Category Archives: Microbiological Risk Assessment

Research – Comparative efficiency of carbohydrates on the biofilm-forming ability of enteroaggregative Escherichia coli

Wiley Online

The biofilm-forming ability of microbes is often correlated with drug resistance; however, this association among enteroaggregative Escherichia coli (EAEC) strains remains naïve. Moreover, the investigations exploring uptake and assimilation of sugars either qualitatively or quantitatively by the biofilm-forming EAEC strains have not yet been documented. Hence, this study investigated the role of various categories of sugars (monosaccharides, disaccharides, and polysaccharide) on the biofilm-forming ability of three each of multi-drug-resistant (MDR) as well as non-MDR typical EAEC strains. The EAEC strains exhibited black colonies on congo red agar (CRA) with monosaccharides and disaccharides, whereas all the strains exhibited red colonies on CRA with the polysaccharide. Although significant (p < 0.001) biofilm-forming ability was observed by all the EAEC strains in sugar-dependent microtiter plate assay, color change from pink to orange and yellow was noticed in presence of monosaccharides and disaccharide, while absent in polysaccharides. Besides, significantly higher bacterial counts were observed in presence of monosaccharides and disaccharides (p < 0.01); however, such significant bacterial counts were not noticed with the polysaccharide. Further, the real-time PCR data exhibited comparatively higher relative expression of biofilm-associated fis gene among EAEC strains grown in presence of monosaccharides, followed by disaccharide and polysaccharide, respectively that concurred with the flow cytometry data. No significant (p > 0.05) difference was observed in the biofilm-forming ability between MDR- and non-MDR EAEC strains. This study illustrated the role of carbohydrates on the biofilm-forming ability of EAEC and warranted utmost attention concerning the usage of carbohydrates in food processing industries, for its incorporation as functional food additives.

Research – Effect of hydrophobic/hydrophilic characteristics of B. cereus spores on their resistance to detergents

Journal of Food Protection

Bacillus cereus spores have the ability to adhere to solid surfaces, including stainless steel, a material widely used in food industries. Adhesion of spores allows for recontamination during food processing, and the cleaning and disinfection are largely used by industries to control them. Hence, this study aims to assess the detachment capacity (or removing activity) of sodium hydroxide, nitric acid, phosphoric acid-based detergent, chlorine-based detergent; against two adhered Bacillus cereus spores (hydrophobic strain and hydrophilic strain) to stainless steel surfaces. Microorganism adhesion on the surfaces reached 5.5 log CFU/cm² the two strain studied. Two protocols composed of combinations of chemical compounds concentration, temperature, and contact time were tested. The inactivation kinetics shapes were convex and were modelled by the Weibull model. The effect of temperature and biocide concentration were quantified using a Bigelow like model. The temperature applied during of treatment of the cleaning in place is an important factor acting on the speed of inactivation or detachment of Bacillus cereus spores. However, this efficiency depends on the hydrophobic characteristics of Bacillus cereus spores. The concentration of detergent and acid also affects the inactivation rate. The inactivation rate, whereas the character of hydrophobic does not intervene for the chlorine alkaline treatments.

Research – Multistate Outbreak Investigation of Salmonella Infections Linked to Kratom: A Focus on Traceback, Laboratory, and Regulatory Activities

Journal of Food Protection

kswfoodworld.com

During the spring of 2018, the U.S. Food and Drug Administration (FDA), U.S. Centers for Disease Control and Prevention (CDC), states and local public health agencies responded to a multistate outbreak of gastrointestinal illnesses caused by multiple   Salmonella   serovars and associated with consumption of kratom, a product harvested from a tropical tree native to Southeast Asia. The outbreak included 199 case-patients reported by 41 U.S. states, with illness onset dates ranging from January 11, 2017 to May 8, 2018, leading to 54 hospitalizations, and no deaths. Case-patients reported purchasing kratom products from physical and online retail points of service (POS). Products distributed to 16 POS where 24 case-patients from 17 states purchased kratom were selected for traceback investigation. Traceback revealed that the kratom was imported from several countries, the most common being Indonesia. Local and state officials collected product samples from case-patients and retail POS. The FDA collected 76 product samples from POS and distributors, of which 42 (55%) tested positive for   Salmonella  . The positive samples exhibited a wide range of pulsed field gel electrophoresis (PFGE) patterns and whole genome sequence (WGS) genetic heterogeneity, and a total 25 of 42 (60%) yielded at least one isolate indistinguishable from one or more outbreak-related clinical isolates. While it does not exclude a possibility of a single contamination source, the extent of genetic diversity exhibited by the   Salmonella   isolates recovered from product samples and a lack of traceback convergence, suggested that kratom was widely contaminated across multiple sites from which it was grown, harvested, and packaged. As a result of the contamination, kratom products were recalled by numerous firms (both voluntarily and mandatory). Epidemiologic, traceback, and laboratory evidence supported the conclusion that kratom products were associated with illnesses.

