Category Archives: Clostridium perfringens

USA – Clostridium Perfringens Linked to BBQ

Marler Blog

An outbreak of likely Clostridium perfringens was investigated by the Colorado Department of Public Health and Environment and the Jefferson County Public Health starting on August 22, 2023. That day, a school nurse at Evergreen County Day School reported 40 or so illnesses following a school event on August 21, 2023. The outbreak was given the state ID “2023-30-129.”

Research – Identify toxin-producing bacteria more quickly

ANSES

Improving the identification of toxin-producing bacteria is a real challenge for understanding food poisoning episodes. As part of the Joint European “One Health” Program coordinated by ANSES, the agency coordinated a European collaborative project on the toxin-producing bacteria which cause the most collective foodborne illnesses (TIAC).

Staphylococcus aureus, Bacillus cereus and Clostridium perfringens were at the heart of the European TOX-Detect project. This trio was not chosen at random: they are the toxin-producing bacteria most frequently involved in collective foodborne illness (TIAC) . According to the European Union Zoonoses Report One Health 2021 published by the European Food Safety Authority (EFSA), bacterial toxins are the second leading cause of TIAC after bacteria themselves (17%). The project, coordinated by ANSES, began in 2018 for a duration of 3 years. It was funded by the One Health EJP program and involved the Institut Pasteur, INRAE ​​as well as various partners from several European countries.

 “  Depending on the bacterial strains, the expression of virulence factors is not the same. These virulence factors are, for example, the presence of adhesion proteins or the production of toxins, in food or in the body. They serve the bacteria to counter the defenses that the host could put up against them, explains Yacine Nia, co-coordinator of the project and deputy head of unit of the Staphylococcus, Bacillus, Clostridium (SBCL) unit, of the food safety laboratory. of Anses. The ability of the bacteria to harm the body will be higher or lower depending on these virulence factors.  »

Most poisonings caused by the toxins of the three bacteria studied cause gastrointestinal symptoms (nausea, vomiting, abdominal pain, diarrhea). Deaths may occur, especially in the most sensitive subjects.

Research – High Prevalence of Multidrug-Resistant, Biofilm-Forming Virulent Clostridium perfringens in Broiler Chicken Retail Points in Northeast India

MDPI

Abstract

In light of the significant public health and food safety implications associated with Clostridium perfringens, this study aimed to isolate and characterize C. perfringens in samples obtained from broiler chicken retail points in Meghalaya, northeastern India. A total of 280 samples comprising meat, intestinal contents, water, and hand swabs were processed to detect contamination by C. perfringens. The isolates were subjected to toxinotyping, antimicrobial susceptibility testing, and biofilm-forming ability test. The overall occurrence of C. perfringens was 22.5% (17.74–27.85, 95% CI) with the highest recovery from intestine samples (31%; 22.13–41.03, 95% CI), followed by meat (23%, 15.17–32.49, 95% CI) and water samples (18%, 8.58–31.44, 95% CI). Type A was the predominant toxinotype (71.43%, 58.65–82.11, 95% CI), followed by Type A with beta2 toxin (17.46%, 9.05–29.10, 95% CI), Type C (7.94%, 2.63–17.56, 95% CI), and Type C with beta2 toxin (3.17%, 0.39–11.0, 95% CI). Nearly all (95.24%) isolates were multidrug resistant and 68.25% were biofilm formers. The predominance of multidrug-resistant and virulent Type A and Type C C. perfringens in retail broiler meat and intestines in the tribal-dominated northeastern region of India is of great concern from food safety and public health perspectives.

RASFF Alert – Animal Feed – Clostridium perfringens – Fish Meal

RASFF

Clostridium perfringens in fish meal from Portugal in Italy

USA – Clostridium perfringens or Bacillus cereus outbreak sickens 34 at Tacos El Guero

Food Poison Journal

Summary

Public Health is investigating an outbreak of gastrointestinal illness associated with a private event catered by a Tacos El Guero food truck on September 14, 2023.  Symptoms and timing of illness onsets were suggestive of a bacterial toxin, such as Clostridium perfringens or Bacillus cereus.
The exact food or drink that caused the illnesses has not been identified, though this is not uncommon for outbreaks associated with a bacterial toxin.

