Category Archives: Food Micro Blog

Research – Bacterial Antagonistic Species of the Pathogenic Genus Legionella Isolated from Cooling Tower

MDPI

Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe pneumonia. Cooling towers are a major source of large outbreaks of the disease. The growth of L. pneumophila in these habitats is influenced by the resident microbiota. Consequently, the aim of this study was to isolate and characterize bacterial species from cooling towers capable of inhibiting several strains of L. pneumophila and one strain of L. quinlivanii. Two cooling towers were sampled to isolate inhibiting bacterial species. Seven inhibitory isolates were isolated, through serial dilution plating and streaking on agar plates, belonging to seven distinct species. The genomes of these isolates were sequenced to identify potential genetic elements that could explain the inhibitory effect. The results showed that the bacterial isolates were taxonomically diverse and that one of the isolates may be a novel species. Genome analysis showed a high diversity of antimicrobial gene products identified in the genomes of the bacterial isolates. Finally, testing different strains of Legionella demonstrated varying degrees of susceptibility to the antimicrobial activity of the antagonistic species. This may be due to genetic variability between the Legionella strains. The results demonstrate that though cooling towers are breeding grounds for L. pneumophila, the bacteria must contend with various antagonistic species. Potentially, these species could be used to create an inhospitable environment for L. pneumophila, and thus decrease the probability of outbreaks occurring. View Full-Text

Research – Comparative efficiency of carbohydrates on the biofilm-forming ability of enteroaggregative Escherichia coli

Wiley Online

The biofilm-forming ability of microbes is often correlated with drug resistance; however, this association among enteroaggregative Escherichia coli (EAEC) strains remains naïve. Moreover, the investigations exploring uptake and assimilation of sugars either qualitatively or quantitatively by the biofilm-forming EAEC strains have not yet been documented. Hence, this study investigated the role of various categories of sugars (monosaccharides, disaccharides, and polysaccharide) on the biofilm-forming ability of three each of multi-drug-resistant (MDR) as well as non-MDR typical EAEC strains. The EAEC strains exhibited black colonies on congo red agar (CRA) with monosaccharides and disaccharides, whereas all the strains exhibited red colonies on CRA with the polysaccharide. Although significant (p < 0.001) biofilm-forming ability was observed by all the EAEC strains in sugar-dependent microtiter plate assay, color change from pink to orange and yellow was noticed in presence of monosaccharides and disaccharide, while absent in polysaccharides. Besides, significantly higher bacterial counts were observed in presence of monosaccharides and disaccharides (p < 0.01); however, such significant bacterial counts were not noticed with the polysaccharide. Further, the real-time PCR data exhibited comparatively higher relative expression of biofilm-associated fis gene among EAEC strains grown in presence of monosaccharides, followed by disaccharide and polysaccharide, respectively that concurred with the flow cytometry data. No significant (p > 0.05) difference was observed in the biofilm-forming ability between MDR- and non-MDR EAEC strains. This study illustrated the role of carbohydrates on the biofilm-forming ability of EAEC and warranted utmost attention concerning the usage of carbohydrates in food processing industries, for its incorporation as functional food additives.

Research – Effect of hydrophobic/hydrophilic characteristics of B. cereus spores on their resistance to detergents

Journal of Food Protection

Bacillus cereus spores have the ability to adhere to solid surfaces, including stainless steel, a material widely used in food industries. Adhesion of spores allows for recontamination during food processing, and the cleaning and disinfection are largely used by industries to control them. Hence, this study aims to assess the detachment capacity (or removing activity) of sodium hydroxide, nitric acid, phosphoric acid-based detergent, chlorine-based detergent; against two adhered Bacillus cereus spores (hydrophobic strain and hydrophilic strain) to stainless steel surfaces. Microorganism adhesion on the surfaces reached 5.5 log CFU/cm² the two strain studied. Two protocols composed of combinations of chemical compounds concentration, temperature, and contact time were tested. The inactivation kinetics shapes were convex and were modelled by the Weibull model. The effect of temperature and biocide concentration were quantified using a Bigelow like model. The temperature applied during of treatment of the cleaning in place is an important factor acting on the speed of inactivation or detachment of Bacillus cereus spores. However, this efficiency depends on the hydrophobic characteristics of Bacillus cereus spores. The concentration of detergent and acid also affects the inactivation rate. The inactivation rate, whereas the character of hydrophobic does not intervene for the chlorine alkaline treatments.

