Category Archives: Research

Research – Spanish study finds organic produce contamination

Food Safety News

Early findings from researchers in Spain has found organic produce can be contaminated with a range of bacteria.

The study suggests that amoebas that live on organic leafy vegetables can shelter human pathogens like Pseudomonas, Salmonella, and Helicobacter.

Details are based on a poster presentation at the European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) in Lisbon, Portugal, from April 23 to 26. All abstracts were peer reviewed by a congress committee. There is no paper yet, but the research has been submitted to a medical journal for publication.

However, other scientists warned that while conclusions are interesting, the fact that data has not yet been peer reviewed means they cannot be put into context.

Research – An Evaluation of Food Safety Performance in Wineries

MDPI

Wine production has food safety hazards. A Hazard Analysis Critical Control Point (HACCP) system makes it possible to identify, evaluate, and control significant food safety hazards throughout the wine production process. The Prerequisites Programs (PPRs) and HACCP performance in Protected Denomination of Origin “Vinos de Madrid” wineries were analyzed. Winery performances were evaluated for every critical control point (CCPs) in each winemaking process stage, including their implementation of PPR and HACCP principles. This study was developed through a survey of 55 questions divided into 11 sections, and it was conducted on a sample of 21 wineries. The results revealed that the CCPs worst performance level are for the control of metals (Cd, Pb, As) in grapes and fungicides or pesticide control in the harvest reception. A total of 91.5% of the wineries had implemented a prerequisites program (PPRs), regardless of their annual wine production. However, there was variability in the type of prerequisite plans, training, level of knowledge of operators, and annual budget allocation. Three out of four wineries had an HACCP, although corrective action procedures and verification procedures had the lowest and the worst HACCP practical implementation. The significant barriers for HACCP performance in wineries are linked with a lack of food safety staff training, low involvement of all staff in food safety tasks, and poor application of CCP chemical and microbiologic control methods.

Research – Role of Lactic Acid Bacteria in Food Preservation and Safety

MDPI

Fermentation of various food stuffs by lactic acid bacteria is one of the oldest forms of food biopreservation. Bacterial antagonism has been recognized for over a century, but in recent years, this phenomenon has received more scientific attention, particularly in the use of various strains of lactic acid bacteria (LAB). Certain strains of LAB demonstrated antimicrobial activity against foodborne pathogens, including bacteria, yeast and filamentous fungi. Furthermore, in recent years, many authors proved that lactic acid bacteria have the ability to neutralize mycotoxin produced by the last group. Antimicrobial activity of lactic acid bacteria is mainly based on the production of metabolites such as lactic acid, organic acids, hydroperoxide and bacteriocins. In addition, some research suggests other mechanisms of antimicrobial activity of LAB against pathogens as well as their toxic metabolites. These properties are very important because of the future possibility to exchange chemical and physical methods of preservation with a biological method based on the lactic acid bacteria and their metabolites. Biopreservation is defined as the extension of shelf life and the increase in food safety by use of controlled microorganisms or their metabolites. This biological method may determine the alternative for the usage of chemical preservatives. In this study, the possibilities of the use of lactic acid bacteria against foodborne pathogens is provided. Our aim is to yield knowledge about lactic acid fermentation and the activity of lactic acid bacteria against pathogenic microorganisms. In addition, we would like to introduce actual information about health aspects associated with the consumption of fermented products, including probiotics.

Research – Mechanisms of Inhibition of Quorum Sensing as an Alternative for the Control of E. coli and Salmonella

MDPI

Quorum sensing (QS) is a process of cell–cell communication for bacteria such as E. coli and Salmonella that cause foodborne diseases, with the production, release, and detection of autoinducer (AI) molecules that participate in the regulation of virulence genes. All of these proteins are useful in coordinating collective behavior, the expression of virulence factors, and the pathogenicity of Gram-negative bacteria. In this work, we review the natural or synthetic inhibitor molecules of QS that inactivate the autoinducer and block QS regulatory proteins in E. coli and Salmonella. Furthermore, we describe mechanisms of QS inhibitors (QSIs) that act as competitive inhibitors, being a useful tool for preventing virulence gene expression through the downregulation of AI-2 production pathways and the disruption of signal uptake. In addition, we showed that QSIs have negative regulatory activity of genes related to bacterial biofilm formation on clinical artifacts, which confirms the therapeutic potential of QSIs in the control of infectious pathogens. Finally, we discuss resistance to QSIs, the design of next-generation QSIs, and how these molecules can be leveraged to provide a new antivirulence therapy to combat diseases caused by E. coli or SalmonellaView Full-Text

