Category Archives: Vibrio parahaemolyticus

Research – Antibacterial Activity of Phloretin Against Vibrio parahaemolyticus and Its Application in Seafood

MDPI

Abstract

Although phloretin is widely utilized in the food industry as an additive, its effects on foodborne pathogens remain insufficiently investigated. This study aimed to evaluate the antimicrobial properties of phloretin (PHL) against Vibrio parahaemolyticus (V. parahaemolyticus) and to elucidate the potential mechanisms of action. After PHL treatment, alterations in the cell morphology, cell microstructure, and intracellular contents of V. parahaemolyticus were assessed. Scanning electron microscopy revealed substantial damage to cell integrity, subsequent to PHL treatment. A notable reduction in intracellular components, including proteins, ATP, and DNA, was observed in samples treated with PHL. PHL was shown to inhibit the activities of ATPase, β-galactosidase, and respiratory chain dehydrogenase in V. parahaemolyticus. Furthermore, it was demonstrated to elevate the intracellular levels of reactive oxygen species and promote cell death. After being applied to sea bass, shrimp, and oysters, PHL effectively inactivated V. parahaemolyticus in these seafoods. These findings demonstrate that PHL has potential for application in seafood to control V. parahaemolyticus.

Research – Vibrio parahaemolyticus Foodborne Illness Associated with Oysters, Australia, 2021–2022

CDC

Abstract

The bacterium Vibrio parahaemolyticus is ubiquitous in tropical and temperate waters throughout the world and causes infections in humans resulting from water exposure and from ingestion of contaminated raw or undercooked seafood, such as oysters. We describe a nationwide outbreak of enteric infections caused by Vibrio parahaemolyticus in Australia during September 2021–January 2022. A total of 268 persons were linked with the outbreak, 97% of whom reported consuming Australia-grown oysters. Cases were reported from all states and territories of Australia. The outbreak comprised 2 distinct strains of V. parahaemolyticus, sequence types 417 and 50. We traced oysters with V. parahaemolyticus proliferation back to a common growing region within the state of South Australia. The outbreak prompted a national recall of oysters and subsequent improvements in postharvest processing of the shellfish.

Research – Vibrio parahaemolyticus Foodborne Illness Associated with Oysters, Australia, 2021–2022

CDC

Abstract

The bacterium Vibrio parahaemolyticus is ubiquitous in tropical and temperate waters throughout the world and causes infections in humans resulting from water exposure and from ingestion of contaminated raw or undercooked seafood, such as oysters. We describe a nationwide outbreak of enteric infections caused by Vibrio parahaemolyticus in Australia during September 2021–January 2022. A total of 268 persons were linked with the outbreak, 97% of whom reported consuming Australia-grown oysters. Cases were reported from all states and territories of Australia. The outbreak comprised 2 distinct strains of V. parahaemolyticus, sequence types 417 and 50. We traced oysters with V. parahaemolyticus proliferation back to a common growing region within the state of South Australia. The outbreak prompted a national recall of oysters and subsequent improvements in postharvest processing of the shellfish.

Research – Climate Change Is Making Europe’s Shellfish Dangerous

Medscape

Climate change is putting Europeans at an increased risk of contracting Vibrio-related infections, according to an assessment recently released by the European Food Safety Authority (EFSA).

Warming sea temperatures are expecting to increase the prevalence of the bacteria in seafood, especially in low-salinity or brackish waters, with a rise in antimicrobial resistance among the species creating additional cause for concern.

Vibrio bacteria, traditionally a problem in tropical and subtropical regions, can cause a range of illnesses, from mild gastroenteritis and ear infections to life-threatening conditions like sepsis.

Experts in Europe are calling for proper surveillance, funded research, and public awareness campaigns.

Taiwan reports 147,000 gastroenteritis cases after Mid-Autumn Festival

Taiwan News

TAIPEI (Taiwan News) — Gastroenteritis cases have continued after the Mid-Autumn Festival, and the Centers for Disease Control (CDC) estimated the outbreak will peak in early October.

The CDC announced Tuesday (Sept. 24) that from Sept.15-21, there were approximately 147,000 medical visits for diarrhea, a 5.7% increase from the previous week, per CNA. A total of 123 cluster cases of diarrhea were reported, marking a five-year high for both medical visits and cluster reports.

CDC Epidemic Intelligence Center Director Guo Hung-wei (郭宏偉) said pathogen tests showed 47 positive cases, with norovirus detected in 28 cases, accounting for 60%, followed by Vibrio parahaemolyticus in 12 cases, making up 25.5%.

The gastroenteritis outbreak typically peaks around the Mid-Autumn Festival each year. CDC Deputy Director Lo Yi-chun (羅一鈞) said that the peak of this diarrhea outbreak is expected to occur around early October.

RASFF Alerts – Vibrio – Prawns

RASFF

Vibrio vulnificus in gambas from Ecuador

RASFF

Vibrio cholerae and parahaemolyticus in shrimps from Ecuador in Romania

Hong Kong – CFS found excessive Vibrio parahaemolyticus in sample of clam in preserved fish sauce.

Gov HK

The Centre for Food Safety (CFS) of the Food and Environmental Hygiene Department said today (September 5) that it is actively following up on food poisoning clusters involving clam in preserved fish sauce, including inspecting the food premises concerned. A sample of clam in preserved fish sauce collected from the food premises concerned was found to contain an excessive amount of Vibrio parahaemolyticus. The CFS is following up on the case.

