Category Archives: Microbiological Risk Assessment

Research – An outbreak of Cryptosporidium parvum linked to pasteurised milk from a vending machine in England: a descriptive study, March 2021

Posted on December 18, 2022 by | Leave a comment

Cambridge Org

We describe the investigations and management of a Cryptosporidium parvum outbreak of linked to consumption of pasteurised milk from a vending machine. Multiple locus variable number of tandem repeats analysis was newly used, confirming that C. parvum detected in human cases was indistinguishable from that in a calf on the farm. This strengthened the evidence for milk from an on-farm vending machine as the source of the outbreak because of post-pasteurisation contamination. Bacteriological indicators of post-pasteurisation contamination persisted after the initial hygiene improvement notice. We propose that on-farm milk vending machines may represent an emerging public health risk.

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Posted in Bacteria, bacterial contamination, Cryptosporidiosis, Cryptosporidium, Decontamination Microbial, food bourne outbreak, food contamination, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak

Research -Quantitative microbiological risk assessment of traditional food of animal origin produced in short supply chains in Poland

Posted on December 18, 2022 by | Leave a comment

EFSA

Abstract

Polish raw-milk cheeses produced in short supply chains may pose a threat to consumer safety due to pathogen presence. Listeria monocytogenes is a bacterium of great importance for the food safety of refrigerated RTE foods due to its ability to grow at refrigeration temperatures.

During the EU-FORA fellowship, a stochastic risk assessment was designed and executed to estimate the risk for consumers from L. monocytogenes in these products. The aim was to develop a probabilistic QMRA model that would incorporate the variability and uncertainty of the model’s inputs such as prevalence, initial concentration levels, product intrinsic factors, domestic storage temperature and consumer behaviour. The project involved data collection and analysis, growth model selection, mathematical modelling and Monte Carlo analysis in R programming language.

Microbiological and physicochemical testing were carried out throughout the year on two types of cheeses in combination with a domestic refrigerator temperature survey and accompanying consumption questionnaire. Collected data were fitted to probability distributions using R. The appropriate growth model for the pathogen was selected based on an inoculation study performed on one of the raw-milk cheeses and the chosen mathematical model was written into the R script developed for the QMRA. The dose–response model used the ingested dose calculated from the modelled concentration of L. monocytogenes at the time of consumption and the single serving size from the questionnaire to estimate the probability of illness. The final risk was expressed as probability of listeriosis for Polish consumers per serving of raw-milk cheese.

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Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Listeria, Listeria monocytogenes, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Raw Milk, raw milk cheese, Raw Milk Cream, Research

Research – Microbiological Quality of Pig Carcasses in a Slaughterhouse under Risk-Based Inspection System

Posted on December 17, 2022 by | Leave a comment

MDPI

Abstract

Meat product inspection is one of the procedures adopted more than a century ago to guarantee food quality and safety for consumption. Due to technology and regulation advancement for farming and slaughtering pigs, a change in zoonotic profile attributed to pork has been identified.
 
Thus, a global movement began to establish inspection parameters based on epidemiological risk profiles, culminating in the publication of a new regulation in Brazil in 2018. This normative instruction establishes that slaughterhouses under federal inspection must implement risk-based inspection until 2028.
 
Changes in the inspection system can generate questions and objections on the part of customers and consumer markets. In order to assess microbiological contamination when adopting a risk-based inspection system, the occurrence of Salmonella spp. and the quantification of Enterobacteriaceae and mesophilic aerobic counts were compared in pig carcasses slaughtered under traditional and risk-based inspection systems.
 
A statistical significance reduction was identified regarding the quantification of Enterobacteriaceae (log −0.18 to −1.61 CFU/cm2) and mesophilic aerobic counts (log 4.60 to 3.49 CFU/cm2). The occurrence of Salmonella spp. did not show a significant difference (4% to 5.3%). The results allowed us to conclude that adopting risk-based inspection systems improves food safety through Enterobacteriaceae and mesophilic aerobic counts reduction.

