Category Archives: Food Microbiology Research

UK – Listeria legislation not broken – don’t fix it!

Chilled Food Association

CFA, its members and their retail customers, have long been at the forefront of the development
of best practice, control and regulation of Listeria monocytogenes (Lm) in the UK, Europe and beyond. CFA’s various Listeria guidance documents are available as free downloads.

European listeriosis data consistently show UK rates to be half of that for Europe overall. Outside the UK, commercial enforcement by customers is often lacking and differing interpretations of regulations lead to a lack of consistent compliance.

Against this background the European Commission is expected to propose changes to Lm legislation (EU Microbiological Criteria for Foodstuffs Regulation 2073/2005). This is expected to require challenge testing to set shelf life, rather than the established and proven effective Day of Production (DOP) and End of Life (EOL) approach, coupled with storage trials.

The expected changes will particularly affect chilled foods made on the Continent, where shelf lives are substantially longer than those in the UK’s tightly controlled local market – but changes would also impact export to the EU. Increased waste would result from consequent reduced shelf lives, with increased prices from highly specialised and narrowly applicable testing – all with questionable food safety benefits.

“The EU’s approach is also flawed in that it only covers the testing of food and does not address critical hygienic control of the food production environment. We can see no obvious public health or sustainability benefit to the changes and will continue to lobby the EC to retain the DOP/EOL and storage trial approach as it is demonstrably highly effective. In short, when it comes to European Listeria legislation – it’s not broken, please don’t fix it!”

USA – The amount of coastal water that can harbor harmful Vibrio bacteria has spiked 56%. One species is flesh-eating.

Business Insider

KSWFOODWORLD

The amount of coastal water in which harmful bacteria can live has increased 56% over the past few decades, a report published Wednesday found.

That bacteria family, called Vibrio, lives in salty or brackish coastal waters, including in the US and Canada. The infection it causes, vibriosis, is usually contracted by eating raw or undercooked seafood or by exposing a wound to bacteria-infested seawater. Mild cases resolve in about three days, but Vibrio can also cause severe diseases, including gastroenteritis, life-threatening cholera, dangerous wound infections, and sepsis.

One species of Vibrio bacteria, Vibrio vulnificus, is referred to as flesh-eating because the bacteria can aggressively destroy body tissue. Those infections, though rare, often require intensive care or amputation. And they can be fatal, killing one in five infected people, usually within two days, according to the Centers for Disease Control and Prevention.

The reasons Vibrio is becoming a greater threat are that sea surface temperatures are rising and seawater is getting saltier. That’s one of many alarming findings from the medical journal The Lancet’s sixth annual report on health and climate change. In it, researchers from academia and the United Nations tracked 44 indicators of health effects linked to climate change.

Belgium – Roast shoulder of marinated deer 500G – Salmonella

AFSCA

Recall of Aldi
Product: Roast shoulder of marinated deer 500G.
Problem: Possible presence of Salmonella.


PRODUCT DESCRIPTION

Name: Roast shoulder of marinated deer 500G
EAN code: 2007030022628
Type of packaging: vacuum-packed
Expiration dates (DLC): 24/10/2021 & 26/10/2021
Lot code: 21281
Sales period: from 10/11/2021 to 10/19/2021
Points of sale: ALDI stores

NATURE OF THE PROBLEM

Possible presence of Salmonella in the above article.

ALDI has therefore decided, in consultation with the AFSCA, to withdraw this product from the market and to recall it to consumers.

Other ALDI products are not affected by this product recall.

DESCRIPTION OF THE RISK

Possible symptoms of salmonella poisoning are fever, abdominal cramps and diarrhea, within 12 to 48 hours of consuming the contaminated food. The risk of infection is higher in the elderly, children, pregnant women and people with weakened immune systems.

People who have consumed these products and who present this type of symptoms are invited to consult their doctor without delay, notifying him of this consumption.

TIPS FOR CONSUMERS

Please do not consume these products and bring them back to the store.
The returned products will be reimbursed (also without sales receipt).
For any further information, you can contact ALDI via serviceclients@aldi.be .