Illnesses

Public Health identified 34 sick people that developed one or more symptoms consistent with bacterial toxin, including diarrhea, abdominal cramping, and nausea. Illness onset dates ranged from September 14, 2023, to September 15, 2023.

Public Health actions

On September 15, 2023, a group reported the outbreak to Public Health after eating together on September 14, 2023. Public Health gathered information about symptoms and when people became ill.

Environmental Health investigators visited the mobile food trucks and restaurant on September 18, 2023. Investigators identified potential risk factors for bacterial toxin growth including inadequate refrigeration and improper cooling of food. We also observed inadequate equipment improper reheating, and lack of managerial oversight. Additionally, food at this event was served out of an unpermitted food truck. Corrective actions were discussed at the time of the visit. Based on unsafe food handling practices identified during the investigation, environmental health investigators closed the restaurant on September 18, 2023. Environmental Health investigators will revisit the facility to ensure adoption of safe food handling practices prior to allowing the restaurant to reopen.

Research – Microbiological Quality and Safety of Fresh Quail Meat at the Retail Level

MDPI

Abstract

The objective of this study was to evaluate the microbiological quality and safety of 37 fresh quail meats. Mesophiles, Pseudomonas spp., Enterobacteriaceae, and staphylococci counts were 5.25 ± 1.14, 3.92 ± 1.17, 3.09 ± 1.02, and 2.80 ± 0.64 log CFU/g, respectively. Listeria monocytogenes was detected in seven samples (18.92%). Campylobacter jejuni was detected in one sample (2.70%). Clostridium perfringens was not detected in any sample. The dominant bacteria were Pseudomonas spp. (30.46%), Micrococcaceae (19.87%), lactic acid bacteria (14.57%), and Enterobacteriaceae (11.92%). Brochotrix thermosphacta and enterococci were isolated to a lesser extent, 7.28% and 1.99%, respectively. The dominant Enterobacteriaceae found were Escherichia coli (42.53%). ESBL-producing E. coli was detected in one sample (2.70%), showing resistance to 16 antibiotics. Sixteen different Staphylococcus spp. and three Mammaliicoccus spp. were identified, the most common being S. cohnii (19.86%) and M. sciuri (17.02%). S. aureus and S. epidermidis were also found in one and four samples, respectively. Methicillin-resistant M. sciuri and S. warneri were found in 13.51% and 10.81% of quail samples, respectively. These bacteria showed an average of 6.20 and 18.50 resistances per strain, respectively. The high resistance observed in ESBL-producing E. coli and methicillin-resistant S. warneri is of special concern. Measures should be adopted to reduce the contamination of quail meat.

France sees a rise in outbreaks in 2021

Food Safety News

The number of outbreaks in France went up in 2021 but was still below 2018 and 2019 levels.

Figures from Santé publique France show there were 1,309 outbreaks affecting 11,056 people in 2021. A total of 512 people went to hospitals and 16 died.

Reported outbreaks rose from 1,010 in 2020 when figures were strongly impacted by the COVID-19 pandemic, according to officials.

For 327 incidents in 2021, a pathogen could be microbiologically confirmed in food or in at least one sick person per outbreak. A pathogen was suspected without microbiological confirmation for 734 outbreaks.

16 deaths in outbreaks
The most frequently microbiologically confirmed pathogen was Salmonella 147 times. It was mostly Salmonella Enteritidis, followed by monophasic Salmonella Typhimurium but the type was not known in 92 incidents. Confirmed Salmonella outbreaks were responsible for 935 patients and 166 hospitalizations.

Campylobacter caused 52 outbreaks with 178 patients and Bacillus cereus had 46 with 665 patients. Nineteen norovirus outbreaks sickened 493 and 11 histamine outbreaks affected 35 people.

Seven outbreaks were due to E. coli, six to Yersinia enterocolitica, four to Shiga toxin-producing E. coli (STEC), and one each because of Listeria monocytogenes, Clostridium botulinum, and Shigella.