Research – Multistate Outbreak Investigation of Salmonella Infections Linked to Kratom: A Focus on Traceback, Laboratory, and Regulatory Activities

Journal of Food Protection

kswfoodworld.com

During the spring of 2018, the U.S. Food and Drug Administration (FDA), U.S. Centers for Disease Control and Prevention (CDC), states and local public health agencies responded to a multistate outbreak of gastrointestinal illnesses caused by multiple   Salmonella   serovars and associated with consumption of kratom, a product harvested from a tropical tree native to Southeast Asia. The outbreak included 199 case-patients reported by 41 U.S. states, with illness onset dates ranging from January 11, 2017 to May 8, 2018, leading to 54 hospitalizations, and no deaths. Case-patients reported purchasing kratom products from physical and online retail points of service (POS). Products distributed to 16 POS where 24 case-patients from 17 states purchased kratom were selected for traceback investigation. Traceback revealed that the kratom was imported from several countries, the most common being Indonesia. Local and state officials collected product samples from case-patients and retail POS. The FDA collected 76 product samples from POS and distributors, of which 42 (55%) tested positive for   Salmonella  . The positive samples exhibited a wide range of pulsed field gel electrophoresis (PFGE) patterns and whole genome sequence (WGS) genetic heterogeneity, and a total 25 of 42 (60%) yielded at least one isolate indistinguishable from one or more outbreak-related clinical isolates. While it does not exclude a possibility of a single contamination source, the extent of genetic diversity exhibited by the   Salmonella   isolates recovered from product samples and a lack of traceback convergence, suggested that kratom was widely contaminated across multiple sites from which it was grown, harvested, and packaged. As a result of the contamination, kratom products were recalled by numerous firms (both voluntarily and mandatory). Epidemiologic, traceback, and laboratory evidence supported the conclusion that kratom products were associated with illnesses.

India – 15 kids suffer ‘food poisoning’ in Andhra, adulterated food at Anganwadi

New Indian Express

NELLORE: At least 15 children fell sick after they reportedly ate adulterated food at an Anganwadi in Podalakuru town of Nellore district. According to reports, the children were suffering from diarrhoea since Wednesday evening. Parents staged a demonstration at the Anganwadi and blamed the negligent attitude of the staff for delaying treatment to their children.

They alleged that the children began vomiting and fell sick after eating food provided at the Anganwadi. They said the children were admitted to a local hospital for treatment where doctors confirmed that they fell sick due to the adulterated food they ate. The children are getting treated at various hospitals in Podalakuru and Nellore city. Sources said their condition is stable now.

Research – Escherichia coli O103 outbreak associated with minced celery among hospitalized individuals in Victoria, British Columbia, 2021

PHAC

Abstract

Background: In April 2021, a Shiga toxin-producing Escherichia coli (E. coli) (STEC) O103 outbreak was identified among patients at two hospitals in Victoria, British Columbia (BC). The objective of this study is to describe this outbreak investigation and identify issues of food safety for high-risk products prepared for vulnerable populations.

Methods: Confirmed cases of E. coli O103 were reported to the Island Health communicable disease unit. The provincial public health laboratory conducted whole genome sequencing on confirmed case isolates, as per routine practice for STEC in BC. Exposure information was obtained through case interviews and review of hospital menus. Federal and local public health authorities conducted an inspection of the processing plant for the suspect source.

Results: Six confirmed cases of E. coli O103 were identified, all related by whole genome sequencing. The majority of cases were female (67%) and the median age was 61 years (range 24–87 years). All confirmed cases were inpatients or outpatients at two hospitals and were exposed to raw minced celery within prepared sandwiches provided by hospital food services. A local processor supplied the minced celery exclusively to the two hospitals. Testing of product at the processor was infrequent, and chlorine rinse occurred before mincing. The spread of residual E. coli contamination through the mincing process, in addition to temperature abuse at the hospitals, are thought to have contributed to this outbreak.

Conclusion: Raw vegetables, such as celery, are a potential source of STEC and present a risk to vulnerable populations. Recommendations from this outbreak include more frequent testing at the processor, a review of the chlorination and mincing process and a review of hospital food services practices to mitigate temperature abuse.

