Category Archives: Microbiology

Research – Joint FAO/WHO Expert Meeting on Microbiological Risk Assessment (JEMRA) on Shiga toxin-producing Escherichia coli (STEC) associated with meat and dairy products

FAO

Shiga toxin-producing Escherichia coli (STEC) are an important cause of food-borne disease. Infections can result in a wide range of disease symptoms from mild intestinal discomfort and hemorrhagic diarrhea to severe conditions including haemolytic uremic syndrome (HUS), end-stage renal disease and death. In its report on the global burden of food-borne disease, WHO estimated that in 2010 food-borne STEC caused more than 1.2 million illnesses, 128 deaths, and nearly 13 000 Disability Adjusted Life Years (DALYs) (WHO, 2015). The Codex Committee on Food Hygiene (CCFH) has highlighted the importance of STEC in foods since its 32nd Session in 1999, when it prioritized their presence in beef and sprouts as significant public health problems in Member countries (FAO and WHO, 2000). Following a request from the 47th Session in November 2015 (FAO and WHO, 2016), the FAO and WHO published the report Shiga toxin-producing Escherichia coli (STEC) and food: attribution, characterization and monitoring in 2018 (FAO and WHO, 2018). As part of the 50th session of CCFH in November 2018, the FAO/WHO further updated the committee with additional information on STEC that was subsequently published in the report Attributing illness caused by Shiga toxin-producing Escherichia coli (STEC) to specific foods (FAO and WHO, 2019a). The Codex Alimentarius Commission (CAC) at the 42nd Session, July 2019, approved new work on the development of guidelines for the control of STEC in beef, raw milk and cheese produced from raw milk, leafy greens and sprouts (FAO and WHO, 2019b). To support this work, the Joint FAO/WHO Expert Meeting on Shiga toxin-producing Escherichia coli(STEC) associated with Meat and Dairy Products was convened virtually from 1 to 26 June 2020 to review relevant measures for pre- and post-harvest control of STEC in animals and foods of animal origins. The scientific literature describing physical, chemical and biological control measures (and their combinations) against STEC during primary production, processing and post-processing of raw meat, raw milk and raw milk cheeses was reviewed. The efficacy and utility of reported control measures were scored as high, medium or low, based on expert opinion informed by systematic reviews and/or meta-analyses, when available.

ECDC – Legionnaires’ disease – Annual Epidemiological Report for 2020

ECDC

ECDC’s annual surveillance reports provide a wealth of epidemiological data to support decision-making at the national level. They are mainly intended for public health professionals and policymakers involved in disease prevention and control programmes.

Executive summary

  • Legionnaires’ disease remains an uncommon and mainly sporadic respiratory infection with an overall notification rate of 1.9 cases per 100 000 population for the EU/EEA in 2020.
  • A small decrease in the annual notification rate was observed, down from the 2.2 cases per 100 000 population reported in 2019.
  • Notification rates remained heterogenous across the EU/EEA, varying from fewer than 0.5 cases per 100 000 population to 5.7 cases per 100 000 population, with the highest rate reported by Slovenia.
  • Four countries (France, Germany, Italy and Spain) accounted for 72% of all notified cases.
  • Males aged 65 years and older were most affected (7.1 cases per 100 000 population).
  • The number of reported cases to the travel-associated surveillance scheme decreased by 67% in 2020 compared with 2019.
  • Only 10% of cases were culture confirmed (10%), likely leading to underestimation of disease caused by Legionella species other than Legionella pneumophila.

Research – Study Highlights Importance of Cooking Non-Ready-to-Eat Frozen Vegetables, Fruits and Herbs Prior to Eating

FSAI

The Food Safety Authority of Ireland (FSAI) and safefood today released findings from a comprehensive study which shows a potential low-level risk of illness for consumers who eat non-ready-to-eat* frozen vegetables, fruits or herbs without prior cooking. The microbiological study analysed almost 1,000 samples of frozen vegetables, fruits and herbs for the presence of Listeria monocytogenes,SalmonellaListeria spp. and E. coliListeria monocytogenes was found in 27 of the samples tested (3%), the majority of which were non-ready-to-eat frozen vegetables (21 samples).

