Category Archives: Bacillus

RASFF Alert- Bacillus cereus – Dried Mushrooms

Posted on January 5, 2024 by | Leave a comment

RASFF

Bacillus cereus in dried mushrooms from China, via Germany in Austria

Leave a comment

Posted in Bacillus, Bacillus cereus, food contamination, food handler, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Quality, food recall, Food Safety, Food Safety Alert, Food Safety Management, Food Safety Regulations, Food Testing, RASFF

RASFF Alert- Bacillus cereus – Calzini Chicken

Posted on December 15, 2023 by | Leave a comment

RASFF

Bacillus cereus (6.0E5 CFU/g) in Calzini Chicken from the Netherlands in Switzerland, lativa and italy

Leave a comment

Posted in Bacillus, Bacillus cereus, food contamination, food handler, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Poisoning, Food Quality, food recall, Food Safety, Food Safety Alert, Food Safety Management, Food Safety Regulations, Food Testing, Food Toxin, RASFF

China – Rice noodles behind Bacillus cereus outbreak in China

Posted on November 20, 2023 by | Leave a comment

Food Safety News

A large outbreak with almost 200 cases in China was caused by poor hygiene at the producer and inadequate food storage at schools, according to a recent study.

An outbreak of acute gastrointestinal illness occurred at two middle schools in a rural region of Chongqing in 2021. The source was rice noodles contaminated with Bacillus cereus.

In May 2021, an outbreak was reported in the two schools. More than 100 students from the schools had symptoms of vomiting and nausea, prompting the Chongqing Center for Disease Control and Prevention and the local CDC to investigate and implement control measures.

Leave a comment

Posted in Bacillus, Bacillus cereus, food bourne outbreak, Food Illness, Food Microbiology Research, Food Poisoning, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, outbreak, Poisoning, Research

Research-Contamination of Plant Foods with Bacillus cereus in a Province and Analysis of Its Traceability

Posted on November 19, 2023 by | Leave a comment

MDPI

Abstract

Bacillus cereus is an important zoonotic foodborne conditional pathogen. It is found in vegetables, dairy products, rice, and other foods, thereby greatly endangering human health. Investigations on B. cereus contamination in China primarily focus on raw milk, dairy products, meat, and others, and limited research has been conducted on plant-based foodstuffs. The rapid development of sequencing technology and the application of bioinformatics-related techniques means that analysis based on whole-genome sequencing has become an important tool for the molecular-epidemiology investigation of B. cereus. In this study, we investigated the contamination of B. cereus in six types of commercially available plant foods from eight regions of a province. The molecular epidemiology of the isolated B. cereus was analyzed by whole-genome sequencing. We aimed to provide fundamental data for the surveillance and epidemiology analysis of B. cereus in food products in China. The rapid traceability system of B. cereus established in this study can provide a basis for rapid molecular epidemiology analysis of B. cereus, as well as for the prevention and surveillance of B. cereus. Moreover, it can also be expanded to monitoring and rapid tracing of more foodborne pathogens.

Leave a comment

Posted in Bacillus, Bacillus cereus, Food Microbiology Research, Research, Zoonosis

RASFF Alert- Toxin Producing Bacillus cereus –

Posted on November 10, 2023 by | Leave a comment

RASFF

Toxin-producing Bacillus cereus in sprouted organic broccoli seeds from Germany, packaged in Romania in  Austria, Bulgaria, Germany, Netherlands, Portugal, Romania, Switzerland.

Leave a comment

Posted in Bacillus, Bacillus cereus, Bacterial Toxin, food contamination, food handler, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Quality, food recall, Food Safety, Food Safety Alert, Food Safety Management, Food Safety Regulations, Food Testing, Food Toxin

Germany – Govinda Germinated broccoli seeds – Bacillus cereus

Posted on November 9, 2023 by | Leave a comment

Lebensmittelwarnung

Alert type: Groceries
Date of first publication:

November 8, 2023

Product name: govinda Germinated broccoli seeds
Product images:

Label front broccoli seeds.JPG

Govinda

Manufacturer (distributor):

Govinda Natur GmbH, Joseph-Monier-Str. 12, 67433 Neustadt an der Weinstrasse

Reason for warning:

Bacillus cereus

Packaging Unit: 125g
Durability: Best before: September 15, 2025
Lot identification: 2803/3

Leave a comment

Posted in Bacillus, Bacillus cereus, food recall, Lebensmittelwarnung, product recall, Recall

Hong Kong – Kindergarten teacher and 10 kids affected in suspected food poisoning cluster

Posted on November 6, 2023 by | Leave a comment

The Standard HK

The Centre for Health Protection is now investigating a suspected food poisoning cluster affecting 10 children and a female teacher who have consumed steamed rice with pumpkin and diced pork.

The cluster involved eight boys and two girls, aged 3 to 5, and a female teacher, who developed abdominal pain and diarrhea about 5.5 to 15 hours after having lunch at a kindergarten in Eastern District on Wednesday.