USA – Kick off your Super Bowl gathering with critical food safety precautions

Food Safety News

Sport football.svg

Here are some food safety tips for Super Bowl Sunday from the USDA:

#1 Remember Your Four Steps to Food Safety

  • Clean: Wash hands for 20 seconds before and after handling food, especially raw meat and poultry. Clean hands, surfaces and utensils with soap and warm water before cooking, during preparation and serving. After cleaning surfaces that raw meat and poultry have touched, apply a commercial or homemade sanitizing solution (1 tablespoon of liquid chlorine bleach per gallon of water). Use hand sanitizer that contains at least 60 percent alcohol.
  • Separate: Use separate cutting boards, plates and utensils to avoid cross-contamination between raw meat or poultry and foods that are ready-to-eat, such as raw vegetables and fruits.
  • Cook: Confirm foods are cooked to a safe internal temperature by using a food thermometer.
  • Chill: Chill foods promptly if not consuming immediately after cooking. Don’t leave food at room temperature for longer than two hours. Set out portions of foods and refill serving dishes instead of setting out all of the food at the beginning of the game.

#2 Cook Your Food to a Safe Internal Temperature

  • Using a food thermometer, ensure you reach a safe internal temperature when cooking: meat (whole beef, pork and lamb) 145 degrees F with a 3-minute resting time after removing from heat; ground meats 160 degrees F; poultry (ground and whole) 165 degrees F; eggs 160 degrees F; fish and shellfish 145 degrees F; and leftovers and casseroles 165 degrees F.
  • If chicken wings are on the menu, use a food thermometer on several wings to gauge the doneness of the entire batch. If one is under 165 F, continue cooking all wings until they reach that safe internal temperature.

#3 Avoid the Danger Zone

  • Bacteria multiply rapidly between 40 degrees F and 140 degrees F. This temperature range is called the Danger Zone.
  • Perishable foods, such as chicken wings, deli wraps and meatball appetizers, should be discarded if left out for longer than two hours. To prevent food waste, refrigerate or freeze perishable items within two hours.
    • Keep cold foods at a temperature of 40 degrees F or below by keeping food nestled in ice bowls or refrigerated until ready to serve.
    • Keep hot foods at a temperature of 140 degrees F or above by placing food in a preheated oven, warming trays, chafing dishes or slow cookers.
  • Divide leftovers into small portions and refrigerate or freeze them in shallow containers, which helps leftovers cool quicker than storing them in large containers.

#4 Keep Takeout Food Safe

  • If you order food and it’s delivered or picked up in advance of the big game, divide the food into smaller portions or pieces, place in shallow containers and refrigerate until ready to reheat and serve. You can also keep the food warm (above 140 degrees F) in a preheated oven, warming tray, chafing dish or slow cooker.
  • When reheating food containing meat or poultry, make sure the internal temperature reaches 165 degrees F as measured by a food thermometer.
  • If heating food in the microwave, ensure that contents are evenly dispersed. Because microwaved food can have cold spots, be sure to stir food evenly until the food has reached a safe internal temperature throughout.

A special word on salsa and guacamole:

“The reason that salsa and guacamole are so susceptible to contamination is that they are made with multiple raw, uncooked vegetables and are often stored at room temperature,” according to the Food and Drug Administration.

In addition to being left out for long periods of time, salsa and guacamole often contain diced raw produce including hot peppers, tomatoes and cilantro, increasing their chance of carrying harmful bacteria.

Anyone preparing fresh salsa and guacamole at home should be aware that these foods contain raw ingredients and that they should be carefully prepared and refrigerated to help prevent illness.

To prevent bacteria growth, these side dishes should be refrigerated within two hours. Those serving these foods should be aware of the length of time they have been out at room temperature.

Belgium – 4 Albert Heijn brand products containing pumpkin seeds – Salmonella

AFSCA

Recall of Albert Heijn
Products: 4 Albert Heijn brand products containing pumpkin seeds.
Problem: possible presence of Salmonella.
In agreement with the FASFC, Albert Heijn is withdrawing four AH products containing pumpkin seeds from sale and reminding consumers of them due to the possible presence of salmonella.

Consumption of these products can potentially cause foodborne infections and pose a health risk, especially to young children, the elderly, people with weakened immune systems and pregnant women.

Albert Heijn asks his customers not to consume these products and to return them to the point of sale. There they will be replaced or refunded. Please contact a doctor in case of health problems.

