Category Archives: Research

Research – Quantitative Microbial Risk Assessment of Listeria monocytogenes and Enterohemorrhagic Escherichia coli in Yogurt

Posted on April 1, 2022 by | Leave a comment

MDPI

Listeria monocytogenes can survive in yogurt stored at a refrigeration temperature. Enterohemorrhagic Escherichia coli (EHEC) has a strong acid resistance that can survive in the yogurt with a low pH. We estimated the risk of L. monocytogenes and EHEC due to yogurt consumption with @Risk. Predictive survival models for L. monocytogenes and EHEC in drinking and regular yogurt were developed at 4, 10, 17, 25, and 36 °C, and the survival of both pathogens in yogurt was predicted during distribution and storage at home. The average initial contamination level in drinking and regular yogurt was calculated to be −3.941 log CFU/g and −3.608 log CFU/g, respectively, and the contamination level of both LM and EHEC decreased in yogurt from the market to home. Mean values of the possibility of illness caused by EHEC were higher (drinking: 1.44 × 10−8; regular: 5.09 × 10−9) than L. monocytogenes (drinking: 1.91 × 10−15; regular: 2.87 × 10−16) in the susceptible population. Both pathogens had a positive correlation with the initial contamination level and consumption. These results show that the foodborne illness risk from L. monocytogenes and EHEC due to yogurt consumption is very low. However, controlling the initial contamination level of EHEC during yogurt manufacture should be emphasized.

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Posted in Decontamination Microbial, EHEC, escherichia coli, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Listeria, Listeria monocytogenes, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Research

Research – Plant Extract and Essential Oil Application against Food-Borne Pathogens in Raw Pork Meat

Posted on April 1, 2022 by | Leave a comment

MDPI

Herbal and plant extracts are being applied for a wide range of foods against different types of food-borne pathogens. In the present study, ethanolic and aqueous extracts (2% w/v) from cranberry (Vaccinium macrocarpon) and pomegranate (Punica granatum L.) plants were applied alone or in combination with two essential oils (thyme and oregano in a concentration of 0.150 μg/g) in pork meatballs and their antimicrobial activity was estimated. The extracts exhibited promising results (aqueous and ethanolic extracts of pomegranate and cranberry in a food-compatible concentration of 2% w/v) were applied to raw pork meatball production and their antimicrobial activity was recorded versus Enterobacteriaceae, total mesophilic bacteria, yeasts/molds, Staphylococcus spp., Pseudomonas spp. and lactic acid bacteria (LAB). The outcome demonstrated that meatballs containing aqueous extracts of pomegranate were more resistant to spoilage compared to all the other samples since they were preserved for more days. The chemical profiles of plant extracts were determined through LC-QTOF/MS and the chemical composition of the essential oils applied was determined with the use of GC/MS in order to identify the substances involved in the observed antimicrobial activity. Phenolic acids (quinic acid, chlorogenic acid), monoterpenes (p-cymene, carvacrol, thymol, limonene), organic acids (citric acid) and phenols were the main constituents found in the plant extracts and essential oils applied. These extracts of plant origin could be used as natural preservatives in meat products, even in low concentrations. View Full-Text

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Posted in Decontamination Microbial, Enterobacteriaceae, Food Micro Blog, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, lactic acid bacteria, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Pseudomonas, Research, Staphylococcus aureus

Food poisoning or hangover? Here’s how you can tell the difference

Posted on April 1, 2022 by | Leave a comment

Food Poisoning News

When you drink too much alcohol at night, you may wake up feeling sick the next day. The majority of people assume it’s another awful hangover, but there’s a chance that food poisoning or even alcohol poisoning could be to blame. Because both food poisoning and a hangover might induce nausea, vomiting, and headaches, determining whether you’re experiencing one of them can be tough.

A few key characteristics can help you figure out if it’s food poisoning or a hangover. If you have a fever, chills, and body aches, it’s more likely to be food poisoning. If you have a throbbing headache and feel very thirsty with a dry mouth, it’s more likely to be a hangover. Other symptoms like diarrhea are common to both conditions.

Let’s look into both hangovers and food poisoning in further detail at the link above.

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Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Research

Research – Vehicle Windshield Wiper Fluid as Potential Source of Sporadic Legionnaires’ Disease in Commercial Truck Drivers

Posted on March 31, 2022 by | Leave a comment

CDC

Abstract

Sporadic Legionnaires’ disease is frequently detected in commercial truck drivers. We report 2 sporadic cases of this disease in Barcelona, Spain, that occurred during December 2019 and September 2020. Laboratory findings were consistent with windshield wiper fluid without added screen wash as a possible source of infection for both cases.

Legionnaires’ disease is a severe form of acute pneumonia caused by inhalation of aerosols containing Legionella bacteria. Most Legionella infections are related to contaminated artificial water systems. Systems with warm water (35°C), stagnation, and lack of disinfection and maintenance can lead to proliferation of Legionella spp. . Cooling towers, warm water systems, and whirlpool spas are well-established sources of infection . However, in most sporadic cases, the source of infection remains unknown.