The FSAI and safefood stress that whilst the figures were low for the presence of Listeria monocytogenes – it is a potential health risk for people who may eat these non-ready-to-eat frozen foods uncooked frequently, and/or if consumer preparation and handling practices allow Listeria monocytogenes, if present, to grow to levels high enough to cause listeriosis. The consumer research part of the study surveyed 815 people across the island of Ireland and found that 80% said they regularly eat frozen vegetables, 40% eat frozen fruits, and 13% eat frozen herbs. While most (68%) said they would cook the product before eating, others (32%) said they regularly eat one or more types of frozen vegetables, fruits or herbs without cooking them. Strawberries, blueberries, raspberries, and mixed berries were the frozen fruits most likely to be eaten uncooked in a dessert or in a smoothie. A smaller number of consumers said they would regularly eat uncooked non-ready-to-eat frozen vegetables such as sweetcorn, carrots, peas, peppers, and spinach in a salad or as a garnish.

The FSAI and safefood advise that all non-ready-to-eat frozen vegetables, fruits and herbs should be cooked prior to eating and in accordance with the cooking instructions on the label. If these products are eaten uncooked by people who are immunocompromised, pregnant, the elderly or young children, there is risk of serious illness. Symptoms of Listeria monocytogenes infection can include mild flu-like symptoms, or gastrointestinal symptoms such as nausea, vomiting and diarrhoea.

Commenting at the release of the study, Dr Pamela Byrne, Chief Executive, FSAI, emphasised the importance of correct labelling and that caterers and food service businesses must ensure they are following the manufacturers’ instructions when they are preparing food for their customers.

“It is vital that food manufacturers follow best practice guidelines and ensure frozen products that are not ready-to-eat are clearly labelled as such, with clear cooking instructions. They also need to ensure there are no serving suggestions presented on the packaging which could suggest that the products can be eaten thawed without prior cooking – whether they be frozen vegetables, fruit or herbs. Caterers and food service businesses must check the food labels and cook the frozen products, if instructed, so as to ensure that the food they are serving to their customers is safe to eat.”

Commenting, Dr Gary A Kearney, interim Chief Executive safefood said: “We know from social media that there’s a growing trend for people to eat frozen fruit and vegetables raw in things like smoothies and salads. While the risk of contracting a Listeria infection is low, it’s still a risk you can avoid by reading the manufacturer’s instructions and cooking these frozen foods before you eat them. Those most at risk from a Listeria infection include young children, pregnant women and people with an underlying medical condition or weakened immunity, If the product says, ‘cook before eating’, we would remind people to always follow that advice.”

National microbiological survey and consumer habits in relation to frozen vegetables, fruits and herbs.

India – Food poisoning: Salmonella, Shigella found in shawarma samples

New Indian Express

THIRUVANANTHAPURAM: The food samples collected from the shawarma outlet at Cheruvathur had the presence of salmonella and shigella bacteria, Health Minister Veena George has said. The chicken shawarma samples had salmonella and shigella while the pepper powder had salmonella. These samples have been confirmed ‘unsafe’ as per the Food Safety Act. A 16-year-old schoolgirl died and 52 persons fell sick after having shawarma from a snacks bar at Cheruvathur recently.

The special drive of the Food Safety Department saw inspections on 349 premises across the state on Saturday. Action was taken against 32 outlets which did not have licence or registration. Notices were served on 119 institutions. Twenty-two kilograms of stale meat was seized. Thirty-two samples were sent for lab test.

Since May 2, 1,132 raids were conducted across the state. Action was taken against 142 outlets which lacked licence or registration. Notice was served on 466 establishments. One hundred and sixty-two kilograms of stale meat was seized and 125 samples were sent for lab test. A total of 6,035 kg of fish, either adulterated or stale, was destroyed. Four hundred and fifty eight outlets were raided as part of ‘Operation Jaggery’ drive, the minister said.

USA – Over 7,300 Lucky Charms Cereal Illness Complaints On Iwaspoisoned.com, FDA Investigating

forbes

You could say that there have been cereal complaints about Lucky Charms. On May 6, 2022, the website iwaspoisoned.com posted the following message: “Starting in late 2021 Lucky Charms food poisoning reports started to trend on iwaspoisoned.com. Now there are reports of over 7,300 sick.”