Research – Unraveling the Antimicrobial Effectiveness of Coridothymus capitatus Hydrolate against Listeria monocytogenes in Environmental Conditions Encountered in Foods: An In Vitro Study

MDPI

The increased resistance of bacteria to antimicrobials, as well as the growing interest in innovative and sustainable alternatives to traditional food additives, are driving research towards the use of natural food preservatives. Among these, hydrolates (HYs) have gained attention as “mild” alternatives to conventional antimicrobial compounds. In this study, the response of L. monocytogenes ATCC 7644 exposed to increasing concentrations of Coridothymus capitatus HY (CHY) for 1 h at 37 °C was evaluated by means of Phenotype Microarray, modelling the kinetic data obtained by inoculating control and treated cells into GEN III microplates, after CHY removal. The results revealed differences concerning the growth dynamics in environmental conditions commonly encountered in food processing environments (different carbon sources, pH 6.0, pH 5.0, 1–8% NaCl). More specifically, for treated cells, the lag phase was extended, the growth rate was slowed down and, in most cases, the maximum concentration was diminished, suggesting the persistence of stress even after CHY removal. Confocal Laser Scanner Microscopy evidenced a diffuse aggregation and suffering of the treated cells, as a response to the stress encountered. In conclusion, the treatment with HY caused a stressing effect that persisted after its removal. The results suggest the potential of CHY application to control L. monocytogenes in food environments.

Israel – How did salmonella spread in the Strauss production facility? – analysis

Jpost

How does salmonella spread in a food production facility and can it be prevented?

Because salmonella is a pathogen that is prevalent in the gut of many animals and insects, it can spread by the animal’s feces into the soil, water and food. From there it can easily be introduced into and spread throughout a food production facility by way of raw ingredients, as was the case with the Strauss facility. It can also spread through the facility via contaminated packaging, equipment and workers’ hands and clothing.

Salmonella thrives in moist, warm environments such as drains, floors and processing equipment, making a food production facility the perfect environment for it.

However, there are several ways to ensure that the risk of salmonella contamination in a food facility is lessened, according to international cleaning equipment supplier Vikan’s hygiene and compliance manager Stine Lønnerup Bislev.

The first step to controlling the spread of the bacteria is to ensure that only clean and disinfected equipment, tools and utensils are brought into the processing area, Bislev explains on the company’s website. Then, all incoming ingredients and materials that pose a risk for contamination should be tested for salmonella ahead of production.

Ingredients must be cleaned and disinfected properly, using either a wet or dry cleaning method, depending on the moisture content of the ingredient. High moisture ingredients that require wet cleaning include meat, vegetables and dairy products, Bislev notes.

To avoid the spread of contamination, raw and processed foods must be handled in separate areas, using separate tools and equipment. Cleaning equipment should meet the appropriate hygiene standards to facilitate easy cleaning and prevent microbial growth.

Hygenic design features include smooth surfaces, one-piece construction, easy dismantling and a lack of crevices.

Finally, Bislev states, a program should be developed by the production facility’s management in order to routinely sample the space for salmonella.

“An environmental monitoring program will assess the effectiveness of your overall hygienic practices, monitor the environment for transient pathogens and help mitigate potential harborage and growth niches,” she concludes.

Read more at the link above.

USA – Publisher’s Platform: Hey Chicken Little, the sky will not fall if Salmonella is deemed an adulterant

LEX

If Salmonella is deemed an adulterant – at least those that sicken and kill us – the sky will not fall – history as a guide.

On Jan. 19, 2020, we filed a petition with USDA’s Food Safety and Inspection Service (FSIS), on behalf of Rick Schiller, Steven Romes, the Porter family, Food & Water Watch, Consumer Federation of America, and Consumer Reports. 20-01-marler-011920 The petition asked FSIS to declare the following Salmonella “outbreak serotypes” as per se contaminants (adulterants) in meat and poultry products:

Salmonella Agona, Anatum, Berta, Blockely, Braenderup, Derby, Dublin, Enteritidis, Hadar, Heidelberg, I 4,[5],12:i:-, Infantis, Javiana, Litchfield, Mbandaka, Mississippi, Montevideo, Muenchen, Newport, Oranienburg, Panama, Poona, Reading, Saintpaul, Sandiego, Schwarzengrund, Senftenberg, Stanley, Thompson, Typhi, and Typhimurium.

