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Category Archives: Decontamination Microbial
Research – Microbial Succession in the Cheese Ripening Process—Competition of the Starter Cultures and the Microbiota of the Cheese Plant Environment
A large variety of cheeses can be produced using different manufacturing processes and various starter or adjunct cultures. In this study, we have described the succession of the microbial population during the commercial production and subsequent ripening of smear-ripened cheese using 16S rRNA gene sequencing. The composition of the microbiota during the first 6 days of production was constant and consisted mainly of LAB (lactic acid bacteria) originating from the starter culture. From day 7, the proportion of LAB decreased as other bacteria from the production environment appeared. From the 14th day of production, the relative proportion of LAB decreased further, and at the end of ripening, bacteria from the environment wholly dominated. These adventitious microbiota included Psychrobacter, Pseudoalteromonas haloplanktis/hodoensis, Vibrio toranzoniae, and Vibrio litoralis (Proteobacteria phylum), as well as Vagococcus and Marinilactibacillus (Firmicutes phylum), Psychrilyobacter (Fusobacteria phylum), and Malaciobacter marinus (Campylobacterota phylum), all of which appeared to be characteristic taxa associated with the cheese rind. Subsequent analysis showed that the production and ripening of smear-ripened cheese could be divided into three stages, and that the microbiota compositions of samples from the first week of production, the second week of production, and supermarket shelf life all differed.
Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, lactic acid bacteria, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk
Research – Assessing the Risk of Seasonal Effects of Campylobacter Contaminated Broiler Meat Prepared In-Home in the United States
Abstract
Campylobacter has consistently posed a food safety issue in broiler meat. This study aimed to create a quantitative microbial risk assessment model from retail to consumption, designed to evaluate the seasonal risk of campylobacteriosis associated with broiler meat consumption in the United States. To achieve this, data was gathered to build distributions that would enable us to predict the growth of Campylobacter during various stages such as retail storage, transit, and home storage. The model also included potential fluctuations in concentration during food preparation and potential cross-contamination scenarios. A Monte Carlo simulation with 100,000 iterations was used to estimate the risk of infection per serving and the number of infections in the United States by season. In the summer, chicken meat was estimated to have a median risk of infection per serving of 9.22 × 10−7 and cause an average of about 27,058,680 infections. During the winter months, the median risk of infection per serving was estimated to be 4.06 × 10−7 and cause an average of about 12,085,638 infections. The risk assessment model provides information about the risk of broiler meat to public health by season. These results will help understand the most important steps to reduce the food safety risks from contaminated chicken products.
Posted in Campylobacter, campylobacter coli, Campylobacter jejuni, Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk
Research – Use of Essential Oil Emulsions to Control Escherichia coli O157:H7 in the Postharvest Washing of Lettuce
Abstract
Essential oils (EOs) have strong antibacterial properties and can be potential sanitizers to reduce pathogen load and prevent cross-contamination during postharvest washing. The objective of this study was to investigate the efficacy of emulsions containing oregano (OR; Origanum vulgare) and winter savory (WS; Satureja montana) EOs at different concentrations (0.94 and 1.88 µL/mL) and storage times (0 h, 24 h, and 7 days), in reducing Escherichia coli O157:H7 on the surface of three types of lettuce (romaine, crisphead, and butterhead). The EO emulsions were compared with one no-rinse treatment and three rinse treatments using water, 200 ppm chlorine, and 80 ppm peroxyacetic acid (PAA), respectively, in a simulated washing system. The results showed that while the EO emulsions significantly reduced E. coli O157:H7 on crisphead lettuce over time, not all treatments were effective for romaine and butterhead lettuce. The mixture of OR and WS at concentrations of 0.94 and 1.88 µL/mL was found to be the most effective in reducing E. coli O157:H7 on inoculated lettuce, resulting in reductions of 3.52 and 3.41 log CFU/g, respectively. Furthermore, the PAA and the mixture of OR and WS at 1.88 µL/mL effectively limited bacterial cross-contamination close to the detection limit for all lettuce types during all storage times. These results suggest that OR and WS EOs could serve as potential alternatives to chemical sanitizers for postharvest lettuce washing.
Posted in Antimicrobials, Decontamination Microbial, E.coli, E.coli O157, E.coli O157:H7, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk
USA- CDC – Notes from the Field: Doubling of Cyclosporiasis Cases Partially Attributable to a Salad Kit — Florida, 2021–2022
Cyclosporiasis is a gastrointestinal infection caused by a protozoan parasite, Cyclospora cayetanensis. This species is only known to infect humans and is acquired when oocysts are ingested through food or water contaminated with feces that contain the parasite. The illness was first reported in 1979, and the organism was identified and named in 1994 (1). Historically, infections were typically acquired outside of the United States or from produce that was imported into the United States (1). In recent years, the number of reported U.S. cases has been increasing: cases more than doubled from 537 in 2016 to 1,194 in 2017, and then nearly tripled, to 3,519 cases in 2018; in 2019, 4,703 cyclosporiasis cases were reported.* Recently, the parasite has been found on domestically grown produce (2), and infections have been attributed to these foods (3). Produce washing will decrease but not eliminate the parasite (1).
