Category Archives: E.coli O103

Research – Evaluation of Bactericidal Effects of Phenyllactic Acid on Escherichia coli O157:H7 and Salmonella Typhimurium on Beef Meat

Journal of Food Protection

ABSTRACT

Bactericidal effects of various concentrations of phenyllactic acid on Shiga toxin–producing Escherichia coli (STEC), including E. coli O157:H7, O26:H11, O103:H2, and O121:H19, and on Salmonella Typhimurium DT104 in pure culture and microplates assays were studied. Beef cuts were surface sprayed with phenyllactic acid or lactic acid for inactivation of E. coli O157:H7 and Salmonella Typhimurium. The 1.5% phenyllactic acid inactivated all inoculated E. coli O157:H7, O26:H11, O103:H2, and O121:H19 and Salmonella Typhimurium DT104 (>6-log reduction) within 1 min of contact at 21°C, whereas 1.5% lactic acid did not result in microbial reduction. Microplate assays (for STEC and Salmonella Typhimurium DT104 at 10 to 100 CFU per well) indicated that concentrations of 0.25% phenyllactic acid or 0.25% lactic acid inhibited the growth of STEC and Salmonella Typhimurium DT104 incubated at 37°C for 24 h. Treatment of beef with 1.5% lactic acid or 1.5% phenyllactic acid reduced E. coli O157:H7 by 0.22 and 0.38 log CFU/cm2, respectively, within 5 min and reduced Salmonella Typhimurium DT104 by 0.12 and 0.86 log CFU/cm2, respectively. When meat treated with 1.5% phenyllactic acid was frozen at −20°C, inactivation of E. coli O157 and Salmonella Typhimurium DT104 was enhanced by 1.06 and 1.46 log CFU/cm2, respectively. Thus, treatment of beef with 1.5% phenyllactic acid significantly reduced the population of E. coli O157:H7 and Salmonella.

HIGHLIGHTS
  • Phenyllactic acid at 1.5% killed STEC and Salmonella (>6-log reduction) within 1 min.

  • The MIC of lactic and phenyllactic acids was 0.25%.

  • The bactericidal effect of phenyllactic acid on beef was enhanced by freezing.

USA – E. coli O103 illnesses in Tennessee prompt Ground Beef Recall

Food Poison Journal

K2D Foods, doing business as (DBA) Colorado Premium Foods, a Carrolton, Ga. establishment, is recalling approximately 113,424 pounds of raw ground beef products that may be contaminated with E. coli O103, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.

The raw ground beef items were produced on March 26, March 29, April 2, April 5, April 10, and April 12, 2019.  The following products are subject to recall:

  • Two 24-lb. vacuum-packed packages in cardboard boxes containing raw “GROUND BEEF PUCK” with “Use Thru” dates of 4/14/19, 4/17/19, 4/20/19, 4/23/19, 4/28/19, and 4/30/19.

The products subject to recall bear establishment number “EST. 51308” inside the USDA mark of inspection on the boxes. These items were shipped to distributors in Port Orange, Fla. and Norcross, Ga. for further distribution to restaurants.

Research – Survey of Intact and Nonintact Raw Pork Collected at Retail Stores in the Mid-Atlantic Region of the United States for the Seven Regulated Serogroups of Shiga Toxin–Producing Escherichia coli

Journal of Food Protection

ABSTRACT

A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga toxin–producing Escherichia coli (STEC-7) using standard microbiological and molecular methods. In short, 21 (5.3%) of the 395 ground or nonintact pork samples and 3 (3.4%) of the 119 intact pork samples tested positive via the BAX system real-time PCR assay for the stx and eae virulence genes and for the somatic O antigens for at least one of the STEC-7 serogroups. However, none of these 24 presumptive-positive pork samples subsequently yielded a viable isolate of STEC displaying a STEC-7 serogroup-specific surface antigen in combination with the stx and eae genes. These data suggest that cells of STEC serogroups O26, O45, O103, O111, O121, O145, or O157:H7 are not common in retail raw pork samples in the mid-Atlantic region of the United States.

HIGHLIGHTS
  • None of the 514 retail raw pork samples were positive for STEC-7.

  • Four of 514 raw pork samples harbored E. coli of unknown serogroup containing stx and eae.

  • STEC-7 are uncommon in retail raw pork samples in the U.S. mid-Atlantic region.

