Category Archives: Food Technology

Research – Scale‐up model of forced air‐integrated gaseous chlorine dioxide for the decontamination of lowbush blueberries

Posted on March 27, 2020 by | Leave a comment

Wiley Online 

Gaseous chlorine dioxide (ClO2) is a promising sanitizer for frozen products because of its efficacy under nonthermal and waterless conditions. A major knowledge gap exists between laboratory trials and effectiveness at the industrial scale. To address this, a pilot study implementing a pallet‐sized fumigation container (60 harvest totes) was designed for gaseous ClO2. Fifty kilograms of blueberries were exposed to initial dose of 57.46 mg/L, representing a treatment of 2.35 mg/g of blueberries. Blueberries remained enclosed for 10 hr. Reduction of all viable cells, coliforms, yeasts, and molds were measured by plating treated samples on Tryptic Soy Agar, Violet Red Bile Agar, and Dichloran Rose Bengal Chloramphenicol Agar and compared to untreated controls. The results demonstrate that a significant reduction of 1.5 log CFU/g can be achieved against coliforms after ClO2 exposure. Our findings demonstrate a cost‐effective procedure that could be adapted to commercial processing.

Leave a comment

Posted in Coliforms, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, microbial contamination, Microbiology, mold, Moulds, Research, Technology, Uncategorized, Yeasts

UK – FSA publishes guidance for food businesses on coronavirus (COVID-19)

Posted on March 26, 2020 by | Leave a comment

FSA

The FSA has today published guidance to assist food businesses in responding to the coronavirus (COVID-19) outbreak.

The new guidance has been developed with the Department for Environment, Food and Rural Affairs (Defra) and covers a range of areas including good hygiene practice, management of employee sickness, and social distancing for specific food business settings.

It is very unlikely that people can catch COVID-19 from food. COVID-19 is a respiratory illness and not known to be transmitted by exposure to food or food packaging.

The FSA is working with the food industry to ensure that businesses know what their responsibilities are and what actions they need to take to maintain safety standards and protect staff during the outbreak.

The guidance can be found on GOV.UK

Leave a comment

Posted in Coronavirus, COVID-19, food contamination, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Safety, Food Standards Agency, Food Technology, Food Testing, Uncategorized

Research – Modeling the inactivation of Bacillus cereus by infrared radiation in paprika powder (Capsicum annuum)

Posted on March 26, 2020 by | Leave a comment

Wiley Online 

Infrared (IR) irradiation, a novel technology for modeling of decontamination of Bacillus cereus in paprika powder was evaluated and the effect on temperature profiles and total phenolic content was determined. The highest reduction in B. cereus count (2.3 log CFU/g) was achieved after a holding time of 1 min at 200 W IR power and 5 cm distance. The rapid rise in temperature was observed in surface paprika powder and the highest temperature at 200 W IR power and 5 cm distance reaching to 127.8°C. An increase in IR power and a decrease in sample distance of the IR lamp caused a significant decrease in the total phenolic content. The Double Weibull model closely predicted the inactivation of B. cereus in paprika powder by IR irradiation.

Leave a comment

Posted in Bacillus, Bacillus cereus, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, microbial contamination, Microbiology, Research, Technology, Uncategorized

Research – Eliminating viruses in our food with cranberries and citrus fruit

Posted on February 29, 2020 by | Leave a comment

Science Daily

Fresh produce is a major vehicle for noroviruses, a group of viruses that are the most common cause of gastroenteritis in developed countries. However, the viruses are quite resistant to cold pasteurization treatments such as irradiation, which are used to destroy bacteria, moulds, parasites, and insects. The irradiation process uses gamma rays or X-rays to destroy these viruses but at the dose needed to eliminate them, it can affect the physicochemical properties of fresh produce.

Professor Monique Lacroix, a researcher at Institut National de la Recherche Scientifique (INRS), has developed an edible coating based on cranberry juice and citrus extract that makes noroviruses more sensitive to gamma irradiation, making it possible to eliminate them while preserving the quality of food, all without risk to consumers.

