Research – Scientific Evidence Supports the Use of Alcohol Based Hand Sanitizers as an Effective Alternative to Handwashing in Retail Food and Foodservice Settings When Heavy Soiling is Not Present on Hands

Journal of Food Protection

Suboptimal food worker health and hygiene has been a common contributing factor in foodborne disease outbreaks for many years. Despite clear FDA Model Food Code recommendations for hand washing and glove use, food worker compliance with handwashing recommendations has remained poor for more than 20 years. Food workers’ compliance with recommended handwashing is adversely impacted by a number of barriers, including complaints of time pressure, inadequate number and/or location of handwashing sinks and handwashing supplies, lack of food knowledge and training regarding handwashing, the belief that wearing gloves obviates the need for hand washing, insufficient management commitment, and adverse skin effects caused by frequent handwashing. While many of the issues related to poor handwashing practices in food service facilities are the same as those in healthcare settings, a new approach to healthcare hand hygiene was deemed necessary over 15 years ago due to persistently low compliance rates among healthcare personnel. Evidence-based hand hygiene guidelines for healthcare settings were published by both CDC in 2002 and by the World Health Organization in 2009. Despite similar low handwashing compliance rates among retail food establishment workers, no changes in the Food Code indications for handwashing have been made since 2001. In direct contrast to healthcare settings, where frequent use of alcohol-based hand sanitizers (ABHSs) in lieu of handwashing has improved hand hygiene compliance rates and reduced infections, the Food Code continues to permit the use of ABHSs only after hands have been washed with soap and water. This general interest manuscript provides clear evidence to support modifying the FDA Model Food Code to  allow the use of ABHS as an acceptable alternative to handwashing in situations where heavy soiling is not present . Emphasis on the importance of handwashing when hands are heavily soiled and appropriate use of gloves are still indicated.

Research – Virulence Characterization of Listeria monocytogenes, Listeria innocua, and Listeria welshimeri Isolated from Fish and Shrimp Using In Vivo Early Zebrafish Larvae Models and Molecular Study

MDPI

Listeriosis is one of the most notable foodborne diseases and is characterized by high rates of mortality. L. monocytogenes is the main cause of human listeriosis outbreaks, however, there are isolated cases of disease caused by other species of the genus Listeria. The aim of this study was to evaluate strains of L. monocytogenes (n = 7), L. innocua (n = 6), and L. welshimeri (n = 2) isolated from fish and shrimps for their virulence based on the presence of virulence genes and the in vivo Danio rerio (zebrafish) larvae models. A total of 15 strains were analyzed. The zebrafish larvae model showed that the larvae injected with L. monocytogenes strains were characterized by the lowest survival rate (46.5%), followed by L. innocua strains (64.2%) and L. welshimeri (83.0%) strains. Multiplex PCRs were used for detection of selected virulence genes (luxS, actA2, prfA, inlB, rrn, iap, sigB, plcB, actA, hlyA), the majority of which were present in L. monocytogenes. Only a few virulence-related genes were found in L. welshimeri, however, no correlation between the occurrence of these genes and larval survival was confirmed. This research highlights the importance of the potential impact that Listeria spp. strains isolated from fish and shrimps may have on consumers. View Full-Text

Research – Effect of Time, Temperature and Transport Media on the Recovery of Listeria monocytogenes from Environmental Swabs

Journal of Food Protection

Environmental monitoring for Listeria monocytogenes in food processing environments is key for ensuring the safety of ready-to-eat foods. For sampling, swabs are often hydrated with a wetting or transport medium which may contain neutralizers and other ingredients. After swabbing the environment, the swabs may then be transported or shipped cold to an off-site laboratory for testing, ideally within 48 h. Extended shipping times may subject the pathogen to increased temperatures in the presence of the wetting medium, organics, and other chemicals from the processing facility which may confound detection. This study evaluated growth and detection of L. monocytogenes on stainless steel exposed to either buffer or sodium hypochlorite prior to drying. Swabs were rehydrated with Butterfield’s Phosphate Buffer, Neutralizing Buffer, Letheen Broth or Dey-Engley Neutralizing Broth prior to swabbing. Swabs were stored in the presence of no added food, cheese whey or ice cream under both optimal (4°) and sub-optimal (15°C) temperatures for up to 72 h. Overall, there was no growth of L. monocytogenes at 4°C through 72 h storage, though enrichment from these swabs was dependent on the presence and type of food matrix. Pathogen growth during storage at 15°C was more variable and depended on both the food matrix and transport media used, with Dey-Engley and Letheen Broth allowing for the highest population increases. Overall, more enrichments resulting in L. monocytogenes detections were observed when using Letheen Broth and Neutralizing Buffer than Dey-Engley which resulted in fewer detections at 15°C. Logistic regression and Cochran-Mantel-Haenszel (CMH) analyses determined that storage temperature, transport media, and food matrix all significantly affected detection of L. monocytogenes , while storage time did not have a clear effect on recovery from swabs.