Description of products:

• Product category: nuts and seeds
• Product name: AH pumpkin seed products
• Brand: Albert Heijn
• Best before date (BDD – date of minimum durability):
            o BDD 27/2/22 – AH ontbijt moerbei goji bessen mix
            o BDD 27/2 /22 – AH pitten mix salad
            o DDM 2/27/22 – AH Italiaanse mix
            o DDM 1/30/22 – AH pitten mix croutons
               (our packaging says “Look, smell and taste after this date”).
• Sales period: 9/15/21 – 11/30/21
• Type of packaging: jars/plastic bags
• Weight: various weights

France – ROCAMADOUR AOP 105G (3X35G) MONOPRIX GOURMET -Salmonella

Gov france

Identification information of the recalled product

  • Product category Food
  • Product subcategory Milk and dairy products
  • Product brand name MONOPRIX GOURMET
  • Model names or references ROCAMADOUR AOP 105G (3X35G) MONOPRIX GOURMET
  • Identification of products
    GTIN Lot Dated
    3350030108557 022 Date of minimum durability 07/03/2022
    3350030108557 026 Date of minimum durability 03/11/2022
  • Packaging Wrapped wooden tray of 3 cheeses
  • Marketing start/end date From 02/02/2022 to 10/02/2022
  • Storage temperature Product to be stored in the refrigerator
  • Health mark EN 46.204.002 EC
  • Geographic area of ​​sale Whole France
  • Distributors MONO PRICE

Practical information regarding the recall

  • Reason for recall Presence of Salmonella spp
  • Risks incurred by the consumer Salmonella spp (causative agent of salmonellosis)

Research – Denmark – New Methods to Reduce Campylobacter on Chicken Meat

Food DTU

Campylobacter kswfoodworld

Researchers and businesses will use a GUDP grant to develop new ways to prevent campylobacter bacteria from ending up on chicken meat and to ensure that fewer consumers get sick.

The greater focus on more sustainable diets may increase demand for chicken, which is the meat with the lowest carbon footprint. However, chicken is also the food source that most frequently gives Danes and Europeans campylobacter infections, which is a major challenge for the food industry.

Because chicken may become an even more popular food in future, it is important to gain more knowledge about and develop more solutions for reducing the presence of campylobacter bacteria in broiler chickens. By 2025, chicken producers will also have to comply with stricter EU rules on preventing campylobacter in chicken meat.

Moreover, free-range flocks of broiler chickens are far more often campylobacter-positive than conventional flocks. This is because they roam outside, where the bacterium occurs naturally. Existing measures used for conventional broilers such as increased biosecurity and fly nets are therefore not practical for free-range and organic broiler chickens.

Promising methods to be tested on a larger scale

In the SafeChicken project, researchers from DTU National Food Institute and DTU’s Department of Chemical Engineering will work with the Danish chicken producer Danpo and the Icelandic company Thor Ice Chilling Solutions to develop and test ways of producing chicken meat containing fewer campylobacter bacteria.

The project will test methods in different parts of the food chain in the production of both organic and conventional broiler chickens by: adding selected substances to the chickens’ feed and water which have the potential to prevent the growth of campylobacter bacteria in the chickens; reducing the prevalence of the bacteria in the environment with a new decontamination technology; and reducing bacteria on the carcasses by using a special cooling technology.

Some of the methods have already been tested and have shown promising results on a small scale under controlled conditions. To ensure that they are applicable in practice, also for free range and organic chicken production, the project partners will investigate and document the methods’ effectiveness under normal production conditions. They will also assess the extent to which each measure can help lower the risk of humans becoming infected and sick from campylobacter bacteria.

Combatting campylobacter from a ‘green’ perspective

The fewer bacteria chicken meat contains, the longer the meat can stay fresh. This will also result in fewer withdrawals of goods that have to be destroyed due to unacceptable levels of campylobacter. This will help to achieve the UN Sustainable Goal no. 12 concerning responsible consumption and production.

As part of the Green Development and Demonstration Programme (GUDP), the Danish Ministry of Food, Agriculture, and Fisheries has allocated DKK 7.4 million for the three-year project, which is led by DTU National Food Institute.

About campylobacter

Campylobacter infection is the most frequently occurring foodborne disease in the EU and Denmark. According to the official statistics, the bacterium makes approximately 4,500 Danes ill each year. However, many cases are never reported, and researchers believe the real number is about ten times higher.

The more campylobacter bacteria a food contains, the greater the risk that it will make people ill when they ingest it. Therefore, Danish authorities have initiated national action plans with the aim of reducing the risk of contracting campylobacter infection. The target for 2018-21 was a 50% reduction compared to 2013—and this goal has not yet been reached.