 

USA – Outbreak Investigation of E. coli Linked to Ground Bison from Northfork Bison Distributions, July 2019

FDA

September 13, 2019

The U.S. Food and Drug Administration (FDA), along with the Centers for Disease Control and Prevention (CDC), state and local partners in the U.S., and with the support of the Canadian Food Inspection Agency (CFIA), investigated a multistate outbreak of E. coli O121 and E. coli O103 illnesses likely linked to ground bison supplied by Northfork Bison Distributions Inc. of Saint-Leonard, Québec, Canada.

On September 13, 2019, CDC announced that the outbreak appears to be over.

During the investigation, FDA and CDC analyzed traceback and epidemiological information to determine that ground bison supplied by Northfork Bison Distributions Inc. was the likely cause of the illnesses. FDA laboratory analysis of bison patties packed by the firm on February 26th, 2019, confirmed the presence of one of the outbreak strains of E. coli.

Northfork Bison Distributions Inc. was forthcoming with information to aid in the investigation, and publicly announced a voluntary recall on July 16, 2019. This recall included ground bison meat and bison patties produced between February 22 and April 30, 2019.

FDA regulates bison meat because the authority is not assigned specifically to the United States Department of Agriculture’s (USDA) Food Safety Inspection Service (FSIS) in the Federal Meat Inspection Act (FMIA).

Recommendation

Because the product is frozen and may still be in freezers, distributors, retailers and restaurants should not distribute, use or serve ground bison (including bison burgers) recalled by Northfork Bison Distributions Inc.

Consumers should not eat products prepared using recalled ground bison (including bison burgers) sold under the Northfork Bison label including bison burgers sold to retailers in 4 x 4-ounce packages with expiration dates through October 8, 2020.

Buffalo Burger Canadian Bison Meat

Information – Two varieties of E. coli behind multiple U.S. outbreak infections – O103 and O121

Food Safety News

This is a link to a  list of outbreaks in recent years that were caused by E. coli O103 and E. coli O121.

Canada -Food Recall Warning – Ground bison products recalled due to E. coli O121 and O103

CFIA Natural Frontier Foods - Bison – ground meat

Recall details

Ottawa, July 16, 2019 – Northfork Bison Distributions Inc. is voluntarily recalling ground bison products from the marketplace due to possible E. coli O121 and O103 contamination. Consumers should not consume the recalled products see link above.

This recall was triggered by the company and a recall in another country. The CFIA is conducting a food safety investigation, which may lead to the recall of other products. If other high-risk products are recalled, the CFIA will notify the public through updated Food Recall Warnings.

The CFIA is verifying that industry is removing recalled product from the marketplace.

Illnesses

There have been no reported illnesses associated with the consumption of these products in Canada. However, there have been reported illnesses in the United States linked to these products.

 

USA – Outbreak Investigation of E. coli Linked to Ground Bison from Northfork Bison Distributions, July 2019 – E.coli O121 – E.coli O103

FDA

July 16, 2019

The U.S. Food and Drug Administration (FDA), along with the Centers for Disease Control and Prevention (CDC), state and local partners in the U.S., and with the support of the Canadian Food Inspection Agency (CFIA), are investigating a multistate outbreak of E. coli O121 and E. coli O103 illnesses likely linked to ground bison supplied by Northfork Bison Distributions Inc. of Saint-Leonard, Québec, Canada.

The FDA and CDC analyzed traceback and epidemiological information to determine that ground bison supplied by Northfork Bison Distributions Inc. is the likely cause of the illnesses.

FDA regulates bison meat because the authority is not assigned specifically to the United States Department of Agriculture’s (USDA) Food Safety and Inspection Service (FSIS) in the Federal Meat Inspection Act (FMIA).

Recommendation

Buffalo Burger Canadian Bison Meat

Distributors, retailers and restaurants should not distribute, use or serve ground bison (including bison burgers) recalled by Northfork Bison Distributions Inc.

As of July 16, 2019, Northfork Bison Distributions Inc. is voluntarily recalling its ground bison, referred to as Bison Ground, and its ground bison patties, referred to as Bison Burgers and/or Buffalo Burgers, produced between February 22, 2019, and April 30, 2019.

Consumers should not eat products prepared using recalled ground bison (including bison burgers) sold under the Northfork Bison label including Bison Burgers sold to retailers in 4 x 4-ounce packages with expiration dates through October 8, 2020.

Northfork Bison Distributions Inc. has been quick to initiate a voluntary recall and has been forthcoming with information to aid in the investigation. The investigation is ongoing and updates will be provided when available.