Category Archives: Microbiology

Research – Microbiological contamination of ready to eat algae and evaluation of Bacillus cereus behavior by microbiological challenge test

Journal of Food Protection

Consumption of seaweeds, often categorized as a superfood, is becoming popular in western countries. Algae can be marketed fresh, but are usually sold dehydrated to ensure longer shelf life. The consumption, often as ready to eat, open up possible risks for public health because of foodborne pathogens that can contaminate the raw material during harvesting or manipulation. In this study, fourteen ready to eat foods based on dehydrated algae, representative of the most consumed species, were considered. The microbial content, with a focus on Listeria monocytogenes and Bacillus cereus, was investigated by plate counts and B. cereus strains were isolated and identified by 16s rRNA gene sequencing. The microbiological quality was heterogeneous among the samples and, in particular, marine bacteria, Listeria spp., B. cereus and coliforms were detected. To contribute to related risk assessment, the ability of B. cereus to grow during refrigerated storage was evaluated, to our knowledge for the first time, by a microbiological challenge test on two ready to eat foods based on Undaria pinnatifida and Palmaria palmata.  Despite this study demonstrating the inability of B. cereus to proliferate in seaweed-based food, its presence in dehydrated foodstuffs cannot rule out the replication after rehydration before consumption, making it necessary to shed light on the possible risks for consumers.

Research – Biofilm-forming ability of poultry Campylobacter jejuni strains in the presence and absence of Pseudomonas aeruginosa

Canadian Journal of Microbiology

The aims of this study were to evaluate the ability of Campylobacter jejuni isolated from a poultry slaughterhouse to form biofilm in the presence and absence of Pseudomonas aeruginosa, and the effect of surface (stainless steel, polystyrene), temperature (7, 25, and 42 °C), and oxygen concentration (microaerophilic and aerobic conditions) on the formation of biofilm. The genes ahpCcadFclpPdnaJdocAflaAflaBkatAkpsMluxSracR, and sodB, related to biofilm formation by C. jejuni, were also investigated. All isolates formed biofilm on stainless steel and on polystyrene, in both aerobic and microaerophilic atmospheres, including temperatures not optimal for C. jejuni growth (7 and 25 °C), and biofilm also was formed in the presence of P. aeruginosa. In dual-species biofilm on stainless steel, biofilm formation was 2–6 log CFU·cm−2 higher at 7 °C for all isolates, in comparison with monospecies biofilm. Ten genes (ahpCcadFclpPdnaJdocAflaAflaBluxSracR, and sodB) were detected in all isolates, but katA and kpsM were found in four and six isolates, respectively. The results obtained are of concern because the poultry C. jejuni isolates form biofilm in different conditions, which is enhanced in the presence of other biofilm formers, such as P. aeruginosa.

Research – Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups

Springer

Shiga toxin-producing Escherichia coli (STEC) are foodborne bacterial pathogens, with cattle a significant reservoir for human infection. This study evaluated environmental reservoirs, intermediate hosts and key pathways that could drive the presence of Top 7 STEC (O157:H7, O26, O45, O103, O111, O121 and O145) on pasture-based dairy herds, using molecular and culture-based methods. A total of 235 composite environmental samples (including soil, bedding, pasture, stock drinking water, bird droppings and flies and faecal samples of dairy animals) were collected from two dairy farms, with four sampling events on each farm. Molecular detection revealed O26, O45, O103 and O121 as the most common O-serogroups, with the greatest occurrence in dairy animal faeces (> 91%), environments freshly contaminated with faeces (> 73%) and birds and flies (> 71%). STEC (79 isolates) were a minor population within the target O-serogroups in all sample types but were widespread in the farm environment in the summer samplings. Phylogenetic analysis of whole genome sequence data targeting single nucleotide polymorphisms revealed the presence of several clonal strains on a farm; a single STEC clonal strain could be found in several sample types concurrently, indicating the existence of more than one possible route for transmission to dairy animals and a high rate of transmission of STEC between dairy animals and wildlife. Overall, the findings improved the understanding of the ecology of the Top 7 STEC in open farm environments, which is required to develop on-farm intervention strategies controlling these zoonoses.

Belgium – Goat Cheese with Salmon Recall – Listeria monocytogenes

AFSCA

Recall of the Belle Chèvre company
Product: Goat cheese with salmon.
Problem: Possible presence of Listeria Monocytogenes.


In agreement with the AFSCA, Belle Chèvre is withdrawing from sale the goat cheese with salmon “Ambachtelijke geitenkaas met gerookte zalm” (4x75g) and is recalling it to consumers because of the possible presence of Listeria Monocytogenes.

Belle Chèvre asks its customers not to consume this product and to bring it back to the point of sale where it was purchased where they will be reimbursed.

Product Description: Product

Name: Ambachtelijke geitenkaas met gerookte zalm (4x75g)
Brand: Belle Chèvre
Minimum Durability Date (DDM): Multiple dates affected. See label with “date + bij max. + 7 ° C ”applied by the point of sale
Lot number: 0803211202
Sales period: from 3/12/2020 to 03/17/2021
Type of packaging: Transparent plastic packaging and under vacuum
Weight: Per 4 pieces of 75 g.

