state.

Investigating outbreaks linked to leafy greensexternal icon can be especially challenging. These outbreaks often go unidentified or unsolved.

What should I do with leafy greens that are part of a recall?

  • Never eat, serve, or sell food that has been recalled, even if some of it was eaten and no one got sick.
  • Return the recalled food to the store or throw it away at home.
    • Throw out the recalled food and any other foods stored with it or that touched it.
    • Put it in a sealed bag in an outside garbage can with a tight lid (so animals cannot get to it).
    • If the recalled food was stored in a reusable container, wash the container in the dishwasher or with hot, soapy water.
  • Follow CDC’s instructions for cleaning your refrigerator after a food recall.

Organic, Hydroponic, and Home-Grown Leafy Greens

Are organic leafy greens less likely to be contaminated than non-organic ones?

All kinds of produce, including organic leafy greens, can be contaminated with harmful germs at any point from farm to fork. CDC is not aware of any evidence that organic greens are safer.

Learn about some outbreaks linked to organic foodsexternal icon.

Are hydroponic or greenhouse-grown leafy greens less likely to be contaminated?

Leafy greens grown using these methods also can be contaminated with harmful germs at any point from farm to fork.

Learn about an outbreak linked to greenhouse-grown leafy greens.

How do I keep leafy greens in my garden safe to eat?

Home gardens can be an excellent source of fruits and vegetables. Follow these tips to help prevent food poisoning:

  • Plant your garden away from animal pens, compost bins, and manure piles.
  • Water your garden with clean, drinkable water.
  • Keep dirty water, including storm runoff, away from the parts of plants you will eat.

Learn about raised bed gardening pdf icon[PDF – 1 page].

Looking to the Future

What steps are industry and the government taking to make leafy greens safer?

CDC is collaborating with FDA, academia, and industry to investigate the factors that contribute to leafy greens contamination.

The leafy greens industry, FDA, and state regulatory authorities have been implementing provisions of the Produce Safety Ruleexternal icon as part of the FDA Food Safety Modernization Act (FSMA).external icon They are considering what further measures can be taken. FDA’s 2020 Leafy Greens STEC Action Planexternal icon describes the agency’s plans to work with partners to make leafy greens safer.

Research – Levels and genotypes of Salmonella and levels of Escherichia coli in frozen ready-to-cook chicken and turkey products in England tested in 2020 in relation to an outbreak of S. Enteritidis

Science Direct

Highlights

Six serovars, with S. Infantis and S. Enteritidis most common, found in 9% of samples

Serovar-specific PCR and Cragie’s motility method used for co-contaminated samples

All S. Enteritidis were outbreak strains and affected six products from two plants.

The highest MPN/g was 54 for S. Infantis and 28 and S. Enteritidis.

Detection of Salmonella spp. was associated with higher levels of generic E. coli.

Abstract

Frozen reformulated (FR) breaded chicken products have previously been implicated in causing human salmonellosis. A multi-country Salmonella enterica serovar Enteritidis outbreak involving several strains with >400 reported human cases in the UK occurred in 2020. Initially S. Infantis was detected in one sample from a case home but S. Enteritidis was then also isolated using a S. Enteritidis specific PCR in combination with isolation via a Craigie-tube. This prompted a survey to examine the presence and levels of Salmonella and E. coli in ready-to-cook FR poultry products in England in 2020.

From a total of 483 samples, including two from cases’ homes, Salmonella was detected in 42 chicken samples, these originated from six out of 53 production plants recorded. Salmonella detection was associated with elevated levels of generic E. coli (OR = 6.63). S. Enteritidis was detected in 17 samples, S. Infantis in 25, S. Newport in four and S. Java, S. Livingstone and S. Senftenberg in one each. The highest levels of Salmonella were 54 MPN/g for S. Infantis and 28 MPN/g for S. Enteritidis; 60% of the Salmonella-positive samples had <1.0 MPN/g. S. Enteritidis was detected together with S. Infantis in five samples and with S. Livingstone in one. Where S. Enteritidis was detected with other Salmonella, the former was present at between 2 and 100-fold lower concentrations. The Salmonella contamination was homogeneously distributed amongst chicken pieces from a single pack and present in both the outer coating and inner content. The S. Enteritidis were all outbreak strains and detected in six products that were linked to four production plants which implicated a Polish origin of contamination. Despite S. Infantis being most prevalent in these products, S. Infantis from only two contemporaneous human cases in the UK fell into the same cluster as isolates detected in one product. Except for one human case falling into the same cluster as one of the S. Newport strains from the chicken, no further isolates from human cases fell into clusters with any of the other serovars detected in the chicken samples.

This study found that higher E. coli levels indicated a higher probability of Salmonella contamination in FR chicken products. The results also highlight the importance of recognising co-contamination of foods with multiple Salmonella types and has provided essential information for detecting and understanding outbreaks where multiple strains are involved.

USA – Webinar on the Foodborne Outbreak Response Improvement Plan – April 13th 2022

FDA

New Era for Smarter Food Safety

The U.S. Food and Drug Administration (FDA) is holding a webinar on the Foodborne Outbreak Response Improvement Plan that the agency released in early December 2021.

Deputy FDA Commissioner Frank Yiannas and FDA experts across agency’s human foods program will be available to explain and answer questions about the plan with the goal of raising awareness, enhancing understanding, and building support. Stakeholders are welcome to provide their insights as well as ask questions.

