Category Archives: Lactobacillus plantarum

Research – Evaluation of the safety and efficacy of lactic acid to reduce microbiological surface contamination on carcases from kangaroos, wild pigs, goats and sheep

EFSA

Studies evaluating the safety and efficacy of lactic acid to reduce microbiological surface contamination from carcases of wild game (i.e. kangaroos and wild pigs) and small stock (i.e. goats and sheep) before chilling at the slaughterhouse were assessed. Wild pig and kangaroo hide‐on carcases may have been chilled before they arrive at the slaughterhouse and are treated after removal of the hides. Lactic acid solutions (2–5%) are applied to the carcases at temperatures of up to 55°C by spraying or misting. The treatment lasts 6–7 s per carcass side. The Panel concluded that: [1] the treatment is of no safety concern, provided that the lactic acid complies with the European Union specifications for food additives; [2] based on the available evidence, it was not possible to conclude on the efficacy of spraying or misting lactic acid on kangaroo, wild pig, goats and sheep carcases; [3] treatment of the above‐mentioned carcases with lactic acid may induce reduced susceptibility to the same substance, but this can be minimised; there is currently no evidence that prior exposure of food‐borne pathogens to lactic acid leads to the occurrence of resistance levels that compromise antimicrobial therapy; and [4] the release of lactic acid is not of concern for the environment, assuming that wastewaters released by the slaughterhouses are treated on‐site, if necessary, to counter the potentially low pH caused by lactic acid, in compliance with local rules.

Research – Role of Lactic Acid Bacteria in Food Preservation and Safety

MDPI

Fermentation of various food stuffs by lactic acid bacteria is one of the oldest forms of food biopreservation. Bacterial antagonism has been recognized for over a century, but in recent years, this phenomenon has received more scientific attention, particularly in the use of various strains of lactic acid bacteria (LAB). Certain strains of LAB demonstrated antimicrobial activity against foodborne pathogens, including bacteria, yeast and filamentous fungi. Furthermore, in recent years, many authors proved that lactic acid bacteria have the ability to neutralize mycotoxin produced by the last group. Antimicrobial activity of lactic acid bacteria is mainly based on the production of metabolites such as lactic acid, organic acids, hydroperoxide and bacteriocins. In addition, some research suggests other mechanisms of antimicrobial activity of LAB against pathogens as well as their toxic metabolites. These properties are very important because of the future possibility to exchange chemical and physical methods of preservation with a biological method based on the lactic acid bacteria and their metabolites. Biopreservation is defined as the extension of shelf life and the increase in food safety by use of controlled microorganisms or their metabolites. This biological method may determine the alternative for the usage of chemical preservatives. In this study, the possibilities of the use of lactic acid bacteria against foodborne pathogens is provided. Our aim is to yield knowledge about lactic acid fermentation and the activity of lactic acid bacteria against pathogenic microorganisms. In addition, we would like to introduce actual information about health aspects associated with the consumption of fermented products, including probiotics.

Research – Lactic Acid Bacteria as Antimicrobial Agents: Food Safety and Microbial Food Spoilage Prevention

MDPI

In the wake of continual foodborne disease outbreaks in recent years, it is critical to focus on strategies that protect public health and reduce the incidence of foodborne pathogens and spoilage microorganisms. Currently, there are limitations associated with conventional microbial control methods, such as the use of chemical preservatives and heat treatments. For example, such conventional treatments adversely impact the sensorial properties of food, resulting in undesirable organoleptic characteristics. Moreover, the growing consumer advocacy for safe and healthy food products, and the resultant paradigm shift toward clean labels, have caused an increased interest in natural and effective antimicrobial alternatives. For instance, natural antimicrobial elements synthesized by lactic acid bacteria (LAB) are generally inhibitory to pathogens and significantly impede the action of food spoilage organisms. Bacteriocins and other LAB metabolites have been commercially exploited for their antimicrobial properties and used in many applications in the dairy industry to prevent the growth of undesirable microorganisms. In this review, we summarized the natural antimicrobial compounds produced by LAB, with a specific focus on the mechanisms of action and applications for microbial food spoilage prevention and disease control. In addition, we provide support in the review for our recommendation for the application of LAB as a potential alternative antimicrobial strategy for addressing the challenges posed by antibiotic resistance among pathogens. View Full-Text