Leave a comment

Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Technology, Food Virus, microbial contamination, Microbiology, Norovirus, Research, Technology, Uncategorized, Virus

Research – Effects of coating pistachio kernels with mixtures of whey protein and selected herbal plant extracts on growth inhibition of Aspergillus flavus and prevention of aflatoxin during storage

Posted on February 17, 2020 by | Leave a comment

Wiley Online

Whey protein concentrate (WPC) mixed with various concentrations of Shirazi thyme (ST), sage, and cumin seed (CS) extracts separately. Then pistachio kernels (PK) contaminated with Aspergillus flavus (Af) were coated with each extract and the Af mycelium and generated aflatoxins measured after 3, 5, and 7‐days at 20°C. The ST, sage, and CS had two major antioxidants of thymol (~27%) and carvacrol (~41%), α‐thujone (~28%) and camphor (~14%), and cumin‐aldehyde (~21%) and safranal (~20%), respectively. While the Af mycelium diameter on PK without extract became >90 mm within 3 days, it was shrunk after 7 days when the WPC‐coated PK had 4,000 ppm ST extract. When ST concentrations increased in WPC‐coated PK linearly, the Af growth and aflatoxins production decreased logarithmically. No aflatoxins (B1, B2, G1, and G2) detected in PK after 9 days when the extract concentrations of ST, sage, and CS in WPC reached, respectively to 5,000, 4,500, and 6,500 ppm. Sage extract had significantly (p < .01) the highest TPC (lowest IC50) and preventing power for aflatoxin generation in comparison with ST and CS extracts. The PK will be safe and healthy if the extract concentration of sage, ST, and CS exceed 950, 1,400, and 1,700 mg/kg, respectively.

Leave a comment

Posted in Aflatoxin, Aspergillus, Food Micro Blog, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, Food Toxin, microbial contamination, Microbiology, Mould Toxin, Mycotoxin, Research, Uncategorized

Research -An in situ-Synthesized Gene Chip for the Detection of Food-Borne Pathogens on Fresh-Cut Cantaloupe and Lettuce

Posted on February 15, 2020 by | Leave a comment

Frontiers

Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific sequences of Salmonella Typhimurium, Vibrio parahemolyticus, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7 from the National Center for Biotechnology Information database. Tiling array probes were designed to target selected genes in an optimized hybridization system. A total of 141 specific probes were selected from 3,227 hybridization probes, comprising 26 L. monocytogenes, 24 S. aureus, 25 E. coli O157:H7, 20 Salmonella Typhimurium, and 46 V. parahemolyticus probes that are unique to this study. The optimized assay had strong amplification signals and high accuracy. The detection limit for the five target pathogens on fresh-cut cantaloupe and lettuce was approximately 3 log cfu/g without culturing and with a detection time of 24 h. The detection technology established in this study can rapidly detect and monitor the foodborne pathogens on fresh-cut fruits and vegetables throughout the logistical distribution chain, i.e., processing, cleaning, fresh-cutting, packaging, storage, transport, and sale, and represents a valuable technology that support the safety of fresh agricultural products.

Leave a comment

Posted in E.coli, E.coli O157, E.coli O157:H7, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, Listeria, Listeria monocytogenes, microbial contamination, Microbiology, Research, Staphylococcus aureus, Technology, Uncategorized, Vibrio, Vibrio parahaemolyticus

Research – Fate of Listeria on various food contact and noncontact surfaces when treated with bacteriophage

Posted on February 15, 2020 by | Leave a comment

Wiley Online

Study objective was to determine efficacy of a bacteriophage suspension against Listeria spp. when applied to three common types of materials used in food manufacturing facilities. Materials included two food contact materials (stainless steel and polyurethane thermoplastic belting) and one noncontact material (epoxy flooring). Coupons of each material were inoculated with a cocktail containing L. monocytogenes and L. innocua (4 to 5‐log10 CFU/cm2). Two phage concentrations and a control, 0, 2 × 107 and 1 × 108 PFU/cm2 were evaluated. Treated samples were held at 4 or 20°C for 1 and 3 hr to determine the effect of temperature and treatment time. Reductions in Listeria populations ranged from 1.27 to 3.33 log10 CFU/cm2 on stainless steel, from 1.17 to 2.76 log10 CFU/cm2 on polyurethane thermoplastic belting, and from 1.19 to 1.76 log10 CFU/cm2 on epoxy resin flooring. Higher phage concentration (1 × 108 PFU/cm2), longer treatment time (3 hr), and processing area temperature of 20°C showed a greater (p ≤ .05) reduction of Listeria on the stainless‐steel and polyurethane thermoplastic belting coupons. Overall, Listeria reduction by phage treatment occurred on all three materials tested, under all conditions.