This response improvement plan focuses on tech-enabled product traceback, root cause investigations, analysis and dissemination of outbreak data, and operational improvements. It is intended to work in concert with FDA’s New Era of Smarter Food Safety Blueprint, which outlines specific approaches the FDA will take over the next decade to address food safety in the rapidly changing food system.

The plan was also informed by an independent review of the FDA’s structural and functional capacity to support, participate in, or lead multistate foodborne illness outbreak investigation activities. You will hear more about that review in this webinar.

The speakers will be:

  • Frank Yiannas, Deputy Commissioner for Food Policy and Response
  • RADM David Goldman, Chief Medical Officer, Office of Food Policy and Response
  • Stic Harris, Director, FDA’s Coordinated Response and Evaluation Network (CORE)
  • CDR Kari Irvin, Deputy Director, CORE
  • Scott MacIntire, Program Director, Office of Human and Animal Food Operations – West
  • Craig Hedberg, University of Minnesota, author of “An Independent Review of FDA’s Foodborne Outbreak Response Processes”

The webinar will also be recorded and posted to the FDA website.

You are also welcome to submit questions during the webinar to SmarterFoodSafety@fda.hhs.gov.

Registration

Registration is required. There will be an opportunity to ask questions in advance on the registration form.

REGISTERExternal Link Disclaimer

Additional Information

USA – New Era of Smarter Food Safety: FDA’s Foodborne Outbreak Response Improvement Plan

I. What progress have we made to date?

The FDA and its par

FDA

New Era of Smarter Food Safety - FDA's Foodborne Outbreak Response Improvement Plan Cover

Tackling foodborne outbreaks faster and revealing the root cause are essential for the prevention of future outbreaks. We have a plan to do that.

Foodborne disease remains a significant public health problem in the United States. The FDA’s Foodborne Outbreak Response Improvement Plan (FORIP), described in this document, is an important step that the FDA is taking to enhance the speed, effectiveness, coordination, and communication of outbreak investigations. (Unless stated otherwise, this report focuses exclusively on the response to human food and not animal food.)

Our ultimate goal is to bend the curve of foodborne illness in this country.

 

USA – Microbiological Surveillance Sampling: FY17–19 Processed Avocado and Guacamole

FDA

The U.S. Food and Drug Administration collected and tested processed avocado, the main ingredient in guacamole, and finished guacamole as part of the agency’s proactive and preventive approach to deploying its sampling resources with the ultimate goal of preventing contaminated food from reaching consumers.

Assignment Overview

The assignment began in November 2017 and ended in September 2019. In total, the FDA collected and tested 887 samples of processed avocado and guacamole (domestic and imported product) for Salmonella spp. and Listeria monocytogenes. This total is smaller than the initial number of samples the agency set out to collect and test because the agency encountered factors that twice required a reduction of the collection target, as explained in the Sample Collection section of this report (page 6).

As to the design of the assignment, the FDA directed its field staff not to collect products that had undergone high-pressure processing (HPP) or products intended for HPP. HPP is a “kill step” validated to eliminate pathogenic microorganisms in food, and it is often used in the manufacture of processed avocado and guacamole. In seeking to exclude from the assignment products that had been HPP-treated, the FDA’s intent was to focus on products that posed the greatest risk to consumers.

The agency learned during its evaluation of the test results that some of the products collected had received HPP treatment but were not labeled as such. FDA staff worked retrospectively with industry to identify the HPP-treatment status of the samples collected but could not determine the status of a number of samples. Those samples were designated as “could not ascertain” for purposes of the data analysis.

Findings and Follow-up Actions

The FDA detected Salmonella spp.in two samples which were later determined to be distinct samples of the same brand of domestically manufactured guacamole from different lots. Neither sample had received HPP treatment. In addition, the agency detected Listeria monocytogenes in 15 samples from nine different firms. Of those 15 samples, eight had not been HPP treated. The HPP-treatment status of the other seven samples could not be ascertained.

When the FDA detected a pathogen in a domestic sample, agency personnel worked with the company that owned or distributed the affected product to conduct a voluntary recall in all cases in which product was available, or likely to still be available, to consumers. The FDA also conducted one follow-up inspection of a domestic facility, and state officials in Florida likewise conducted one domestic inspection. As to the imported samples, the agency refused to admit lots associated with the positives and placed the responsible companies on import alert. In all, the agency placed two firms on import alert. In addition, the agency conducted whole genome sequencing (WGS) analysis on the positives but was unable to determine whether processed avocado or guacamole were the food vehicle associated with any known human illnesses.

In addition to affirming that Salmonella spp. and Listeria monocytogenes may be present in processed avocado and/or guacamole, the assignment data show that the estimated prevalence of these pathogens in the non-HPP-treated samples was higher than in the HPP-treated samples. This finding appears to support other research that shows HPP is effective at neutralizing pathogenic microorganisms,[1] even as this assignment was not designed to compare possible differences based on HPP-treatment status. The findings also underscore the need for processors and others in the processed avocado and guacamole supply chain to comply with the FDA’s Preventive Controls for Human Food Rule[2] and for importers of these foods to comply with the FDA’s Foreign Supplier Verification Programs Rule.[3]