Category Archives: Food Microbiology Research

Research – Vapor Phase Hydroxyl- or Chlorine-radical Treatment for Inactivating Listeria monocytogenes on Mushrooms (Agaricus bisporus) Without Negatively Affecting Quality or Shelf-life

Journal of Food Protection

Processes based on generating vapor phase hydroxyl-radicals or chlorine-radicals were developed for inactivating Listeria monocytogenes on mushrooms without negatively affecting quality. Antimicrobial radicals were generated from the UV-C degradation of hydrogen peroxide or hypochlorite and ozone gas. Response Surface Modelling (RMS) was used to identify the interaction between the operating parameters for the hydroxyl-radical process; UV-C 254nm intensity, hydrogen peroxide concentration and ozone delivered. There was an inverse relationship between hydrogen peroxide concentration and UV-C intensity in terms of the log reduction of L. monocytogenes . The independent parameters for the chlorine-radical process were hypochlorite concentration, pH, and UV-C intensity. From predictive models, the optimal hydroxyl-radical treatment was found to be 5% v/v H 2 O 2 , 2.86 mW/cm 2 UV-C intensity (total UV-C dose 144 mJ/cm 2 ) and 16.5 mg ozone. The chlorine-radical optimal process parameters were 10 ppm hypochlorite (pH 3.0), ozone 11.0 mg and 4.60 mW/cm 2 UV-C intensity. When inoculated mushrooms were treated with the optimal hydroxyl-radical and chlorine-radical process the log CFU reduction of L. monocytogenes was found to be 2.42±0.42 and 2.61±0.30 log CFU respectively without any negative effects on mushroom quality (weight loss and Browning Index during 14 days storage at 4°C). The levels of L. monocytogenes inactivation were significantly greater compared to when the individual elements of the radical processes were applied and control using a 90 s dip in 1% v/v hydrogen peroxide. The study has demonstrated that both hydroxyl-radical and chlorine-radical vapor-phase treatments are both equally effective at inactivating L. monocytogenes on mushrooms and can be considered as a preventative control step.

Research – Shiga Toxin-Producing Escherichia coli (STEC) and STEC-Associated Virulence Genes in Raw Ground Pork in Canada

Journal of Food Protection

Shiga toxin-producing Escherichia coli (STEC) O157:H7/NM and some non-O157 STEC are foodborne pathogens. In response to pork-associated O157 STEC outbreaks in Canada, we investigated the occurrence of STEC in Canadian retail raw ground pork during the period of November 1, 2014 and March 31, 2016. Isolated STEC were characterized to determine the Shiga-toxin gene ( stx ) subtype and the presence of virulence genes encoding intimin ( eae ), and enterohemorrhagic E. coli hemolysin (hlyA) . O157 STEC and non-O157 STEC were isolated from 0.11% (1/879) and 2.24% (13/580) of the pork samples. STEC virulence gene profiles containing both eae and hlyA were found only in the O157 STEC ( stx 2a , eae , hlyA ) isolate. The eae gene was absent from all non-O157 STEC isolates. Of the 13 non-O157 STEC isolates, two virulence genes of stx 1a and hlyA were found in four (30.8%) O91:H14 STEC isolates, while one virulence gene of stx 2e, stx 1a , and stx 2a was identified in five (38.5%), two (15.4%) and one (7.7%) STEC isolates respectively of various serotypes. The remaining non-O157 STEC isolate carried stx 2 , but the subtype is unknown as this isolate could not be recovered for sequencing. O91:H14 STEC ( stx 1a, hlyA ) was previously reported in association with diarrhea illnesses, while the other non-O157 STEC isolates identified in this study are not known to be associated with severe human illnesses. Virulence gene profiles identified in this study indicate that the occurrence of non-O157 STEC capable of causing severe human illness is rare in Canadian retail pork. However, O157 STEC in ground pork can occasionally occur, therefore education regarding the potential risks associated with STEC contamination of pork would be beneficial for the public and those in the food industry in order to help reduce foodborne illnesses.

Research -Evidence of on-going transmission of Shiga toxin-producing Escherichia coli O157:H7 following a foodborne outbreak

Cambridge Org

In August 2019, public health surveillance systems in Scotland and England identified seven, geographically dispersed cases infected with the same strain (defined as isolates that fell within the same five single nucleotide polymorphism single linage cluster) of Shiga toxin-producing Escherichia coli O157:H7. Epidemiological analysis of enhanced surveillance questionnaire data identified handling raw beef and shopping from the same national retailer (retailer A) as the common exposure. Concurrently, a microbiological survey of minced beef at retail identified the same strain in a sample of minced beef sold by retailer A, providing microbiological evidence of the link. Between September and November 2019, a further four primary and two secondary cases infected with the same strain were identified; two cases developed haemolytic uraemic syndrome. None of the four primary cases reported consumption of beef from retailer A and the transmission route of these subsequent cases was not identified, although all four primary cases visited the same petting farm. Generally, outbreaks of STEC O157:H7 in the UK appear to be distinct, short-lived events; however, on-going transmission linked to contaminated food, animals or environmental exposures and person-to-person contact do occur. Although outbreaks of STEC caused by contaminated fresh produce are increasingly common, undercooked meat products remain a risk of infection.

