Category Archives: Microbiology

Research – Peroxyacetic Acid Effectiveness against Salmonella on Raw Poultry Parts is not Affected by Organic Matter

Journal of Food Protection

Organic matter (OM) accumulation is common in chill tanks used to decontaminate raw poultry parts during processing. Organic matter negatively affects the antimicrobial activity of chlorine-based compounds, but its effect on the antimicrobial effectiveness of peroxyacetic acid (PAA) on poultry meat has not been described. Therefore, this study evaluated the effect of OM on the efficacy of PAA solutions in simulated post-chill tanks to reduce Salmonella artificially inoculated onto chicken parts. Chicken thighs were inoculated with a five-strain cocktail of poultry-borne Salmonella enterica serovars at ca. 6 log10CFU/ml. Then, the thighs were immersed for 30 or 45 s in PAA solutions (500 or 1,000 ppm) with chicken slurry to simulate OM accumulation (0, 15 or 30 g/L). The thighs were rinsed with neutralizing buffered peptone water (100 ml) and rinsates were plated onto XLD agar. Experiments were performed in triplicate (3 thighs/treatment/replicate). Chemical oxygen demand (COD), total nitrogen (TN), and pH were measured as the water quality parameters of the PAA solutions before and after use. COD ranged from 2,905 mg/L in unused 500-ppm solutions without added OM to 6,290 mg/Lin used 1,000-ppm solutions with 30 g/L of OM. Initial TN was 42.5 ± 2.0 mg/L and 60.9 ± 8.3 mg/L for 15 and 30 g/L OM, which increased by 27 ± 17 mg/L after use. The pH of solutions ranged from 3.16 ± 0.14 to 3.42 ± 0.09 for the 1,000-ppm solutions and from 3.59 ± 0.06 to 3.96 ± 0.06 for the 500-ppm solutions. Mean Salmonella reductions were 0.9 ± 0.1 log10CFU/ml of rinsate for the 500-ppm PAA treatment and 1.1 ± 0.1 log10CFU/ml of rinsate for 1,000-ppm PAA treatment. Exposure time did not have a significant effect on the logarithmic reductions. There was no significant effect of OM concentration (p>0.05) on the reductions, indicating that the antimicrobial efficacy was not affected and that PAA solutions may continue to be reused as long as the PAA concentration is actively monitored.

Research – Investigating and Preventing Foodborne Outbreaks Linked to Raw Milk and Raw Milk Dairy Products

NY Food Safety

Foodborne disease outbreaks and cases linked to raw milk and raw milk dairy products continue to be a concern. Investigations of these outbreaks often represent unique challenges, such as patients who may not be willing to disclose the source of the raw milk or identifying appropriate samples to collect on potential source farms (do you know what “milk socks” are and where to find them?)

To learn more about raw milk and dairy products, how to investigate outbreaks linked to them, and how to help prevent these outbreaks, view the NY CoE webinar on “Investigating and preventing foodborne disease outbreaks linked to raw milk and raw milk dairy products”, presented by Dr. Martin Wiedmann from Cornell University on 6/2/2022. Following the presentation, a panel of individuals who have been involved with investigating raw milk outbreaks participated in a Q&A dialogue. Panelists included subject matter experts from the New York State Department of Health, New York State Department of Agriculture and Markets, Maine Department of Health and Human Services, and Pennsylvania Department of Public Health. The webinar was recorded and can be accessed on the NY CoE YouTube here.

Research – Dynamics of Salmonella enterica and antimicrobial resistance in the Brazilian poultry industry and global impacts on public health

PLOS One

Non-typhoidal Salmonella enterica is a common cause of diarrhoeal disease; in humans, consumption of contaminated poultry meat is believed to be a major source. Here we determine that Senterica serovars Heidelberg and Minnesota were the most prevalent serovars in Brazilian poultry and in poultry products imported from Brazil into the UK. However, long-term surveillance data collected by the UK Health Security Agency showed no increase in the incidence of Salmonella Heidelberg or Salmonella Minnesota in human cases of clinical disease in the UK following the increase of these two serovars in Brazilian poultry. Salmonella Heidelberg and Salmonella Minnesota clades shared resistance to sulphonamides, tetracyclines and beta-lactams conferred by sul2tetA and blaCMY-2 genes, not widely observed in other co-circulating serovars despite similar selection pressures. The sul2 and tetA genes were concomitantly carried on IncC plasmids, whereas blaCMY-2 was either co-located with the sul2 and tetA genes on IncC plasmids or independently on IncI1 plasmids. These findings suggest that introduction of the Salmonella Enteritidis vaccine, in addition to increasing antimicrobial use, could have resulted in replacement of salmonellae in Brazilian poultry flocks with serovars that are more drug resistant, but less associated with disease in humans in the UK.