Commercial truck drivers are at increased risk for Legionnaires’ disease. Exposures related to the vehicle are usually considered secondary to outside sources in industrial areas, such as cooling towers, and are seldomley investigated, despite some studies suggesting them as potential sources. Using windshield wiper fluid without added screen wash has been identified as a risk factor for Legionnaires’ disease in commercial drivers in a previous case‒control study. In addition, Legionella spp. can grow in windshield wiper fluid that does not contain screen wash. However, no studies have epidemiologically confirmed the fluid as the source of infection. We report 2 cases of Legionnaires’ disease cases diagnosed by urine antigen testing (UAT) linked to detection of the bacteria in the windshield wiper fluid.

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Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology Blog, Food Microbiology Research, Legionella, Legionnaires’ disease, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Research

Research – Inhibition of Listeria monocytogenes by Phage Lytic Enzymes Displayed on Tailored Bionanoparticles

Posted on March 31, 2022 by | Leave a comment

MDPI

The high mortality rate associated with Listeria monocytogenes and its ability to adapt to the harsh conditions employed in food processing has ensured that this pathogen remains a serious problem in the ready-to-eat food sector. Bacteriophage-derived enzymes can be applied as biocontrol agents to target specific foodborne pathogens. We investigated the ability of a listeriophage endolysin and derivatives thereof, fused to polyhydroxyalkanoate bionanoparticles (PHA_BNPs), to lyse and inhibit the growth of L. monocytogenes. Turbidity reduction assays confirmed the lysis of L. monocytogenes cells at 37 °C upon addition of the tailored BNPs. The application of BNPs also resulted in the growth inhibition of L. monocytogenes. BNPs displaying only the amidase domain of the phage endolysin were more effective at inhibiting growth under laboratory conditions (37 °C, 3 × 107 CFU/mL) than BNPs displaying the full-length endolysin (89% vs. 83% inhibition). Under conditions that better represent those found in food processing environments (22 °C, 1 × 103 CFU/mL), BNPs displaying the full-length endolysin demonstrated a greater inhibitory effect compared to BNPs displaying only the amidase domain (61% vs. 54% inhibition). Our results demonstrate proof-of-concept that tailored BNPs displaying recombinant listeriophage enzymes are active inhibitors of L. monocytogenes. View Full-Text

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Posted in Bacteriophage, Decontamination Microbial, Food Micro Blog, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, Listeria, Listeria monocytogenes, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Research, Technology

Research – Enhanced antimicrobial effectiveness of synergistic mixtures of rambutan peel extract and cinnamon essential oil on food spoilage bacteria and bio-based food packaging

Posted on March 31, 2022 by | Leave a comment

Wiley Online

Staph

This research aims to enhance antimicrobial activity of rambutan peel extract (RPE), by mixing with cinnamon essential oil (CEO). The mixture of RPE and CEO with different weight ratios (10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, and 0:10) had been prepared and the antimicrobial efficacy was tested. Nine strains of bacteria: Gram-positive bacteria (Bacillus sp., Enterococcus faecalis, Leuconostoc sp., Micrococcus luteus, Staphylococcus aureus, and Streptococcus sp.) and Gram-negative bacteria (Escherichia coli, Pseudomonas fluorescens, and Salmonella typhimurium) were selected as the representative of pathogenic and food spoilage bacteria. Mixing RPE with CEO in a ratio of 5:5 showed the best synergistic effect against those bacteria. Addition of RPE/CEO in a weight ratio of 5:5 improved the antimicrobial activity of whey protein isolated (WPI) films, and enhanced strength, stiffness, and water resistance of the film.

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Posted in Bacillus, Decontamination Microbial, E.coli, Faecal Streptpcocci, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Pseudomonas fluorescens, Research, Salmonella, Staphylococcus aureus, Technology

Research – Impact of the COVID-19 pandemic on gastrointestinal infection trends in England, February–July 2020

Posted on March 31, 2022 by | Leave a comment

bmj

Abstract

Objective To establish the impact of the first 6 months of the COVID-19 outbreak response on gastrointestinal (GI) infection trends in England.

Design Retrospective ecological study using routinely collected national and regional surveillance data from seven UK Health Security Agency coordinated laboratory, outbreak and syndromic surveillance systems using key dates of UK governmental policy change to assign phases for comparison between 2020 and historic data.

Results Decreases in GI illness activity were observed across all surveillance indicators as COVID-19 cases began to peak. Compared with the 5-year average (2015–2019), during the first 6 months of the COVID-19 response, there was a 52% decrease in GI outbreaks reported (1544 vs 3208 (95% CI 2938 to 3478)) and a 34% decrease in laboratory confirmed cases (27 859 vs 42 495 (95% CI 40 068 to 44 922)). GI indicators began to rise during the first lockdown and lockdown easing, although all remained substantially lower than historic figures. Reductions in laboratory confirmed cases were observed across all age groups and both sexes, with geographical heterogeneity observed in diagnosis trends. Health seeking behaviour changed substantially, with attendances decreasing prior to lockdown across all indicators.

