Category Archives: E.coli O103

RASFF Alert- STEC E.coli – 026 – 0103 – Frozen Minced Beef

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RASFF – enteropathogenic Escherichia coli (O26:H11 O103:H2 eae+ stx- /25g) in frozen minced beef from the United Kingdom in France

Research – Detection, Isolation, and Characterization of Shiga Toxin–Producing Escherichia coli in Flour

Journal of Food Protection

Wheat flour has recently been described as a novel vehicle for transmission of Shiga toxin–producing Escherichia coli (STEC). Very recently, an outbreak of STEC O121 and STEC O26 infections was linked to flour in the United States. The aim of the present study was to generate baseline data for the occurrence of STEC in flour samples from different retailers in Switzerland. In total, 70 flour samples were analyzed. After enrichment, the samples were screened for stx1 and stx2 by the Assurance GDS MPX ID assay. STEC strains were isolated and serotyped by the E. coli SeroGenoTyping AS-1 kit. The determination of stx subtypes was performed with conventional PCR amplification. Screening for eae, aggR, elt, and estIa/Ib was performed by real-time PCR. Nine (12.9%) of the flour samples tested positive for stx by PCR. STEC was recovered from eight (88.9%) of the positive samples. Two isolates were STEC O11:H48 harboring stx1c/stx1d, two were O146:H28 containing stx2b, one was O103:H2 containing stx1a and eae, and three were O nontypeable: Ont:H12 (stx2a), Ont:H14 (stx2a/stx2g), and Ont:H31 (stx1c/stx1d). STEC O103 belongs to the “top five” serogroups of human pathogenic STEC in the European Union, and STEC O146 is frequently isolated from diseased humans in Switzerland. Our results show that flour may be contaminated with a variety of STEC serogroups. Consumption of raw or undercooked flour may constitute a risk for STEC infection.

Research -Microbiological Testing Program for E. coli O157:H7 and non-O157 Shiga Toxin-Producing E. coli: Individual Positive Results for Raw Ground Beef (RGB) and RGB Components

USDA

Table 1. Raw Ground Beef Products (RGB) Analyzed for E. coli O157:H7, Current Calendar Year

Sample Source1 Collection Date Where Collected Product Status Positives this Year Samples Analyzed this Year Total Positives2 Total Samples Analyzed2
Federal RGB Verification, Beef Oct 15, 2018 MN Held 4 9,541 540 238,301
Federal RGB Verification, Beef Oct 9, 2018 NC Held 3 9,297 539 238,057
Federal RGB Verification, Beef Mar 26, 2018 OR Held 2 3,085 538 231,845
Federal RGB Verification, Beef Feb 8, 2018 CA Held 1 1,704 537 230,464

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Microbiological Testing Program for E. coli O157:H7 and non-O157 Shiga Toxin-Producing E. coli: Individual Positive Results for Raw Ground Beef (RGB) and RGB Components

View by Year:  
2018 Positive Results
2017 Positive Results
2016 Positive Results
2015 Positive Results
2014 Positive Results
2013 Positive Results
2012 Positive Results
2011 Positive Results
2010 Positive Results
2009 Positive Results
2008 Positive Results
2007 Positive Results
2006 Positive Results
2005 Positive Results
2004 Positive Results
2003 Positive Results
2002 Positive Results
2001 Positive Results

The table below includes all positive results as of November 4, 2018.

