Category Archives: STX 2

RASFF Alert – STEC E.coli – Chilled Spelt Dough

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RASFF – shigatoxin-producing Escherichia coli (stx1-, stx2+ /25g) in chilled spelt dough from Austria in Germany

Research -Occurrence of Campylobacter spp., Salmonella spp. and shiga toxin‐producing Escherichia coli in inline milk filters from Swedish dairy farms

Wiley Online

Abstract

This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from 12.6% of filters. The stx genes (stx 1stx 2, or both) were screened from 71% of the filters and STEC was isolated from 14% of these. Of the STEC isolates, 21 contained the stx 1 gene, 19 the stx 2 gene, and five a combination of both stx 1 and stx 2 genes. Whole genome sequence typing on 34 of the 45 STEC showed that they belonged to 21 different serotypes, of which STEC O145:H28 was the most common (2%). STEC O157:H7 was only found from one (0.3%) of the filters. A combination of stx 2 and eae genes was found from 0.7% of the total number of inline milk filters analyzed, while stx 2a was found in 24% of the whole genome‐sequenced isolates. There was a significant positive correlations between number of animals per farm and presence of pathogens on milk filters.

Research – Survey of Intact and Nonintact Raw Pork Collected at Retail Stores in the Mid-Atlantic Region of the United States for the Seven Regulated Serogroups of Shiga Toxin–Producing Escherichia coli

Journal of Food Protection

ABSTRACT

A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga toxin–producing Escherichia coli (STEC-7) using standard microbiological and molecular methods. In short, 21 (5.3%) of the 395 ground or nonintact pork samples and 3 (3.4%) of the 119 intact pork samples tested positive via the BAX system real-time PCR assay for the stx and eae virulence genes and for the somatic O antigens for at least one of the STEC-7 serogroups. However, none of these 24 presumptive-positive pork samples subsequently yielded a viable isolate of STEC displaying a STEC-7 serogroup-specific surface antigen in combination with the stx and eae genes. These data suggest that cells of STEC serogroups O26, O45, O103, O111, O121, O145, or O157:H7 are not common in retail raw pork samples in the mid-Atlantic region of the United States.

HIGHLIGHTS
  • None of the 514 retail raw pork samples were positive for STEC-7.

  • Four of 514 raw pork samples harbored E. coli of unknown serogroup containing stx and eae.

  • STEC-7 are uncommon in retail raw pork samples in the U.S. mid-Atlantic region.

 

RASFF Alert – STEC E.coli – Frozen VP Buffalo Sausages

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RASFF – shigatoxin-producing Escherichia coli (stx1+ stx2+ /25g) in frozen vacuum-packed buffalo sausages from Belgium in the Netherlands

RASFF Alerts – STEC E.coli – Lamb Carcase – Bovine Meat

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RASFF -enteropathogenic Escherichia coli (O157 stx1+, stx2-, eae+) in lamb carcass from Belgium in Belgium

RASFF -shigatoxin-producing Escherichia coli (O103 stx1+ stx2- eae+ /25g) in frozen bovine meat from Uruguay in Finland

RASFF Alerts – STEC E.coli – Frozen Beef

Last two weeks catch up

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RASFF – shigatoxin-producing Escherichia coli (stx1+ /25g) in frozen boneless beef topside from Brazil in the Netherlands

RASFF – shigatoxin-producing Escherichia coli ( stx2+ eae-; stx2+ eae+ present /25g) in frozen beef from Uruguay in Finland

RASFF Alert -STEC E.coli – Chilled Beef

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RASFF – shigatoxin-producing Escherichia coli (stx1+, stx2+,) in chilled beef from Argentina in Italy