Category Archives: E.coli

USA – Outbreak of E. coli Infections – Latest Outbreak Information

CDC

Latest Outbreak Information
Illustration of a megaphone.
At A Glance
  • As of April 4, 2019, 72 people infected with the outbreak strain of E. coli O103 have been reported from five states.
    • Eight people have been hospitalized. No cases of hemolytic uremic syndrome (HUS) or deaths have been reported.
  • This investigation is still ongoing and a specific food item, grocery store, or restaurant chain has not been identified as the source of infections.
  • CDC is not recommending that consumers avoid any particular food at this time. Restaurants and retailers are not advised to avoid serving or selling any particular food.
  • This is a rapidly evolving investigation. We will update our advice if a source is identified.

Research – An approach to improve the safety and quality of ready‐to‐eat blueberries

Wiley Online

Abstract

Bioactive edible coatings were developed and applied to blueberries as a natural treatment. E. coli O157:H7, L. innocuaS. aureus, and P. aeruginosa were subjected to four bioactive compounds and to three film‐forming solutions (FFS). Vanillin and geraniol at low concentrations (1.2–1.8 mg/mL and 0.4–1 μL/mL) demonstrated significant inhibitory effects on all pathogens counts. Chitosan (Ch) showed a high antimicrobial activity (final counts below 2 log CFU/mL). The effectiveness of Ch plus vanillin (Ch‐Va) and geraniol (Ch‐Ge) in improving the safety were tested against pathogens inoculated on blueberries. Ch, Ch‐Va, and Ch‐Ge coatings exerted a bactericidal effect on all pathogens (from 1.24 to more than 2 log reductions). Significant reduction in yeast and mold counts was achieved with Ch (1.09 log) and Ch + Va (1.74 log). Sensory attributes of blueberries remained acceptable. Ch‐Va and Ch‐Ge were an alternative to improve the quality and safety and could be effective in extending the shelf life of ready‐to‐eat blueberries.

Practical applications

The present study proposes the use of natural coatings enriched with biopreservatives as a technological alternative to enhance the quality and safety of minimally processed fruits. According to the results obtained, the application of chitosan plus vanillin/geraniol coatings on fresh blueberries would allow to offer a safe product and respond the growing demand of consumers for fresh, environmentally friendly and chemical preservatives‐free foods. These findings and those obtained by the sensory evaluation support the practical application of this alternative in the minimally processed fruit industry.

Research – Inactivation of Staphylococcus aureus and Escherichia coli in milk by different processing sequences of ultrasound and heat

Wiley Online

Abstract

Different processing sequences of ultrasound and heat were applied for the inactivation of Staphylococcus aureus and Escherichia coli in milk. The changes in whey protein content, particle size, zeta potential, pH, viscosity, and color values were studied to evaluate the effects on the quality of milk. Results indicated ultrasound treatment (600 W, 5 min) with postheating (63 °C 5 min) reduced S. aureus and E. coli by 1.58 and 2.02 log cfu/ml, respectively, but yielded 0.26 and 0.19 log cfu/ml sublethal S. aureus and E. coli. Milk preheated to 63 °C (5 min) followed by ultrasound (600 W, 5 min) showed 1.17 and 1.68 log reductions for S. aureus and E. coli, respectively, and the sublethal S. aureus and E. coliwere reduced to 0.16 and 0.09 log cfu/ml. The increased cavitation effects make the simultaneous treatment (600 W, 63 °C, 5 min) the most effective approach in killing S. aureus and E. coli, causing 1.67 and 2.16 log reductions, respectively, and producing negligible sublethal bacteria. The simultaneous treatment was also able to improve some physical–chemical properties of milk; smaller particle size and whiter color were achieved. However, whey protein and stability indices of milk were slightly deteriorated by this approach.

