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Category Archives: Zearalenone
Research – Mycotoxins in Food: Cancer Risks and Strategies for Control
Mycotoxins are toxic compounds produced by fungi such as Aspergillus, Penicillium, and Fusarium, contaminating various food crops and posing severe risks to food safety and human health. This review discusses mycotoxins‘ origins, significance, and impact, particularly in relation to cancer risk. Major mycotoxins like aflatoxins, ochratoxins, fumonisins, zearalenone, and patulin are examined, along with their sources and affected foods. The carcinogenic mechanisms of these toxins, including their biochemical and molecular interactions, are explored, as well as epidemiological evidence linking mycotoxin exposure to cancer in high-risk populations. The review also highlights critical methodologies for mycotoxin detection, including HPLC, GC-MS, MS, and ELISA, and the sample preparation techniques critical for accurate analysis. Strategies for controlling mycotoxin contamination, both pre- and post-harvest, are discussed, along with regulations from organizations like the FAO and WHO. Current challenges in detection sensitivity, cost, and control effectiveness are noted. Future research is needed to develop innovative analytical techniques, improve control strategies, and address the influence of climate change on mycotoxin production. Finally, global collaboration and emerging technologies are essential for advancing mycotoxin control and enhancing food safety.
Research – Mycotoxin control in cereals: safe guarding food
The aim of this research was to review (and where possible improve/refine
current rapid screening methods for the mycotoxin T-2 and its metabolites in
cereals for human consumption.
Researchers engaged with the relevant sector of the cereal processing industry in
Ireland and the UK, and the relevant primary production sector, to gain detailed,
valuable information.
The research conducted was designed to clarify deficits in T-2 toxin control in the
relevant processing and primary production sectors, evaluate the robustness of
current analytical detection and control methods therein, augment same if deemed
necessary, and implement workable T-2 toxin control solutions at sectoral level.
Objectives
The specific objectives of the research project were to:
• Conduct a literature review of the most up-to-date scientific and
regulatory data for T-2 toxin and its metabolites, the specific environmental conditions required for their production, and the impact of climate change on their occurrence and distribution.
• Undertake a thorough analysis of the currently available commercial rapid analytical techniques for the screening of T-2 and HT-2 toxins in cereals for direct human consumption.
• Undertake a survey of the relevant processing and primary production sectors to determine what testing they currently conduct for mycotoxins, to fully understand the shortfalls in relation to mycotoxin monitoring and control within the industry.
Conduct a survey of oats and barley (n≥100) using mass spectrometry to identify contamination levels of, not only the mycotoxins of interest, i.e., T-2 and HT-2, but also all other regulated mycotoxins including aflatoxins, deoxynivalenol, zearalenone, fumonisins and ochratoxin A.
• Perform a thorough evaluation of a selected number of rapid test kits to ascertain fitness for purpose and determine how to adapt the most robust methods for better performance. If required, undertake a training workshop on the use of the best performing rapid testing kits.
• Engage with stakeholders to discuss the implications of the findings of
the project and produce a series of conclusions and recommendations
for stakeholders on the island of Ireland.
Belgium – Pearl Barley – pearl barley – from the Golden Lion brand – Mycotoxin – Zearalenon

Following a notification via the RASFF system (European Food and Feed rapid alert system), the FASFC withdraws from sale the product Pearl Barley (pearled barley) of the Golden Lion brand and recalls it from consumers due to too high a mycotoxin content (zearalenon).
The FASFC asks not to use this product and to return it to the point of sale from which it was purchased.
Product Description :
Product Name: Pearl Barley
Brand: Golden Lion
Minimum Durability Date (DDM): 12/31/2026
Lot number: 230601
Weight: 250g
The product was sold through various outlets, including:
| NV Kam Yuen Supermarket | 1000 | Brussels | Zwarte Lievevrouwstr. 2-4 |
| Hao Hao / Bvba Kim Phong | 1040 | Etterbeek | Avenue de la Chasse 182 |
| Raan Asia Bvba | 8000 | Brugge | Oostendsesteenweg 135 |
For any further information, you can contact the AFSCA contact point for consumers: 0800/13.550 or pointdecontact@afsca.be.