Product photo:

   

The product was sold by: Baliehof (Luc Callemeyn) Gistelsteenweg, 577 – 8490 Jabbeke.

For any further information , contact:

Sophie Haerinck via mail: sophie.haerinck@gmail.com
GSM: 0474 / 51.09.50

France – Product recall: Monts de Joux brand Mini Mont d’Or – Listeria monocytogenes

Oulah

Product recall: Monts de Joux brand Mini Mont d'Or

ENCOUNTERED PROBLEM

Presence of Listeria monocytogenes

PROPOSED SOLUTION

People who may have this product are asked not to consume it and to return it to the point of sale where it was purchased.

People who have consumed this product and who present with fever, isolated or accompanied by headaches, are invited to consult their attending physician, notifying him of this consumption.
Pregnant women should pay special attention to these symptoms, as well as immunocompromised people and the elderly. These symptoms may suggest listeriosis, a disease that can be serious and can take up to eight weeks to incubate.

FURTHER INFORMATION

▸ Barcode
3476810000702

▸ Lot
3053

▸ DLC
04/06/2021

▸ Marketing
from 03/03 to 18/03/2021

▸ Health
stamp FR 25.041.001 CE

▸ Consumer service contact
06.07.24.50.16

▸ Source
https://www.auchan.fr/

RASFF Alerts – STEC E.coli – Soft Cheese – Chilled Boneless Beef

European Food Alerts

RASFF

shigatoxin-producing Escherichia coli (stx2+ /25g) in soft cheese from France in Germany

RASFF

shigatoxin-producing Escherichia coli (O185:H7, stx2+ /25g) in chilled boneless beef from Argentina in Germany

RASFF Alerts – Listeria monocytogenes – Smoked Trout Fillet – Raw Milk Cheese – Soft Cheese

European Food Alerts

RASFF

Listeria monocytogenes (>15000 CFU/g) in smoked trout fillet from Germany in the Netherlands

RASFF

Listeria monocytogenes (detected /25g) in raw milk cheese from Belgium in Germany

RASFF

Listeria monocytogenes (presence /25g) in soft cheese from Austria in Austria

RASFF Alerts – Salmonella – Chilled Turkey Breast Fillets – Frozen Hard Clams -Liquid Chocolate – Chilled Pork – Chilled Broiler Fillets – Chicken Breast – Chicken Drumsticks – Duck Meat

European Food Alerts

RASFF

Salmonella enterica ser. Typhimurium (presence /25g) in chilled turkey breast fillets from Poland in Lithuania

RASFF

Salmonella (presence /25g) in frozen hard clams (Meretrix lyrata) from Vietnam in Italy

RASFF

Salmonella enterica ser. Agona (presence /250g) in liquid chocolate from the Netherlands in the Netherlands

RASFF

Salmonella enterica ser. Typhimurium (detected /25g) in chilled pork from Germany in Norway

RASFF

Salmonella enterica ser. Enteritidis (presence /25g) and Salmonella enterica ser. Infantis (presence /25g) in chilled broiler fillets from Poland in Latvia

RASFF

Salmonella (presence /25g) in chilled chicken breasts reared in Germany and slaughtered in the Netherlands in Ireland

RASFF

Salmonella enterica ser. Typhimurium (in 2 out of 5 samples /25g) in chilled chicken drumsticks from Poland in Poland

RASFF

Salmonella enterica ser. Enteritidis (presence /25g) in frozen duck meat from Poland, via the Netherlands in Finland

RASFF Alerts – Animal Feed – Salmonella – Fish Meal – Rapeseed Meal – Frozen Raw Pet Foods

European Food Alerts

RASFF

Salmonella (in 2 out of 5 samples /25g) in fish meal from Peru, via France in Belgium

RASFF

Salmonella enterica ser. Fillmore (presence /25g) in rapeseed meal from Romania in Romania

RASFF

Salmonella enterica ser. Typhimurium (presence /25g) in frozen raw petfood from the Netherlands in Belgium

USA – USDA -FSIS Outbreak Table

FSIS USDA

The table below lists outbreak investigations in which FSIS responded by posting a recall of FSIS-regulated product linked to illness, a public health alert, and/or an after-action review report. The table does not include outbreak investigations that did not result in one or more of these outcomes.

The Outcomes & References column includes links to recall notices, public health alerts, and after-action review reports, as well as CDC outbreak notices for selected multistate foodborne outbreaks, which provide additional information. For a list of all foodborne outbreaks reported to CDC since 1998, please use the CDC National Outbreak Reporting System Dashboard.