Practical applications

In food processing fields, thermosonication can serve as an alternative processing technique to thermal pasteurization. Especially, the simultaneous treatment of ultrasound and heat showed best inactivation effect for viable and sublethal cells, while maintaining the overall milk quality in the meantime. The results of this research may be useful for the treatment of various bacterial contamination and give the guidance for milk processing

Research – Microbial risks in food franchise: A step forward in establishing ideal cleaning and disinfection practices in SSOPs

Wiley Online

Abstract

Microbial contamination in coffee specialty franchises using uniform processing procedures in Korea was examined. Cleaning or disinfection practices for sanitation standard operating procedures (SSOPs) are proposed. For each processing step, food materials and associated processing environments were tested for heterotrophic bacteria (HB), Escherichia coli, coliform, and Staphylococcus aureus. Existing SSOP cleaning or disinfection practices were also evaluated. Ice‐making procedures revealed coliform and HB contamination in all coffee houses, with high HB contamination on several supplementary food materials and associated food utensils. Microbial loads in food materials or final products were high on structures and materials that are difficult to clean and disinfect. Staphylococcus species contaminated food machinery and utensils directly contacted by food handlers. Based on our analysis of cleaning and disinfection practices in each franchise’s SSOPs, current practices should be complemented to ensure food safety. Our results provide a foundation for developing sanitation standards optimized for coffee specialty franchises.

Practical applications

Franchise brands operating collectively require well‐established sanitation practices such as sanitation standard operating procedures (SSOPs) to ensure food safety and quality. Microbiological studies were performed to evaluate coffee processing procedures and environments at specialty franchise brands. Hygiene practices in SSOPs were simultaneously evaluated to examine cleaning and disinfection procedures. In several processing procedures and associated processing environments, coliform and Staphylococcus aureus were detected on food handlers’ hands, gloves, and hand‐accessible areas of food utensils or machinery simultaneously. Coliforms were also detected in edible ice cubes and on related utensils or machinery for ice production or storage (ice‐making machine, ice‐bin). Heterotrophic bacteria (HB) were detected at high densities in sugar syrup (under‐using) and edible ice (during production and storage). Particularly, HB concentrations increased during processing. Thus, microbial contamination is increased by various factors during processing, particularly inappropriate cleaning or disinfection of utensils or machinery. Evaluation of cleaning and disinfection practices for each franchise’s SSOPs revealed that the cleaning and disinfection practices for machinery or utensils accessible to worker’s hands must be supplemented. Establishments serving a wide variety of coffee beverages may require complex and different processing procedures. Because utensils and machinery affecting the microbial load of the final product can vary, the areas and targets of cleaning and disinfection should be expanded. Second, the structure or material characteristics of food utensils or machinery that are difficult to clean and disinfect can lead to microbial growth. Cleaning or disinfection of food utensils or machinery should be considered in SSOPs. Purchasing management (as a major category of SSOPs for food safety) should be expanded to utensils or machinery in addition to food materials. Furthermore, cleaning and disinfection targets aimed at under‐used utensils or facilities must be established in SSOPs.

RASFF Alerts – STEC E.coli – Frozen Parsley – Frozen Boneless Beef

RASFF-Logo

RASFF – shigatoxin-producing Escherichia coli (stx+ /25g) in frozen boneless beef from Brazil in Spain

RASFF – enteropathogenic Escherichia coli (stx2+; eae+) in frozen parsley from Germany in Germany

RASFF Alert – E.coli – Frozen Venison Goulash

RASFF-Logo

RASFF – lead (0.3; 0.38 mg/kg – ppm) and high count of Escherichia coli (29000 CFU/g) in frozen venison goulash from Spain in Austria

USA – Kentucky E. coli O103 Outbreak Rises to 44 Sick, TN, OH, GA Included

Food Poisoning Bulletin

The Kentucky E. coli O103 outbreak has now risen to 44 sick, as of April 3, 2019, according to news reports. Six people have been hospitalized in this outbreak, but there is no mention if any have developed hemolytic uremic syndrome (HUS), a type of kidney failure that can cause strokes. And there are 20 pending cases that are not yet lab-confirmed.