Research – Impacts of Wet Market Modernization Levels and Hygiene Practices on the Microbiome and Microbial Safety of Wooden Cutting Boards in Hong Kong

MDPI

Accessing food through wet markets is a common global daily occurrence, where fresh meat can be purchased to support an urbanizing world population. Similar to the wet markets in many other metropolitan cities in Asia, Hong Kong wet markets vary and are characterized by differing hygiene routines and access to essential modern technologies. The lack of risk assessments of food contact surfaces in these markets has led to substantial gaps in food safety knowledge and information that could help improve and maintain public health. Microbial profiling analyses were conducted on cutting boards that had been used to process pork, poultry, and seafood at 11 different wet markets. The markets differed in hygiene protocols and access to modern facilities. Irrespective of whether wet markets have access of modern infrastructure, the hygiene practices were largely found to be inefficient based on the prevalence of bacterial species typically associated with foodborne pathogens such as Campylobacter fetus, Clostridium perfringens, Staphylococcus aureus, and Vibrio parahaemolyticus; indicator organisms such as Escherichia coli; as well as nonfoodborne pathogenic bacterial species potentially associated with nosocomial infections, such as Klebsiella pneumoniae and Enterobacter cloacae. Other Vibrio species, V. parahaemolyticus and V. vulnificus, typically associated with contaminated raw or undercooked seafood with the potential to cause illness in humans, were also found on wooden cutting boards. This study indicated that the hygienic practices used in Hong Kong wet markets are not sufficient for preventing the establishment of spoilage or pathogenic organisms. This study serves as a basis to review current hygiene practices in wet markets and provides a framework to reassess existing safety protocols.

RASFF Alerts – Salmonella – Chicken Doner – Chicken Elements – Chicken Breast Fillets – Chorizo – Goose Breast – Turkey Meat – Minced Beef and Beef Cuts – Organic Flaxseed – Betel Leaves

European Food Alerts

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Salmonella (present) in chicken doner from the Netherlands in the Netherlands

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Salmonella enterica ser. Enteritidis (in 1 out of 5 samples /25g) in chilled chicken elements from Poland in Poland

RASFF

Salmonella enterica ser. Infantis (presence /25g) in chicken breast fillets from Poland in Hungary

RASFF

Salmonella enterica ser. Rissen (presence /25g) in chorizo from Spain, via Germany in Switzerland

RASFF

Salmonella (presence /25g) in frozen goose breast from Hungary in Sweden

RASFF

Salmonella enterica ser. Bredeney (in 1 out 5 samples /25g) and Salmonella enterica ser. Typhimurium monophasic (in 1 out 5 samples /25g) in chilled turkey meat from France in France

RASFF

Salmonella (presence /10g) in chilled minced beef and beef cuts from Italy in Italy

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Salmonella enterica ser. Agona (presence /25g) in organic flaxseed from Denmark in Sweden

RASFF

Salmonella (3 out of 5 samples /25g) and high count of Escherichia coli (up to 6000 CFU/g) in betel leaves from Thailand in the UK

RASFF Alert – Listeria monocytogenes – Chilled Smoked Pork Meat

European Food AlertsRASFF

Listeria monocytogenes (presence /25g) in chilled smoked pork meat from Poland in Poland

RASFF Alert – Mycotoxin -Fumonsins – Organic Corn Waffles

European Food Alerts

RASFF

fumonisins (sum B1+B2: 890; 1130 µg/kg – ppb) in organic corn waffles from Slovakia in Germany

RASFF Alerts – Ochratoxin A – Pistachios

European Food Alerts

RASFF

ochratoxin A (32.8 µg/kg – ppb) in pistachios from the United States in Switzerland

RASFF Alerts -Afaltoxin – Banku Mix – Crushed Chillies – Dried Figs – Pistachios – Groundnut Kernels

European Food Alerts

RASFF

aflatoxins (B1 = 106; Tot. = 119 µg/kg – ppb) in banku mix from Ghana in the UK

RASFF

aflatoxins (B1 = 10.3; Tot. = 11.5 µg/kg – ppb) in crushed chillies from India in Italy

RASFF

aflatoxins (B1 = 13.4; Tot. = 16.7 µg/kg – ppb) in dried figs from Turkey in Italy

RASFF

aflatoxins (Tot. = 26.3 mg/kg – ppm) in pistachios from the United States, via Turkey in Germany

RASFF

aflatoxins (B1 = 132; Tot. = 147 µg/kg – ppb) in shelled pistachios from Iran in France

RASFF

aflatoxins (B1 = 7.1; Tot. = 9.5 µg/kg – ppb) in groundnut kernels from Argentina in the Netherlands

RASFF Alerts – Animal Feed – Salmonella – Soybean Meal – Dog Chews

European Food Alerts

RASFF

Salmonella (presence /25g) in soybean meal from Germany in Germany

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Salmonella group B (in 1 out of 4 samples /25g) in dog chews from Germany in Germany

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Salmonella enterica ser. Senftenberg (presence /25g) in soybean meal from Italy in Austria