Category Archives: eae

Germany – Reblochon fermier Reblochon Excellence farm production – STEC E.coli

Posted on August 23, 2021 by | Leave a comment

LMW

Warning type: Food
Date of first publication: 08/20/2021
Product name:

Reblochon fermier Reblochon Excellence farm production

Manufacturer (distributor): Coopérative de Thônes France
Reason for warning:

Suspected contamination with undesirable bacteria (Escherichia coli Stec (gen stx and eae).

Packaging Unit: Counter goods
Durability: 08/31/2021
Lot identification:

088421204

Additional Information: Reference is made to the company’s customer information sheet attached.
Contact to the responsible authorities:

Baden-Württemberg: poststelle@mlr.bwl.de

Bavaria:poststelle@lgl.bayern.de
Hamburg:poststelle@justiz.hamburg.de
North Rhine-Westphalia:poststelle@mulnv.nrw.de
Schleswig-Holstein:poststelle@jumi.landsh.de
Press releases and information
title Attachment or web link
Customer information
affiche_consommateur E coli_de.pdf

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Posted in eae, STEC, STEC E.coli, STX 1, STX 2

Research – High Occurrence of Shiga Toxin-Producing Escherichia coli in Raw Meat-Based Diets for Companion Animals—A Public Health Issue

Posted on July 22, 2021 by | Leave a comment

MDPI

Feeding pets raw meat-based diets (RMBDs) is becoming increasingly popular but comes with a risk of pathogenic bacteria, including Shiga toxin-producing Escherichia coli (STEC). In humans, STEC may cause gastrointestinal illnesses, including diarrhea, hemorrhagic colitis (HC), and the hemolytic uremic syndrome (HUS). The aim of this study was to evaluate commercially available RMBDs with regard to the occurrence of STEC. Of 59 RMBD samples, 59% tested positive by real-time PCR for the presence of Shiga toxin genes stx1 and/or stx2. STECs were recovered from 41% of the 59 samples, and strains were subjected to serotyping and virulence gene profiling, using whole genome sequencing (WGS)-based methods. Of 28 strains, 29% carried stx2a or stx2d, which are linked to STEC with high pathogenic potential. Twenty different serotypes were identified, including STEC O26:H11, O91:H10, O91:H14, O145:H28, O146:H21, and O146:H28, which are within the most common non-O157 serogroups associated with human STEC-related illnesses worldwide. Considering the low infectious dose and potential severity of disease manifestations, the high occurrence of STEC in RMBDs poses an important health risk for persons handling raw pet food and persons with close contact to pets fed on RMBDs, and is of concern in the field of public health. View Full-Text

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Posted in E.coli O145, E.coli O26, eae, Food Micro Blog, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbiological Risk Assessment, Microbiology, O146, O91, raw pet food, STEC, STEC E.coli, STX 1, STX 2

Research – Shiga Toxin-Producing Escherichia coli (STEC) and STEC-Associated Virulence Genes in Raw Ground Pork in Canada

Posted on July 10, 2021 by | Leave a comment

Journal of Food Protection

Shiga toxin-producing Escherichia coli (STEC) O157:H7/NM and some non-O157 STEC are foodborne pathogens. In response to pork-associated O157 STEC outbreaks in Canada, we investigated the occurrence of STEC in Canadian retail raw ground pork during the period of November 1, 2014 and March 31, 2016. Isolated STEC were characterized to determine the Shiga-toxin gene ( stx ) subtype and the presence of virulence genes encoding intimin ( eae ), and enterohemorrhagic E. coli hemolysin (hlyA) . O157 STEC and non-O157 STEC were isolated from 0.11% (1/879) and 2.24% (13/580) of the pork samples. STEC virulence gene profiles containing both eae and hlyA were found only in the O157 STEC ( stx 2a , eae , hlyA ) isolate. The eae gene was absent from all non-O157 STEC isolates. Of the 13 non-O157 STEC isolates, two virulence genes of stx 1a and hlyA were found in four (30.8%) O91:H14 STEC isolates, while one virulence gene of stx 2e, stx 1a , and stx 2a was identified in five (38.5%), two (15.4%) and one (7.7%) STEC isolates respectively of various serotypes. The remaining non-O157 STEC isolate carried stx 2 , but the subtype is unknown as this isolate could not be recovered for sequencing. O91:H14 STEC ( stx 1a, hlyA ) was previously reported in association with diarrhea illnesses, while the other non-O157 STEC isolates identified in this study are not known to be associated with severe human illnesses. Virulence gene profiles identified in this study indicate that the occurrence of non-O157 STEC capable of causing severe human illness is rare in Canadian retail pork. However, O157 STEC in ground pork can occasionally occur, therefore education regarding the potential risks associated with STEC contamination of pork would be beneficial for the public and those in the food industry in order to help reduce foodborne illnesses.