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Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Safety, Food Safety Management, food safety training, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk

Research – Prevalence ofE. coliO157:H7 in water sources: an overview on associated diseases, outbreaks and detection methods

Posted on December 17, 2022 by | Leave a comment

Elsevier

Escherichia coliO157:H7 is a zoonotic pathogen with its ability to cause human illness ranging from diarrheal dis-ease to fatal hemolytic uremic syndrome.E. coliO157:H7 had been associated with waterborne outbreaksresulting in high morbidity and mortality worldwide. Therefore, it is important to investigate the prevalence ofE. coliO157:H7 in water sources especially used for drinking and to develop the diagnostic methods for itsearly detection. The review describes traditional cultural methods, immunological techniques, and polymerasechain reaction (PCR)–based methods for detection of this bacterium in water sources. The current PCR-basedtechniques such as real-time PCR are more specific and sensitive and require less detection time (b3 hours).These methods can be applied for regular water monitoring and proper management of water sources to preventwaterborne diseases due toE. coliO157:H7.

 

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Posted in Decontamination Microbial, E.coli O157, E.coli O157:H7, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, STEC, STEC E.coli

Research – Vibrio vulnificus and Vibrio parahaemolyticus in Oysters under Low Tidal Range Conditions: Is Seawater Analysis Useful for Risk Assessment?

Posted on December 17, 2022 by | Leave a comment

MDPI

Food Illness

Abstract

Human-pathogenic Vibrio bacteria are acquired by oysters through filtering seawater, however, the relationships between levels of these bacteria in measured in oysters and overlying waters are inconsistent across regions. The reasons for these discrepancies are unclear hindering our ability to assess if -or when- seawater samples can be used as a proxy for oysters to assess risk. We investigated whether concentrations of total and human pathogenic Vibrio vulnificus (vvhA and pilF genes) and Vibrio parahaemolyticus (tlh, tdh and trh genes) measured in seawater reflect concentrations of these bacteria in oysters (Crassostrea virginica) cultured within the US lower Chesapeake Bay region. We measured Vibrio spp. concentrations using an MPN-qPCR approach and analyzed the data using structural equation modeling (SEM). We found seawater concentrations of these bacteria to predictably respond to temperature and salinity over chlorophyll a, pheophytin or turbidity. We also inferred from the SEM results that Vibrio concentrations in seawater strongly predict their respective concentrations in oysters. We hypothesize that such seawater-oyster coupling can be observed in regions of low tidal range. Due to the ease of sampling and processing of seawater samples compared to oyster samples, we suggest that under low tidal range conditions, seawater samples can foster increased spatial and temporal coverage and complement data associated with oyster samples.

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Posted in Contaminated water, Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Vibrio, Water, water microbiology, Water Safety

Research – EFSA – FOODSAFETY4EU: paving the way for the food safety system of the future

Posted on December 17, 2022 by | Leave a comment

EFSA

Abstract

Food safety is a top priority for the European Commission, which policies aim at sustaining a high level of protection of human health and consumers’ interests, while ensuring an effective functioning of the internal EU market. Under the New Transparency Regulation (EU/2019/138), the 3-year EU-fundedFOODSAFETY4EUproject (FS4EU) kicked off in January 2021, represents a significant step for the European Union (EU) food safety system (FSS), towards more transparency, better engagement, and closer cooperation.

This Horizon 2020 Project, coordinated by CNR-ISPA (Italy), focuses on building a multi-stakeholder platform for the future EU FSS. The FOODSAFETY4EU Network currently consists of 23 consortium partners and around 50 stakeholders: Food Safety Authorities (FSA), consumer associations, academia, research centres and networks, food industries and sector associations, thinktanks, etc. Through a structured, digitally supported, participatory process, the platform hosts the co-design of future strategic research and innovation agenda (SRIA), as well as risk communication models tailored to the specificities of various target groups. Among the goals: providing scientific advice and technical support for EU food safety policies, by enabling actors to access, share and exchange scientific knowledge, resources, and data more efficiently, to better synchronise food safety research and policies, and to contribute to a more transparent communication through the FSS.

