Category Archives: E.coli O26

Research – Evaluation of Bactericidal Effects of Phenyllactic Acid on Escherichia coli O157:H7 and Salmonella Typhimurium on Beef Meat

Journal of Food Protection


Bactericidal effects of various concentrations of phenyllactic acid on Shiga toxin–producing Escherichia coli (STEC), including E. coli O157:H7, O26:H11, O103:H2, and O121:H19, and on Salmonella Typhimurium DT104 in pure culture and microplates assays were studied. Beef cuts were surface sprayed with phenyllactic acid or lactic acid for inactivation of E. coli O157:H7 and Salmonella Typhimurium. The 1.5% phenyllactic acid inactivated all inoculated E. coli O157:H7, O26:H11, O103:H2, and O121:H19 and Salmonella Typhimurium DT104 (>6-log reduction) within 1 min of contact at 21°C, whereas 1.5% lactic acid did not result in microbial reduction. Microplate assays (for STEC and Salmonella Typhimurium DT104 at 10 to 100 CFU per well) indicated that concentrations of 0.25% phenyllactic acid or 0.25% lactic acid inhibited the growth of STEC and Salmonella Typhimurium DT104 incubated at 37°C for 24 h. Treatment of beef with 1.5% lactic acid or 1.5% phenyllactic acid reduced E. coli O157:H7 by 0.22 and 0.38 log CFU/cm2, respectively, within 5 min and reduced Salmonella Typhimurium DT104 by 0.12 and 0.86 log CFU/cm2, respectively. When meat treated with 1.5% phenyllactic acid was frozen at −20°C, inactivation of E. coli O157 and Salmonella Typhimurium DT104 was enhanced by 1.06 and 1.46 log CFU/cm2, respectively. Thus, treatment of beef with 1.5% phenyllactic acid significantly reduced the population of E. coli O157:H7 and Salmonella.

  • Phenyllactic acid at 1.5% killed STEC and Salmonella (>6-log reduction) within 1 min.

  • The MIC of lactic and phenyllactic acids was 0.25%.

  • The bactericidal effect of phenyllactic acid on beef was enhanced by freezing.

USA -King Arthur Flour Updates Three Lot Codes of Voluntarily Recalled Unbleached All-Purpose Flour (5 lb.)


King Arthur Flour, Inc. was notified by ADM Milling Co. that three additional product lot codes of Unbleached All-Purpose Flour 5 lb. were omitted from the original data they provided for the press release on October 3, 2019. The additional lot codes and their corresponding “Best Used By” dates are listed below:

Best Used by Date 12/09/19: Lot codes L18A09A L18A09C

Best Used by Date 01/08/20: Lot code A19A08A

This new information only applies to “Best Used By” dates already disclosed in the prior release. No additional Best Used By dates are introduced as a result of these three updated lot codes.

As stated in the prior release, we have undertaken this voluntary recall because of the potential presence of E. coli 026.

King Arthur Flour has not received any confirmed reports of illnesses to date related to this product.

Consumers who have any of these affected products should throw them away and may submit a claim for a refund or replacement at Link Disclaimer, or by calling our King Arthur Flour Consumer Hotline at 866-797-9178.

Consumer safety is our top priority. Consumers are reminded to wash their hands, work surfaces, and utensils thoroughly after contact with raw dough products or flour, and to never eat raw dough or batter. The Centers for Disease Control (CDC) warns consumers to not consume raw products made with flour. E. coli O26 is killed by heat through baking, frying, sautéing, or boiling products made with flour. For more information, refer to the following:

Bakers have trusted King Arthur Flour products in their kitchens for over 225 years. We remain committed to providing our consumers safe and superior products.

This information can be found online at Link Disclaimer Consumers with any questions regarding this recall or King Arthur Flour products are encouraged to call the King Arthur Flour Consumer Hotline at 866-797-9178.

Original Press Release

Research – Survey of Intact and Nonintact Raw Pork Collected at Retail Stores in the Mid-Atlantic Region of the United States for the Seven Regulated Serogroups of Shiga Toxin–Producing Escherichia coli

Journal of Food Protection


A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga toxin–producing Escherichia coli (STEC-7) using standard microbiological and molecular methods. In short, 21 (5.3%) of the 395 ground or nonintact pork samples and 3 (3.4%) of the 119 intact pork samples tested positive via the BAX system real-time PCR assay for the stx and eae virulence genes and for the somatic O antigens for at least one of the STEC-7 serogroups. However, none of these 24 presumptive-positive pork samples subsequently yielded a viable isolate of STEC displaying a STEC-7 serogroup-specific surface antigen in combination with the stx and eae genes. These data suggest that cells of STEC serogroups O26, O45, O103, O111, O121, O145, or O157:H7 are not common in retail raw pork samples in the mid-Atlantic region of the United States.

  • None of the 514 retail raw pork samples were positive for STEC-7.

  • Four of 514 raw pork samples harbored E. coli of unknown serogroup containing stx and eae.

  • STEC-7 are uncommon in retail raw pork samples in the U.S. mid-Atlantic region.


