Category Archives: Microbiology Investigations

Italy – Animal Feed – HYBRID CORN – Aflatoxin

Salute

Brand : LA FATTORIA DI MZ

Name : HYBRID MAIZE zoot use KG 25 RAW MATERIAL FOR FEED

Reason for reporting : Recall due to microbiological risk

Publication date : 6 December 2021

Click to access C_17_PubblicazioneRichiami_1602_azione_itemAzione0_files_itemFiles0_fileAzione.pdf

Italy – Animal Feed – MEDIUM HYBRID CRUSHED CORN – Aflatoxin

Salute

Brand : LA FATTORIA DI MZ

Name : MEDIUM HYBRID FRANTURCO GRASS 25 KG ZOOTECHNICAL USE RAW MATERIAL FOR FEED

Reason for reporting : Recall due to microbiological risk

Publication date : 6 December 2021

Click to access C_17_PubblicazioneRichiami_1601_azione_itemAzione0_files_itemFiles0_fileAzione.pdf

Research – Bacillus amyloliquefaciens ALB65 Inhibits the Growth of Listeria monocytogenes on Cantaloupe Melons

ABSTRACT

Listeria monocytogenes is a foodborne pathogen that causes high rates of hospitalization and mortality in people infected. Contamination of fresh, ready to eat produce by this pathogen is especially troubling because of the ability of this bacterium to grow on produce under refrigeration temperatures. In this study, we created a library of over 8,000 plant phyllosphere-associated bacteria and screened them for the ability to inhibit the growth of L. monocytogenes in an in vitro fluorescence-based assay. One isolate, later identified as Bacillus amyloliquefaciens ALB65, was able to inhibit the fluorescence of L. monocytogenes by >30-fold in vitro. B. amyloliquefaciens ALB65 was also able to grow, persist, and reduce the growth of L. monocytogenes by >1.5 log CFU on cantaloupe melon rinds inoculated with 5 × 103 CFU at 30°C and was able to completely inhibit its growth at temperatures below 8°C. DNA sequence analysis of the B. amyloliquefaciens ALB65 genome revealed six gene clusters that are predicted to encode genes for antibiotic production; however, no plant or human virulence factors were identified. These data suggest that B. amyloliquefaciens ALB65 is an effective and safe biological control agent for the reduction of L. monocytogenes growth on intact cantaloupe melons and possibly other types of produce.
IMPORTANCE Listeria monocytogenes is estimated by the Centers for Disease Control and Prevention and the U.S. Food and Drug Administration to cause disease in approximately 1,600 to 2,500 people in the United States every year. The largest known outbreak of listeriosis in the United States was associated with intact cantaloupe melons in 2011, resulting in 147 hospitalizations and 33 deaths. In this study, we demonstrated that Bacillus amyloliquefaciens ALB65 is an effective biological control agent for the reduction of L. monocytogenes growth on intact cantaloupe melons under both pre- and postharvest conditions. Furthermore, we demonstrated that B. amyloliquefaciens ALB65 can completely inhibit the growth of L. monocytogenes during cold storage (<8°C).

USA – FSMA Proposed Rule on Agricultural Water

FDA

The FDA is proposing a revision to Subpart E of the FDA Food Safety Modernization Act (FSMA) Produce Safety Rule that would change the pre-harvest agricultural water requirements for covered produce (other than sprouts). The requirements in this proposed rule, if finalized, would replace the pre-harvest microbial quality criteria and testing requirements in the Produce Safety Rule with requirements for systems-based pre-harvest agricultural water assessments. These assessments would be used to identify conditions that are reasonably likely to introduce known or reasonably foreseeable hazards into or onto produce or food contact surfaces, and to determine whether corrective or mitigation measures are needed to minimize the risks associated with pre-harvest agricultural water.

These proposed requirements are intended to address stakeholder concerns about the complexity and practical implementation of certain pre-harvest agricultural water requirements in the Produce Safety Rule while continuing to protect public health. The requirements also are designed to be adaptable to future advancements in agricultural water quality science.