I said at the time, reducing salmonellosis from meat and poultry “demands bold action” beyond that yet taken by FSIS. Salmonella is a leading cause of foodborne illness in the United States, causing 1.35 million illnesses, 26,500 hospitalizations, 130 outbreaks, and 420 deaths each year.

Presently, government regulators are somewhat silent with what they intend to do.  The poultry industry, as expected, sees any additional regulation as unnecessary, burdensome and costly.

Read More at the link above.

Germany -Officials admit failures in German Listeria outbreak

Food Safety News

Officials in a German district have apologized for mistakes made as part of an investigation into a Listeria outbreak in which one person died.

Four people were infected between October 2021 and January this year but the person died with, not because of, listeriosis. Contaminated cucumbers from one company have been linked to the illnesses.

Thomas Will, Groß-Gerau district administrator and Walter Astheimer, district health officer, said in the past two and a half years, there have been significant gaps in food controls and this had been a big mistake with hygiene deficiencies overlooked.

“We deeply regret that food contaminated with Listeria could come into circulation and several people fell ill because a company in Gernsheim was not properly checked for two years,” they said.

The company should have been checked more often but inspections were running behind because of the COVID-19 pandemic and a lack of staff resources.

Research – Observational Study of the Impact of a Food Safety Intervention on Consumer Poultry Washing

Journal of Food Protection

This study was conducted to test the effectiveness of a consumer poultry washing educational intervention that included video observation of meal preparation with participants who self-reported washing poultry. Treatment group participants received three e-mail messages containing information that the U.S. Department of Agriculture has used on social media sites (video and infographics) related to poultry preparation, including advising against washing chicken. Participants were observed cooking chicken thighs (inoculated with traceable nonpathogenic Escherichia coli strain DH5α) and preparing a salad to determine whether they washed the chicken and the extent of cross-contamination to the salad and areas of the kitchen. After meal preparation, participants responded to an interview about food handling behaviors, including questions about the intervention for treatment group participants. Three hundred people participated in the study (158 control, 142 treatment). The intervention effectively encouraged participants not to wash chicken before cooking; 93% of treatment group participants but only 39% of control group participants did not wash the chicken (P < 0.0001). The high levels of E. coli DH5α detected in the sink and on the salad lettuce suggest that microbes transferred to the sink from the chicken, packaging, or contaminated hands are a larger cause for concern than is splashing contaminated chicken fluids onto the counter. Among chicken washers, 26 and 30% of the lettuce from the prepared salad was contaminated for the control and treatment groups, respectively. For nonwashers, 31 and 15% of the lettuce was contaminated for the control and treatment groups, respectively. Hand-facilitated cross-contamination is suspected to be a factor in explaining this resulting lettuce cross-contamination. This study demonstrates the need to change the frame of “don’t wash your poultry” messaging to instead focus on preventing contamination of sinks and continuing to emphasize the importance of hand washing and cleaning and sanitizing surfaces.

HIGHLIGHTS
  • The intervention was effective for convincing participants to not wash chicken before cooking.
  • High levels of the bacterial tracer were detected in the sink and on the salad lettuce.
  • Messaging should focus on hand washing and cleaning and sanitizing surfaces.

Research – Innovative Antibiofilm Smart Surface against Legionella for Water Systems

MDPI

Legionella pneumophila contamination of water systems is a crucial issue for public health. The pathogen is able to persist in water as free-living planktonic bacteria or to grow within biofilms that adhere to and clog filters and pipes in a water system, reducing its lifespan and, in the case of hospital buildings, increasing the risk of nosocomial infections. The implementation of water management is considered to be the main prevention measure and can be achieved from the optimization of water system architecture, notably introducing new materials and strategies to contrast Legionella biofilm proliferation and so prolong the water system functionality. In this research, we propose a new smart surface against L. pneumophila biofilm formation. This is based on an innovative type of coating consisting of a sulfonated pentablock copolymer (s-PBC, commercially named Nexar™) deposited on top of a polypropylene (PP) coupon in a sandwich filter model. The covering of PP with s-PBC results in a more hydrophilic, acid, and negatively charged surface that induces microbial physiological inhibition thereby preventing adhesion and/or proliferation attempts of L. pneumophila prior to the biofilm formation. The antibiofilm property has been investigated by a Zone of Inhibition test and an in vitro biofilm formation analysis. Filtration tests have been performed as representative of possible applications for s-PBC coating. Results are reported and discussed.