Category Archives: Microbiology

Research -Risk assessment of enteric viruses along the food chain and in the population

EFSA

Abstract

Food-borne microbial illness contributes up to one third of global disease burden. The largest category of food-borne illness is gastroenteritis, the majority of which is caused by enteric viruses. Viruses like these are transmitted to food either by waste-contaminated waters, or by handling and transfer during processing.

An important tool for reducing or controlling food-borne microbial risk is risk analysis. This framework has been adopted globally to manage risks associated with microbial contamination in food. Several hundred microbial risk assessments (MRAs) have been published by different national and international organisations, for different food-hazard combinations. The use of MRAs in controlling and understanding virus risk has, to date, been limited, compared with the efforts made on bacterial pathogens. Given the large disease burden that viruses are responsible for, this disparity should be addressed. 

The main reasons for the relative lack of risk assessments are the difficulty in detecting and monitoring viruses compared with bacteria. This means less data on prevalence, concentration and inactivation, and allows viruses to remain silent contributors to global disease. There are also key conceptual differences between virus risk assessment and bacterial risk assessment. This project aimed to assess the current state of the art for food-borne virus risk assessment, then to progress the field further by using the data available to produce risk rankings and risk assessments.

This was done by a combination of literature reviewing and various risk assessment tools. The result was an assessment of the overall evidence base in the literature, a semi-quantitative ranking comparison between the viruses and foods of most concern, and a survey of inactivation methods, leading to a quantitative ranking of the effectiveness of each in reducing and managing food-borne virus risk.

Research – Identification of Genetic Markers for the Detection of Bacillus thuringiensis Strains of Interest for Food Safety

MDPI

Abstract

Bacillus thuringiensis (Bt), belonging to the Bacillus cereus (Bc) group, is commonly used as a biopesticide worldwide due to its ability to produce insecticidal crystals during sporulation. The use of Bt, especially subspecies aizawai and kurstaki, to control pests such as Lepidoptera, generally involves spraying mixtures containing spores and crystals on crops intended for human consumption. Recent studies have suggested that the consumption of commercial Bt strains may be responsible for foodborne outbreaks (FBOs). However, its genetic proximity to Bc strains has hindered the development of routine tests to discriminate Bt from other Bc, especially Bacillus cereus sensu stricto (Bc ss), well known for its involvement in FBOs. Here, to develop tools for the detection and the discrimination of Bt in food, we carried out a genome-wide association study (GWAS) on 286 complete genomes of Bc group strains to identify and validate in silico new molecular markers specific to different Bt subtypes. The analyses led to the determination and the in silico validation of 128 molecular markers specific to Bt, its subspecies aizawaikurstaki and four previously described proximity clusters associated with these subspecies. We developed a command line tool based on a 14-marker workflow, to carry out a computational search for Bt-related markers from a putative Bc genome, thereby facilitating the detection of Bt of interest for food safety, especially in the context of FBOs.

Australia – Cause of illnesses from spinach in Australia identified; Costco among stores that received product

Food Safety News

Investigations into almost 200 foodborne illnesses in Australia after eating a brand of baby spinach have revealed what caused the illnesses.

Riviera Farms said the spinach was contaminated with a weed called thornapple. The scientific name is Datura stramonium and it is also known as jimsonweed. How the weed got into the food supply is still being investigated by Victorian authorities with site inspections underway.

Riviera Farms issued a recall of baby spinach after reports of customers falling ill. The company then contacted its 20 clients. Costco is the only direct major retail client, however, the product was also sold to stores such as Coles, Aldi, and Woolworths.

Spinach products were grown on a farm in Victoria and shipped to several stores across the country. More than 190 potential cases were reported in New South Wales, Australian Capital Territory, Victoria, and Queensland.

There have been several hospitalizations. Toxicological impacts are still to be confirmed but it is understood most people experienced symptoms for a short time and then recovered.

RASFF Alert – Animal Feed – Aflatoxin – Corn Gluten

RASFF

Aflatoxin B1 in corn gluten from Austria in Germany and Switzerland

RASFF Alerts – Animal Feed – Salmonella

RASFF

Salmonella Enteritidis in potato starch for feed from Belgium in the Netherlands

RASFF

Salmonella in complete feed for dogs from Netherlands in Belgium

RASFF

Salmonella in fishmeal from Spain in Belgium

The Handwashing Handbook

Global Handwashing Org

The Handwashing Handbook is based on the experience of the Global Handwashing Partnership and presents best practices and new concepts to improve the uptake of handwashing. The Handwashing Handbook focuses on:

  • Making the Case for Handwashing
  • Designing and Implementing Handwashing Programs
  • Improving Handwashing in Specific Contexts
  • Addressing Handwashing at a Systems Level

The Handwashing Handbook is available in Arabic, Chinese, English, French, Portuguese, and Spanish.

Resource Attachments:
https://globalhandwashing.org/wp-content/uploads/2020/10/GHP_Handwashing-Handbook_FINAL.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Arabic.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Chinese.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-French.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Portuguese.pdf (pdf)
https://globalhandwashing.org/wp-content/uploads/2020/10/Handwashing-Handbook-Spanish.pdf (pdf)

BFR

Flour is a natural product and a valuable foodstuff.

However, Shiga toxin-producing Escherichia coli (STEC) were detected in multiple flour samples (wheat, spelt and rye) during routine food monitoring in Germany in 2018. Escherichia (E.) coli are bacteria that occur naturally in the intestines of animals and humans and the detection of E. coli in food is a strong indicator of a faecal contamination.