RASFF Alerts – Animal Feed -Salmonella – Chicken by Products- Complete Feed for Dogs – Dried Horse Lung for Dogs

RASFF

Salmonella in chicken by-products from Belgium in the Netherlands

RASFF

Salmonella in complete feed for dogs from the Netherlands in Belgium

RASFF

Salmonella in dried horse lung for dogs from Austria in the Netherlands

Research – Dangerous pathogens lurk in wet areas

Science Daily

Hospitals and homes are havens for germs and disease — but a well-regulated hot water system can prevent the spread of waterborne pathogens, including an emerging infectious disease problem from ‘superbugs’ which can be resistant to most antibiotics.

However, Flinders University experts warn that some drinking water treatment methods may be ineffective against some waterborne pathogens that pose a threat to immune-compromised individuals — and point-of-use devices such as taps and showerheads may create havens for these microorganisms to grow.

The elderly, newborns and those with compromised immune systems are especially vulnerable to waterborne infections. Numbers are rising of people with conditions such as advanced age, cancer and immunodeficiency issues who may be more at risk of ‘opportunistic premise plumbing pathogens’ (OPPP) infection.

Regular changes to tap and outlet filters, hot water service maintenance checks (for heat and pressure) and effective cleaning of shower and tap faucets are recommended for immune-compromised patients receiving healthcare at home or post-surgical management.

Healthcare at home has emerged as an alternative to extensive inpatient hospital stays — especially through the COVID-19 pandemic, to reduce the burden on the healthcare system and to support those with potential long term respiratory side effects.

“These measures can help reduce the risk of waterborne infections in home care, along with quality treatments of mains water to provide safe drinking water,” says lead researcher Claire Hayward, from Flinders University’s College of Science and Engineering.

A new study published in the journal Current Opinion in Infectious Diseases, by environmental health experts at Flinders University, found evidence of hospital water as a source of potential infection and even antimicrobial and multidrug resistant organisms.

“Biofilms formed on taps, showers, drains and other outlets provide an ideal niche to harbour these dangerous antibiotic resistant pathogens originating from the supply water or the human microbiota from washing contaminated hands,” says Ms Hayward.

OPPP infections such as Legionella pneumophila, Pseudomonas aeruginosa and Mycobacterium avium are a group of waterborne pathogens that are slowly receiving increased public health attention in infection control guidelines.

“However, drinking water as a source of healthcare-associated infections continues to be overlooked or underestimated in this monitoring,” Ms Hayward says.

The OPPP waterborne pathogens can be disinfectant resistant and persist in refuges with low nutrients, then form biofilms capable of supporting other clinically relevant pathogens such as Staphylococcus aureus, Enterobacteriaceae, Klebsiella pneumoniae and Escherichia coli.  

Once these biofilms are established on plumbing surfaces, there are many ways for contamination and transmission. The design of outlet devices that result in splashing and aerosolising of water can also increase the risk of contaminating nearby areas.

“The rise of these resistant pathogens has been identified by the World Health Organization and US Centers for Disease Control and Prevention as one of the most significant threats to global public health,” says research co-author, Flinders University microbiology expert Professor Melissa Brown.

“While the COVID-19 pandemic has increased the use of disinfectants and sanitisers, particularly in healthcare facilities, these antiseptic soaps do not tackle what’s going on behind the scenes in the water supply pipes and faucets,” she says.

The researchers recommend broad, universal surveillance guidelines to understand the role of drinking water and water-related devices to reduce healthcare associated infections and the rise of possible antimicrobial resistance that poses a threat to at risk individuals in residential and healthcare settings.

Belgium – Belgium reports large rise in foodborne illness outbreaks in 2021

Food Safety News

An additional 200 outbreaks were recorded in Belgium in 2021 versus the year before, according to the Federal Agency for the Safety of the Food Chain (FASFC).

There was a significant increase in the number of food poisoning outbreaks from 333 in 2020 to 547 in 2021. The number of people affected in them went from 1,262 with 27 hospitalizations and two deaths to 2,070 sick, 78 hospitalizations but no deaths in 2021.