Leave a comment

Posted in Bacteriophage, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, Listeria, Listeria monocytogenes, microbial contamination, Microbiology, Research, Uncategorized

Research – Decontamination of Bacillus cereus in cardamom (Elettaria cardamomum) seeds by infrared radiation and modeling of microbial inactivation through experimental models

Posted on February 11, 2020 by | Leave a comment

Wiley Online

In this work, infrared (IR) irradiation was used for inactivation of Bacillus cereus in cardamom seeds. The effect of IR power (100, 200, and 300 W), sample distance from radiation source (5, 10, and 15 cm) and holding times (0–11 min) was investigated on B. cereus count, as well as cardamom seeds color and temperature profiles. Inactivation of B. cereus on cardamom seeds during IR processing was demonstrated by experimental models. The highest reduction of B. cereus count (5.11 Log CFU/g) was achieved after 8 min IR irradiation at 300 W power and 15 cm distance. Measurement of temperature profiles revealed that there was a significant difference (p < .05) between surface and center temperatures of the cardamom seeds. The green color (a* value) of cardamom seeds was slightly affected and the highest color change was observed at 200 W IR, 10 cm distance and 10 min irradiation that resulted in an increase of a* from −3.05 ± 0.96 to −0.05 ± 0.44. In conclusion, IR irradiation could be successful for decontamination of cardamom seeds without severe alteration of its quality. Among the experimental models for microbial inactivation during IR processing, the Double Weibull model had the highest coefficient value of determination (R2 = 0.9966).

Leave a comment

Posted in Bacillus, Bacillus cereus, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Technology, microbial contamination, Microbiology, Uncategorized

Research – Prediction of Salmonella presence and absence in agricultural surface waters by artificial intelligence approaches

Posted on February 11, 2020 by | Leave a comment

Wiley Online Salmonella kswfoodworld

The purpose of this study was to evaluate the performance of artificial intelligence tools for the prediction of Salmonella presence and absence in agricultural surface waters based on the population of microbiological indicators (total coliform, generic Escherichia coli, and enterococci) and physicochemical attributes of water (air and water temperature, conductivity, ORP, pH, and turbidity). Previously collected data set from six agricultural ponds monitored for two growing seasons were used for analysis. Classification algorithms including artificial neural networks (ANNs), the nearest neighborhood algorithm (kNN), and support vector machines (SVM) were trained and tested with a 539‐point data set for optimum prediction accuracy. Classification accuracy performances were validated with data set (400 samples) collected from different agricultural surface water sources. All tested algorithms yielded the highest accuracy around 75 ± 1% for generic E. coli followed by enterococci (65 ± 5%) and total coliform (60 ± 10%). Classifiers calculated 6–15% higher accuracy ranging from 62 to 66% for turbidity than all other tested physicochemical attributes. Based on E. coli populations measured in other water sources, trained algorithms predicted the presence and absence of Salmonella with an accuracy between 58.15 and 59.23%. The classification performance of ANN, kNN, and SVM algorithms are encouraging for the prediction of Salmonella in agricultural surface waters.

Leave a comment

Posted in Contaminated water, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Technology, microbial contamination, Microbiology, Salmonella, Uncategorized, Water, water microbiology, Water Safety

Research – A Successful Technique for the Surface Decontamination of Salmonella enterica Serovar Typhimurium Externally Contaminated Whole Shell Eggs Using Common Commercial Kitchen Equipment

Posted on February 10, 2020 by | Leave a comment

Mary Ann Liebert hazegg.jpg

Eggs are a highly nutritious food source used in a wide range of food products. In Australia, eggs are a frequent source of foodborne salmonellosis outbreaks, associated with eggshell contamination with Salmonella enterica serovar Typhimurium (ST). Despite their potentially hazardous nature, raw eggs are often used and consumed in mayonnaise, mousse, ice cream and eggnog. The aim of this study was to develop a shell egg decontamination method that removed ST contamination from the outside of an egg without impacting its usability. The decontamination method was developed by the adaptation of a temperature-controlled water bath (commonly present in kitchens and associated with the sous-vide technique) for the surface decontamination of eggs. The outside of whole eggs was artificially inoculated with two ST strains. The eggs were decontaminated by placing in a sous-vide cooker with the water heated to 57°C. The remaining viable ST present on the whole shell egg, crushed shells, internal egg contents and sous-vide water were enumerated over time by culturing onto XLD agar. The quality of the uncontaminated heat-treated eggs was determined by measuring the Haugh unit, yolk index, albumen pH, thermocoagulation, and stability of foam. A blind control study was conducted to assess the acceptability and usability of the treated eggs by chefs and food handlers for the preparation of mayonnaise. Complete decontamination of ST was achieved by treating eggs for 9 min in the sous-vide cooker (57°C). No statistically significant difference was observed in the quality of treated eggs compared with nontreated eggs using the quality measurements and acceptability score from chefs. This method provides a simple approach that can be adopted by chefs and food handlers to obtain safe eggs before the preparation of raw egg products.

Leave a comment

Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, microbial contamination, Research, Salmonella, Technology, Uncategorized