Monthly Archives: February 2023

RASFF Alerts – Listeria monocytogenes – French Cheese – Crumbled Blue Cheese – Leverkaas – Polish Chicken

RASFF

Listeria monocytogenes in cheeses from France in Belgium

RASFF

Listeria monocytogenes in crumbled blue cheese from Denmark in Portugal and Spain

RASFF

Listeria Monocytogenes in leverkaas from Belgium in the Netherlands and Spain

RASFF

Salmonella spp., Listeria monocytogenes and Campylobacter spp. in frozen chicken mid wings from Poland in Lithuania

RASFF Alert – STEC E.coli – Frozen Organic Herb mix

RASFF

Verotoxin-producing Escherichia coli (VTEC/STEC) in frozen organic herb mix from France

RASFF Alert – Ochratoxin A – Nutmeg – Shelled sunflower Seeds – Chopped Dried Figs

RASFF

Ochratoxin A in nutmeg from Indonesia in the Netherlands

RASFF

Ochratoxin A in shelled sunflower seeds from China, packaged in Denmark in Poland

RASFF

Ochratoxin A in Turkish chopped dried figs in the Netherlands

RASFF

Ochratoxin A in dried figs from Turkey in Germany

Research – Use of a phage cocktail to reduce the numbers of seven Escherichia coli strains belonging to different STEC serogroups applied to fresh produce and seeds

Wiley Online

Abstract

The aims of this research were to evaluate the effectiveness of a phage cocktail at reducing seven Shiga toxigenic Escherichia coli (STEC) serogroups on different food matrixes: mung bean sprouts (MBP), lettuce, and mung bean seeds (MBS) and to test the phage cocktail effectiveness to reduce E. coli O157 on Romaine and iceberg lettuce. To study the effect of the type of food matrix on the STEC phage cocktail effectiveness, a mixture of seven highly sensitive STEC strains designated as phage propagation strains (PPS) were used to adulterate Romaine lettuce, MBP, and MBS matrixes at a concentration of 105 logs CFU/g. A subsample of the treated MBS was germinated to assess STEC survival. Recovered STEC strains were confirmed using latex agglutination and PCR. To test the phage cocktail effectiveness to reduce E. coli O157:H7 on Romaine and iceberg lettuce, a mixture of four STEC strains (different than phage propagation strains, non-PPS) at both low (103 CFU/g) and high (105 CFU/g) concentrations were used to spike the samples in scaled up trials for the purpose of potential commercialization. Phage treatments including a combination of STEC phage cocktail and chlorinated water treatment were then applied to lettuce in a simulated scaled-up trial. STEC was assessed on the treated samples at different storage time and temperatures (0, 24, 48, and 72 hr at 2, 10 and 25°C). In the food matrix trial, the combination of STEC phage cocktail and chlorinated water-reduced PPS (p < 0.001) STEC on lettuce by 2.1 log10 CFU/g and on MBP by 2.2 log10 CFU/g. However, isolates from all 7 STEC serogroups remained viable after phage treatment in both lettuce and MBP; particularly those associated with serogroup O111, O121, O103, and O145, while only a few colonies of serogroup O26, O45, and O157 were detected. Lettuce adulterated with low levels of 4 non-PPS E. coli O157:H7 (103 CFU/g) achieved a reduction of 2.6–3.2 logs. While a reduction 1.7–2.3 logs was achieved by the phage cocktail when lettuce was inoculated with 105 CFU/g. Overall phage performance was more effective at 2 and 10°C and improved over storage time up to 72 hr. However, for MBS, the phage cocktail was not able to kill any of the STEC populations as all of them recovered during germination.

Belgium – Sesame cream – EXTRA Tahine from the Mira brand – Salmonella

AFSCA

Communication from the FASFC
Product: Sesame cream – EXTRA Tahine from the Mira brand.
Problem: Possible presence of Salmonella.

The FASFC is today recalling consumers of the product “sesame cream – EXTRA Tahine” from the Mira brand.

This recall follows a notification via the RASFF system (European Food and Feed Rapid Alert System) due to the possible presence of Salmonella.

The AFSCA asks not to consume this product and to bring it back to the point of sale where it was purchased.


Product description:

Name: sesame cream – EXTRA Tahine
Brand: Mira
Expiry date (BDD): 16/12/2023
Batch number: 003
Weight: 18kg

The product was sold via various points of sale in Belgium.

For further information, you can contact the FASFC contact point for consumers: 0800/13.550 or pointdecontact@afsca.be .

France – Italian cassata – Listeria monocytogenes

Gov france

Identification information of the recalled product

  • Product category Feed
  • Product subcategory Prepared meals and snacks
  • Product brand name Unbranded
  • Model names or references Italian cassata
  • Identification of products
    Batch
    1
  • Packaging Italian Cassata sold to take away in plastic trays.
  • Marketing start/end date From 01/16/2023 to 01/22/2023
  • Storage temperature Product to be stored at room temperature
  • Further information Italian Cassata sold to take away in plastic trays.
  • Geographic area of ​​sale Whole France
  • Distributors Quadrifoglio

Practical information regarding the recall

  • Reason for recall Mascarpone used for Italian Cassata contaminated with listeria.
  • Risks incurred by the consumer Listeria monocytogenes (causative agent of listeriosis)
    Poisoning

Research – Effect of pesticide application on Salmonella survival on inoculated tomato leaves