One of them sought medical advice and none required hospitalization. All the kids and the teacher are in stable conditions.

Initial investigation of the CHP revealed that the affected persons had consumed common food and the food concerned was steamed rice with pumpkin and diced pork.

The poisoning might have been caused by Bacillus cereus or Clostridium perfringens; the CHP noted.

“The personnel from the Centre for Food Safety of the Food and Environmental Hygiene Department have conducted an investigation at the concerned premises. The CHP’s investigation is ongoing,” it said.

Leave a comment

Posted in Bacillus, Bacillus cereus, Food Poisoning, food recall, Poisoning, product recall, Recall, Staphylococcal Toxin, Staphylococcus aureus

RASFF Alert – Spore Forming Bacteria – Duck Confit

Posted on November 3, 2023 by | Leave a comment

RASFF

Possible presence of spore-forming bacteria (possibly bacillus spp.) in Confit de Canad from Bulgaria in Denmark

Leave a comment

Posted in Bacillus, Bacterial Spores, food contamination, food handler, Food Hazard, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, Food Pathogen, Food Quality, food recall, Food Safety, Food Safety Alert, Food Safety Management, Food Safety Regulations, Food Testing, RASFF, Spores

Research – Biocide Tolerance and Impact of Sanitizer Concentrations on the Antibiotic Resistance of Bacteria Originating from Cheese

Posted on November 1, 2023 by | Leave a comment

MDPI

Abstract

In this study, we determined and identified the bacterial diversity of different types of artisanal and industrially produced cheese. The antibiotic (erythromycin, chloramphenicol, kanamycin, ampicillin, clindamycin, streptomycin, tetracycline, and gentamicin) and biocide (peracetic acid, sodium hypochlorite, and benzalkonium chloride) resistance of clinically relevant bacteria was determined as follows: Staphylococcus aureus, Macrococcus caseolyticus, Bacillus sp., Kocuria varians, Escherichia coli, Enterococcus faecalis, Citrobacter freundii, Citrobacter pasteurii, Klebsiella oxytoca, Klebsiella michiganensis, Enterobacter sp., Enterobacter cloacae, Enterobacter sichuanensis, Raoultella ornithinolytica, Shigella flexneri, and Salmonella enterica. Also, the effect of the sub-inhibitory concentration of three biocides on antibiotic resistance was determined. The microbiota of evaluated dairy products comprise diverse and heterogeneous groups of bacteria with respect to antibiotic and disinfectant tolerance. The results indicated that resistance was common in the case of ampicillin, chloramphenicol, erythromycin, and streptomycin. Bacillus sp. SCSSZT2/3, Enterococcus faecalis SRGT/1, E. coli SAT/1, Raoultella ornithinolytica MTT/5, and S. aureus SIJ/2 showed resistance to most antibiotics. The tested bacteria showed sensitivity to peracetic acid and a different level of tolerance to benzalkonium chloride and sodium hypochlorite. The inhibition zone diameter of antibiotics against Enterococcus faecalis SZT/2, S. aureus JS11, E. coli CSKO2, and Kocuria varians GRT/10 was affected only by the sub-inhibitory concentration of peracetic acid.

Leave a comment

Posted in Bacillus, Biocide, Enterobacteriaceae, Food Microbiology Research, Research, Shigella, Shigella flexneri, Staphylococcus aureus

Research – Role of Bacillus cereus biofilm formation behavior in virulence and pathogenic characteristics

Posted on October 29, 2023 by | Leave a comment

Wiley Online

Abstract

This study aimed to examine the potential link between Bacillus cereus biofilm formation, virulence, and pathogenicity. The biofilm formation abilities of nine B. cereus strains isolated from food and two reference strains (ATCC 10876 and ATCC 25621) were measured using a crystal violet assay. Among the tested strains, three strains (GIHE 617-5, GIHE 86-09, and GIHE 728-17) and both reference strains were capable of biofilm formation. A positive correlation was obtained for higher cell surface hydrophobicity and increased biofilm formation. In contrast, HPLC analysis for elevated autoinducer-2 (Al-2) production revealed a negative impact on biofilm formation. PCR data indicated that all tested strains were capable of producing common B. cereus enterotoxins, including Hbl—A, C, and D, CytK, Nhe—B and C, EntFM, and BceT, but were negative for production of the emetic toxin cereulide and the pore-forming toxin Hly II. Meanwhile, RT-PCR data revealed a close correlation between high biofilm formation and the upregulation of several tested virulence genes for selected strains. However, elevated upregulation of virulence genes was not consistent in all of the higher biofilm-forming strains. Cytotoxicity analysis revealed higher virulence characteristics compared to those of low biofilm-forming strains.

Leave a comment

Posted in Bacillus, Bacillus cereus, Biofilm, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Research, Technology