Research – Use of Oxidative Stress Responses to Determine the Efficacy of Inactivation Treatments on Cryptosporidium Oocysts

MDPI

crypto

Cryptosporidium oocysts are known for being very robust, and their prolonged survival in the environment has resulted in outbreaks of cryptosporidiosis associated with the consumption of contaminated water or food. Although inactivation methods used for drinking water treatment, such as UV irradiation, can inactivate Cryptosporidium oocysts, they are not necessarily suitable for use with other environmental matrices, such as food. In order to identify alternative ways to inactivate Cryptosporidium oocysts, improved methods for viability assessment are needed. Here we describe a proof of concept for a novel approach for determining how effective inactivation treatments are at killing pathogens, such as the parasite Cryptosporidium. RNA sequencing was used to identify potential up-regulated target genes induced by oxidative stress, and a reverse transcription quantitative PCR (RT-qPCR) protocol was developed to assess their up-regulation following exposure to different induction treatments. Accordingly, RT-qPCR protocols targeting thioredoxin and Cryptosporidium oocyst wall protein 7 (COWP7) genes were evaluated on mixtures of viable and inactivated oocysts, and on oocysts subjected to various potential inactivation treatments such as freezing and chlorination. The results from the present proof-of-concept experiments indicate that this could be a useful tool in efforts towards assessing potential technologies for inactivating Cryptosporidium in different environmental matrices. Furthermore, this approach could also be used for similar investigations with other pathogens. View Full-Text

Research – The serious and long-term effects of foodborne illness

MSU

Campylobacter kswfoodworld

Many people think if they eat something “bad” they might spend some uncomfortable time in the restroom then go on with their lives in a day or two. Actually, foodborne illness can lead to chronic disease, permanent disability and even death. Here are some of the lesser-known facts about the long term effects of food borne pathogens.

Reactive arthritis, a type of joint inflammation that affects the knees, ankles or feet can develop after being exposed to Campylobacter jejuni  or Salmonella. Reactive arthritis usually lasts fewer than 6 months, but this condition may recur or become chronic arthritis. Campylobacter is found in raw and undercooked poultry, unpasteurized milk and contaminated water. Salmonella is also found in eggs, undercooked meat and poultry, and can be transferred to other foods through feces. Campylobacter infection afflicts millions of Americans annually and hospitalizes more than 10,000. Campylobacter is associated with Guillain-Barré syndrome (GBS). GBS patients can become permanently disabled and paralyzed; many require hospital care. Campylobacter also can trigger arthritis, heart infections and blood infections.

Foodborne illnesses caused by bacteria also increase the risk of developing irritable bowel syndrome (IBS). IBS is a disorder of unknown cause that is associated with abdominal pain, bloating, and diarrhea or constipation or both. There is no cure for IBS.

Children are the most likely to be infected and have the most serious complications from E. coli O157:H7. They can develop hemolytic uremic syndrome (HUS), HUS can lead to death, or in some cases t long-term or permanent health problems, including end-stage kidney disease, neurological complications, and insulin-dependent diabetes. Adults who had recovered from E. coli O157:H7 infections had increased risks of high blood pressure, kidney problems and cardiovascular disease. E. Coli is found in undercooked ground beef, unpasteurized (raw) milk and juice, soft cheeses made from raw milk, and raw fruits and vegetables (such as sprouts). It can also be found in contaminated water and feces of infected people.

Listeria monocytogenes infects thousands of Americans every year and has been associated with infections of the brain and spinal cord, resulting in serious neurological dysfunctions or death. Most reported cases occur in children under the age of 4, and about 1 in 5 people afflicted die as a result of the infection. In pregnant women, listeriosis can cause miscarriage, premature birth or stillbirth.

Listeriosis survivors often are left with serious neurological dysfunctions, including seizures, paralysis and impaired ability to see, hear, swallow or speak. Severe cases often result in partial to total impairment and can require life-long residential care with no possibility of work. Listeria is found in ready-to-eat deli meats and hot dogs, refrigerated pâtés or meat spreads, unpasteurized (raw) milk and dairy products, soft cheese made with unpasteurized milk, such as queso fresco, feta, brie, camembert, refrigerated smoked seafood and raw sprouts.

The long-term effects of foodborne disease are still not fully understood. New findings unfold as research is performed, but until then, taking foodborne illness seriously can go a long way in protecting health. This is another instance where prevention truly is the best medicine.

Finland – Salmonella outbreak affects 450 in Jyväskylä, Linked to imported lettuce

Outbreak News Today

In a follow-up on the salmonella outbreak in the Jyväskylä region of Finland, the City of Jyväskylä said imported lettuce served in day care centres has been confirmed as the source of an outbreak of salmonella bacterial infections that affected about 450 children and day care centre staff.

The salad contained domestic cucumber, peas and foreign iceberg lettuce. Domestically-produced cucumbers and peas were also suspected to be the cause of the outbreak, but the city said tests had revealed no connection between these products and the salmonella infections.

The most likely source of Salmonella is iceberg lettuce, which came to Finland from Germany via Sweden.

