Category Archives: Microbiology

Research – The use of antifungal oat‐sourdough lactic acid bacteria to improve safety and technological functionalities of the supplemented wheat bread

Wiley Online

In the present study, predominant lactic acid bacteria (LAB) were isolated from oat sourdough. Then, the isolates were screened based on their in vitro antifungal activity. Subsequently, biotechnological capabilities of the selected LAB were evaluated in wheat bread supplemented with controlled fermented oat containing the isolate. Pediococcus pentosaceus was molecular identified as predominant antifungal isolate. Based on our results, fermented oat not only significantly (p < .05) reduced the mold expansion on the produced breads, but also improved the quality attributes of the product. Crumb porosity and antioxidant capacity of the supplemented bread revealed the positive effects of the isolate on textural and functional characteristics of the enriched bread. Wheat bread supplemented with controlled fermented oat had also the highest 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity compared to the other samples. Accordingly, the potential applications of Ppentosaceus isolate as profunctional starter culture in processing of mixed wheat‐oat sourdough bread were verified.

Research – Food safety against milk mycotoxins

All About Feed

According to the FAO and USDA, 25% of world agricultural crops are contaminated with some kind of mycotoxin and, as a consequence over, 532 million/ton of grains are contaminated. Considering that the base of animal feed is constituted by grains and cereals, most of this production may be contaminated with mycotoxins, which is very alarming. Economic consequences are significant and are not limited only to the added value of raw materials, but also to losses related to the loss of animal productivity, since the spread of mycotoxins occurs throughout the food chain, leading to contamination of final products, such as meat, milk and eggs.

Aflatoxins are secondary metabolites produced by Aspergillus parasiticus, Aspergillus flavus and the uncommon Aspergillus nomius. They appear in foods in the forms of aflatoxin B1 (AFB1), B2, G1 and G2 and in milk in the oxidative forms M1 and M2.

USA – FDA Alert: Certain Lots of Sportmix Pet Food Recalled for Potentially Fatal Levels of Aflatoxin

FDA

Fast Facts

  • FDA is alerting pet owners and veterinary professionals about certain Sportmix pet food products (see list below) manufactured by Midwestern Pet Foods, Inc. that may contain potentially fatal levels of aflatoxin.
  • FDA is aware of at least 28 deaths and 8 illnesses in dogs that ate the recalled product.
  • This is an ongoing investigation. Case counts and the scope of this recall may expand as new information becomes available.
  • Aflatoxin is a toxin produced by the mold Aspergillus flavus, which can grow on corn and other grains used as ingredients in pet food. At high levels, aflatoxin can cause illness and death in pets.
  • Pets experiencing aflatoxin poisoning may have symptoms such as sluggishness, loss of appetite, vomiting, jaundice (yellowish tint to the eyes or gums due to liver damage), and/or diarrhea. In severe cases, this toxicity can be fatal. In some cases, pets may suffer liver damage but not show any symptoms.
  • Pet owners should stop feeding their pets the recalled products listed below and consult their veterinarian, especially if the pet is showing signs of illness.  The pet owner should remove the food and make sure no other animals have access to the recalled product.
  • FDA is asking veterinarians who suspect aflatoxin poisoning in their patients to report the cases through the Safety Reporting Portal or by calling their local FDA Consumer Complaint Coordinators. Pet owners can also report suspected cases to the FDA.

What is the Problem?

On December 30, 2020, Midwestern Pet Food, Inc. announced a recall of certain lots of Sportmix pet food products after FDA was alerted about reports of at least 28 dogs that have died and eight that have fallen ill after consuming the recalled Sportmix pet food. Multiple product samples were tested by the Missouri Department of Agriculture and found to contain very high levels of aflatoxin. Aflatoxin is a toxin produced by the mold Aspergillus flavus and at high levels it can cause illness and death in pets. The toxin can be present even if there is no visible mold.

FDA is issuing this advisory to notify the public about the potentially fatal levels of aflatoxin in pet food products that may still be on store shelves, online, or in pet owners’ homes.