We are not proposing to change the requirements for harvest and post-harvest uses of agricultural water, or the agricultural water requirements for sprouts.  Sprouts are subject to specific pre-harvest agricultural water requirements, and the compliance dates for those sprouts requirements have passed.

Overview

1. Agricultural Water Assessment

The proposed rule, if finalized, would replace the pre-harvest microbial quality criteria and testing requirements in the Produce Safety Rule for covered produce (other than sprouts) with requirements for systems-based pre-harvest agricultural water assessments to be used for hazard identification and risk management decision-making (see the webpage for the final Produce Safety Rule for a description of the requirements as currently written).  Under the proposed requirements, covered farms would be required to conduct pre-harvest agricultural water assessments once annually, and whenever a change occurs that increases the likelihood that a known or reasonably foreseeable hazard will be introduced into or onto produce or food contact surfaces. As part of their pre-harvest agricultural water assessments, these farms would be required to evaluate certain factors (listed in the link above) that could impact produce safety.

Wales – Two E. coli O157 infections in children under investigation in Wales

Food Safety News

Public health officials in Wales are investigating two E. coli O157 infections at different primary schools in the country.

Public Health Wales, Conwy County Borough Council and Betsi Cadwaladr University Health Board are looking into a case of E. coli O157 at a primary school in Abergele.

The same three agencies are also investigating a probable case of E. coli O157 at a primary school in Conwy to try and find the source.

There is currently no evidence in either case to suggest the child acquired the infection at the school.

“Parents and guardians of pupils that attend the school and who may have had contact with this case have been contacted and advised on infection prevention and control measures, and what steps to take if their child develops symptoms. Contacts have been asked to submit samples for testing and to remain off school until negative results are received,” he said.

India – Karimnagar: 100 students fall sick due to food poisoning in Choppadandi

Telangana Today

Karimnagar: At least 100 students of Social Welfare Girls Residential School, Choppadandi, took ill after having lunch on Friday. They complained of stomach-ache and started vomiting following food poisoning.

According to the students, they were served cabbage, egg, dal and sambar for lunch. Immediately after lunch, they developed symptoms of food poisoning. The school authorities suspect there was a problem with the cabbage dish.

EU – Multiple EU countries part of Salmonella outbreak

Food Safety News

At least four European countries have been affected by a Salmonella outbreak with investigators as yet unable to determine the source.

Thirteen people have fallen ill in Iceland, eight in Denmark, one in Sweden and an unknown number of people were also ill in the Netherlands and Belgium. The European Centre for Disease Prevention and Control (ECDC) was informed about the outbreak by national authorities.

In September in Iceland, there was an increase in reports of Salmonella infections. Further investigations found they were the same type of Salmonella typhimurium. Samples were sent to Statens Serum Institute (SSI) in Denmark for sequencing.

Research – Bacterial Distribution and Community Structure in Beef Cattle Liver and Bile at Slaughter

Journal of Food Protection

In this study, the distribution of hygienic indicator bacteria in cattle livers and bile was examined at slaughterhouses. First, 127 cattle livers with gallbladders were carefully eviscerated from the carcasses at 10 slaughterhouses. Microbiological examination showed that 9 bile (7.1%) and 19 liver parenchyma (15.0%) samples were positive for the family Enterobacteriaceae (EB) with means ± SD of 3.68 ± 4.63 log CFU/mL and 1.59 ± 2.47 log CFU/g, respectively; thus, bacterial contamination was apparent even at the postevisceration stage. Subsequently, 70 cattle livers were obtained at the postprocessing/storage stage from 7 of the ten slaughterhouses; microbiological analysis revealed greater means of EB in the liver parenchyma (means ± SD of 3.00 ± 3.89 log CFU/g, P =0.011) than those at postevisceration stage, suggesting that bacterial dissemination and/or replication occurred in the liver parenchyma during processing and storage. According to 16S rRNA ion semiconductor sequencing analysis of representative samples from 12 cattle, Proteobacteria , Firmicutes , and Actinobacteria were dominant in both the parenchyma and bile, in which EB/ Escherichia coli were predominate among EB-rich livers. These results suggest that bile plays a role as a vehicle for bacterial transmission to the liver parenchyma. This is the first study to demonstrate bacterial distribution and community structure in the liver and biliary microecosystem of cattle at slaughter. Our data provide possible implication of EB testing in bile to screen cattle livers contaminated with high levels of fecal indicator bacteria.