Overall, the FS4EU project underpins the EFSA missions in risk assessment, including risk communication, with the ambition to be a basis for a Knowledge Centre for Food Safety in Europe. Accordingly, the EU-FORA 2021–2022 fellow (based in CNR-ISPA under the Work Programme RECIPE: Risk AssEssment/Risk CommunIcation: understanding the context and addressing Priorities of the futurE–a learning-by-doing approach) committed to multiple Work Packages, actively participated to various activities, thus successfully contributing to the FS4EU project meeting its objectives by the end of 2023

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Posted in Decontamination Microbial, EFSA, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Research

Research – Emerging health threat and cost of Fusarium mycotoxins in European wheat

Posted on December 16, 2022 by | Leave a comment

Nature.com

CDC Fusarium1

Mycotoxins harm human and livestock health, while damaging economies. Here we reveal the changing threat of Fusarium head blight (FHB) mycotoxins in European wheat, using data from the European Food Safety Agency and agribusiness (BIOMIN, World Mycotoxin Survey) for ten years (2010–2019). We show persistent, high, single- and multi-mycotoxin contamination alongside changing temporal-geographical distributions, indicative of altering FHB disease pressure and pathogen populations, highlighting the potential synergistic negative health consequences and economic cost.

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Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Fusarium Toxin, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, mold, Mold Toxin, Mould Toxin, Mould/Mold, Mycotoxin

RASFF Alerts – Animal Feed – Salmonella – Pet Food – Rapeseed Mealt

Posted on December 16, 2022 by | Leave a comment

RASFF

Salmonella Spp in processed animal proteins (SOA cat.3) for petfood from Czech Republic in Italy

RASFF

Salmonella Senftenberg in the post-extraction rapeseed meal from Ukraine in Poland

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Posted in Animal Feed, Animal Feed Salmonella, Animal Feed Testing, Decontamination Microbial, Food Micro Blog, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Pet Food, Pet Food Salmonella, Pet Food Testing, RASFF, Salmonella

USA -39 California, Arizona, Texas and Illinois Salmonella illnesses linked to raw fish

Posted on December 15, 2022 by | Leave a comment

Food Poison Journal

A total of 39 people infected with the outbreak strain of Salmonella Litchfield were reported from 4 states – California, Arizona, Texas and Illinois.

 

 

 

 

 

Illnesses started on dates ranging from June 14, 2022, to October 23, 2022 (see timeline). Sick people ranged in age from 1 to 80 years, with a median age of 33, and 66% were female. Of 32 people with information available, 15 were hospitalized. No deaths were reported.

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Posted in Decontamination Microbial, food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak, Salmonella

Estonia improves fish controls after Listeria outbreak

Posted on December 15, 2022 by | Leave a comment

Food Safety News

Measures taken by Estonian authorities following a Listeria outbreak have improved the fish control system, according to the European Commission.

A remote DG Sante audit, in October 2020, looked at checks to ensure smoked fish meets microbiological food safety criteria, as well as measures taken by authorities in non-compliances.

Eight Rapid Alert System for Food and Feed (RASFF) reports for Listeria monocytogenes in smoked fishery products from Estonia have been issued since 2015. All but one concerned the same establishment.

A multi-country outbreak of 22 listeriosis cases linked to cold-smoked salmon and trout pointed toward M.V Wool, an Estonian processing company as the manufacturer of these fish products. This was based on traceability information and a match between the outbreak strain and samples on the processing line and in four batches of the final product. Five countries were affected from 2014 to 2019 and five people died.

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Posted in Death, Decontamination Microbial, food bourne outbreak, food death, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Poisoning Death, Foodborne Illness, Foodborne Illness Death, foodborne outbreak, foodbourne outbreak, Illness, Listeria, Listeria monocytogenes, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak