Category Archives: eae

RASFF Alerts – STEC E.coli – Raw Milk Cheese – Camembert Cheese

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RASFF – shigatoxin-producing Escherichia coli (stx2+ /25g) in raw milk cheese from France in Germany

RASFF – enteropathogenic Escherichia coli (O26:H11 stx- eae+ /25g) in camembert cheese from France in France

RASFF Alerts – STEC E.coli – E.coli O157 – Raw Milk Cheese

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RASFF – shigatoxin-producing Escherichia coli (O157:H7 stx+ eae+) in raw milk cheese from France in France

RASFF – shigatoxin-producing Escherichia coli in raw milk cheese from France in Germany

RASFF Alert- STEC E.coli – 026 – 0103 – Frozen Minced Beef

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RASFF – enteropathogenic Escherichia coli (O26:H11 O103:H2 eae+ stx- /25g) in frozen minced beef from the United Kingdom in France

Research – Detection, Isolation, and Characterization of Shiga Toxin–Producing Escherichia coli in Flour

Journal of Food Protection

Wheat flour has recently been described as a novel vehicle for transmission of Shiga toxin–producing Escherichia coli (STEC). Very recently, an outbreak of STEC O121 and STEC O26 infections was linked to flour in the United States. The aim of the present study was to generate baseline data for the occurrence of STEC in flour samples from different retailers in Switzerland. In total, 70 flour samples were analyzed. After enrichment, the samples were screened for stx1 and stx2 by the Assurance GDS MPX ID assay. STEC strains were isolated and serotyped by the E. coli SeroGenoTyping AS-1 kit. The determination of stx subtypes was performed with conventional PCR amplification. Screening for eae, aggR, elt, and estIa/Ib was performed by real-time PCR. Nine (12.9%) of the flour samples tested positive for stx by PCR. STEC was recovered from eight (88.9%) of the positive samples. Two isolates were STEC O11:H48 harboring stx1c/stx1d, two were O146:H28 containing stx2b, one was O103:H2 containing stx1a and eae, and three were O nontypeable: Ont:H12 (stx2a), Ont:H14 (stx2a/stx2g), and Ont:H31 (stx1c/stx1d). STEC O103 belongs to the “top five” serogroups of human pathogenic STEC in the European Union, and STEC O146 is frequently isolated from diseased humans in Switzerland. Our results show that flour may be contaminated with a variety of STEC serogroups. Consumption of raw or undercooked flour may constitute a risk for STEC infection.

RASFF Alert – STEC E.coli – O26 – Raw Goats Milk Cheese

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RASFF -enteropathogenic Escherichia coli (026H11 eae+, stx-) in raw goat’s milk cheese from France in France

RASFF Alert- STEC E.coli – E.coli – Staphylococcus aureus – Raw Milk Soft Cheese

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RASFF -shigatoxin-producing Escherichia coli (stx1+; stx2+; aea+) and Staphylococcus aureus (47000 CFU/g) and high count of Escherichia coli (60000 CFU/g) in soft cheese made from raw milk from France

RASFF Alerts – Backdated 22/9/18 – 05/10/18 – STEC E.coli – Roastbeef – Chilled VP Beef – Frozen Boneless Beef

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RASFF-shigatoxin-producing Escherichia coli (stx1+; stx2+; eae-; aatA- /25g) in roastbeef from Argentina in Germany

RASFF-shigatoxin-producing Escherichia coli (stx2+ /25g) in chilled vacuum-packed beef from Argentina in Italy

RASFF-shigatoxin-producing Escherichia coli (stx2+ /25g) in frozen boneless beef from Brazil in Italy