Research – Increased prevalence of Escherichia coli strains from food carrying blaNDM and mcr-1-bearing plasmids that structurally resemble those of clinical strains, China, 2015 to 2017

Eurosurveillance

Antimicrobial resistance poses an increasing risk to human and animal health worldwide. In particular, carbapenem resistance mediated by serine β-lactamases and metallo-β-lactamases (MBLs), such as the OXA enzymes produced by  and  carbapenemase (KPC-1) and New Delhi metallo-β-lactamase (NDM-1) produced by Enterobacteriaceae, is associated with a high mortality rate among hospitalised patients [1,2]. NDM-1, a type of Ambler class B metallo-β-lactamases (MBLs), exhibits high hydrolytic activity against almost all known β-lactam antimicrobials (except aztreonam), including the last-line carbapenems [3,4]. It was first found to be produced by  and  strains isolated from a Swedish patient of Indian origin who was admitted to hospital in New Delhi, India [5]. Thereafter, the   gene disseminated in various countries and regions such as China, the Middle East, South East Asia and Europe [4]. This multidrug resistance gene, which may be located on either plasmids or chromosome [3,6,7], leaves few therapeutic options for infected patients. In China, Ho et al. reported the first isolation of  -positive  from a 1-year-old infant and its mother in 2011 [8]. NDM-1-producing Enterobacteriaceae have since disseminated to various provinces in China, with the majority of such strains isolated from stool samples [9]. However, reports of isolation of carbapenem-resistant Enterobacteriaceae (CRE) from food samples remain scarce around the world.

USA – E. coli O103 Outbreak in Kentucky, Tennessee, Ohio, and Indiana

Food Poisoning Bulletin

CDC E.coli

Image CDC

The E. coli O103 outbreak originally announced in Kentucky yesterday  apparently also has sickened people living in Tennessee, Ohio, and Indiana, according to news reports. Twenty confirmed cases are in Kentucky, and one each in the other states for a total of 23 ill. Most of the illnesses occurred between March 5 and 25, 2019.

Research – Effects of Sodium Chloride or Calcium Chloride Concentration on the Growth and Survival of Escherichia coli O157:H7 in Model Vegetable Fermentations

Journal of Food Protection

Salt concentration has long been considered an important factor for the quality of fermented vegetable products, but the role of salts in bacterial growth and death during vegetable fermentation remains unclear. We compared the effects of various sodium chloride (NaCl) concentrations, including 1 M (6%) NaCl used in commercial cucumber fermentations and 0.34 M (2%) NaCl used in cabbage and other ready-to-eat vegetable fermentations, on the growth and death of lactic acid bacteria (LAB) of the genus Lactobacillus and pathogenic Escherichia coli (Shiga toxin–producing E. coli, or STEC) strains. We also investigated calcium chloride (CaCl2) salt conditions. CaCl2 is being used at 0.1 M (1.1%) in low-salt commercial cucumber fermentations that lack added NaCl. STEC strains have previously been shown to be among the most acid-resistant pathogens in fermented or acidified vegetables. The data showed that 1.1% CaCl2, and especially 1% NaCl, had a stimulatory effect on the growth rates of STEC and LAB compared with a no-salt control, but higher NaCl concentrations decreased growth rates for STEC; to a lesser extent, LAB growth rates were also reduced. For most salt concentrations tested, maximum cell densities achieved during growth of STEC were reduced compared with those of the no-salt controls, whereas LAB mostly had cell densities that were similar to or greater than those of the no-salt controls. No consistent pattern was observed when comparing death rates with salt type or concentration for the STEC or LAB cocktails undergoing lactic acid stress (50 or 350 mM, respectively) at pH 3.2 and when comparing STEC survival in competitive culture experiments with LAB. For vegetable fermentation safety concerns, the results suggest that an important effect of salt addition is enhancement of the growth of LAB compared with STEC strains. Further research will be needed to determine factors influencing STEC survival in competition with LAB in vegetable fermentations.