Research – FAO’s work on food safety and quality

FAO

With an estimated 600 million cases of foodborne illnesses annually, unsafe food is a threat to human health and economies globally. Therefore, ensuring food safety is a public health priority and an essential step to achieving food security. Effective food safety and quality control systems are key not only to safeguarding the health and well-being of people, but also to fostering economic development and improving livelihoods by promoting access to domestic, regional and international markets.

The Food Safety and Quality Unit supports the strengthening of systems of food safety and quality control at national, regional and international levels. This involves:

  • Strengthening national food control regulatory capacities and global trade facilitation by providing leadership in supporting countries in the assessment and progressive development of food control systems, including food safety policy and food control regulatory frameworks;
  • Supporting development of institutional and individual capacities for food control and food safety management, including the management of food safety emergencies;
  • Supporting science-based food safety governance and decisions by providing sound scientific advice (through the JECFA and JEMRA expert bodies) to underpin food safety standards at national, regional and international levels;
  • Enhancing food safety management along food chains to prevent diseases and trade disruptions by supporting developing countries to apply risk-based food safety management along food chains that are appropriate for national and local production systems and in compliance with Codex texts;
  • Providing food safety platforms, databases and mechanisms which support networking, dialogue and global access to information and facilitating effective communication internationally on key food safety issues;
  • Developing food safety intelligence and foresight by becoming a major actor in the collection, analysis and communication of food chain intelligence; and
  • Evaluating new technologies to improve food safety and protect public health.

FAO is a recognized leader in the development of global food safety initiatives and translating these into country level action. The Food Safety and Quality Programme supports an integrated and multidisciplinary approach to food safety management and holistic and feasible “food chain” solutions to specific food safety problems as laid out in FAO’s Strategy for Improving Food Safety Globally. The foundations for this approach are based on science.

FAO’s Food Safety and Quality Unit often works in partnership with national and international bodies and organizations where such partnerships are mutually beneficial and where there is a compatibility of mandate and guiding principles.

Ireland – A Foodborne Outbreak of Cryptosporidiosis Likely Linked to Salad Leaves

IMJ

Click to access A-Foodborne-Outbreak-of-Cryptosporidiosis-Likely-Linked-to-Salad-Leaves.pdf

A Silver Lining of the Pandemic: Whole-Genome Sequencing and Food Safety

FAO

Zooming out and taking a broader perspective, WGS benefits are particularly visible in microbiology: WGS provides rapid identification and characterization of microorganisms with a level of precision that no tool has ever reached before. Through WGS, specific novel antibiotic targets (resistance genotypes) are being identified in the area of antimicrobial resistance (AMR)studies. It is a significant stepping-stone because more focused surveillance and more targeted diagnostics and drug development are made possible by identifying them and correlating them to the observed phenotypes. The level of detail provided by WGS makes it possible to refine case definitions precisely, which in turn allows for faster investigation of outbreak clusters, thus preventing additional cases of diseases sooner. For example, in 2014, a multinational Salmonella outbreak investigation was conducted in Europe where WGS was used to identify the root cause, and the data collected pointed to some egg farms in a specific location. While WGS was essential in the investigation, it was the international data-sharing efforts that made the investigation successful and conclusive. Another example is the outbreak investigation of Listeria linked to enoki mushrooms in Canada where the specific sequence of the Listeria strain was internationally shared, resulting in successfully tracing the multinational food safety outbreak and the rapid recall of the affected products.

Research – A Review of Significant European Foodborne Outbreaks in the Last Decade

Journal of Food Protection

Vegetable Bacteria Danger

Foodborne diseases remain a global public health challenge worldwide. The European surveillance system on multistate foodborne outbreaks integrates elements from public and animal health, and the food chain for the early detection, assessment, and control. This review aims to describe the significant outbreaks that occurred in Europe in the last decade. Their significance and relevance in public health laid in the changes, improvements, and novelties that derived and that pushed towards the building of a safer food system in the European Union, certainly driven by the One Health approach. In 2011, a point source monoclonal outbreak of infections caused by Escherichia coli serotype O104:H4 contaminating sprouted seeds recorded hundreds of cases of haemolytic-uremic syndrome and several fatalities. In 2015, a prolonged outbreak of Listeria monocytogenes infections caused by the contamination of frozen corn affected Europe with 47 cases and nine deaths. In 2016, a persistent polyclonal outbreak of Salmonella Enteritidis was linked to the consumption of eggs and was associated with hundreds of cases. These outbreaks commonly highlighted the importance of sharing data (e.g. sequencing and tracing data) with rapidity and the need for harmonizing bioinformatics outputs and computational approaches to facilitate foodborne detection and investigation. Also, they led to the setting of the legal framework for the development of a European collaboration platform to share whole genome sequences data. These outbreaks enabled the enforcement of the existing hygiene and food safety provisions and led the development of new hygiene guidelines and best practises. This paper also briefly touches upon the new trends in information technologies that are being explored in the field of food traceability and safety. Their application aims to enhance the traceability of food throughout the supply chain to redirect the conventional tracing system towards a digitized supply chain.