FASFC, also known as AFSCA and FAVV, said it was important to remember 2020 was an exceptional year because of the closure of many food businesses caused by COVID-19 related restrictions.

Figures for the past year are still slightly lower than 2019, when 571 outbreaks were recorded and 2,457 people were affected.

The agent was unknown in 512 outbreaks with almost 1,700 illnesses in 2021, according to data from Sciensano, the national public health institute.

Read more at the link above.

USA – FDA Core Investigation Table

FDA

What’s New

  • For the Salmonella Senftenberg outbreak in Peanut Butter (ref# 1067), the outbreak status has ended and the investigation status is now closed.
  • A new outbreak has been added to the table: Cyclospora in a not yet identified food (ref #1084).
  • For the Salmonella Paratyphi B var. L(+) tartrate+ outbreak (ref# 1072) in a not yet identified food, the outbreak status has ended. FDA’s investigation status is still ongoing.
Date
Posted
Ref Pathogen or
Cause of Illness

Product(s)
Linked to
Illnesses

(if any)
Total
Case Count

Status
7/27/

2022

1084 Cyclospora Not Yet Identified 51 Active
6/29/

2022

1076 Not Yet Identified Frozen Food See Outbreak
Advisory
Active
6/22/

2022

1075 Salmonella
Braenderup
Not Yet Identified 70 Active
6/22/

2022

1072 Salmonella
Paratyphi B var. L(+) tartrate+
Not Yet Identified 14 Active
6/15/

2022

1070 Listeria
monocytogenes
Not Yet Identified 12 Active
6/8/

2022

1068 E. coli
O157:H7
Not Identified 10 Closed
6/1/

2022

1066 Hepatitis A Virus Strawberries See Outbreak
Advisory
Active
5/25/

2022

1067 Salmonella 
Senftenberg
Peanut Butter See Outbreak Advisory Closed
4/20/

2022

1064 Not Yet
Identified
Dry Cereal 558 adverse
event reports
Active
4/13/

2022

1057 Listeria
monocytogenes
Ice Cream See Outbreak Advisory Active
3/30/

2022

1060 None
Identified
Meal Replacement
Drink
6 adverse
event reports
Closed
3/16/

2022

1055 Salmonella
Saintpaul
Not Identified 60 Closed
2/17/

2022

1056 Cronobacter
sakazakii
Powdered
Infant
Formula
See
Advisory
Active (IMG)
2/9/

2022

1040 Listeria
monocytogenes
Not Identified 20 Closed
2/2/

2022

1054 Enteroinvasive
E. coli
O143:H26
Not
Identified
16 Closed
1/10/

2022

1050 E. coli
O121:H19
Romaine 4 Closed

India – 40 students fall sick after mid-day meal in AP

New Indian Express

KURNOOL: As many as 40 students of a Mandal Parishad upper primary school fell sick after reportedly eating mid-day meal on Tuesday. The school is located at Chakrala village in Pathikonda mandal of Kurnool district.All the students were immediately admitted to the Pathikonda government hospital, where the condition of five was stated to be critical.

According to reports, the authorised agency served mid-day meal to the students as usual in the afternoon. Out of the total strength of 220, 160 students had their lunch at the school. Later, they developed stomach ache, vomiting and motions.

Samples of water and food were collected for lab testing.

Canada – Canada sets regulations for some romaine grown in certain parts of California

Food Safety News

The Canadian Food Inspection Agency (CFIA) has announced the fall 2022 import requirements for U.S. romaine lettuce with special requirements for certain lettuce produced in California’s Salinas Valley.

Whole-head romaine lettuce and products containing romaine lettuce such as bagged salads that were produced in the four Salinas Valley counties of Santa Cruz, Santa Clara, San Benito, and Monterey must “submit an attestation form and Certificates of Analysis for each shipment to demonstrate that the romaine lettuce does not contain detectable levels of E. coli O157:H7,” according to the Canadian Food Inspection Agency (CFIA).

Denmark – Denmark searches for source of Salmonella outbreak

Food Safety News

kswfoodworld salmonella

Danish officials are trying to find the source of a Salmonella outbreak that has affected 16 people.

The sick people have been infected with the same type of Salmonella Enteritidis, according to the Statens Serum Institut (SSI).

Patients fell ill between March 31 and June 23. They are 11 men and five women between the ages of 8 to 59 years old with a median of 28.