Yeah, there probably aren’t too many people saying, “gee, I really hope to trend on iwaspoisoned.com.” This website allows people to submit reports of feeling ill after eating a food item that was served at or sold by a business. According to the website, “This real time information is shared by consumers, food authorities, restaurants, and industry with one aim – to make eating a safer experience.” Their stated goal is to prevent “food poisoning outbreaks, reduces risks, and creates better outcomes for restaurants, shareholders, and the public.” Therefore, it’s probably not the place to complain about your significant other’s cooking, unless your significant other happens to be a restaurant or a food manufacturing company.

USA – Wayne Farms, LLC Recalls Ready-To-Eat Chicken Breast Fillet Products That May Be Undercooked

FSIS USDA

EDITOR’S NOTE:  May 7, 2022: This release is being reissued to include an expansion of the recall to a new product that was distributed to retail locations. Additionally, the recall has been expanded from 30,285 pounds to 585,030 pounds. There are five new production codes (23618, 24357, 24512, 24583, and 24957) and 66 different “use by” dates (ranging from 5-10-22 through 4-29-23). This release has been updated to include the new product, the expanded weight, additional labels, and additional distribution information.

WASHINGTON, April 29, 2022 – Wayne Farms, LLC., a Decatur, Ala. establishment, is recalling approximately 585,030 pounds of a ready-to-eat (RTE) chicken breast fillet product that may be undercooked, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.

Research – Prevalence and antimicrobial resistance of non-typhoidal Salmonella enterica from head meat and trim for ground product at pork processing facilities

Journal of Food Protection

Pork head meat may harbor Salmonella and contaminate other carcass byproducts during harvest and fabrication. A large pork processing plant in the United States was sampled bimonthly for 11 months to determine the concentration, prevalence, seasonality, serotype diversity, and antimicrobial susceptibility of Salmonella enterica isolated from cheek meat and head trim of swine carcasses. Each collection consisted of 25 samples on 2 consecutive days in the morning and afternoon shifts, for a total of 100 cheek meat and 100 head trim samples each month. Tissues were cultured for Salmonella using restrictive media and enrichment techniques, and a subset of isolates was serotyped, analyzed for antimicrobial susceptibility, and genome sequenced. Salmonella post-enrichment prevalence did not differ ( P = 0.20) between cheek meat (63%) and head trim (66%). Post-enrichment prevalence differed ( P < 0.05) by month (January 94%; March 80%; May 54%; July 59%; September 47%; and November 55%), and by processing shift (morning 68%; afternoon 62%). The subset (n = 618) of isolates selected for serotyping yielded 21 distinct serotypes: Typhimurium (49%), Infantis (10%), Heidelberg (8%), I 4,[5],12:i- (8%), and 17 other types (≤ 5%). A total of 407 multidrug-resistant (MDR; resistance to 3 or more antibiotic classes) isolates were identified. There were 120 isolates that exhibited the penta-resistant ACSSuT phenotype. In addition, 113 isolates exhibited decreased susceptibility to ciprofloxacin (DSC; MIC ≥ 0.12 µg/mL). Resistance genes bla CARB , bla SHV , bla TEM , aac(6′)-Ib-cr , qnrB , sul2 , and dfrA were expressed in numerous MDR Salmonella isolates. The data from the present study suggest that pork products from the head, as compared to data reported for carcasses, may have a relatively high prevalence of Salmonella with diverse serotypes and MDR.

Research – Possible explanation for limited reduction of pathogens on radish microgreens after spray application of chlorinated water during growth with disperse contamination spread of abiotic surrogate on leaves