FDA is conducting follow-up activities at the manufacturing facility.

This is a developing situation and the FDA will update this page with additional information as it becomes available.

What are the Symptoms of Aflatoxin Poisoning in Pets?

Pets are highly susceptible to aflatoxin poisoning because, unlike people, who eat a varied diet, pets generally eat the same food continuously over extended periods of time. If a pet’s food contains aflatoxin, the toxin could accumulate in the pet’s system as they continue to eat the same food.

Pets with aflatoxin poisoning may experience symptoms such as sluggishness, loss of appetite, vomiting, jaundice (yellowish tint to the eyes, gums or skin due to liver damage), and/or diarrhea. In some cases, this toxicity can cause long-term liver issues and/or death. Some pets suffer liver damage without showing any symptoms. Pet owners whose pets have been eating the recalled products should contact their veterinarians, especially if they are showing signs of illness.

There is no evidence to suggest that pet owners who handle products containing aflatoxin are at risk of aflatoxin poisoning. However, pet owners should always wash their hands after handling pet food.

What Products are Involved?

On December 30, 2020, Midwest Pet Food, Inc. announced a recall of nine total lots of Sportmix pet food products. FDA and the Missouri Department of Agriculture are working with the firm to determine whether any additional products may have been made with the same ingredients containing potentially fatal levels of aflatoxin. As new information becomes available, this product list may continue to expand.

The list of recalled dry pet food products announced by Midwestern Pet Food, Inc. on December 30, 2020 is:

  • Sportmix Energy Plus, 50 lb. bag
    • Exp 03/02/22/05/L2
    • Exp 03/02/22/05/L3
    • Exp 03/03/22/05/L2
  • Sportmix Energy Plus, 44 lb. bag
    • Exp 03/02/22/05/L3
    • Sportmix Premium High Energy, 50 lb. bag
    • Exp 03/03/22/05/L3
  • Sportmix Premium High Energy, 44 lb. bag
    • Exp 03/03/22/05/L3
    • Sportmix Original Cat, 31 lb. bag
    • Exp 03/03/22/05/L3
  • Sportmix Original Cat, 15 lb. bag
    • Exp 03/03/22/05/L2
    • Exp 03/03/22/05/L3

Lot code information may be found on the back of bag and will appear in a three-line code, with the top line in format “EXP 03/03/22/05/L#/B###/HH:MM”

Example product label demonstrating location and format of lot code information.

The affected products were distributed to online retailers and stores nationwide within the United States.

What Do Retailers Need to Do?

Don’t sell or donate the affected pet food products. Contact the manufacturer for further instructions. The FDA also encourages retailers to contact consumers who have purchased recalled products, if they have the means to do so (such as through shopper’s card records or point-of-sale signs).

What Do Pet Owners Need to Do?

If your pet has symptoms of aflatoxin poisoning, contact a veterinarian immediately. Even pets without symptoms may have suffered liver damage, so you may want to contact your veterinarian if your dog has eaten any of the recalled products. Provide a full diet history to your veterinarian. You may find it helpful to take a picture of the pet food label, including the lot number.

Don’t feed the recalled products to your pets or any other animal. Contact the company listed on the package for further instructions or throw the products away in a way that children, pets and wildlife cannot access them. Sanitize pet food bowls, scoops, and storage containers using bleach, rinsing well afterwards with water, and drying thoroughly.

There is no evidence to suggest that pet owners who handle products containing aflatoxin are at risk of aflatoxin poisoning. However, pet owners should always wash their hands after handling any pet food.

You can report suspected illness to the FDA electronically through the Safety Reporting Portal or by calling your state’s FDA Consumer Complaint Coordinators. It’s most helpful if you can work with your veterinarian to submit your pet’s medical records as part of your report. For an explanation of the information and level of detail that would be helpful to include in a complaint to the FDA, please see How to Report a Pet Food Complaint.

What Do Veterinarians Need to Do?

