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Category Archives: Microbiology
The Use of Ozone Technology: An Eco–Friendly Method for the Sanitization of the Dairy Supply Chain
The dairy field has considerable economic relevance in the agri-food system, but also has the need to develop new ‘green’ supply chain actions to ensure that sustainable products are in line with consumer requirements. In recent years, the dairy farming industry has generally improved in terms of equipment and product performance, but innovation must be linked to traditional product specifications. During cheese ripening, the storage areas and the direct contact of the cheese with the wood must be carefully managed because the proliferation of contaminating microorganisms, parasites, and insects increases significantly and product quality quickly declines, notably from a sensory level. The use of ozone (as gas or as ozonated water) can be effective for sanitizing air, water, and surfaces in contact with food, and its use can also be extended to the treatment of waste and process water. Ozone is easily generated and is eco-sustainable as it tends to disappear in a short time, leaving no residues of ozone. However, its oxidation potential can lead to the peroxidation of cheese polyunsaturated fatty acids. In this review we intend to investigate the use of ozone in the dairy sector, selecting the studies that have been most relevant over the last years.
Philippines – Dozens downed by food poisoning in Sulu
ZAMBOANGA CITY: Dozens of villagers in the southern province of Sulu were rushed to hospital on suspicion of food poisoning after they consumed snacks given by soldiers, radio reports said.
Radio Pilipinas Jolo said at least 63 people, including 47 children, complained of dizziness and stomach ache after eating “ginataan” handed out by soldiers on Friday in the village of Tagbak in Indanan town.
The radio station also quoted Dr. Alhazer Siraji Ismael, of the Sulu Sanitarium Hospital, that most of the patients suffered from severe dehydration.
Ismael said six of the patients had to be monitored but the others were in stable condition.
Among those in the hospital were Girls Scout Executive Helen Hajan, and her daughter. The military did not issue any statement on the incident.
Posted in Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Poisoning, Foodborne Illness, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk
Switzerland – Swiss checks find microbially contaminated fruit salads
Listeria and coagulase-positive Staphylococci have been found during inspections of fruit salads in Switzerland, according to the Swiss Association of Cantonal Chemists (ACCS).
Cantonal chemists analyzed the microbiological quality of fruit salads by taking 205 samples.
During the control, organized from May to September 2022, the samples of fruit salads were taken throughout Switzerland and Liechtenstein to check the microbial quality and for the presence of bacteria that could pose a health risk.
Food control is done by cantons, which carry out inspections and laboratory analyses with the help of cantonal chemists. Switzerland is made up of 26 regions called cantons.
Fruit salads can represent a health risk, particularly if storage temperatures and times are not adequate and products are intended for vulnerable people in retirement homes, nursing homes, or hospitals.
Targeted sampling looked at types of fruit more likely to be contaminated by bacteria such as low-acid fruits like melon or watermelon and followed criteria set in food legislation.
Posted in bacterial contamination, cross contamination, food contamination, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Listeria, Listeria monocytogenes, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Staphylococcal Toxin, Staphylococcus aureus
Research – Food safety considerations in the production of traditional fermented products: Japanese rice koji and miso
Abstract
While established in Asia, rice koji and miso are fermented foods that are becoming more popular in western countries. They have been shown to contain a variety of microorganisms, consisting of bacteria, yeasts, and fungal species. Many contemporary miso varieties are not pasteurized as consumers are looking for more natural products, and/or have the desire to consume fermented foods containing live microorganisms. While correctly prepared fermented foods are rarely associated with food safety outbreaks, incidences have been recorded. On these occasions, pathogenic, or spoilage microorganisms were introduced into the products from external sources such as the raw material or the processing environment. Consequently, hygiene and fermentation conditions need to be carefully monitored to ensure food safety. Furthermore, many of the production steps during koji and miso manufacture do not fit into contemporary food safety guidelines for foods. Although pH is a required food safety hurdle for fermented foods, this does not apply to nonacidic foods such as koji or miso. This review focuses on control of microbial pathogens and discusses the processes of miso fermentation, and how fermentation of rice koji and miso fits with current food safety hurdles in western countries.