Research – Prevalence of E. coli Sequence Type 131as a foodborne pathogen in Swiss chicken

Click to access Wetzel%20and%20Fieseler_E.%20coli%20ST131_Prevalence.pdf

Research – Effect of Direct Viral–Bacterial Interactions on the Removal of Norovirus From Lettuce

Frontiers in Microbiology

Norovirus Food Safety kswfoodworld

Norovirus (NoV) is the main non-bacterial pathogen causing outbreaks of gastroenteritis and is considered to be the leading cause of foodborne illness. This study aims to determine whether lettuce-encapsulated bacteria can express histo-blood group antigen (HBGA)–like substances to bind to NoV and, if so, to explore its role in protecting NoV from disinfection practices. Fifteen bacterial strains (HBGA-SEBs) were isolated from the lettuce microbiome and studied as they were proved to have the ability to express HBGA-like substances through indirect ELISA detection. By using attachment assay, HBGA-SEBs showed great abilities in carrying NoVs regarding the evaluation of binding capacity, especially for the top four strains from genera WautersiellaSphingobacterium, and Brachybacterium, which could absorb more than 60% of free-flowing NoVs. Meanwhile, the direct viral–bacterial binding between HBGA-like substance-expressing bacteria (HBGA-SEB) and NoVs was observed by TEM. Subsequently, results of simulated environmental experiments showed that the binding of NoVs with HBGA-SEBs did have detrimental effects on NoV reduction, which were evident in short-time high-temperature treatment (90°C) and UV exposure. Finally, by considering the relative abundance of homologous microorganisms of HBGA-SEBs in the lettuce microbiome (ca. 36.49%) and the reduction of NoVs in the simulated environments, we suggested putting extra attention on the daily disinfection of foodborne-pathogen carriers to overcome the detrimental effects of direct viral–bacterial interactions on the reduction of NoVs.

Research – Risk evaluation of E. coli ST 131 as a foodborne pathogen in Switzerland

BLV

Within recent years, the topic of multidrug-resistant, uropathogenic Escherichia coli strains has seen a rise in occurrence as foodborne pathogens. At the core of this topic is the specific clonal group referred to as Escherichia coli O25b:H4 sequence type 131 (ST 131). It is an extended-spectrum β-lactamase (ESBL) producing E. coli strain, postulated to be responsible for the spread of ESBL-encoding genes world-wide.
This literature review aimed to evaluate ST 131 as a foodborne pathogen in Switzerland, in order to assess the risk it poses for food producers in Switzerland specifically. On a global scale, ST 131 has been identified on all continents. It is mostly associated with chicken and poultry meat, and has been isolated from retail products many times.
Not exclusive to chicken, it was rarely identified from fish guts and gills. In all other meat products, ST 131 was found only in faecal matter, not in the product itself. Besides meat, the pathogen was not identified from any sources such as dairy, fruit and vegetables.
The situation is similar in Switzerland, where it was isolated frequently from chicken, rarely from fish and a complete absence of the pathogen in all other food product groups. The risk, ST 131 poses towards Swiss food producers has been evaluated as a medium risk factor for both chicken and fish products, and a low risk factor for any other products. Despite ST 131 being evaluated as a medium-to-low risk factor, depending on the product, it is still recommended to perform further research on the topic. Especially looking towards prevalence in Swiss food, in chicken and poultry meat, but also in fish.
Specifically fish designated for raw consumption (Sushi, Sashimi). Moreover, the spread of afore-mentioned ESBL-encoding genes is hypothesized to occur also during infection of humans. This leads to the recommendation, that ST 131 should be considered a food-safety risk in all products, in order to eliminate said spread. Whether this consideration as a food-safety risk is feasible, cannot be said without further analysis of products and viable treatment options.

India – FSSAI guidelines for sampling for microbiological analysis

FNB News

The Food Safety and Standards Authority of India (FSSAI) has issued a draft on guidelines for sampling for microbiological analysis.

The guidelines prescribe the general requirement for microbiological sampling, sampling plan, sampling tools, equipment & material, sampling procedure, labelling of sample, storage & transportation and food category wise sampling procedures.

According to the FSSAI, the microbiological analysis was vital to verify the compliance with the criteria laid down in Food Safety and Standards Regulations, food safety management system and process control, compliance of individual batches, and general information on the microbiological status of certain products placed on the market. Further, the analysis will help in monitoring and surveillance of food products along with investigation of suspected food-borne outbreaks, customer complaints.

Ashwin Bhadri, CEO, Equinox Labs, says that the sampling process ensures that the food quality hasn’t been compromised. Sampling is one of the best ways to know in which state food can be preserved for consumption without contamination.

He said, “For the betterment of people, adhering to the regulatory guidelines when it comes to sampling becomes essential. Consumer safety is foremost and following those policies assures that they are safeguarded. In India, FSSAI has introduced guidelines that every food product should undergo microbiology sampling before it is consumed.”

He explains, “It examines the compliance of individual batches. Microbiological sampling is undertaken by authorised people who are experienced and trained in those processes. They select the samples from the huge lot that allows a sample to stand for a whole lot and give the right decision concerning quality, contamination and shelf life of food products. Sampling and testing are absolutely necessary as it helps to verify the compliance laid down as per the Food Safety and Standards Regulations. The procedure of microbiological sampling and testing allows the investigation of customer complaints and also to suspect food-borne outbreaks if any.”