India – 15 kids suffer ‘food poisoning’ in Andhra, adulterated food at Anganwadi

New Indian Express

NELLORE: At least 15 children fell sick after they reportedly ate adulterated food at an Anganwadi in Podalakuru town of Nellore district. According to reports, the children were suffering from diarrhoea since Wednesday evening. Parents staged a demonstration at the Anganwadi and blamed the negligent attitude of the staff for delaying treatment to their children.

They alleged that the children began vomiting and fell sick after eating food provided at the Anganwadi. They said the children were admitted to a local hospital for treatment where doctors confirmed that they fell sick due to the adulterated food they ate. The children are getting treated at various hospitals in Podalakuru and Nellore city. Sources said their condition is stable now.

USA – Kick off your Super Bowl gathering with critical food safety precautions

Food Safety News

Sport football.svg

Here are some food safety tips for Super Bowl Sunday from the USDA:

#1 Remember Your Four Steps to Food Safety

  • Clean: Wash hands for 20 seconds before and after handling food, especially raw meat and poultry. Clean hands, surfaces and utensils with soap and warm water before cooking, during preparation and serving. After cleaning surfaces that raw meat and poultry have touched, apply a commercial or homemade sanitizing solution (1 tablespoon of liquid chlorine bleach per gallon of water). Use hand sanitizer that contains at least 60 percent alcohol.
  • Separate: Use separate cutting boards, plates and utensils to avoid cross-contamination between raw meat or poultry and foods that are ready-to-eat, such as raw vegetables and fruits.
  • Cook: Confirm foods are cooked to a safe internal temperature by using a food thermometer.
  • Chill: Chill foods promptly if not consuming immediately after cooking. Don’t leave food at room temperature for longer than two hours. Set out portions of foods and refill serving dishes instead of setting out all of the food at the beginning of the game.

#2 Cook Your Food to a Safe Internal Temperature

  • Using a food thermometer, ensure you reach a safe internal temperature when cooking: meat (whole beef, pork and lamb) 145 degrees F with a 3-minute resting time after removing from heat; ground meats 160 degrees F; poultry (ground and whole) 165 degrees F; eggs 160 degrees F; fish and shellfish 145 degrees F; and leftovers and casseroles 165 degrees F.
  • If chicken wings are on the menu, use a food thermometer on several wings to gauge the doneness of the entire batch. If one is under 165 F, continue cooking all wings until they reach that safe internal temperature.

#3 Avoid the Danger Zone

  • Bacteria multiply rapidly between 40 degrees F and 140 degrees F. This temperature range is called the Danger Zone.
  • Perishable foods, such as chicken wings, deli wraps and meatball appetizers, should be discarded if left out for longer than two hours. To prevent food waste, refrigerate or freeze perishable items within two hours.
    • Keep cold foods at a temperature of 40 degrees F or below by keeping food nestled in ice bowls or refrigerated until ready to serve.
    • Keep hot foods at a temperature of 140 degrees F or above by placing food in a preheated oven, warming trays, chafing dishes or slow cookers.
  • Divide leftovers into small portions and refrigerate or freeze them in shallow containers, which helps leftovers cool quicker than storing them in large containers.

#4 Keep Takeout Food Safe

  • If you order food and it’s delivered or picked up in advance of the big game, divide the food into smaller portions or pieces, place in shallow containers and refrigerate until ready to reheat and serve. You can also keep the food warm (above 140 degrees F) in a preheated oven, warming tray, chafing dish or slow cooker.
  • When reheating food containing meat or poultry, make sure the internal temperature reaches 165 degrees F as measured by a food thermometer.
  • If heating food in the microwave, ensure that contents are evenly dispersed. Because microwaved food can have cold spots, be sure to stir food evenly until the food has reached a safe internal temperature throughout.

A special word on salsa and guacamole:

“The reason that salsa and guacamole are so susceptible to contamination is that they are made with multiple raw, uncooked vegetables and are often stored at room temperature,” according to the Food and Drug Administration.

In addition to being left out for long periods of time, salsa and guacamole often contain diced raw produce including hot peppers, tomatoes and cilantro, increasing their chance of carrying harmful bacteria.

Anyone preparing fresh salsa and guacamole at home should be aware that these foods contain raw ingredients and that they should be carefully prepared and refrigerated to help prevent illness.

To prevent bacteria growth, these side dishes should be refrigerated within two hours. Those serving these foods should be aware of the length of time they have been out at room temperature.

Research – Denmark – New Methods to Reduce Campylobacter on Chicken Meat

Food DTU

Campylobacter kswfoodworld

Researchers and businesses will use a GUDP grant to develop new ways to prevent campylobacter bacteria from ending up on chicken meat and to ensure that fewer consumers get sick.

The greater focus on more sustainable diets may increase demand for chicken, which is the meat with the lowest carbon footprint. However, chicken is also the food source that most frequently gives Danes and Europeans campylobacter infections, which is a major challenge for the food industry.

Because chicken may become an even more popular food in future, it is important to gain more knowledge about and develop more solutions for reducing the presence of campylobacter bacteria in broiler chickens. By 2025, chicken producers will also have to comply with stricter EU rules on preventing campylobacter in chicken meat.

