Category Archives: Research

Fresh vegetables are prone to foodborne pathogen infection due to improper cultivation methods and poor post-harvest handling practices. This paper describes the prevalence of foodborne pathogens in three vegetables (tomato, brinjal, and lablab) cultivated by small-scale producers of India. We assessed the possible entry of pathogens into the vegetable production chain. Samples from soil, manure, irrigation water, harvesting bins, and workers’ hands were assessed as sources of possible pathogen entry. In addition, further sampling was done to determine prevalence of these pathogens in the produce from the agricultural fields and markets. Total plate counts, yeast and mold count, total coliforms like general microbial indices, and possible foodborne pathogens like Salmonella, Escherichia coli O157, Staphylococcus aureus, Pseudomonas aeruginosa, and Norovirus were assessed in all the samples by standard microbiological, biochemical, and molecular methods. The results revealed that among the three vegetables we studied, brinjal had the highest load of general microbial indicators. Moreover, the total coliforms count of market vegetables was higher than that of on-farm vegetables, indicating poor hygiene in post-harvest operations. The pathogen Pseudomonas aeruginosa was found in the market tomatoes (0.35 log₁₀ cfu/g), and E. coli was detected in the market samples of brinjal (2.98–3.89 log₁₀ cfu/g) and lablab (0.59–2.21 log₁₀ cfu/g), thereby revealing fecal cross-contamination during pre or post-harvest operations. None of the vegetable samples were contaminated with E. coli O157:H7, Staphylococcus aureus, Salmonella sp., and norovirus. However, the microbiological source tracking of primary production farms showed that E. coli O157:H7 was present in manure (9/27), soil (6/27), harvest bins (6/27), and irrigation water samples (3/27) of SSP farms. In addition, soil (33%), manure (11%), and harvest bins (11%) had Pseudomonas aeruginosa. In conclusion, our study suggests that adequate knowledge of good agricultural practices for small-scale producers to cultivate microbiologically safe vegetable production and proper handling strategies for vegetable vendors is crucial to mitigate foodborne pathogen outbreaks through fresh vegetables.