The pathogen was identified in patients for 208 outbreaks, of which 143 were confirmed with Salmonella. Identification of the agent took place in food samples for 89 epidemics with 67 confirmed as Bacillus cereus, Staphylococcus aureus and Clostridium perfringens.

The top suspected pathogens based on epidemiological and clinical information, but not microbiologically confirmed, were the toxins Bacillus cereus, Staphylococcus aureus and Clostridium perfringens. For these three pathogens, 3,787 patients and 169 hospital trips were recorded.

Research – Clostridium perfringens—Opportunistic Foodborne Pathogen, Its Diversity and Epidemiological Significance

MDPI

CDC Clost perf

Abstract

The C. perfringens species is associated with various environments, such as soils, sewage, and food. However, it is also a component of the gastrointestinal (GI) microflora (i.e., microbiota) of sick and healthy humans and animals. C. perfringens is linked with different systemic and enteric diseases in livestock and humans, such as gas gangrene, food poisoning, non-foodborne diarrhoea, and enterocolitis. The strains of this opportunistic pathogen are known to secrete over 20 identified toxins that are considered its principal virulence factors. C. perfringens belongs to the anaerobic bacteria community but can also survive in the presence of oxygen. The short time between generations, the multi-production capability of toxins and heat-resistant spores, the location of many virulence genes on mobile genetic elements, and the inhabitance of this opportunistic pathogen in different ecological niches make C. perfringens a very important microorganism for public health protection. The epidemiological evidence for the association of these strains with C. perfringens–meditated food poisoning and some cases of non-foodborne diseases is very clear and well-documented. However, the genetic diversity and physiology of C. perfringens should still be studied in order to confirm the importance of suspected novel virulence traits. A very significant problem is the growing antibiotic resistance of C. perfringens strains. The aim of this review is to show the current basic information about the toxins, epidemiology, and genetic and molecular diversity of this opportunistic pathogen.

Research – Prevalence and Characterisation of Clostridium perfringens Isolates in Food-Producing Animals in Romania

MDPI

Abstract

The aim of the current study was to investigate the prevalence of Clostridium perfringens (C. perfringens) recovered from animal faeces, as well as to determine the antimicrobial susceptibility of such isolates. A total of 14 (14/100; 14%) C. perfringens isolates were isolated from the 100 analysed samples (twelve recovered from faecal samples collected from pigs and two from veal calves’ faecal samples). The preponderant genotype was type A, with all isolates being cpa-positive. The most potent antimicrobial agents against C. perfringens proved to be vancomycin, rifampicin and lincomycin. A strong resistance to tetracycline (71.4%), penicillin (64.2%), erythromycin (42.8%) and enrofloxacin (35.7%) was also observed. To the best of our knowledge, this is the first analysis regarding the prevalence, characterization and antimicrobial susceptibility of C. perfringens in food-producing animals in Romania, adding further evidence for the probable role of animals as a source of resistant C. perfringens strains.

Catalonia – Alert for the presence of Clostridium perfringens in lots of cinnamon from Vietnam

ACSA

cinnamon much of the alert

The Spanish Agency for Food Safety and Nutrition has communicated to the competent authorities of the autonomous communities and the Center for Coordination of Health Alerts and Emergencies (CCAES), through the Coordinated  System of Rapid Information Exchange  (SCIRI), a alert, transferred by the health authorities of the Community of Madrid, regarding the presence of Clostridium perfringens in cinnamon molta of the Especias Pedroza brand.

The data of the product involved are:

  • Product Name:  Ground Cinnamon
  • Brand/commercial name : Especias Pedroza
  • Product appearance: Plastic packaging
  • Batch numbers and best before dates: 
    • A220079, 12/31/2023
    • A222605, 02/28/2026
  • Unit weight:  700 g
  • Temperature: Ambient.

The withdrawal of the affected products from the marketing channels will be verified by the competent authorities.

People who have products affected by this alert at home are advised to refrain from consuming them and return them to the point of purchase.