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Category Archives: microbial contamination
Research – Occurrence and Characteristics of Escherichia albertii in Wild Birds and Poultry Flocks in Switzerland
Escherichia albertii, a zoonotic pathogen, has sporadically been associated with infectious diarrhea in humans. Poultry and wild birds are considered potential reservoirs. We assessed the occurrence of E. albertii in 280 fecal samples from wild birds (n = 130) and pooled fecal samples collected at slaughterhouse level from poultry flocks (n = 150) in Switzerland. Using an E. albertii-specific PCR targeting the Eacdt gene, 23.8% (31/130) of the samples from wild birds, but not from the pooled poultry fecal samples, tested positive for Eacdt. The positive samples originated from 11 bird species belonging to eight families. Strain isolation was attempted on the PCR-positive samples by subculturing the broth cultures onto xylose–MacConkey plates. Isolation was possible on 12 of the 31 Eacdt-PCR-positive samples. Whole-genome sequencing revealed that the strains belonged to nine distinct sequence types, with ST13420 and ST5967 being represented by two and three isolates, respectively. All strains harbored the eae gene, while two strains were also positive for stx2f. Our study thus shows that E. albertii is present in the Swiss wild bird population, which can potentially act as a source of this pathogen to humans, other animals, and the environment. View Full-Text
Research – Mathematical modeling of Bacillus cereus in Saengsik, a powdered ready-to-eat food and its application in quantitative microbial risk assessment
Abstract
In this study, we developed a mathematical model to describe the fate of Bacillus cereus in Saengsik, a powdered ready-to-eat food with reduced moisture content, and to estimate the probability of B. cereus infection from Saengsik consumption, using quantitative microbial risk assessment (QMRA). The isothermal kinetic behavior of B. cereus was analyzed as a function of temperature (5–40°C). Bacterial cell counts were fitted to the Weibull model using GinaFit, and the obtained delta values (required time for the first decimal reduction) for each temperature were 128.3–17,124.1 hr and concave (ρ < 1) were observed under all experimental conditions. The obtained delta values were analyzed using the Davey model as a function of temperature, and the performance of the developed model of survival of B. cereus was appropriate based on the validation parameters (Bf, Af, and RMSE) within the acceptable range. QMRA model was developed using data on the prevalence and concentration of B. cereus along with time and temperature along the retail-to-consumer steps. Using a baseline model with the currently available data, the probability of B. cereus infection was zero. These results provide useful information for the risk assessment and management of microbial risk in foods, especially those with very low-moisture content.
Greece – Two Clostridium botulinum Cases
The EODY rang the bell for two serious cases of botulism in Greece.
As he states in his announcement, on November 15, 2022, a confluence of 2 suspected botulism cases linked to each other by a family relationship (couple) was declared through the EODY’s Mandatory Disease Notification System.
The botulism antitoxin distribution mechanism was immediately mobilized through the EODY, in collaboration with the treating doctors, to administer the antitoxin to the two patients.
With the assistance of the World Health Organization, it was possible to administer the special antitoxin within the first twenty-four hours from the declaration of the 2 cases to EODY. The two patients, one of whom is intubated, are hospitalized in a stable clinical condition in health units in Attica.
According to the EODY, from the reported food consumption history, it emerged that the couple, of foreign nationality, who were in Greece on vacation, consumed, a few days before the onset of symptoms, asparagus prepared by them through the canning process.
It is noted that in the period 2004-2021, three laboratory-confirmed cases of botulism in infants and one suspected case in an adult were reported to EODY.
Sweden – At least 100 ill in Swedish Cryptosporidium salad outbreak
A rise in Cryptosporidium infections in Sweden has been attributed to contaminated salad by public health officials.
The Public Health Agency of Sweden (Folkhälsomyndigheten) said 101 cases of a certain type of Cryptosporidium parvum had been confirmed in 13 regions of the country.
Patients fell ill from Sept. 25 to Oct. 15 this year and women were more affected than men. Cases range from 4 to 86 years old with an average age of 42.
Link to salad
The agency added there are another 99 potential cases being investigated. The type of Cryptosporidium parvum involved is common so there could be several possible sources.
Local infection control units, the Swedish Food Agency (Livsmedelsverket) and Folkhälsomyndigheten investigated the increase in infections to find a source.
Analysis of information from patients on what they had eaten before becoming ill shows they ate mixed or bagged salad more often than a control group.
Salad has a short shelf life so health officials don’t think it is still available for sale but no products have been sampled.
Livsmedelsverket has looked into possible producers and growers but has been unable to find the likely source of infection.
As reported cases of Cryptosporidium have decreased and are at similar levels to previous years, officials believe the outbreak is over.
Posted in Contaminated water, Cryptosporidiosis, Cryptosporidium, food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak, Water, water microbiology, Water Safety
Hong Kong – Draft ‘Oysters Intended for Raw Consumption – the Food Safety Guidelines for Food Businesses’
The Centre for Food Safety has drafted a set of trade guidelines on oysters intended for raw consumption. This set of guidelines aims to assist the food businesses in understanding the risks associated with raw oysters and implementing appropriate and practical food safety measures in their operations to reduce the occurrence of foodborne illnesses while also allowing susceptible groups of consumers to make informed choices. We are inviting members of the trade to provide comments on the draft guidelines. Your valuable comments will help us improve this set of guides. Please return your comments by 30 November 2022. Thank you.