Table 1. Raw Ground Beef Products (RGB) Analyzed for E. coli O157:H7, Current Calendar Year 

Sample Source1 Collection Date Where Collected Product Status Positives this Year Samples Analyzed this Year Total Positives2 Total Samples Analyzed2
Federal RGB Verification, Beef Oct 15, 2018 MN Held 4 9,541 540 238,301
Federal RGB Verification, Beef Oct 9, 2018 NC Held 3 9,297 539 238,057
Federal RGB Verification, Beef Mar 26, 2018 OR Held 2 3,085 538 231,845
Federal RGB Verification, Beef Feb 8, 2018 CA Held 1 1,704 537 230,464

1Sample Sources may include these types of establishments and samples:

  • Federal (verification; follow-up)
  • Retail (verification; follow-up)
  • State (verification; follow-up)
  • Import (verification; follow-up). For Import samples, the column “Where Collected” is defined as Country of Origin. <!–
  • Source may also refer to the type of product (beef, veal, or mixed), as listed on the product label.
  • –>

2Totals: “Total Positives” and “Total Samples Analyzed” are the totals since FSIS began its testing program to detect E. coli O157:H7 in raw ground beef on October 17, 1994.


The table below includes all positive results as of November 4, 2018.

Table 2. Raw Ground Beef Components (RGBC) Analyzed for Target STECs, Current Calendar Year3

Sample Source4 Collection Date Target STECs Where Collected Product Status Posi-
tives this Year
Samples Analyzed this Year5 Total Posi-
tives
Total Samples Analyzed6
Trim Verification, Beef Oct 18, 2018 O111 PA Held 19 6,594 415 59,239
Trim Verification, Beef Oct 9, 2018 O157:H7 SD Held 18 6,262 414 58,907
Trim Verification, Beef Sep 5, 2018 O157:H7 PA Held 17 5,641 413 58,286
Trim Verification, Beef Aug 14, 2018 O103 PA Held 16 5,018 412 57,663
Trim Verification, Beef Jun 6, 2018 O157:H7 MO Held 15 3,583 411 56,228
Trim Verification, Veal Jun 6, 2018 O26 PA Held 14 3,428 410 56,073
Follow-up to RGBC Positive, Beef May 30, 2018 O103 PA Held 13 3,266 409 55,911
Follow-up to RGBC Positive, Beef May 29, 2018 O103 PA Held 12 3,266 408 55,911
Trim Verification, Beef May 21, 2018 O157:H7 TX Held 11 3,147 407 55,792
Trim Verification, Beef May 16, 2018 O103 PA Held 10 2,968 406 55,613
Trim Verification, Beef May 15, 2018 O103 NY Held 9 2,968 405 55,613
Trim Verification, Beef May 9, 2018 O103 PA Held 8 2,968 404 55,613
Other RGBC Verification May 9, 2018 O157:H7 NE Held 7 2,968 403 55,613
Trim Verification, Beef Mar  19, 2018 O157:H7 NM Held 6 1,814 402 54,455
Trim Verification, Beef Mar 1, 2018 O121 ID Held 5 1,507 401 54,148
Trim Verification, Veal Feb 27, 2018 O103 WA Held 4 1,347 400 53,988
Follow-up to RGBC Positive, Beef Jan 27, 2018 O45 WI Held 3 583 400 53,225
Other RGBC Verification Jan 4, 2018 O157:H7 SD Held 2 107 399 52,749
Trim Verification, Beef Dec 28, 2017 O157:H7 WI Held 1 107 398 52,749

Research – USA – Microbiological Testing Program for Escherichia coli O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC)

FSIS USDA USDA

FSIS considers raw, non-intact beef products or the components of these products found to have six Shiga toxin-producing Escherichia coli (STEC) to be adulterated, in addition to E. coli O157:H7. (Refer to the Federal Register notice Shiga Toxin-Producing Escherichia coli in Certain Raw Beef Products | PDF). These six non-O157 STECs are O26, O45, O103, O111, O121, and O145.

On June 4, 2012, FSIS began verification testing for these non-O157 STEC in domestic and imported beef manufacturing trimmings from cattle slaughtered on or after June 4, 2012. Beef manufacturing trimmings collected from cattle slaughtered before June 4, 2012, or that contain other components such as cheek meat are analyzed for E. coli O157:H7 only.