Posted in food contamination, food handler, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Poisoning, Food Quality, food recall, Food Safety, Food Safety Alert, Food Safety Management, Food Safety Regulations, Food Testing, Food Toxin, mold, Mold Toxin, Mould/Mold, Mycotoxin, Zearalenone
RASFF Alert- Mycotoxin – Zearalenone –
Zearalenone in pearl barley from China in Austria, Belgium, China, Cyprus, France, Germany, Hungary, Ireland, Netherlands, Slovakia, Slovenia, Sweden.
Posted in food contamination, food handler, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Poisoning, Food Quality, food recall, Food Safety, Food Safety Alert, Food Safety Management, Food Safety Regulations, Food Testing, Food Toxin, Mould Toxin, Mould/Mold, Mycotoxin, RASFF, Zearalenone
Research – Analysis of flour and rice shows high levels of harmful fungal toxins
By analyzing samples of flour and rice stored in homes in Ribeirão Preto, in the interior of the state of São Paulo (Brazil), researchers from the University of São Paulo (USP) found the presence of high levels of fungal toxins (mycotoxins). The study is published in the journal Food Research International.
The six toxins of concern were found in all the food samples analyzed: aflatoxins (AFs), fumonisins (FBs), zearalenone (ZEN), T-2 toxin, deoxynivalenol (DON), and ochratoxin A (OTA). In the case of the mycotoxins FBs, ZEN, and DON, the levels were above the tolerance limit set by the health authorities. This study was the first in Brazil to use biomarkers to characterize the risk associated with mycotoxins in the diet of children and adolescents.
Research – Mitigation of Mycotoxins in Food—Is It Possible?
Abstract
Among microorganisms found in food, fungi stand out because they are adaptable and competitive in a large range of water activities, temperatures, pHs, humidities and substrate types. Besides sporulating, some species are toxigenic and produce toxic metabolites, mycotoxins, under adverse biotic and abiotic variables. Microorganisms are inactivated along the food chain, but mycotoxins have stable structures and remain in ready-to-eat food. The most prevalent mycotoxins in food, which are aflatoxins, fumonisins, ochratoxin A, patulin, tenuazonic acid, trichothecenes and zearalenone, have maximum tolerable limits (MTLs) defined as ppb and ppt by official organizations. The chronic and acute toxicities of mycotoxins and their stability are different in a chemical family. This critical review aims to discuss promising scientific research that successfully mitigated levels of mycotoxins and focus the results of our research group on this issue. It highlights the application of natural antifungal compounds, combinations of management, processing parameters and emergent technologies, and their role in reducing the levels and bioaccessibility. Despite good crop management and processing practices, total decontamination is almost impossible. Experimental evidence has shown that exposure to mycotoxins may be mitigated. However, multidisciplinary efforts need to be made to improve the applicability of successful techniques in the food supply chain to avoid mycotoxins’ impact on global food insecurity.
Posted in Aflatoxin, Aflatoxin B1, Fumomisins, Fumonsins, mold, Mold Toxin, Mould/Mold, Mycotoxin, Ochratoxin, Ochratoxin A, Patulin, Zearalenone
Research – Chapter 12 – Mycotoxins in cereals
Abstract
Mycotoxins are toxic secondary metabolites of filamentous food-borne fungi that grow worldwide on a variety of cereals and other agricultural produce. Aflatoxins, ochratoxin A, fumonisins, trichothecenes, and zearalenone occur on cereals and lead to mycotoxicoses among humans, animals, and poultry. Some mycotoxins are carcinogenic, hepatotoxic, nephrotoxic, dermatoxic, genotoxic, teratogenic, immunotoxic, or estrogenic. Good agricultural practice involving sound preharvest practices such as optimum tillage, crop rotation, planting date, avoidance of drought stress, and optimal fertilization contributes to the production of healthy crops. Biocontrol agents, such as Aflasafe, and genetically modified insect-resistant maize, such as Bt maize, reduce mycotoxin formation in maize. Hygienic storage conditions contribute to the prevention of mycotoxin formation. Reliable analytical results are necessary for compliance with mycotoxin regulations and control procedures to facilitate the international trade of cereals. Compliance with regulations of international food safety authorities as well as public awareness of mycotoxins should enhance the protection of populations from the adverse health effects of mycotoxins.
