Category Archives: Microbiological Risk Assessment

India – 100 people suffer food poisoning after baptism ceremony in Kerala; case filed, probe underway

India Today

Over 100 people suffered food poisoning after consuming food served at a baptism ceremony in Kerala last Thursday. Police have registered a case and are conducting further investigations.

 

Australia – At Least 70 Contract Salmonella Following Conference at the Crown Plaza Terrigal Pacific Hotel

Food Poisoning News

A hotel on the New South Wales Central Coast suffered from a severe food poisoning outbreak of Salmonella after approximately 70 out of 230 people who attended a two-day conference at the Crown Plaza Terrigal Pacific hotel on November 30th fell ill. A total of 27 out of the 70 illnesses have now been confirmed by medical professionals as cases of Salmonella.

The NSW Food Authority are currently investigating the cause of this outbreak and urges, “anyone who felt unwell or had concerns after the conference should seek medical care, get in touch with their local public health unit, or contact conference organizers, the Aboriginal Languages Trust.”

Authorities are continuing to investigate food that may have been contaminated at the conference, and for the time being, the hotel’s kitchen has voluntarily closed, though “it is required to do [so] under the Food Act of 2003.”

USA – Outbreak Investigation of Salmonella: Sprouts (December 2022)

FDA

The U.S. Food and Drug Administration (FDA), along with the Centers for Disease Control and Prevention (CDC) and state and local partners, is investigating a multistate outbreak of Salmonella Typhimurium infections. FDA’s traceback investigation is ongoing but has identified Sun Sprouts brand alfalfa sprouts grown by CW Sprouts Inc., doing business as (DBA) SunSprout Enterprises of Fremont, NE as a likely source of illnesses in this outbreak.

Based on the epidemiologic information provided by the CDC along with interviews conducted by state and local public health officials, the individuals identified in this outbreak reported consuming alfalfa sprouts prior to their illness onset, either at local restaurants or in their homes after purchasing from local grocery stores. Preliminary traceback evidence indicates that the restaurants and grocery stores identified in this outbreak received alfalfa sprouts from SunSprout Enterprises. Additionally, two of the individuals identified in this outbreak confirmed they had purchased Sun Sprouts brand alfalfa sprouts from their local grocery store. Whole Genome Sequencing (WGS) conducted on clinical samples collected from ill individuals identified 15 isolates that matched the outbreak strain: OK (1), NE (8), and SD (6). WGS for additional clinical isolates are still pending sequencing.

On December 29, 2022, SunSprout Enterprises initiated a voluntary recall of two lots (# 4211 and 5211) of raw alfalfa sprouts due to potential contamination with Salmonella. On the same day, SunSprout Enterprises expanded their voluntary recall to include two additional lots (# 3212, and 4212) of raw alfalfa sprouts. The raw alfalfa sprouts for all recalled lots (#4211, 5211, 3212, and 4212) are packaged in 4-ounce clamshells and 2.5 lb packages with best sold by dates between 12/10/2022 and 1/7/2023.

The investigation is ongoing to determine the source of contamination and whether additional products are linked to illnesses. FDA will update this advisory should additional consumer safety information become available.

Recommendation

Advice for consumers, restaurants, and retailers: Consumers, restaurants, and retailers should not eat, sell, or serve recalled Sun Sprouts brand alfalfa sprouts.

Consumers, restaurants, and retailers should check their refrigerators for recalled Sun Sprouts brand alfalfa sprouts with best by dates between 12/10/2022 and 1/7/2023. If you purchased or used recalled alfalfa sprouts, you should use extra vigilance in cleaning and sanitizing any surfaces that may have come in contact with these products, to reduce the risk of cross contamination.

If you or someone in your household ate Sun Sprouts alfalfa sprouts and have symptoms of salmonellosis, please contact your healthcare provider.

Suppliers and Distributors: Suppliers, distributors and others in the supply chain should not use, ship or sell recalled alfalfa sprouts grown by SunSprout Enterprises. If there has been potential cross-contamination or mixing of recalled alfalfa sprouts with products from other sources, suppliers and distributors should not use any comingled and potentially cross-contaminated product and should use extra vigilance in cleaning any surfaces and storage areas that may have come into contact with recalled alfalfa sprouts.


Product Images

Recalled products include raw alfalfa sprouts sold in 4-ounce clamshells and 2.5 Ib packages, with best by dates between 12/10/2022 and 1/7/2023.