Draft Oysters Intended for Raw Consumption – Food Safety Guidelines for Food Businesses
USA – FDA – Core Outbreak Table Update
What’s New
- A new outbreak of Salmonella Typhimurium (reference #1113) in a not yet identified food has been added to the table and traceback has been initiated. There are currently 264 cases. The FDA is working closely with federal, state, and local partners to investigate multiple food items of interest to determine the source of this outbreak and will update the public when more information becomes available.
- For the E. coli O157:H7 outbreak in a not yet identified food (reference #1121), the outbreak status has changed to “Ended”.
Posted in E.coli O157, E.coli O157:H7, FDA, food bourne outbreak, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Poisoning, foodborne outbreak, foodbourne outbreak, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak, Salmonella
Norovirus: What to do if you catch it and helping to stop the spread
Norovirus, commonly known as the winter vomiting bug, is a stomach bug that causes sickness and diarrhoea. Following lower levels than normal throughout the COVID-19 pandemic, we have started to see an increase in norovirus activity as we head into the winter period.
Norovirus is able to spread easily through communities and so outbreaks are common in settings where individuals have close contact such as hospitals, care homes, schools and nurseries.
For most people this is an unpleasant, short-lived illness and they make a full recovery within 2-3 days without needing any medicine. However, some groups, including young children, the elderly or those with weakened immunity, are at risk of suffering more serious and prolonged illness which may require medical treatment.
Stopping the spread
Norovirus is easily transmitted through contact with people with the infection and any surfaces or objects which have been contaminated with the virus. Symptoms include sudden onset of nausea, projectile vomiting and diarrhoea but can also include a high temperature, abdominal pain and aching limbs.
The incubation period of norovirus is 12-48 hours, which is the time between catching the virus and developing symptoms. Individuals are most infectious when symptomatic, but it is possible to pass on norovirus or shed the virus, thereby contaminating surfaces, objects or even food, both before developing symptoms and after symptoms have stopped.
Good hand hygiene is important to stop norovirus spreading. To avoid catching norovirus or passing it on to others wash your hands frequently and thoroughly with soap and warm water. This is most important following an episode of illness, after using the toilet, before eating or preparing food as well as cleaning up vomit or diarrhoea. Alcohol-based hand sanitisers are not effective against norovirus.

RASFF Alerts – Animal Feed – Salmonella – Rapeseed Extract – Meat Product
Salmonella in post-extraction rapeseed meal from Ukraine in Poland and Lithuania
Salmonella in meat product from Belgium in France
Research – Prevalence and Implications of Shiga Toxin-Producing E. coli in Farm and Wild Ruminants

Abstract
Shiga-toxin-producing Escherichia coli (STEC) is a food-borne pathogen that causes human gastrointestinal infections across the globe, leading to kidney failure or even death in severe cases. E. coli are commensal members of humans and animals’ (cattle, bison, and pigs) guts, however, may acquire Shiga-toxin-encoded phages. This acquisition or colonization by STEC may lead to dysbiosis in the intestinal microbial community of the host. Wildlife and livestock animals can be asymptomatically colonized by STEC, leading to pathogen shedding and transmission. Furthermore, there has been a steady uptick in new STEC variants representing various serotypes. These, along with hybrids of other pathogenic E. coli (UPEC and ExPEC), are of serious concern, especially when they possess enhanced antimicrobial resistance, biofilm formation, etc. Recent studies have reported these in the livestock and food industry with minimal focus on wildlife. Disturbed natural habitats and changing climates are increasingly creating wildlife reservoirs of these pathogens, leading to a rise in zoonotic infections. Therefore, this review comprehensively surveyed studies on STEC prevalence in livestock and wildlife hosts. We further present important microbial and environmental factors contributing to STEC spread as well as infections. Finally, we delve into potential strategies for limiting STEC shedding and transmission. View Full-Text
Research – Low levels of E. coli found in Norwegian produce testing
Testing of fresh produce in Norway has found a low level of E. coli contamination.
The Norwegian Food Safety Authority (Mattilsynet) commissioned the Veterinary Institute to examine ready-to-eat lettuce and sugar snap peas in 2021.
A total of 118 samples, of which 37 were sugar snap peas and 81 ready-to-eat leafy greens, were analyzed. Salmonella was not detected, while E. coli was found in 11 samples, one of sugar snap peas and 10 of lettuce. E. coli, as a hygiene indicator, at levels above the guideline values was only detected in one sample of lettuce.
Results were considered unsatisfactory if three or more samples contained more than 100 colony forming units per gram or one sample contained above 1,000 CFU/g.
Officials said finding high levels of E. coli in lettuce may indicate the production process is not working optimally. Good raw material control and management of the washing and rinsing process are important, so microorganisms do not build-up and cross-contamination via water is minimized.
Posted in Decontamination Microbial, E.coli, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Salmonella