Research – Prevalence of Salmonella in Chinese Food Commodities: A meta-analysis

Journal of Food Protection

kswfoodworld Salmonella

The objective of the present study was to analyze the prevalence of Salmonella in multiple food commodities in China by performing a meta-analysis. Accordingly, we screened studies that examined the prevalence of Salmonella in PubMed, Embase, and Web of Science databases. Methodological quality assessment and heterogeneity analyses were performed for included studies. The prevalence rate with the 95% confidence interval (95% CI) was selected as the effect size. Subgroup analyses for each food type were conducted and then stratified by regions, food-chain processing points, and seasons. In total, 49 studies were included in the meta-analysis, among them, 8 (16.3%) studies were deemed “High risk”, 13 (26.5%) studies were “Unclear risk”, and 28 (57.2%) studies were “Low risk”. The overall prevalence rate of Salmonella was 20.0 (95%CI: 15.9-24.4)%. The prevalence rate of Salmonella in raw meat products was 23.6 (95%CI: 19.8-27.6)%, which was higher than that in aquatic products (13.7 [95%CI: 3.1-29.9]%), milk products (0.9 [95%CI: 0.0-3.9]%), frozen convenience foods (6.5 [95%CI: 4.4-8.9]%), ready-to-eat foods (2.0 [95%CI: 1.1-3.2]%), vegetables and fruits (0.9 [95%CI: 0.0-5.2]%), and shell eggs (4.2 [95%CI: 3.0-5.7]%). Subgroup analyses revealed that prevalence rates of Salmonella in raw meat products from abattoirs (26.3 [95%CI: 17.4-36.3]%) and retail stores (30.0 [95%CI: 24.6-35.8]%) were higher than those determined from farms (10.2 [95%CI: 7.0-13.9)%; P < 0.05); however, no significant difference was observed in the prevalence of Salmonella stratified by different geographical regions or seasons (P > 0.05). Based on these findings, high levels of Salmonella contamination could be detected in raw meat products in China, and the prevalence rate of Salmonella in raw meat products from abattoirs and retail stores was high.

Research – High-Pressure-Induced Sublethal Injuries of Food Pathogens—Microscopic Assessment

MDPI

High Hydrostatic Pressure (HHP) technology is considered an alternative method of food preservation. Nevertheless, the current dogma is that HHP might be insufficient to preserve food lastingly against some pathogens. Incompletely damaged cells can resuscitate under favorable conditions, and they may proliferate in food during storage. This study was undertaken to characterize the extent of sublethal injuries induced by HHP (300–500 MPa) on Escherichia coli and Listeria innocua strains. The morphological changes were evaluated using microscopy methods such as Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Epifluorescence Microscopy (EFM). The overall assessment of the physiological state of tested bacteria through TEM and SEM showed that the action of pressure on the structure of the bacterial membrane was almost minor or unnoticeable, beyond the L. innocua wild-type strain. However, alterations were observed in subcellular structures such as the cytoplasm and nucleoid for both L. innocua and E. coli strains. More significant changes after the HHP of internal structures were reported in the case of wild-type strains isolated from raw juice. Extreme condensation of the cytoplasm was observed, while the outline of cells was intact. The percentage ratio between alive and injured cells in the population was assessed by fluorescent microscopy. The results of HHP-treated samples showed a heterogeneous population, and red cell aggregates were observed. The percentage ratio of live and dead cells (L/D) in the L. innocua collection strain population was higher than in the case of the wild-type strain (69%/31% and 55%/45%, respectively). In turn, E. coli populations were characterized with a similar L/D ratio. Half of the cells in the populations were distinguished as visibly fluorescing red. The results obtained in this study confirmed sublethal HHP reaction on pathogens cells. View Full-Text