Conclusions There has been a marked change in trends of GI infections in the context of the COVID-19 pandemic. The drivers of this change are likely to be multifactorial; while changes in health seeking behaviour, pressure on diagnostic services and surveillance system ascertainment have undoubtably played a role, there has likely been a true decrease in the incidence for some pathogens resulting from the control measures and restrictions implemented. This suggests that if some of these changes in behaviour such as improved hand hygiene were maintained, then we could potentially see sustained reductions in the burden of GI illness.

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Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, gastroenteritis, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Research

Research – Prevalence, serovar distribution, and antibiotic resistance of Salmonella spp. isolated from pork in China: A systematic review and meta-analysis

Posted on March 31, 2022 by | Leave a comment

Science Direct

120px-Sow_with_piglet

Highlights

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A meta-analysis of epidemiological characteristics of Salmonella isolated from pork in China was performed.

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Explored the source of heterogeneity by subgroup analysis and meta-regression.

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Confirmed an alarming prevalence of Salmonella in pork, and an increasing trend over time.

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Derby, Typhimurium, and London dominate most regions; high antibiotic resistance aggravates public health burden.

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Improvement of the prevention and control strategies of Salmonella in pork is necessary.

Abstract

The epidemiological characteristics of Salmonella spp. in pork have been widely studied in China, but the results remain inconsistent. This study aimed to summarize the epidemiological characteristics of Salmonella spp. isolated from pork, including its prevalence, serovar distribution, and antibiotic resistance rate. We systematically reviewed published studies on Salmonella spp. isolated from pork in China between 2000 and 2020 in two Chinese and three English databases and quantitatively summarized its prevalence, serovar distribution, and antibiotic resistance using meta-analysis methods. Furthermore, we conducted subgroup analysis and meta-regression to explore the source of the heterogeneity from historical changes and regional difference perspectives. Ninety-one eligible studies published between 2000 and 2020 were included. The meta-analysis showed that the pooled prevalence of Salmonella isolated from pork was 0.17 (95% CI: 0.14, 0.20), with a detected growing trend over time. For the proportions of serovars, Derby (0.32, 95% CI: 0.26, 0.38), Typhimurium (0.10, 95% CI: 0.07, 0.15) and London (0.05, 95% CI: 0.03, 0.08) were dominant in these studies. The antibiotic resistance rates were high for tetracycline (0.68, 95% CI: 0.59, 0.77), sulfisoxazole (0.65, 95% CI: 0.45, 0.83), ampicillin (0.43, 95% CI: 0.34, 0.53), streptomycin (0.42, 95% CI: 0.29, 0.56), and sulfamethoxazole (0.42, 95% CI: 0.25, 0.60). The results of this study revealed a high prevalence, the regional characteristics of serovar distribution, and the severe challenges of antibiotic resistance of Salmonella originating from pork in China, suggesting the potential increasing risk and disease burden. Therefore, it is necessary to improve the prevention and control strategies of Salmonella in pork.

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Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Research, Salmonella

USA – A Decade of E. coli Outbreaks in Leafy Greens in the U.S. and Canada – Podcast

Posted on March 31, 2022 by | Leave a comment

CDC

Katherine Marshall, an epidemiologist at CDC, and Sarah Gregory discuss E. coli outbreaks in leafy greens in the United States and Canada from 2009 to 2018.

More info on this topic
Topics: E. coli Infection, Foodborne Disease, Podcasts
Published: 9/24/2020
Last Updated: 9/24/2020

This podcast belongs to the Emerging Infectious Diseases series.

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Posted in E.coli, food bourne outbreak, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, foodborne outbreak, foodbourne outbreak, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, outbreak, Research, STEC, STEC E.coli

Research – Zoonotic Pathogens in Wildlife Traded in Markets for Human Consumption, Laos

Posted on March 30, 2022 by | Leave a comment

CDC

Abstract

We tested animals from wildlife trade sites in Laos for the presence of zoonotic pathogens. Leptospira spp. were the most frequently detected infectious agents, found in 20.1% of animals. Rickettsia typhi and R. felis were also detected. These findings suggest a substantial risk for exposure through handling and consumption of wild animal meat.

Consumption of wildlife meat drives emerging infectious diseases , often amplified by human encroachment into natural areas and changes in land use. Wildlife trade and consumption have been responsible for outbreaks of diseases such as HIV-1 , Ebola , and monkeypox and possibly for the coronavirus disease pandemic . Wildlife markets bring diverse species into contact, usually in dense and unsanitary conditions, enabling mixing, amplification, and transmission of pathogens among species, including humans . Small mammals host diverse pathogenic bacteria and viruses , but little investigation of endemic bacteria transmission has occurred. Determining pathogens present in traded wildlife is vital to guide appropriate measures to combat zoonotic diseases and document societal and environmental costs of wildlife trade.

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Posted in Bacteria, bacterial contamination, Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Pathogen, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Pathogen, pathogenic, Research, Virus