Steak tartare is a raw, ready-to-eat meal popular in European countries, the safety of which is often discussed due to the risk of foodborne illness. The aim of this study was to determine the prevalence of Listeria monocytogenes in vacuum-packed steak tartare from retailers in the Czech Republic, characterize the strains obtained by typing methods and to evaluate the efficacy of ListexTM P100 against L. monocytogenes artificially inoculated into steak tartare samples. The prevalence of L. monocytogenes was 55% and 17 isolates belonging mostly to serotype 1/2a were obtained. Altogether 11 sequence types and 11 clonal complexes were assigned based on the whole genome sequencing (WGS) signifying the high diversity of L. monocytogenes isolates obtained. Core genome multi-locus sequence typing (cgMLST) did not confirm an epidemiological connection with human cases of listeriosis. The efficacy of ListexTM P100 treatment at concentrations of 108 and 109 PFU/g on artificially inoculated beef steak tartare samples was not efficient. Based on the results of this study, steak tartare from retailers can be considered as a source of L. monocytogenes that remains a challenge to the food industry. View Full-Text
NELLORE: At least 15 children fell sick after they reportedly ate adulterated food at an Anganwadi in Podalakuru town of Nellore district. According to reports, the children were suffering from diarrhoea since Wednesday evening. Parents staged a demonstration at the Anganwadi and blamed the negligent attitude of the staff for delaying treatment to their children.
They alleged that the children began vomiting and fell sick after eating food provided at the Anganwadi. They said the children were admitted to a local hospital for treatment where doctors confirmed that they fell sick due to the adulterated food they ate. The children are getting treated at various hospitals in Podalakuru and Nellore city. Sources said their condition is stable now.