Research- Determination of Mycotoxins in Plant-Based Meat Alternatives (PBMAs) and Ingredients after Microwave Cooking
Abstract
In this study, we investigate the role of microwave cooking in reducing mycotoxin contamination in plant-based food matrices, with a focus on veggie burgers (purchased and home-made) and their ingredients (soybean, potatoes, zucchini, carrots). Two different conditions were studied (Max–Min) that were 800 W for 60 s and 800 W for 90 s, respectively. The degradation patterns of aflatoxins (AFB1, AFB2, AFG1, AFG2), fumonisins (FB1, FB2, FB3), trichothecenes (T2, HT2, ZEA), and ochratoxin A (OTA) were studied. The extraction procedures were conducted with the QuEChERS extraction, and the analyses were conducted with liquid chromatography–tandem mass spectrometry (LC-MS/MS). Principal component analysis (PCA) showed that degradation under microwave cooking varies considerably across different food matrices and cooking conditions. This study provides valuable insights into the degradation of mycotoxins during microwave cooking and underscores the need for more research in this area to ensure food safety.
Research- Monitoring of Animal Feed Contamination by Mycotoxins: Results of Five Years of Official Control by an Accredited Italian Laboratory
Abstract
Mycotoxin contamination of animal feed is a complex issue in both animal wellness and food safety. The most diffused mycotoxins subject to the official control of animal feed are Aflatoxin B1 (AF), Zearalenone (ZEA), Deoxynivalenol (DON), Ochratoxin A (OCRA), Fumonisins (FUMO), and T-2/HT-2 toxins. This work describes the results of five years of monitoring focused on the evaluation of mycotoxin contamination of animal feed. Analytical determinations were carried out by means of accredited ELISA. The obtained results showed a non-alarming scenario, with several samples resulting as “non-compliant” according to the Maximum Residue Limits (MRLs) set in European Regulation No. 574/2011. Out of 722 analyzed samples coming from 2 Italian regions, Apulia and Basilicata, 14 samples were characterized by mycotoxin concentrations higher than related MRL; in particular, 5, 4, and 5 non-compliant samples for DON, AF, and ZEA, respectively. This study also evaluated the possible correlations between mycotoxin type and feed use with a special focus on animal sensitivity to mycotoxins.
Research – Fungal and Toxin Contaminants in Cereal Grains and Flours: Systematic Review and Meta-Analysis
Abstract
Cereal grains serve as the cornerstone of global nutrition, providing a significant portion of humanity’s caloric requirements. However, the presence of fungal genera, such Fusarium, Penicillium, Aspergillus, and Alternaria, known for their mycotoxin-producing abilities, presents a significant threat to human health due to the adverse effects of these toxins. The primary objective of this study was to identify the predominant fungal contaminants in cereal grains utilized in breadmaking, as well as in flour and bread. Moreover, a systematic review, including meta-analysis, was conducted on the occurrence and levels of mycotoxins in wheat flour from the years 2013 to 2023. The genera most frequently reported were Fusarium, followed by Penicillium, Aspergillus, and Alternaria. Among the published reports, the majority focused on the analysis of Deoxynivalenol (DON), which garnered twice as many reports compared to those focusing on Aflatoxins, Zearalenone, and Ochratoxin A. The concentration of these toxins, in most cases determined by HPLC-MS/MS or HPLC coupled with a fluorescence detector (FLD), was occasionally observed to exceed the maximum limits established by national and/or international authorities. The prevalence of mycotoxins in flour samples from the European Union (EU) and China, as well as in foods intended for infants, exhibited a significant reduction compared to other commercial flours assessed by a meta-analysis investigation.