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Posted in E.coli O157, E.coli O157:H7, eae, EHIC, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, microbial contamination, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, O91, Research, STX 1, STX 2

Research – Genetic diversity and pathogenic potential of Shiga toxin-producing Escherichia coli (STEC) derived from German flour

Posted on July 4, 2021 by | Leave a comment

Science Direct

Abstract

Shiga toxin-producing Escherichia coli (STEC) can cause severe human illness, which are frequently linked to the consumption of contaminated beef or dairy products. However, recent outbreaks associated with contaminated flour and undercooked dough in the United States and Canada, highlight the potential of plant based food as transmission routes for STEC. In Germany STEC has been isolated from flour, but no cases of illness have been linked to flour.

In this study, we characterized 123 STEC strains isolated from flour and flour products collected between 2015 and 2019 across Germany. In addition to determination of serotype and Shiga toxin subtype, whole genome sequencing (WGS) was used for isolates collected in 2018 to determine phylogenetic relationships, sequence type (ST), and virulence-associated genes (VAGs).

We found a high diversity of serotypes including those frequently associated with human illness and outbreaks, such as O157:H7 (stx2c/d, eae), O145:H28 (stx2a, eae), O146:H28 (stx2b), and O103:H2 (stx1a, eae). Serotypes O187:H28 (ST200, stx2g) and O154:H31 (ST1892, stx1d) were most prevalent, but are rarely linked to human cases. However, WGS analysis revealed that these strains, as well as, O156:H25 (ST300, stx1a) harbour high numbers of VAGs, including eae, nleB and est1a/sta1.

Although STEC-contaminated flour products have yet not been epidemiologically linked to human clinical cases in Germany, this study revealed that flour can serve as a vector for STEC strains with a high pathogenic potential. Further investigation is needed to determine the sources of STEC contamination in flour and flour products particularly in regards to these rare serotypes.

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Posted in E.coli O103, E.coli O145, E.coli O157, E.coli O157:H7, eae, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbiological Risk Assessment, Microbiology, Research, STEC, STEC E.coli, STX 1, STX 2

RASFF Alerts – STEC E.coli -Chilled Lamb Meat – Chilled Lamb Chops

Posted on April 23, 2021 by | Leave a comment

RASFF-Logo

RASFF

shigatoxin-producing Escherichia coli (stx2+, eae+, /25g) in chilled lamb meat from New Zealand in Italy

RASFF

shigatoxin-producing Escherichia coli (stx1 + stx2 /25g) in chilled lamb chops from Belgium in the Netherlands

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Posted in eae, food contamination, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Poisoning, food recall, Food Safety, Food Safety Alert, Food Testing, Food Toxin, RASFF, STEC, STEC E.coli, STX 1, STX 2

RASFF Alert – STEC E.coli – Chilled Beef Rump Tails

Posted on March 26, 2021 by | Leave a comment

European Food Alerts

RASFF

shigatoxin-producing Escherichia coli (O26 stx+ eae+ /25g) in chilled beef rump tails from Argentina in Germany