Outbreak Investigation of Salmonella from Sprouts (December 2022) - Product Image

Case Count Map Provided by CDC

Outbreak Investigation of Salmonella from Sprouts (December 2022) - CDC Distribution Map as of 12/30/2022

Case Counts

Total Illnesses: 15
Hospitalizations: 2
Deaths: 0
Last illness onset: 12/13/2022
States with Cases: NE, OK, SD

Research – Effect of Ultra-High-Pressure Homogenization Processing on the Microbiological, Physicochemical, and Sensory Characteristics of Fish Broth

MDPI

Abstract

The effect of ultra-high-pressure homogenization (UHPH) treatments at 300 MPa at inlet temperatures (Ti) between 45 and 75 °C on the microbiological, physical, and sensorial characteristics of fish broth was evaluated. Before the application of UHPH treatments, different fish broth formulations were tested, selecting the formula with the best organoleptic and nutritional characteristics and the lowest cost, containing 45% monkfish heads and rock fish in the same proportion. The microbiological shelf-life of fish broth during cold storage at 4 and 8 °C was extended by a minimum of 20 days by applying UHPH treatments at inlet temperatures (Ti) between 45 and 65 °C. Fish broth UHPH-treated at Ti = 75 °C was microbiologically sterile during storage at 4 °C, 8 °C, and room temperature. Fish broth UHPH-treated was physically stable, significantly reducing the particle size. Color showed higher luminosity and lower yellowness as the inlet temperature increased. In fish broth UHPH-treated at Ti = 75 °C, selected for its microbiological stability, no differences were observed in the nutritional composition, antioxidant activity, and sensorial perception compared to untreated fish broth. Hence, UHPH treatments showed to be an alternative to preserving fish broth with an improved microbiological shelf-life and good sensorial characteristics.

Research – Surveillance of Human Cases of Salmonellosis, Campylobacteriosis, Listeriosis, and Hepatitis A in Campania (Southern Italy): Seven-Year Monitoring (2013–2019)

MDPI

Abstract

Foodborne infections cause illness and death every year worldwide. The aim of this study was to describe trends in 2013–2019 in the occurrence of human cases of salmonellosis, campylobacteriosis, listeriosis, and hepatitis A in the Campania region. Human case data were provided by the National Surveillance System of disease and were grouped by year, province, age group, and sex. Moreover, the number of people hospitalized was recorded. In the Campania region, the total number of confirmed human cases for the diseases investigated was 1924, with Hepatitis A and the Salmonellosis as the first most reported (1009 and 825 cases, respectively). The incidence rates of gastroenteritis under study were lower than those in Italy and European Union in the same period, with the exception of Hepatitis A whose incidence was higher than that recorded in Italy. Data on hospitalizations pointed out the onset of severe forms of infection also for listeriosis and campylobacteriosis, whose incidence was very low (27 and 63 cases, respectively). Unfortunately, no information on the foods implicated is available. Although probably underestimated, gastroenteritis due to foodborne agents still represents a burden in Campania, and continuous monitoring and implementation of the currently available regional surveillance system is required.

Research – Advances in science and risk assessment tools for Vibrio parahaemolyticus and V. vulnificus associated with seafood: meeting report

WHO

Globally, the Vibrio parahaemolyticus and Vibrio vulnificus represent important human pathogens associated with the consumption of seafood. In response to the requests for scientific advice from Codex Committee on Food Hygiene (CCFH), risk assessments for the pathogens V. vulnificus, V. cholerae, V. parahaemolyticus and guidance on methods for the detection of Vibrio spp. with seafood have been conducted and published previously by JEMRA. In order to provide an update on the state-of-the-art advice regarding risk assessment for V. parahaemolyticus and V. vulnificus in seafood, an expert meeting was convened.

Several critical developments in the last decade were subsequently noted by the expert working group: 1) The emergence of highly pathogenic strains; 2) In response to climate change, there has been a significant geographical spread regarding when and where these seafood-associated Vibrio infections; 3) Demographic considerations are very important; 4) A range of new approaches for best practice; and 5) A range of new methods, such as those utilising genomics and satellite imagery. This report describes the output of that expert meeting.

Research – Bacterial pathogens and climate change

The Lancet

Read the full article at the link above

A systematic analysis of the literature showed that 58% of infectious diseases in humans have been aggravated at some point by climatic hazards, such as changes in temperatures and water and food availability.

