Category Archives: eae

Research – Survey of Intact and Nonintact Raw Pork Collected at Retail Stores in the Mid-Atlantic Region of the United States for the Seven Regulated Serogroups of Shiga Toxin–Producing Escherichia coli

Journal of Food Protection

ABSTRACT

A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga toxin–producing Escherichia coli (STEC-7) using standard microbiological and molecular methods. In short, 21 (5.3%) of the 395 ground or nonintact pork samples and 3 (3.4%) of the 119 intact pork samples tested positive via the BAX system real-time PCR assay for the stx and eae virulence genes and for the somatic O antigens for at least one of the STEC-7 serogroups. However, none of these 24 presumptive-positive pork samples subsequently yielded a viable isolate of STEC displaying a STEC-7 serogroup-specific surface antigen in combination with the stx and eae genes. These data suggest that cells of STEC serogroups O26, O45, O103, O111, O121, O145, or O157:H7 are not common in retail raw pork samples in the mid-Atlantic region of the United States.

HIGHLIGHTS
  • None of the 514 retail raw pork samples were positive for STEC-7.

  • Four of 514 raw pork samples harbored E. coli of unknown serogroup containing stx and eae.

  • STEC-7 are uncommon in retail raw pork samples in the U.S. mid-Atlantic region.

 

RASFF Alerts – STEC E.coli – Lamb Carcase – Bovine Meat

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RASFF -enteropathogenic Escherichia coli (O157 stx1+, stx2-, eae+) in lamb carcass from Belgium in Belgium

RASFF -shigatoxin-producing Escherichia coli (O103 stx1+ stx2- eae+ /25g) in frozen bovine meat from Uruguay in Finland

RASFF Alerts – STEC E.coli – Frozen Beef

Last two weeks catch up

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RASFF – shigatoxin-producing Escherichia coli (stx1+ /25g) in frozen boneless beef topside from Brazil in the Netherlands

RASFF – shigatoxin-producing Escherichia coli ( stx2+ eae-; stx2+ eae+ present /25g) in frozen beef from Uruguay in Finland

RASFF Alerts – STEC E.coli – Soft Cheese

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RASFF – shigatoxin-producing Escherichia coli (stx1+ stx2- eae-) in soft cheese from Belgium in Germany

RASFF Alert – STEC E.coli – Chilled Beef

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RASFF – shigatoxin-producing Escherichia coli (stx+ eae+ /25g) in chilled beef from Belgium in Belgium

RASFF Alert – STEC E.coli 026- Frozen Meat for Minced Meat –

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RASFF – shigatoxin-producing Escherichia coli (stx1+ stx2+ eae+ O26:H11 /25g) in frozen meat for minced meat from Ireland in France

RASFF Alert – STEC E.coli – Frozen Beef

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RASFF – shigatoxin-producing Escherichia coli (stx1+; stx2-; eae+ /25g) in frozen beef from Brazil in Italy