USA – E. coli O103 Prompts Meat Recall

Food Poison Journal

Texas Natural Meats, a Lott, Texas establishment, is recalling approximately 489 pounds of frozen raw, ground beef products that may be contaminated with Shiga toxin-producing E. coli (STEC) O103, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.The frozen raw, ground beef items were produced on Aug. 8, 2017.

Holland – Research on pathogens in dairy goat and dairy sheep farms

RIVM 

Synopsis

Animals can carry pathogens that can cause disease in humans (zoonoses). In 2016, the RIVM and the NVWA investigated whether dairy goats and dairy sheep carry such pathogens; sometimes this is also done for livestock farmers, their family members and employees. These pathogens usually cause diarrhoea but sometimes the infections are more severe.

Research shows that a few pathogens occur often on dairy goat and dairy sheep farms. These bacteria reside in the intestines of the animals, and are excreted in manure. A small amount of manure is enough to contaminate raw milk or unpasteurised cheese. Visitors to these farms can also become infected if they come into contact with the animals or their environment. Contamination can be prevented by consuming or processing all milk pasteurized. Visitors can reduce the risk of disease by washing their hands if they have been in contact with the animals or their environment.

STEC and Campylobacter bacteria, in particular, were frequently found. STEC was detected at virtually all the farms that were investigated. Campylobacter was detected at 33 percent of the goat farms and 95.8 percent of the sheep farms. These bacteria were found much less often among the farmers and their family members. Listeria was detected less often: at 8.8 percent of the goat farms and 16.7 percent of the sheep farms, and not among people. However, it is a relevant pathogen since unpasteurised soft cheese is the most important source of Listeria infection in humans.

Salmonella was not found at dairy goat farms but was found at 12.5 percent of the dairy sheep farms. On most farms, only a type of Salmonella that is not transmitted to humans was found. ESBL-producing bacteria, which are insensitive to many antibiotics, were detected at 1.7 percent of the goat farms and 4.2 percent of the sheep farms. They were also found in 6.8 percent of the people. This percentage is not higher than for the general population.

RASFF Alert – STEC E.coli – Chilled Deboned Beef Meat

kswfoodworld food safety poisoning

RASFF-shigatoxin-producing Escherichia coli (O103, stx1, eae /25g) in chilled deboned beef meat from Uruguay in Spain

Information – FSA – E.coli Fact Sheet

FSA 

CDC E.coli

Image CDC

 

Escherichia Coli (known as E. coli) is a type of bacteria that can be found in the intestines of animals and humans. Many strains of E. coli are harmless to humans, but some can cause serious illness. Most cases of foodborne illness are caused by a strain known as E. coli O157.

Research – Shiga toxin-producing E. coli found in food

Science Daily 

 

Escherichia coli (E. coli) is a germ that occurs naturally in the gut of mammals and birds, as well as in the human intestinal flora. However, certain E. coli types can cause severe diarrhea in humans. These virulent E. coli types include Shiga toxin-producing E. coli (STEC), also known as Verotoxin-producing E. coli (VTEC).

Their damaging effect is due to the fact that STEC produce toxins known as Shiga toxins (Stx), which can cause disease in the human gut. As the best known STEC representative, an enterohaemorrhagic E. coli (EHEC) strain of the serotype O104:H4 was responsible for numerous severe cases of haemolyticuremic syndrome (HUS) and bloody diarrhea in Germany in 2011, as a result of which 53 people died.

USA- Beef Products Recall – STEC E.coli

E.coli Blog

PFP Enterprises, a Fort Worth, Texas, establishment, is recalling approximately 15,865 pounds of beef products because they may be contaminated with E. coli O103, E. coli O111, E. coli O121, E. coli O145, E. coli O26 and E. coliO45, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.

FSIS personnel became aware of the problem during a Food Safety Assessment when they discovered that beef trim tested presumptive positive for multiple non-O157 Shiga toxin-producing E. coli (STEC) strains through the company’s testing program. The company inadvertently did not carry the test out to confirmation, and not all affected product was held.