The leading pathogens responsible (eg, S aureus, E coli, K pneumoniae, S pneumoniae, and non-typhoidal Salmonella) have all been found to have climate-dependent reactions, causing an increase in their diffusion and even resistance to treatment.

 For example, sharp temperature decreases have been associated with an increased incidence of pneumonia, and increased morbidity in cold and humid weather.

 

Research -Control measures for Shiga toxin-producing Escherichia coli (‎STEC)‎ associated with meat and dairy products: meeting report

WHO

Although Shiga toxin-producing Escherichia coli (STEC) have been isolated from a variety of food production animals, they are most commonly associated with ruminants from which we derive meat and milk. Because of the widespread and diverse nature of ruminant-derived food production, coupled with the near ubiquity of STEC worldwide, there is no single definitive solution for controlling STEC that will work alone or in all situations. Instead, the introduction of multiple interventions applied in sequence, as a “multiple-hurdle scheme” at several points throughout the food chain (including processing, transport and handling) will be most effective.

This report summarizes the review and evaluation of interventions applied for the control of STEC in cattle, raw beef and raw milk and raw milk cheese manufactured from cows’ milk, and also evaluates available evidence for other small ruminants, swine and other animals. The information is presented from primary production, to the end of processing, providing the reader with information on the currently available interventions based on the latest scientific evidence.

This work was undertaken to support the development of guidelines for the control of STEC in beef, raw milk and cheese produced from raw milk by the Codex Committee on Food Hygiene (CCFH).

Research – Listeria monocytogenes—How This Pathogen Uses Its Virulence Mechanisms to Infect the Hosts

MDPI

Abstract

Listeriosis is a serious food-borne illness, especially in susceptible populations, including children, pregnant women, and elderlies. The disease can occur in two forms: non-invasive febrile gastroenteritis and severe invasive listeriosis with septicemia, meningoencephalitis, perinatal infections, and abortion. Expression of each symptom depends on various bacterial virulence factors, immunological status of the infected person, and the number of ingested bacteria. Internalins, mainly InlA and InlB, invasins (invasin A, LAP), and other surface adhesion proteins (InlP1, InlP4) are responsible for epithelial cell binding, whereas internalin C (InlC) and actin assembly-inducing protein (ActA) are involved in cell-to-cell bacterial spread. L. monocytogenes is able to disseminate through the blood and invade diverse host organs. In persons with impaired immunity, the elderly, and pregnant women, the pathogen can also cross the blood–brain and placental barriers, which results in the invasion of the central nervous system and fetus infection, respectively. The aim of this comprehensive review is to summarize the current knowledge on the epidemiology of listeriosis and L. monocytogenes virulence mechanisms that are involved in host infection, with a special focus on their molecular and cellular aspects. We believe that all this information is crucial for a better understanding of the pathogenesis of L. monocytogenes infection.

Research – Variability in Cold Tolerance of Food and Clinical Listeria monocytogenes Isolates

MDPI

Abstract

The aim of this study was to investigate the level of strain variability amongst food and clinical Listeria monocytogenes isolates growing at low temperatures (4 and 7 °C) in both laboratory media and real food matrices. Isolates (n = 150) grown in laboratory media demonstrated a large variation in growth profiles measured using optical density.
Overall, it was noted that clinical isolates exhibited a significantly higher growth rate (p ≤ 0.05) at 7 °C than the other isolates. Analysis of variance (ANOVA) tests of isolates grouped using Multi Locus Sequence Typing (MLST) revealed that clonal complex 18 (CC18) isolates were significantly (p ≤ 0.05) faster growing at 4 °C than other CC-type isolates while CC101, CC18, CC8, CC37 and CC14 were faster growing than other CC types at 7 °C. Euclidean distance and Ward method-based hierarchical clustering of mean growth rates classified 33.33% of isolates as faster growing.
Fast and slow growing representative isolates were selected from the cluster analysis and growth rates were determined using plate count data in laboratory media and model food matrices. In agreement with the optical density experiments, CC18 isolates were faster and CC121 isolates were slower than other CC types in laboratory media, UHT milk and fish pie. The same trend was observed in chocolate milk but the differences were not statistically significant. Moreover, pan-genome analysis (Scoary) of isolate genome sequences only identified six genes of unknown function associated with increased cold tolerance while failing to identify any known cold tolerance genes. Overall, an association that was consistent in laboratory media and real food matrices was demonstrated between isolate CC type and increased cold tolerance.