Research – Anti‐adhesive effects of sialic acid and Lactobacillus plantarum on Staphylococcus aureus in vitro

Journal of Food Safety

Staphylococcus aureus (S. aureus) is a common food‐borne pathogen that causes severe diseases after adhesion to epithelial cells. Lactobacillus inhibits pathogenic bacterial adhesion and infection. In addition, sialic acid (SA) is widely known for its beneficial biological functions. A new way of reducing the occurrence of diseases and curbing the overuse of antibiotics is ingesting prebiotics and probiotics that regulate the intestinal flora. In this study, we first evaluated the anti‐adhesive effects of several strains of Lactobacillus on S. aureus. The study revealed that the S. aureus adhesion was inhibited by all the strains of Lactobacillus. Besides, the rate of inhibition by L. plantarum Z‐4 was significantly higher than other Lactobacillus species. We then investigated the effects of different SA concentrations (40, 100, 150, 200, and 260 μg/ml) on the growth and adhesion characteristics of L. plantarum and S. aureus. The results showed that SA influences bacterial adhesion by regulating the bacteria’s growth characteristics. Finally, the effects of SA combined with Lactobacillus on the adhesion of S. aureus were assessed by competition, exclusion and displacement methods. SA with a concentration of 260 μg/mL combined with L. plantarum had the highest inhibition effect on the competition assays. In addition, the expression of S. aureus adhesion‐related genes was reduced. This provides a new perspective on the application of SA and/or L. plantarum and its potential to resist adhesion of S. aureus.

RASFF Alert- Animal Feed – unauthorised ingredients in fish feed

European Food Alerts

RASFF

unauthorised ingredients (Lactobacillus plantarum, Enterococcus faecalis, Pediococcus lactis) in fish feed from China in the UK

Research – Modeling the interactions among Salmonella enteritidis, Pseudomonas aeruginosa , and Lactobacillus plantarum

Wiley Online

This paper was to investigate the interactions among Salmonella enteritidis, Lactobacillus plantarum , and Pseudomonas aeruginosa at four combinations of initial concentration. Firstly, fitting the growth curves to obtain growth parameters—lag time (λ ), maximal growth rate ( μ max), initial concentration (0), and maximum population density (max) for each strain in monocultures or cocultures. Then interactions among S. enteritidis, P. aeruginosa , and L. plantarum in cocultures at four combinations of initial concentration were quantified by the Lotka–Volterra model with six interaction coefficients. Results indicated that there were no interactions between S. enteritidis and P. aeruginosa S. enteritidis and P. aeruginosa had an inhibitory effect on L. plantarum , but L. plantarum had no effects on another two. Besides, the higher the initial concentrations of S. enteritidis or P. aeruginosa , the lower the growth potential of L. plantarum . This study provided more accurate predictions for the growth of bacteria under actual food contamination conditions.

Research – Interaction and inactivation of Listeria and Lactobacillus cells in single and mixed species biofilms exposed to different disinfectants

Wiley Online

Abstract

Listeria spp. are ubiquitously found in both the natural and the food processing environment, of which Listeria monocytogenes is of an important health risk. Here, we report on the formation of single and mixed species biofilms of L. monocytogenes/Listeria innocua and Lactobacillus plantarum strains in 24‐well polystyrene microtiter plates and on the inactivation of 24‐hr and 72‐hr biofilms using quaternary ammonium compound‐, tertiary alkyl amine‐, and chlorine‐based disinfectants. Fluorescent in situ hybridization (FISH) and LIVE/DEAD BacLight staining were applied for 72‐hr L. innocuaL. plantarum mixed biofilms in the LabTek system for the species identification and the reaction of biofilm cells to disinfectants, respectively. L. monocytogenes/L. innocua were more resistant to disinfectants in 72‐hr than in 24‐hr biofilms, whereas L. plantarum strains did not show any significant differences between 72‐hr and 24‐hr biofilms. Furthermore, L. innocua when grown with L. plantarum was more resistant to all disinfection treatments, indicating a protective effect from lactobacilli in the mixed species biofilm. The biofilm formation and reaction to disinfectants, microscopically verified using fluorescence in situ hybridization and LIVE/DEAD staining, showed that L. innocua and L. plantarum form a dense mixed biofilm and also suggested the shielding effect of L. plantarum on L. innocua in the mixed species biofilm.