Bacteria from the faeces or stool can be shed into the environment and subsequently contaminate various animal- and plant-based foods. Direct transmission between animals and humans and from humans to humans are also possible. Certain toxin producing variants of E. coli can cause serious diseases in animals and humans.

E. coli variants that can form Shiga toxins are of particular importance for humans. These are abbreviated as STEC. STEC, which cause diseases in humans, are referred to as entero-hemorrhagic E. coli (EHEC). The symptoms of an infection with STEC are initially gastrointestinal.

The possible severity of the disease ranges from watery to bloody diarrhea. In adults, the course of the disease can also proceed without symptoms. A particularly severe complication is the hemolytic-uremic syndrome (HUS). HUS is a disease that manifests in acute kidney failure, blood coagulation disorders and destruction of the red blood cells and can lead to death in individual cases. This form of the disease affects particularly sensitive groups of people, such as young chil-dren.

Spain – New regulations on food safety applied to retail trade

ACSA

Published in the BOE Royal Decree 1021/2022, of December 13, which regulates certain hygiene requirements for the production and marketing of food products in retail establishments.

This royal decree establishes measures for the correct application throughout the State of the EU regulations on food safety, with the aim that its implementation is homogeneous throughout the territory and also makes use of flexibility provisions to favor the sustainability of the food system.

The new text compiles in a single regulation the requirements for meat and derivatives, for fishery products, raw milk, foods made with eggs, prepared meals, thus repealing the specific regulations for these food groups and that were prior to the hygiene pack.

In addition, it incorporates in its articles the regulation of the preparation of meals in private homes, the requirements in the tasting areas, vending machines, the access of pets to these establishments and establishes measures against food waste and the use of reusable containers. .

The bill regulates, among others, hygiene requirements such as temperature and establishes guidelines for freezing, thawing and refreezing operations and specific requirements for prepared meals.

In the case of large pieces of fruit, such as watermelon, melon, pineapple, cut into halves or quarters, it will be allowed to keep them for a while at room temperature.

Table 1. Temperature requirements of food products

Food refrigeration temperature

1. Meat of domesticated ungulates and of wild or farmed large game, except rats.

Equal to or less than 7 °C.
2. Carcasses of domestic ungulates, farmed and wild game, poultry and lagomorphs. Equal to or less than 3 °C.
3. Meat of poultry, lagomorphs, small wild game and rats. Equal to or less than 4 °C.
4. Meat preparations. Equal to or less than 4 °C.
5. Minced meat. Equal to or less than 2 °C.
6. Live bivalve molluscs and fishery products that are kept alive. Temperature that does not negatively affect its safety and viability.
7. Fresh fishery products, unprocessed thawed fishery products, cooked and refrigerated crustaceans and molluscs. Temperature close to that of melting ice (0-4 °C).
8. Raw milk. 1-4°C
9. Filled pastry products (unless stable at room temperature). Equal to or less than 4 °C.
10. Cut or peeled fruits, cut or peeled vegetables and unpasteurized juices ready for consumption and made in the retail trade. Equal to or less than 4 °C.
11. Frozen or deep-frozen foods. Equal to or less than –18 °C.

USA – CDC Update – Multistate Norovirus Outbreak Linked to Raw Oysters from Texas

CDC

Food Borne Illness - Norovirus -CDC Photo

The Centers for Disease Control and Prevention (CDC), the U.S. Food and Drug Administration (FDA), the Texas Department of State Health Services, and other public health partners, are investigating a multistate outbreak of norovirus illnesses linked to raw oysters from Texas.

FDA Advises Restaurants, Retailers and Consumers to Avoid Potentially Contaminated Oysters from Harvest Area TX 1, Texas | FDA

Fast Facts

Illnesses: 298 illnesses* have been reported as of December 20, 2022.

States affected: Alabama (AL), Florida (FL), Georgia (GA), Louisiana (LA), Mississippi (MS) North Carolina (NC), Tennessee (TN), Texas (TX)

Recall: Yes DSHS Recalls Oysters Harvested in Area of Southeastern Galveston Bay | Texas DSHS

Investigation Status: Active

*This number is an estimate based on the information we have at this time. CDC is working with state and local partners and will update this number as more information is gathered.

Raw Oysters and Norovirus

If eaten raw, oysters and other filter-feeding shellfish can contain viruses and bacteria that can cause illness or death. Anyone who consumes raw shellfish is at risk of contracting norovirus. Children younger than five years old, the elderly, and those people with weakened immune systems are more likely to have severe infections. Food contaminated with norovirus may look, smell, or taste normal. To avoid food poisoning from oysters, cook them well to a temperature of at least 145 degrees F.

USA -FDA Core Investigation Table -Investigations of Foodborne Illness Outbreaks

FDA

Active Investigations

Date
Posted
Ref Pathogen
or
Cause of
Illness
Product(s)
Linked to
Illnesses

(if any)
Total
Case
Count

Status
Outbreak/
Event
Status
11/16/22 1113 Salmonella

Typhimurium

Not Yet
Identified
274 Active Ended
See Advice
11/9/22 1127 Listeria
monocytogenes
Enoki Mushrooms See
CDC’s
Investigation
Notice
Active Ongoing
See
CDC’s
Investigation
Notice