Argentina – Tainted meat linked to two deaths in Argentina
Argentinian officials have confirmed leptospirosis and Salmonella in an outbreak that has killed two people.
Authorities in Buenos Aires reported that four adults were hospitalized in the city of Berazategui in early 2023. Salmonella and Shigella were detected in the fatal cases and in one patient it was Salmonella Typhimurium. Leptospirosis was also confirmed in two cases under investigation.
The deaths were in two men aged 48 and 36. A 40-year-old patient was discharged from the hospital at the end of January while a 47-year-old is recovering.
Initial investigations found all patients had a recent history of eating meat and meat products such as offal. Authorities said this led them to suspect a common food as the source of the outbreak.
In two cases, there was a link to a butcher shop. However, testing of seized products was negative for Salmonella, E. coli, and Shigella. For another patient, one food sample was positive for Salmonella.
Samples were sent for analysis to the National Food Safety and Quality Service (SENASA) and National Food Institute (INAL). Authorities in Berazategui also closed one butcher shop because of hygiene problems.
Bacteria that cause leptospirosis are spread through the urine of infected animals, which can get into water or soil and survive for weeks to months. People can get infected by consuming contaminated food or water.
Finland – Several cases of food poisoning caused by oysters confirmed in Helsinki

The Environment Services of the City of Helsinki is investigating a number of suspected cases of food poisoning that are believed to be related to dining at several different restaurants and a pop-up event since the beginning of February. Currently, there are about 20 known cases of illness.
The individuals who have fallen ill all consumed oysters, and environmental services have taken food samples from the restaurants as well as patient samples from the city’s epidemiological action. Norovirus has been found in the samples.
Those who have fallen ill are encouraged to contact the food safety unit primarily through an electronic food poisoning form at https://ilppa.fi. Contact can also be made by phone on weekdays from 8 am to 4 pm at +358 9 310 31527.
Norovirus is the most common cause of sudden intestinal infections in Finland.
Posted in food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Norovirus, Norovirus Oysters, outbreak
Quebec – Notice not to consume duck and pork rillettes prepared and sold by the Chez Valère cafeteria at the Université de Montréal – Food Safety
(MAPAQ), in collaboration with the Food Inspection Division of the City of Montreal and the Chez Valère cafeteria of the University of Montreal , located at 3200, rue Jean-Brillant, in Montreal, warns the public not to consume the product indicated in the table below, because it has not been prepared and packaged in such a way as to ensure its safety.
|
Product name |
Format |
Affected batch |
|
“Duck and pork rillettes” |
UNITARY |
Units sold until February 20, 2023 |
The product that is the subject of this warning was offered for sale until February 20, 2023, at the establishment designated above as well as at the Bistro sur la montagne convenience store, located at 2350 , boulevard Édouard-Montpetit, in Montreal. The product was packaged in a glass jar and sold at room temperature.
The operator is voluntarily recalling the product in question. It has agreed with MAPAQ and the Food Inspection Division of the City of Montreal to distribute this warning as a precautionary measure. In addition, people who have this product in their possession are advised not to consume it. They must return it to the establishment where they bought it or throw it away. Even if the affected product shows no signs of tampering or suspicious odors, its consumption may represent a health risk.
It should be noted that no case of illness associated with the consumption of this food has been reported to MAPAQ to date.
Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Quality, Food Safety, Food Safety Alert, Food Safety Management, Food Spoilage, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk
UK – APHA part of 5 UK E. coli outbreaks in 2022
The Animal and Plant Health Agency (APHA) was involved in another two E. coli outbreaks in the United Kingdom in the final quarter of 2022.
In the first, APHA helped Public Health Wales investigate two human cases of Shiga toxin-producing E. coli (STEC) O145 linked to a private collection of animals on a smallholding. Multiple species were onsite including cattle, goats, deer, and pigs. Some food consumed by people was grown in the garden where cattle manure was used. Thirty environmental fecal samples were collected but a match to the outbreak strain was not found.