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Posted in Bacterial Toxin, E.coli O26, eae, food contamination, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Poisoning, food recall, Food Safety, Food Safety Alert, Food Testing, Food Toxin, microbial contamination, Microbiology, RASFF, STEC, STEC E.coli, STX 1, STX 2, Toxin, Uncategorized

Research – Escherichia coli in flour – sources, risks and prevention

Posted on February 26, 2021 by | Leave a comment

Click to access escherichia-coli-in-flour-sources-risks-and-prevention.pdf

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Posted in E.coli, E.coli O103, E.coli O121, E.coli O145, E.coli O157, E.coli O157:H7, E.coli O26, eae, EHEC, Food Microbiology Research, HUS, O103, Research, STEC, STEC E.coli, STX 1

RASFF Alert – STEC E.coli – Chilled Meat Preparation

Posted on January 29, 2021 by | Leave a comment

European Food Alerts

RASFF

Escherichia coli (eae+, stx1+, stx2-) in chilled meat preparation from Belgium in Belgium

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Posted in eae, food contamination, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Poisoning, food recall, Food Safety, Food Safety Alert, Food Testing, Food Toxin, RASFF, STEC, STEC E.coli, STX 1, STX 2

RASFF Alert – STEC E.coli – Lamb Carcasses

Posted on November 27, 2020 by | Leave a comment

European Food Alerts

RASFF

shigatoxin-producing Escherichia coli (stx+;eae+) in lamb carcasses from Belgium in Belgium

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Posted in Bacteria, bacterial contamination, eae, food contamination, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Poisoning, food recall, Food Safety, Food Safety Alert, Food Testing, Food Toxin, RASFF, STEC, STEC E.coli, STX 1, STX 2

Research – Microbiological Survey of Wheat Flour Sold at Retail in Canada, 2018-2019

Posted on November 15, 2020 by | Leave a comment

Journal of Food Protection

Following two O121 STEC outbreaks linked to wheat flour, this study was conducted to gain baseline information on the occurrence of bacterial pathogens and levels of indicator organisms in wheat flour in Canada. A total of 347 pre-packaged wheat flour samples were analyzed for Salmonella spp., Shiga Toxin-Producing Escherichia coli (STEC), Listeria monocytogenes ( L. monocytogenes ) , aerobic colony count (ACC), total coliforms, and generic Escherichia coli ( E. coli ) . Salmonella spp. and O157 STEC were not detected in any of the samples. L. monocytogenes was identified in two samples (0.6%) at levels below the limit of detection (<0.7 log CFU/g). Non-O157 STEC were isolated from six samples (1.7%) and were characterized for the presence of STEC virulence genes: stx 1, stx 2 and subtypes, eae , hlyA, and aggR . One O103:H25 STEC isolate carried virulence genes ( stx 1 a + eae ) that are known to be capable of causing diarrhea and/or bloody diarrhea in humans. Of the five remaining non-O157 STEC isolates, four carried single stx 2a or stx 2c genes and were considered to have the potential of causing diarrhea. The remaining non-O157 STEC isolate ( stx 2 ), while not a priority non-O157 STEC was not available for sequencing and thus its potential to cause illness is unknown. ACC, total coliforms, and generic E. coli were detected   in 98.8%, 72.6% and 0.6% of the flour samples. The mean counts of ACC were greater in whole-wheat flour as compared to the other flour types tested ( p <0.001). The results of this study suggest that the occurrence of O157 STEC and Salmonella  is low, but the occurrence of non-O157 STEC in wheat flour with the potential to cause human illness of diarrhea is relatively common. Therefore, the consumption of raw flour could increase the likelihood of STEC infections. Further research is merited for potential risk mitigation strategies within the food production system and with consumers.

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Posted in Coliforms, E.coli, E.coli O103, E.coli O121, E.coli O157, E.coli O157:H7, eae, food bourne outbreak, Food Microbiology Research, foodborne outbreak, foodbourne outbreak, outbreak, Research, Salmonella, STEC, STEC E.coli, STX 1, STX 2, Total Viable Count