Posted in Cyclospora, Cyclosporiasis, Decontamination Microbial, food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak
Quebec- Notice not to consume the Pam Pi Gou brand 100% Haitian seasoning mix sold by the company Épicerie antillais et casse-croute Ében-Ézer – Clostridium botulinum
QUEBEC CITY , July 6. 2, 2023 /CNW/ – The Ministère de l’Agriculture, des Pêcheries et de l’Alimentation (MAPAQ), in collaboration with Épicerie antillais et casse-croute Ében-Ézer, located at 125, rue Valmont, in Repentigny , advises the population not to consume the product indicated in the table below, because it has not been prepared and packaged in such a way as to ensure its harmlessness. Indeed, this product could promote the proliferation of the bacterium Clostridium botulinum .
|
Product name |
Format |
Affected lot |
|
“100% HAITIAN SEASONING” |
500ml _ |
Units sold until July 5, 2023 |
The product that is the subject of this warning was offered for sale until July 5, 2023, and only at the place designated above. The product was packaged in a Mason type glass jar and sold chilled. The product label includes, in addition to its name, the words “PAM PI GOU”.
The operator is voluntarily recalling the product in question. It has agreed with MAPAQ to issue this warning as a precautionary measure. In addition, people who have this product in their possession are advised not to consume it. They must return it to the establishment where they bought it or throw it away.
Even if the affected product shows no sign of tampering or suspicious odor, it could be contaminated with Clostridium botulinum bacteria . Foods contaminated with the toxin of this bacterium can cause, when ingested, the following symptoms: facial paralysis, non-reactive or fixed pupils, difficulty swallowing, droopy eyelids, vision and speech disorders. In severe cases, the disease can cause death.
It should be noted that no case of illness associated with the consumption of this food has been reported to MAPAQ to date.
New in August on Netflix – Poisoned: The Dirty Truth About Your Food

The doc first premiered at the Tribeca Film Festival with multiple screenings taking place between June 9th and June 17th first premiering at the SVA Theatre on June 9th. It also was shown in the author, Jeff Benedict’s, hometown, as well as Bill Marler’s, the main character of the film.
Here’s what you can expect from the documentary based on the book Poisoned by Jeff Benedict (which has an updated cover to reflect the Netflix adaptation).Netflix was first announced to be attached to develop the documentary on the 2011 book back in November 2022. Here is the link and the cover:
Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Poisoning, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Poisoning
Sweden – Increase in cases of illness with Campylobacter infection
In recent weeks, there has been an increase in the number of people who have contracted campylobacter infection. The rise in the number of cases is preceded by an increase in campylobacter in broiler flocks. Good hygiene when handling raw chicken reduces the risk of becoming infected.
Figure: The number of reported cases of campylobacter per week infected in Sweden or with an unknown country of infection (230706). Week 27 is not yet complete.

From mid-June, an increase in the occurrence of campylobacter in Swedish broiler flocks has also been observed. Previous studies have shown a connection between campylobacter infection and the consumption of fresh chicken, where around a third of those who have contracted the disease have been linked to fresh chicken. Campylobacter infection in humans is more common in summer as is the presence of campylobacter in broiler flocks.
Posted in Campylobacter, campylobacter coli, Campylobacter jejuni, Decontamination Microbial, food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak
Sweden sees another rise in illnesses for 2022
Most foodborne infections rose in Sweden in 2022 compared to the year before, according to the latest figures.
A report by the National Veterinary Institute (SVA), Folkhälsomyndigheten (the Public Health Agency of Sweden), Livsmedelsverket (the Swedish Food Agency) and Jordbruksverket (Swedish Board of Agriculture) showed a rise for Campylobacter, Salmonella, Listeria, and E. coli infections. Some of this was because of increased travel and more people being infected abroad.
A total of 5,165 cases of campylobacteriosis were reported in 2022 and 57 percent of these were domestic. This is up on more than 4,000 cases in 2021. For domestic infections in 2022, the median age was 51 with a range of 0 to 101. More men, 54 percent, than women were sick.
A correlation between human cases and Campylobacter-positive broiler batches underscores the need for further preventive measures, said the report.
Posted in Campylobacter, campylobacter coli, Campylobacter jejuni, Decontamination Microbial, E.coli, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, Illness, Listeria, Listeria monocytogenes, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Salmonella
Research – Is Legionella Bacteria Becoming Antibiotic Resistant?
In this article the water safety specialists at Legionella Control International look at the role of antibiotics in protecting humans against pathogenic organisms, paying particular attention to waterborne bacteria including Pseudomonas aeruginosa and legionella.
Posted in Antibiotic Resistance, Contaminated water, Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Legionella, Legionnaires’ disease, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Water, water microbiology, Water Safety
USA – No reduction in human illnesses from poultry-caused Salmonella
“While the prevalence of Salmonella contamination in regulated poultry products has decreased by more than 50 percent in recent years, there has not been a reduction in human illnesses attributable to poultry,” a new USDA report says.
USDA’s Food Safety and Inspection Service (FSIS) has released a survey of Not-Ready-to-Eat Breaded and Stuffed Chicken Products for Salmonella. The June report was prepared by FSIS’s Laboratory Quality Assurance, Response, and Coordination (LQARCS) staff in the Office of Public Health.
According to the survey report: “FSIS worked with the Food Emergency Response Network to test for the presence of Salmonella and sanitary indicator aerobic organism counts using the current validated methods employed by 11 state public health and agriculture laboratories. From July 1, 2022, to September 30, 2022, the laboratories purchased approximately 15 samples of the product per month from nearby retail locations.
Posted in Decontamination Microbial, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Salmonella, Salmonella in Chicken