Outbreak Year Pathogen Product Outcomes & References Active
2021 Salmonella Hadar Turkey (suspect) Yes
2021 Escherichia coli O145 (E-coli) Beef, ground (suspect) No
2021 Escherichia coli O157:H7 (E-coli) Unknown

CDC Notice: View CDC Investigation Notice

No
2021 Listeria monocytogenes Unknown No
2021 Escherichia coli O157:H7 (E-coli) Beef, ground (suspect) Yes
2020 Listeria monocytogenes Italian-style deli meats (suspect)

CDC Notice: View CDC Investigation Notice

No
2019 Salmonella Schwarzengrund Turkey, ground FSIS Recall: Butterball LLC Recalls Turkey Products Due to Possible Salmonella Schwarzengrund Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Schwarzengrund-03-2019

No
2019 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: New Seasons Market Recalls Ready-To-Eat Sesame Noodle Chicken Salad Products Due to Misbranding and Undeclared Allergens
FSIS After-Action Review:

Yes
2019 Salmonella Beef, ground FSIS Recall: Central Valley Meat Co., Inc. Recalls Ground Beef Products Due to Possible Salmonella Dublin Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Dublin-11-2019

No
2019 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: K2D Foods Recalls Raw Ground Beef Products Due to Possible E. coli O103 Contamination

CDC Notice: CDC Notice: O103-04-2019

No
2018 Salmonella Typhimurium Chicken salad FSIS Recall: Triple T Specialty Meats Inc. Recalls Chicken Salad Products Due to Possible Salmonella Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Typhimurium-02-2018

No
2018 Listeria monocytogenes Pork patty rolls FSIS Recall: 165368 C. Corporation Recalls Pork Products Due to Possible Listeria Contamination

CDC Notice: CDC Notice: Lm-11-2018

No
2018 Salmonella Reading Turkey, ground FSIS Recall: Jennie-O Turkey Store Sales, Inc. Recalls Raw Ground Turkey Products due to Possible Salmonella Reading Contamination
FSIS Recall: Jennie-O Turkey Store Sales, LLC Recalls Raw Ground Turkey Products due to Possible Salmonella Reading Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Reading-07-2018

No
2018 Salmonella Newport Beef, ground FSIS Recall: JBS Tolleson, Inc. Recalls Raw Beef Products due to Possible Salmonella Newport Contamination
FSIS Recall: JBS Tolleson, Inc. Recalls Raw Beef Products Due to Possible Salmonella Newport Contamination

CDC Notice: CDC Notice: Newport-10-2018

No
2018 Listeria monocytogenes Ham, ready-to-eat FSIS Recall: Johnston County Hams Recalls Ready-To-Eat Ham Products Due to Possible Listeria Contamination
FSIS Recall: Ukrop’s Homestyle Foods Recalls Ready-To-Eat Meat and Poultry Products Due to Possible Listeria Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Lm-10-2018

No
2018 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: Publix Super Markets Inc. Recalls Ground Chuck Products Due To Possible E. coli O26 Contamination
FSIS Recall: Cargill Meat Solutions Recalls Ground Beef Products due to Possible E. coli O157:H7 Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: O26-09-2018

No
2018 Salmonella I 4,[5],12:i:- Chicken FSIS Recall: Empire Kosher Poultry Inc. Recalls Chicken Products Due To Possible Extraneous Material Contamination

CDC Notice: CDC Notice: Salmonella-08-2018

No
2018 Salmonella Enteritidis Chicken, raw, breaded FSIS After-Action Review:

No
2017 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: Vermont Livestock Slaughter And Processing LLC, Recalls Ground Beef Due To Possible E. Coli O157:H7 Contamination No
2016 Salmonella I 4,[5],12:i:- Chicken, rotisserie FSIS After-Action Review:

No
2016 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: Adams Farm Slaughterhouse Recalls Beef, Veal, And Bison Products Due To Possible E. Coli O157:H7 Adulteration

CDC Notice: CDC Notice: O157H7-09-2016

No
2016 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: PT Farm, LLC Recalls Beef Products Due To Possible E. Coli O157:H7 Contamination No
2016 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: Good Food Concepts, LLC D.B.A. Ranch Foods Direct Recalls Non-Intact Beef Products Due To Possible E. Coli O157:H7 Contamination No
2016 Salmonella I 4,[5],12:i:- Pork (including at pig roasts) FSIS Recall: Kapowsin Meats Inc. Recalls Pork Products Due To Possible Salmonella Contamination
FSIS After-Action Review:

No
2016 Escherichia coli O157:H7 (E-coli) Beef, ground FSIS Recall: Snapp’s Ferry Packing Company Recalls Beef Product Due To Possible E. Coli O157:H7 Contamination No
2015 Salmonella I 4,[5],12:i:- and Salmonella Infantis Pork (including at pig roasts) FSIS Recall: Kapowsin Meats Recalls Pork Product Due To Possible Salmonella Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Salmonella-08-2015

No
2015 Salmonella Enteritidis Chicken, stuffed FSIS Recall: Aspen Foods Recalls Frozen, Raw, Stuffed & Breaded Chicken Products Due to Possible Salmonella Enteritidis Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Enteritidis-07-2015

No
2015 Salmonella Enteritidis Chicken, stuffed FSIS Recall: Barber Foods Recalls Stuffed Chicken Products Due To Possible Salmonella Enteritidis Contamination
FSIS After-Action Review:

CDC Notice: CDC Notice: Enteritidis-07-2015

No