Moreover, free-range flocks of broiler chickens are far more often campylobacter-positive than conventional flocks. This is because they roam outside, where the bacterium occurs naturally. Existing measures used for conventional broilers such as increased biosecurity and fly nets are therefore not practical for free-range and organic broiler chickens.

Promising methods to be tested on a larger scale

In the SafeChicken project, researchers from DTU National Food Institute and DTU’s Department of Chemical Engineering will work with the Danish chicken producer Danpo and the Icelandic company Thor Ice Chilling Solutions to develop and test ways of producing chicken meat containing fewer campylobacter bacteria.

The project will test methods in different parts of the food chain in the production of both organic and conventional broiler chickens by: adding selected substances to the chickens’ feed and water which have the potential to prevent the growth of campylobacter bacteria in the chickens; reducing the prevalence of the bacteria in the environment with a new decontamination technology; and reducing bacteria on the carcasses by using a special cooling technology.

Some of the methods have already been tested and have shown promising results on a small scale under controlled conditions. To ensure that they are applicable in practice, also for free range and organic chicken production, the project partners will investigate and document the methods’ effectiveness under normal production conditions. They will also assess the extent to which each measure can help lower the risk of humans becoming infected and sick from campylobacter bacteria.

Combatting campylobacter from a ‘green’ perspective

The fewer bacteria chicken meat contains, the longer the meat can stay fresh. This will also result in fewer withdrawals of goods that have to be destroyed due to unacceptable levels of campylobacter. This will help to achieve the UN Sustainable Goal no. 12 concerning responsible consumption and production.

As part of the Green Development and Demonstration Programme (GUDP), the Danish Ministry of Food, Agriculture, and Fisheries has allocated DKK 7.4 million for the three-year project, which is led by DTU National Food Institute.

About campylobacter

Campylobacter infection is the most frequently occurring foodborne disease in the EU and Denmark. According to the official statistics, the bacterium makes approximately 4,500 Danes ill each year. However, many cases are never reported, and researchers believe the real number is about ten times higher.

The more campylobacter bacteria a food contains, the greater the risk that it will make people ill when they ingest it. Therefore, Danish authorities have initiated national action plans with the aim of reducing the risk of contracting campylobacter infection. The target for 2018-21 was a 50% reduction compared to 2013—and this goal has not yet been reached.

RASFF Alert – Animal Feed – Enterobacteriaceae

RASFF

Enterobacteriaceae in dog chews from Poland in France and Belgium

RASFF Alerts – Animal Feed -Salmonella – Sunflower Seed Cake – Rapeseed Meal – Rabbit Meal

RASFF

Salmonella Senftenberg in sunflower seed cake from Croatia in Poland, Belgium and the Netherlands

RASFF

Salmonella Livingstone in rapeseed meal from Germany in Switzerland

RASFF

Salmonella in rabbit meal from the Netherlands in the Czech Republic and Austria

Research – Characterization of Microbiological Quality of Whole and Gutted Baltic Herring

MDPI

There is growing interest in Baltic herring (Clupea harengus membras) and other undervalued, small-sized fish species for human consumption. Gutting or filleting of small-sized fish is impractical; hence, the aim of this study was to explore the suitability of the whole (ungutted) herring for food use. The microbiological quality of commercially fished whole and gutted herring was analysed with culture-dependent methods combined with identification of bacterial isolates with MALDI-TOF Mass Spectrometry and culture-independent 16S rRNA gene amplicon sequencing. Whole and gutted herring had between 2.8 and 5.3 log10 CFU g−1 aerobic mesophilic and psychrotrophic bacteria and between 2.2 and 5.6 log10 CFU g−1 H₂S-producing bacteria. Enterobacteria counts remained low in all the analysed herring batches. The herring microbiota largely comprised the phyla Proteobacteria, Firmicutes, and Actinobacteria (71.7% to 95.0%). ShewanellaPseudomonas, and Aeromonas were the most frequently isolated genera among the viable population; however, with the culture-independent approach, Shewanella followed by Psychrobacter were the most abundant genera. In some samples, a high relative abundance of the phylum Epsilonbacteraeota, represented by the genus Arcobacter, was detected. This study reports the bacterial diversity present in Baltic herring and shows that the microbiological quality was acceptable in all the analysed fish batches. View Full-Text

USA – FDA Core Outbreak Table Update – New Listeria monocytogenes Outbreak

FDA

Date
Posted

Ref

Pathogen
 

Product(s)
Linked to
Illnesses

(if any)

Investigation
Status

2/9/

2022

1040

Listeria
monocytogenes

Not Yet Identified

Active

2/2/

2022

1054

Enteroinvasive E. coli
O143:H26

Not Yet Identified

Active

1/10/

2022

1050

E. coli
O121:H19

Romaine

Closed

12/29/

2021

1052

E. coli
O157:H7

Packaged Salad

Active

12/20/

2021

1039

Listeria
monocytogenes

Packaged Salad

Active

12/15/

2021

1048

Listeria
monocytogenes

Packaged Salad

Active