Category Archives: STX 2

RASFF Alert – STEC E.coli – Minced Beef


RASFF – shigatoxin-producing Escherichia coli (stx+ eae+ /25g) in minced beef from Belgium in Belgium


RASFF Alert – STEC E.coli – Frozen Lamb Loins


RASFF – shigatoxin-producing Escherichia coli (O91 stx1+ stx2+ eae- /25g) in frozen lamb loins from New Zealand in Italy

RASFF Alert – STEC E.coli – Organic Raw Milk Goats Cheese – Soft Cheese – Raw Milk Goats Cheese


RASFF – shigatoxin-producing Escherichia coli (Stx1-, Stx2+, eae+, O103) in organic raw milk goat’s cheese from Belgium in Belgium

RASFF – shigatoxin-producing Escherichia coli (stx1+ stx2+ eae+ /25g) in soft cheese from Italy in the Netherlands

RASFF – shigatoxin-producing Escherichia coli (stx2a+ and stx2d+ /25g) in goat cheese with raw milk from France in Switzerland


Research – Complex Interactions Between Weather, and Microbial and Physicochemical Water Quality Impact the Likelihood of Detecting Foodborne Pathogens in Agricultural Water


Agricultural water is an important source of foodborne pathogens on produce farms. Managing water-associated risks does not lend itself to one-size-fits-all approaches due to the heterogeneous nature of freshwater environments. To improve our ability to develop location-specific risk management practices, a study was conducted in two produce-growing regions to (i) characterize the relationship between Escherichia coli levels and pathogen presence in agricultural water, and (ii) identify environmental factors associated with pathogen detection. Three AZ and six NY waterways were sampled longitudinally using 10-L grab samples (GS) and 24-h Moore swabs (MS). Regression showed that the likelihood of Salmonella detection (Odds Ratio [OR] = 2.18), and eaeA-stx codetection (OR = 6.49) was significantly greater for MS compared to GS, while the likelihood of detecting L. monocytogenes was not. Regression also showed that eaeA-stx codetection in AZ (OR = 50.2) and NY (OR = 18.4), and Salmonella detection in AZ (OR = 4.4) were significantly associated with E. coli levels, while Salmonella detection in NY was not. Random forest analysis indicated that interactions between environmental factors (e.g., rainfall, temperature, turbidity) (i) were associated with likelihood of pathogen detection and (ii) mediated the relationship between E. coli levels and likelihood of pathogen detection. Our findings suggest that (i) environmental heterogeneity, including interactions between factors, affects microbial water quality, and (ii) E. coli levels alone may not be a suitable indicator of food safety risks. Instead, targeted methods that utilize environmental and microbial data (e.g., models that use turbidity and E. coli levels to predict when there is a high or low risk of surface water being contaminated by pathogens) are needed to assess and mitigate the food safety risks associated with preharvest water use. By identifying environmental factors associated with an increased likelihood of detecting pathogens in agricultural water, this study provides information that (i) can be used to assess when pathogen contamination of agricultural water is likely to occur, and (ii) facilitate development of targeted interventions for individual water sources, providing an alternative to existing one-size-fits-all approaches.

RASFF Alert – STEC E.coli – Organic Leak Seeds for Sprouting


RASFF – enteropathogenic Escherichia coli (stx- eae+ /25g) in organic leek seeds for sprouting from China, via Italy in Norway

RASFF Alert – STEC E.coli – Chilled Bovine Carcass


RASFF – shigatoxin-producing Escherichia coli (stx1+, stx2+, eae+ /25g) in chilled bovine carcass from Belgium in Belgium

RASFF Alert- STEC E.coli – Chilled Beef Steak Tartare


RASFF – shigatoxin-producing Escherichia coli (stx2+ /25g) in chilled beef steak tartare from Poland in Poland

RASFF Alert – STEC E.coli – Raw Milk Cheese


RASFF – shigatoxin-producing Escherichia coli (stx2+ eae /25g) in raw milk cheese from France, packaged in Germany in Germany

Research – Shiga toxin-producing Escherichia coli (STEC) and food: attribution, characterization, and monitoring


Strains of pathogenic Escherichia coli that are characterized by their ability to
produce Shiga toxins are referred to as Shiga toxin-producing E. coli (STEC). STEC
are an important cause of foodborne disease and infections have been associated with a wide range of human clinical illnesses ranging from mild non-bloody
diarrhoea to bloody diarrhoea (BD) and haemolytic uraemic syndrome (HUS)
which often includes kidney failure. A high proportion of patients are hospitalized,
some develop end-stage renal disease (ESRD) and some die.
The Codex Committee on Food Hygiene (CCFH) has discussed the issue of STEC
in foods since its 45th Session, and at the 47th Session, in November 2015, it was
agreed that it was an important issue to be addressed (REP 16/FH, 2015)2
. To
commence this work, the CCFH requested the Food and Agriculture Organization
(FAO) and the World Health Organization (WHO) to develop a report compiling
and synthesizing available relevant information, using existing reviews where
possible, on STEC. The CCFH noted that further work on STEC in food, including
the commodities to be focused on, would be determined based on the outputs of
the FAO/WHO consultation.
The information requested by CCFH is divided into three main areas: the global
burden of disease and source attribution; hazard identification and characterization; and monitoring, including the status of the currently available analytical
methods. This report provides an overview of the work undertaken in response to
the request from the CCFH and provides the conclusions and advice of the Expert
Group based on the currently available information.

RASFF Alert – STEC E.coli – Chilled Spelt Dough


RASFF – shigatoxin-producing Escherichia coli (stx1-, stx2+ /25g) in chilled spelt dough from Austria in Germany