Wiley Online

Abstract

Outbreaks of Salmonellosis have been traced to contaminated tomato. The produce production environment poses a risk for Salmonella contamination; however, little is known about the effects of pest management practices on Salmonella during production. The study objective was to evaluate pesticide application on the inactivation of Salmonella on tomato leaves. Thirty greenhouse-grown tomato plants were inoculated with S. enterica serovars Newport or Typhimurium. Inoculation was performed by dipping tomato leaves in an 8-log CFU/mL Salmonella suspension with 0.025% (vol/vol) Silwet L-77 surfactant for 30 s, for a starting concentration of 6–7 log CFU/mL. Plants were treated with one of four pesticides, each with a different mode of action [acibenzolar-S-methyl, copper-hydroxide, peroxyacetic acid (PAA), and streptomycin]. Pesticides were applied at manufacturers’ labeled rate for plant disease management with water as a control treatment. Salmonella was enumerated at 0.125 (3 h), 2, 6, and 9 days post-inoculation (dpi), and counts log-transformed. Growth of Salmonella was not observed. At 2 dpi, PAA and streptomycin significantly reduced surface Salmonella concentrations of inoculated tomato leaves (0.7 and 0.6-log CFU/g, respectively; p ≤ 0.05), while significant Salmonella log reduction occurred in the ground tomato leaves after copper hydroxide treatment (0.8-log CFU/g; p ≤ 0.05), compared to the control. No significant differences in Salmonella populations on tomato leaf surface and in ground leaves were observed from 2 to 9 dpi, regardless of pesticide application. These findings suggest single in-field pesticide applications may not be an effective mitigation strategy in limiting potential Salmonella contamination. Future research, including multiple in-field pesticide applications, or pesticide use in combination with other mitigation strategies, may offer intriguing management practices to limit possible preharvest contamination.

Research – Effects of UV-C Irradiation and Vacuum Sealing on the Shelf-Life of Beef, Chicken and Salmon Fillets

MDPI

Abstract

One-third of the world’s food supply is lost, with meat being a major contributor to this loss. Globally, around 23% of all meat and 35% of all seafood products are lost or wasted. Meats and seafood products are susceptible to microbial spoilage during processing, storage, and distribution, where microbial contamination causes significant losses throughout the supply chain. This study examined the efficacy of UV-C irradiation and vacuum-sealing in preventing microbiological deterioration in beef, chicken, and salmon fillets. The samples were sterilized using a constant UV-C irradiation dose of 360 J/m2 and stored under a reduced pressure of 40 kPa. A microbiological analysis was conducted daily to examine the microbial contamination, which included counting the colonies of Pseudomonas spp., aerobic bacteria, lactic acid bacteria (LAB), Salmonella, and Escherichia coli, as well as monitoring the increase in pH levels. The results demonstrated a statistically significant difference (p > 0.05) in the aerobic bacteria counts between the storage conditions and storage days in all samples, which is a primary indicator of microbial spoilage. In contrast, the differences varied in the Pseudomonas spp. and LAB counts between the storage conditions and storage days, and there was no significant difference (p < 0.05) in the pH levels between the storage conditions. The results indicate that the combination of UV-C irradiation and vacuum sealing effectively inhibits microbial growth and extends the shelf-life of beef, chicken, and salmon fillets by 66.6%.

Research – Post-Cooking Growth and Survival of Bacillus cereus Spores in Rice and Their Enzymatic Activities Leading to Food Spoilage Potential

MDPI

Abstract

Bacillus cereus strains vary in their heat resistance, post-processing survival and growth capacity in foods. Hence, this study was carried out to determine the effect of cooking on the survival and growth of eight B. cereus spores in rice at different temperatures in terms of their toxigenic profiles and extracellular enzyme activity. Samples of rice inoculated with different B. cereus spores were cooked and stored at 4 °C, 25 °C and 30 °C for up to 7 days, 48 h and 24 h, respectively. Out of eight B. cereus strains, four and three spore strains were able to grow at 30 °C and 25 °C post-cooking, respectively. Rapid growth was observed after a minimum of 6 h of incubation at 30 °C. All strains possessed proteolytic activity, whereas lipolytic and amylolytic activities were exhibited by 50% and 12.5% of the strains, respectively. The post-cooking survival and growth capacity of the B. cereus strains appeared to be independent of their toxigenic profiles, whereas extracellular enzymatic activities were required for their vegetative growth. Due to the B. cereus spores’ abilities to survive cooking and return to their active cellular form, great care should be taken when handling ready-to-eat foods.

Research – Extending the Shelf Life of Raw Milk and Pasteurized Milk with Plantaricin FB-2

MDPI

Abstract

Raw milk and pasteurized milk are characterized by a short shelf life, and drinking expired raw milk and pasteurized milk causes illness. In the study, Plantaricin FB-2 (extracted from Lactiplantibacillus plantarum FB-2) was added to liquid milk. By evaluating the microbial growth, acidity changes, protein content, and sensory changes in raw milk and pasteurized milk during storage, it was found that when Plantaricin FB-2 was added at 0.4 g/kg, the shelf life of raw milk was extended by 3 days (7 days if not added). The shelf life of pasteurized milk with Plantaricin FB-2 was extended to 31 days (25 days in the control group), and the optimal amount was 0.3 g/kg. This confirmed that Plantaricin FB-2 can effectively prolong the shelf life of raw and pasteurized milk. This study provides valuable information for the application of bacteriocins produced by Lactiplantibacillus plantarum in raw milk and pasteurized milk to improve their shelf life.