The FDA urges veterinarians treating aflatoxin poisoning to ask their clients for a diet history. We also welcome case reports, especially those confirmed through diagnostic testing. You can submit these reports electronically through the FDA Safety Reporting Portal or by calling your state’s FDA Consumer Complaint Coordinators. For an explanation of the information and level of detail that would be helpful to include in a complaint to the FDA, please see How to Report a Pet Food Complaint.

The information in this release reflects the FDA’s best efforts to communicate what it has learned from the manufacturer and parties involved in the investigation. The agency will update this page as more information becomes available. 

Additional Information

RASFF Alerts – Aflatoxin – Dried Figs – Organic Peanut Kernels – Roasted and Salted Watermelon Seeds

RASFF

aflatoxins (B1 = 81 µg/kg – ppb) in dried figs from Spain in Spain

RASFF

aflatoxins (B1 = 54; Tot. = 64 µg/kg – ppb) in organic peanut kernels from Egypt in the Netherlands

RASFF

aflatoxins (B1 = 13.7 mg/kg – ppm) and ochratoxin A (11.4 mg/kg – ppm) in roasted and salted watermelon seeds from Turkey in Germany

RASFF

aflatoxins (B1 = 36; Tot. = 90 µg/kg – ppb) in organic dried figs from Turkey in the Netherlands

RASFF

aflatoxins (B1 = 53; Tot. = 55 µg/kg – ppb) in dried figs from Turkey in France

RASFF

aflatoxins (B1 = 17; Tot. = 18 µg/kg – ppb) in dried figs from Turkey in France

RASFF

aflatoxins (B1 = 17; Tot. = 22 µg/kg – ppb) in dried figs from Turkey

RASFF

aflatoxins (B1 = 48; Tot. = 109 µg/kg – ppb) in dried figs from Turkey in France

RASFF Alerts – E.coli – Chilled Burrata – Chilled Mussels

European Food Alerts

RASFF

high count of Escherichia coli (730000 CFU/g) in chilled burrata from Italy in France

RASFF

Escherichia coli (130,330,45,490,170 MPN/100g) in chilled mussels (Mitylus galloprovincialis) from Spain in Italy

RASFF Alert – Histamine – Chilled Tuna

European Food Alerts

RASFF

histamine (300.7 mg/kg – ppm) in chilled tuna (Thunnus albacares) from Sri Lanka, via the United Kingdom in Ireland

RASFF Alerts – Salmonella – Black Pepper – Chicken Legs -Chicken Tulips

European Food Alerts

RASFF

Salmonella enterica ser. Abaetetuba (presence /25g), Salmonella enterica ser. Glostrup (presence /25g) and Salmonella enterica ser. Kiambu (presence /25g) in black pepper from Brazil in Germany

RASFF

Salmonella enterica ser. Saintpaul (presence /25g) in black pepper from Brazil in Germany

RASFF

Salmonella (presence /25g) in black pepper from Brazil in the Netherlands

RASFF

Salmonella enterica ser. Enteritidis (presence /25g) in frozen chicken legs from Poland in Romania

RASFF

Salmonella enterica ser. Enteritidis (presence /25g) in frozen chicken tulips from Poland in France

RASFF Alerts – Mycotoxin – Ergot Alkaloids – Rye

RASFF

ergot (Claviceps purpurea) alkaloids (1069 mg/kg – ppm) in rye from Poland in Belgium

RASFF Alert – Animal Feed – Salmonella – Sunflower Meal

European Food Alerts

RASFF

Salmonella (presence /25g) in sunflower meal from Romania in Romania

Research – Listeriosis Caused by Persistence of Listeria monocytogenes Serotype 4b Sequence Type 6 in Cheese Production Environment

CDC

Abstract

A nationwide outbreak of human listeriosis in Switzerland was traced to persisting environmental contamination of a cheese dairy with Listeria monocytogenes serotype 4b, sequence type 6, cluster type 7488. Whole-genome sequencing was used to match clinical isolates to a cheese sample and to samples from numerous sites within the production environment.