Research – Evaluation of food safety knowledge and microbial status of food contact surfaces in schools

Wiley Online Library 



The purpose of this study was to evaluate the hygiene of food contact surfaces in 37 high school kitchens in order to identify the factors involved in the deficiencies found. The microbial status of kitchen surfaces (mesophilic aerobic bacterial count) and the hygienic sanitary knowledge (assessed by a questionnaire) of 158 food handlers were evaluated. The microbial count was determined by conventional plating methods and by a redox potential‐based testing method. Large differences regarding microbial count on food contact surfaces were found among the kitchens. In connection with the unfavorable results, the food hygiene knowledge level of kitchen workers was unsatisfactory. The microbial profile assessment of a catering unit largely depends on the efficiency of the food safety management system used, therefore the level of microbial contamination can be used as an indicator. The results showed that the hygienic‐sanitary knowledge of food handlers highly affects food safety standards in public kitchens.

Practical applications

The microbial profile of food contact surfaces is a suitable indicator of a food safety management system with objective results that are easily interpreted. Recurrent unsatisfactory results can indicate failures in some or all areas of the food safety management systems. This study shows that in most cases these failures are related to the food handler’s knowledge. The improvement of food handler’s professional knowledge is an effective way to improve food safety levels in catering units.

Research – Molecular epidemiology of isolates with multiple mcr plasmids from a pig farm in Great Britain: the effects of colistin withdrawal in the short and long term




The environment, including farms, might act as a reservoir for mobile colistin resistance (mcr) genes, which has led to calls for reduction of usage in livestock of colistin, an antibiotic of last resort for humans.


To establish the molecular epidemiology of mcr Enterobacteriaceae from faeces of two cohorts of pigs, where one group had initially been treated with colistin and the other not, over a 5 month period following stoppage of colistin usage on a farm in Great Britain; faecal samples were also taken at ∼20 months.


mcr-1 Enterobacteriaceae were isolated from positive faeces and was WGS performed; conjugation was performed on selected Escherichia coli and colistin MICs were determined.


E. coli of diverse ST harbouring mcr-1 and multiple resistance genes were isolated over 5 months from both cohorts. Two STs, from treated cohorts, contained both mcr-1 and mcr-3 plasmids, with some isolates also harbouring multiple copies of mcr-1 on different plasmids. The mcr-1 plasmids grouped into four Inc types (X4, pO111, I2 and HI2), with mcr-3 found in IncP. Multiple copies of mcr plasmids did not have a noticeable effect on colistin MIC, but they could be transferred simultaneously to a Salmonella host in vitro. Neither mcr-1 nor mcr-3 was detected in samples collected ∼20 months after colistin cessation.


We report for the first known time on the presence in Great Britain of mcr-3 from MDR Enterobacteriaceae, which might concurrently harbour multiple copies of mcr1 on different plasmids. However, control measures, including stoppage of colistin, can successfully mitigate long-term on-farm persistence.

RASFF Alerts – Salmonella – Chicken Kebab Meat – Lettuce – B-Eggs – Chicken Fillet – Chilled Beef – Sesame Seeds -Water Spinach -Pandan Leaf

kswfoodworld food safety poisoning

RASFF-Salmonella (presence /25g) in frozen chicken kebab meat from Poland in Poland

RASFF -Salmonella in lettuce from the Netherlands in the Netherlands

RASFF-Salmonella (presence) in organic B-eggs from Germany in Germany

RASFF-Salmonella enterica ser. Enteritidis (presence /25g) in frozen chicken fillet from Poland in France

RASFF-Salmonella (presence in /10g) in chilled beef from Belgium in Belgium

RASFF-Salmonella (presence /25g) in sesame seeds from Sudan in Greece

RASFF-Salmonella (presence /25g) in sesame seeds from Sudan in Greece

RASFF-Salmonella group D (present /25g) in water spinach from Thailand in Finland

RASFF-Salmonella enterica subsp. salamae (II) (presence /25g) in pandan leaf (Pandanus amaryllifolius) from Thailand in Finland

RASFF Alert – Campylobacter – Chilled Chicken Breast

kswfoodworld food safety poisoning

RASFF– Campylobacter coli (100 <–> 8000 CFU/g) and Campylobacter jejuni (100 <–> 8000 CFU/g) in chilled chicken breasts from France in Denmark

RASFF Alert – E.coli – Cooked Mussel Meat

kswfoodworld food safety poisoning

RASFF-too high count of Escherichia coli (160 MPN /g) in frozen cooked mussel meat from Chile in Bulgaria

RASFF Alerts – Aflatoxin – Brazil Nuts – Almonds – Pistachios

kswfoodworld food safety poisoning

RASFF-aflatoxins (B1 = 34; Tot. = 74 µg/kg – ppb) in brazil nuts from Bolivia, via Germany in France

RASFF -aflatoxins (B1 = 8; Tot. = >24 µg/kg – ppb) in unshelled almonds from the United States in Spain

RASFF -aflatoxins (B1 = 19; Tot. = 21 µg/kg – ppb) in roasted and salted pistachio nuts from Belgium, with raw material from the United States in Belgium

RASFF Alert -Foodborne Outbreak caused by Salmonella in goat’s raw milk cheese

kswfoodworld food safety poisoning

RASFF-foodborne outbreak caused by Salmonella (presence /25g) in goat’s raw milk cheese from France in France