In the second, APHA visited an open farm at the request of an incident management team following an outbreak of E. coli O157 in people. The outbreak strain was detected in one environmental sample from a pig enclosure. The incident is ongoing so it is unclear how many people are sick. The advice was provided to reduce the risk to the public by making improvements to the supervision of animal contact, enhancing handwashing facilities, and improving some animal exhibits.
In all of 2022, APHA was part of five E. coli investigations. The agency helped the UK Health Security Agency (UKHSA) investigate E. coli O103, O145, and O26 outbreaks between July and September.
The E. coli O26 outbreak also involved cryptosporidium. There were 11 cases of cryptosporidium and two people had confirmed E. coli O26. Cryptosporidium patients had visited an open farm attraction during the incubation period of illness. The E. coli patients had links to the same premises.
The E. coli O103 outbreak with 11 cases was associated with soft, raw milk cheese from a dairy farm in the East of England. Pasteurization was put in place for the production of the soft cheese, HACCP processes were reviewed, and enhanced control measures were taken.
The E. coli O145 outbreak with 10 patients was traced to the consumption of milk products from a dairy farm in North West England, with illness onset from mid-July. Investigations identified an issue with pasteurization and problems with the cleaning and storage of milk crates.
Posted in APHA, Cryptosporidiosis, Cryptosporidium, E.coli, E.coli O103, E.coli O145, E.coli O157, E.coli O157:H7, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, HACCP, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, O103, STEC, STEC E.coli, UKHSA
Research – A Systematic Quantitative Determination of the Antimicrobial Efficacy of Grape Seed Extract against Foodborne Bacterial Pathogens
Abstract
Concerns regarding the role of antimicrobial resistance (AMR) in disease outbreaks are growing due to the excessive use of antibiotics. Moreover, consumers are demanding food products that are minimally processed and produced in a sustainable way, without the use of chemical preservatives or antibiotics. Grape seed extract (GSE) is isolated from wine industry waste and is an interesting source of natural antimicrobials, especially when aiming to increase sustainable processing. The aim of this study was to obtain a systematic understanding of the microbial inactivation efficacy/potential of GSE against Listeria monocytogenes (Gram-positive), Escherichia coli and Salmonella Typhimurium (Gram-negative) in an in vitro model system. More specifically, for L. monocytogenes, the effects of the initial inoculum concentration, bacterial growth phase and absence of the environmental stress response regulon (SigB) on the GSE microbial inactivation potential were investigated. In general, GSE was found to be highly effective at inactivating L. monocytogenes, with higher inactivation achieved for higher GSE concentrations and lower initial inoculum levels. Generally, stationary phase cells were more resistant/tolerant to GSE as compared to exponential phase cells (for the same inoculum level). Additionally, SigB appears to play an important role in the resistance of L. monocytogenes to GSE. The Gram-negative bacteria under study (E. coli and S. Typhimurium) were less susceptible to GSE as compared to L. monocytogenes. Our findings provide a quantitative and mechanistic understanding of the impact of GSE on the microbial dynamics of foodborne pathogens, assisting in the more systematic design of natural antimicrobial-based strategies for sustainable food safety.
Posted in Antimicrobials, Decontamination Microbial, escherichia coli, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Safety, Food Safety Management, Food Safety Regulations, food safety training, Listeria, Listeria monocytogenes, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Salmonella
Research – Microbial Biocontrol in the Agri-Food Industry
Abstract
In recent years there has been a growing interest in the use of natural antimicrobial compounds to limit or avoid the use of chemical antimicrobials. Natural antimicrobial compounds can come from plants (essential oils) or from microorganisms (bacteriocins, mycocines, and active peptides). Despite a wide range of possible applications, their exploitation at the industrial level is still limited and needs to be investigated. The actual and possible applications of natural antimicrobial compounds in agri-food are a growing research field. In addition to the use of antimicrobial compounds, microorganisms themselves can be used in the control of spoilage microorganisms along the entire production chain of the agri-food industry. Likewise, the papers collected in this Special Issue indicate the fast development of novelties in this research field.