Wiley Online

The purpose of this study was to determine the efficacy of spray application of chlorinated water before harvest on the population of Salmonella enterica Typhimurium and Escherichia coli O157:H7 on radish microgreens. The transfer of abiotic surrogate to radish microgreens was also evaluated to track possible pathogen contamination spread by inoculating seed and growth media. During growth, microgreens inoculated with strains of pathogens were sprayed with chlorinated water at three different concentrations (0.50, 1.00, and 2.00 ± 0.05 ppm free chlorine). Spray application of chlorinated water was performed on microgreens once (day 9), twice (day 8 and 9), three (day 7, 8, and 9), and four times (day 6, 7, 8, and 9). Microgreens were harvested 12 hr after the last application of chlorinated water. Salmonella and E. coli O157:H7 populations were reduced with the increase in chlorine concentration. Chlorinated water reduced Salmonella and E. coli O157:H7 populations up to 1.1 log CFU/g (p < .05) and 0.9 log CFU/g (p > .05), respectively. Images taken under UV illumination provided the visualization of abiotic surrogate spread on cotyledon and upper hypocotyl (all edible parts) of radish microgreen plants regardless of seed or growth media inoculation. Scanning Electron Microscopy showed the presence of abiotic surrogate and generic E. coli on microgreen leaves. Spray application of chlorinated water during microgreen growth may help to reduce microbial load but cannot be used as the only control measure.

Research – Microbiologists get grant to study biofilms guarding foodborne pathogen Listeria

Mirage News

Microbiologists in Penn State’s College of Agricultural Sciences have received a $605,000 grant from the U.S. Department of Agriculture to study how microbial biofilms protect Listeria monocytogenes, the bacterium that causes the deadly foodborne illness listeriosis.

Jasna Kovac, Lester Earl and Veronica Casida Career Development Professor of Food Safety, along with Luke LaBorde, professor of food science, will use the funding from USDA’s National Institute of Food and Agriculture to conduct research on the interactions between microorganisms found in fruit-packing environments and Listeria monocytogenes.

“We will study the ability of environmental microorganisms to form robust biofilms together with L. monocytogenes and how these biofilms may protect L. monocytogenes from the antimicrobial activity of sanitizers,” said Kovac, assistant professor of food science. “The data generated in this project will help improve the cleaning and sanitizing used in the fresh produce industry to better control L. monocytogenes and support the production of safe food.”

Listeria and other microorganisms found in the natural environment, such as soil, can be introduced unintentionally into food-processing facilities with raw foods such as fruit. The research is needed, Kovac explained, because once introduced into the food-processing environment, Listeria and many other environmental microorganisms can grow on surfaces into microbial layers called biofilms.

“Microorganisms enclosed in a biofilm produce slimy substances that protect them from the antimicrobial activity of sanitizing chemicals by slowing down their penetration into the core of a biofilm,” Kovac said. “Biofilm formation can therefore result in reduced efficacy of antimicrobial sanitizers used to inactivate Listeria. This project will investigate the interactions between microorganisms found in fruit-packing environments and L. monocytogenes.”

Research – Determination of Listeria monocytogenes, competitive microflora, microbial hygiene indicators and physico-chemical parameters during the shelf-life of the typical Mediterranean style fermented sausage “Salsiccia Sarda”

Journal of Food Protection

The aim of the present study was the determination of Listeria monocytogenes , competitive microbiota, microbial hygiene indicators and physico-chemical parameters in the typical Mediterranean Style fermented sausages “Salsiccia Sarda” . A batch of “Salsiccia Sarda” (25 samples) naturally contaminated by L. monocytogenes and vacuum packaged after 24 days of ripening was included in the study. Fifteen samples stored at 8° C were analysed after 13, 90 and lastly at the end of shelf-life, after 180 days from vacuum packaging. Ten vacuum packaged samples were stored at 12°C in a domestic fridge simulating temperature abuse and were evaluated at the end of the shelf-life. Samples were subjected to physico-chemical analysis (pH and aw) and investigated for the presence and enumeration of L. monocytogenes . Competitive microbiota, Lactic Acid Bacteria (LAB) and Coagulase negative Staphylococci (CNS), and microbial hygiene indicators (Total mesophilic bacterial counts, Enterobacteriaceae, Enterococcuss spp. and Staphylococcus aureus ) were determined in all the samples. Although a decreasing trend in L. monocytogenes prevalence was observed through the shelf-life, the detection of the pathogen in fermented sausages confirms the ability of L. monocytogenes to overcome the hurdles of the manufacturing process. The results of the present study highlight the importance to carefully evaluate the “Salsiccia Sarda” production process by Food Business Operators (FBOs) in order to maintain unfavourable conditions for the growth of L. monocytogenes .