Research – Antibiofilm Efficacy of Peptide 1018 against Listeria monocytogenes and Shiga Toxigenic Escherichia coli on Equipment Surfaces

Journal of Food Protection


Listeria monocytogenes and Shiga toxigenic Escherichia coli (STEC) are important foodborne bacterial pathogens that can form biofilms on equipment surfaces at food processing facilities. Pathogens in biofilms are resistant to conventional antimicrobials and require higher antimicrobial concentrations to be inactivated. In this study, the efficacy of a synthetic innate defense regulator peptide 1018 (peptide 1018) for inactivating L. monocytogenes and STEC (O26, O111, O145, O157) biofilms on stainless steel and polycarbonate surfaces was investigated. Stainless steel and polycarbonate coupons (12 mm in diameter) were used in a Centers for Disease Control and Prevention biofilm reactor containing 400 mL of 10% tryptic soy broth (TSB) that had been inoculated with an individual strain of L. monocytogenes or STEC to obtain 6 log CFU/mL populations. The reactor was set with a constant flow rate at 50 mL/h of 10% TSB for 48 h. After 48 h, coupons were treated with peptide 1018 at 0, 10, 20, or 50 μg/mL in phosphate buffer saline (PBS) for 24 h. Surviving bacterial populations were determined by scraping off the coupons and spiral plating on selective media. Significantly higher levels of pathogens in biofilms formed by certain bacterial strains, including L. monocytogenes F6854, E. coli O157:H7 RM4407 and NADC5713, and non-O157 E. coli NADC3629, were recovered on polycarbonate surfaces than on stainless steel. Antibiofilm efficacy of peptide 1018 against pathogens was concentration-dependent and varied with the type of pathogen and material surfaces. Peptide 1018 at 50 μg/mL significantly inactivated all tested bacterial biofilms on both surfaces compared with the PBS control (P < 0.05). L. monocytogenes was the bacterium most sensitive to peptide 1018; on stainless steel surfaces treated with 50 μg/mL peptide 1018, there was a 3.7- to 4.6-log CFU/cm2 reduction in Listeria populations compared with a 1.0- to 3.5-log CFU/cm2 reduction of STEC. Results suggest that peptide 1018 may be used to inactivate L. monocytogenes and STEC biofilms on equipment surfaces.

  • Bacteria attach at higher levels on polycarbonate surfaces than on stainless steel.

  • L. monocytogenes is more sensitive than STEC to peptide 1018.

  • Peptide 1018 can be used to inactivate biofilms on equipment surfaces.

USA -The J. M. Smucker Company Issues Voluntary Recall of Specific Lots of Robin Hood® All Purpose Flour Distributed and Sold in the U.S. Only


Company Announcement

Out of an abundance of caution, The J. M. Smucker Company today announced a voluntary recall of specific lots of Robin Hood® All Purpose Flour distributed and sold in the U.S. due to possible E. coli contamination. This recall does not impact any Robin Hood® items sold in Canada.

No other items manufactured by The J. M. Smucker Company, including other Robin Hood® products distributed and sold in the U.S. or Canada, are impacted by this issue. No illnesses related to this issue have been reported to date.

The impacted products are as follows:

Product Name

UPC Code – Case

UPC Code – Item

Lot Codes

Best if Used By Dates

Robin Hood® All Purpose Flour (5 lb) 0 51500 18010 2 0 51500 18010 5 8350 513
8351 513
8354 513
8355 513

These products were distributed by The J. M. Smucker Company to a variety of retailers in the U.S.

Consumers who have impacted product should stop using the products and should dispose of them. If consumers have questions or have products covered by this recall, they should email the Company by completing this formExternal Link Disclaimer or calling 888-569-6728, Monday through Friday, between 8:00 a.m. and 5:00 p.m. ET.

The recall is being conducted in cooperation with the U.S. Food and Drug Administration.

Europe -E. coli and Listeria concerns prompt cheese recall in five countries

Food Safety News

Raw cow’s milk cheese distributed to at least five European countries has been recalled due to possible E. coli and Listeria contamination.

A warning was issued by German authorities about E. coli O26:H11 and Listeria monocytogenes in the raw cow’s milk Bethmale cheese from France. There have been no illnesses linked to the recall.

Affected cheese has also been distributed to Austria, Belgium, Spain and Switzerland, according to the Rapid Alert System for Food and Feed (RASFF).

Recalled Moulis cremier cheese

The French company Le Moulis SAS recalled products with the names “Moulis vache, Moulis vache prestige” and “Bethmale de marterat” due to Listeria. The items have lot numbers 19163112, 19170119, 19177126, 19184103, 19191110, 19199118 and 19205124 plus best before dates Nov. 29, Dec. 1, 5, 8, 13, 20 and 22, 2019. They have the product code FR 09.214.001.CE.

The firm, based in Luzenac, Moulis in France, also recalled “Moulis vache and Moulis vache prestige” with batch number 19228116 and dates Dec. 20 and 27, 2019 due to Shiga toxin-producing E. coli (STEC) concerns. The items can be identified by the number FR 09.214.001 CE on the pack.

Spanish authority’s warning

The Spanish Agency for Food Safety and Nutrition (AESAN) issued an alert on certain batches of the raw cow’s milk cheese.

Recalled Le Moulis cheese

Distribution in Spain includes “Moulis cremier” with lot number 19163112 and expiry date Dec. 1, 2019; “Moulis Vache” with lot number 19199118 and expiry date Dec 5, 2019 and “Bethmale de Marterat Vache” with lot number 19199118 and expiry date Dec. 20, 2019.

Singapore – King Arthur Flour Unbleached All-Purpose Flour due to the potential presence of E. coli O26


Recall of King Arthur Flour Unbleached All-Purpose Flour
The United States of America Food and Drug Administration (USFDA) has
published an alert on the recall of King Arthur Flour Unbleached All-Purpose Flour due
to the potential presence of E. coli O26.
2 As there is import of the implicated product into Singapore, the Singapore Food
Agency (SFA) has directed the sole importer, Cold Storage, to recall the product. The
recall is completed.
3 Consumers who have purchased the affected product should not consume it.
They may contact Cold Storage at 1800 8918 100 for enquiries or exchange of the