The guidelines were approved by the Scientific Panel on Methods of Sampling & Analysis in a meeting recently.  And FSSAI has sought comments from the stakeholders on the same.

USA – USDA Launches New Effort to Reduce Salmonella Illnesses Linked to Poultry

USDA

he U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) today announced that it is mobilizing a stronger, and more comprehensive effort to reduce Salmonella illnesses associated with poultry products. The agency is initiating several key activities to gather the data and information necessary to support future action and move closer to the national target of a 25% reduction in Salmonella illnesses.

“Far too many consumers become ill every year from poultry contaminated by Salmonella,” said Agriculture Secretary Tom Vilsack. “We need to be constantly evolving in our efforts to prevent foodborne illness to stay one step ahead of the bad bugs. Today we’re taking action to help prevent Salmonella contamination throughout the poultry supply chain and production system to protect public health.”

Despite consistent reductions in the occurrence of Salmonella in poultry products, more than 1 million consumer illnesses due to Salmonella occur annually, and it is estimated (PDF, 1.4 MB) that over 23% of those illnesses are due to consumption of chicken and turkey.

“Reducing Salmonella infections attributable to poultry is one of the Department’s top priorities,” said USDA Deputy Under Secretary Sandra Eskin, who is leading the initiative. “Time has shown that our current policies are not moving us closer to our public health goal. It’s time to rethink our approach.”

USDA intends to seek stakeholder feedback on specific Salmonella control and measurement strategies, including pilot projects, in poultry slaughter and processing establishments. A key component of this approach is encouraging preharvest controls to reduce Salmonella contamination coming into the slaughterhouse. The data generated from these pilots will be used to determine if a different approach could result in a reduction of Salmonella illness in consumers.

The effort will leverage USDA’s strong research capabilities and strengthen FSIS’ partnership with the Research, Education and Economics (REE) mission area to address data gaps and develop new laboratory methods to guide future Salmonella policy. Meanwhile, the National Advisory Committee for Microbiological Criteria in Foods, an independent federal advisory committee, will be asked to advise on how FSIS can build on the latest science to improve its approach to Salmonella control. Since it is not just the presence or absence of Salmonella, but the quantity of bacteria that can impact the likelihood of illness, FSIS will examine how quantification can be incorporated into this approach. Moreover, with emerging science suggesting that not all Salmonella are equally likely to cause human illness, FSIS will focus on the Salmonella serotypes and the virulence factors that pose the greatest public health risk.

Moving forward, this initiative will require collaboration and ongoing dialogue with stakeholders — industry, consumer groups, and researchers alike. USDA looks forward to working closely with stakeholders on informing and implementing key activities of this framework in the very near future.

USDA touches the lives of all Americans each day in so many positive ways. In the Biden-Harris Administration, USDA is transforming America’s food system with a greater focus on more resilient local and regional food production, fairer markets for all producers, ensuring access to safe, healthy and nutritious food in all communities, building new markets and streams of income for farmers and producers using climate smart food and forestry practices, making historic investments in infrastructure and clean energy capabilities in rural America, and committing to equity across the Department by removing systemic barriers and building a workforce more representative of America. To learn more, visit www.usda.gov.

Research – Multi-country outbreak of Salmonella Typhimurium and S. Anatum infections linked to Brazil Nuts

ECDC

Click to access salmonella-typhimurium-rapid-outbreak-assessment-october-2020.pdf

Research – Nisin-based antimircobial combination with cold plasma treatment inactivate Listeria monocytogenes on Granny Smith apples

Science Direct

Abstract

A nisin-based antimicrobial and cold plasma combination treatments in reducing Listeria monocytogenes inoculated on apple surfaces purchased from a New Jersey farm and a supermarket in Philadelphia area was investigated. All apples were spot inoculated or by submersion in 107 CFU/ml L. monocytogenes inoculum. Populations of L. monocytogenes recovered on farm and supermarket apples after spot inoculation averaged 5.8 ± 0.24 log CFU/g and 4.6 ± 0.12 log CFU/g, respectively and 4.1 ± 0.22 log CFU/g and 3.6 ± 0.12 log CFU/g, respectively on submerged apples. All apples were treated with antimicrobial solution for 30 s, 40 s, 3 min (180s) and 1 h (3600 s), cold plasma treatments for 30 and 40s, and a combination of antimicrobial and cold plasma treatments and the surface structure of apples were examined using scanning electron microscopy (SEM). Cold plasma treatment at 40s, followed immediately with antimicrobial treatments at 180s and 3600 s led to 2.5 and 4.6 log CFU/g inactivation of L. monocytogenes, respectively. SEM observation showed changes on apple surface structures but not on bacterial cell structure. This result suggests that this combination treatments is effective in killing L. monocytogenes on apple surfaces.