“Upon notification by the Centre for Health Protection of the Department of Health earlier, the CFS immediately sent staff to the food premises concerned in Mong Kok to conduct an investigation and collected a sample of clam in preserved fish sauce and sent to laboratory for testing. The test result showed that the sample contained Vibrio parahaemolyticus at a level of 1 400 000 per gram. According to the Microbiological Guidelines for Food, if ready-to-eat food contains Vibrio parahaemolyticus at a level of more than 1 000 per gram, it is considered unsatisfactory,” a CFS spokesman said.

The CFS has informed the food premises concerned of the irregularity. The food premises concerned have already stopped selling and discarded the affected product according to the CFS’s advice. The CFS has also provided health education on food safety and hygiene to the person-in-charge and staff of the food premises concerned, and requested that they review and improve the food production process and carry out thorough cleaning and disinfection. Prosecution will be instituted should there be sufficient evidence.

According to section 54 of the Public Health and Municipal Services Ordinance (Cap. 132), all food available for sale in Hong Kong, imported or locally produced, should be fit for human consumption. An offender is subject to a maximum fine of $50,000 and imprisonment for six months upon conviction.

“Vibrio parahaemolyticus, a common bacterium that can cause food poisoning, is naturally present in seawater and often found in seafood. Food poisoning caused by this pathogenic bacterium is usually associated with consumption of undercooked or raw seafood, in particular shellfish, or improperly handled food that is contaminated with bacteria. Common symptoms of food poisoning caused by Vibrio parahaemolyticus, including diarrhoea, vomiting, mild fever and abdominal pain, usually occur within one or two days after consumption of contaminated food. Recovery is usually within a few days. Nonetheless, children, the elderly, pregnant women and those with a weaker immune system are more likely to develop severe symptoms,” the spokesman said.

​The CFS will continue to follow up on the incident and take appropriate action to safeguard food safety and public health.

Ends/Thursday, September 5, 2024
Issued at HKT 18:26

Hong Kong – CHP investigates two food poisoning clusters

Gov HK

The Centre for Health Protection (CHP) of the Department of Health is today (August 30) investigating two epidemiologically linked food poisoning clusters affecting nine persons, and reminded the public to maintain personal, food and environmental hygiene to prevent foodborne diseases.

The first cluster involved two males and four females, aged between 36 and 46, who developed abdominal pain, diarrhoea, nausea, vomiting, and fever about 10 to 15 hours after having dinner at a restaurant in Mong Kok on August 28.

The other cluster involved one male and two females, aged between 28 and 30, who developed similar symptoms about nine to 31 hours after having dinner at the same restaurant on the same day.

Among the nine affected persons, five of them sought medical advice. Two of them required hospitalisation. The stool specimen of one affected person was positive for Vibrio parahaemolyticus upon laboratory testing. All patients are in stable condition.

Initial investigations by the CHP revealed that the affected persons had consumed common food and the food concerned was clam in preserved fish sauce. The incident might have been caused by Vibrio parahaemolyticus.

Personnel from the Centre for Food Safety (CFS) of the Food and Environmental Hygiene Department conducted an inspection at the restaurant, took food sample for examination, and instructed the premises concerned to suspend the sale of relevant food as well as carry out cleaning and disinfection of the premises. The CFS has also provided health education related to food safety and environmental hygiene to the staff of the premises. The investigations of the CHP and the CFS are ongoing.

Research – Public health aspects of Vibrio spp. related to the consumption of seafood in the EU

EFSA

Abstract

Vibrio parahaemolyticus, Vibrio vulnificus and non‐O1/non‐O139 Vibrio cholerae are the Vibrio spp. of highest relevance for public health in the EU through seafood consumption. Infection with V. parahaemolyticus is associated with the haemolysins thermostable direct haemolysin (TDH) and TDH‐related haemolysin (TRH) and mainly leads to acute gastroenteritis. V. vulnificus infections can lead to sepsis and death in susceptible individuals. V. cholerae non‐O1/non‐O139 can cause mild gastroenteritis or lead to severe infections, including sepsis, in susceptible individuals. The pooled prevalence estimate in seafood is 19.6% (95% CI 13.7–27.4), 6.1% (95% CI 3.0–11.8) and 4.1% (95% CI 2.4–6.9) for V. parahaemolyticus, V. vulnificus and non‐choleragenic V. cholerae, respectively. Approximately one out of five V. parahaemolyticus‐positive samples contain pathogenic strains. A large spectrum of antimicrobial resistances, some of which are intrinsic, has been found in vibrios isolated from seafood or food‐borne infections in Europe. Genes conferring resistance to medically important antimicrobials and associated with mobile genetic elements are increasingly detected in vibrios. Temperature and salinity are the most relevant drivers for Vibrio abundance in the aquatic environment. It is anticipated that the occurrence and levels of the relevant Vibrio spp. in seafood will increase in response to coastal warming and extreme weather events, especially in low‐salinity/brackish waters. While some measures, like high‐pressure processing, irradiation or depuration reduce the levels of Vibrio spp. in seafood, maintaining the cold chain is important to prevent their growth. Available risk assessments addressed V. parahaemolyticus in various types of seafood and V. vulnificus in raw oysters and octopus. A quantitative microbiological risk assessment relevant in an EU context would be V. parahaemolyticus in bivalve molluscs (oysters), evaluating the effect of mitigations, especially in a climate change scenario. Knowledge gaps related to Vibrio spp. in seafood and aquatic environments are identified and future research needs are prioritised.

Hong Kong – CHP investigates two food poisoning clusters

Gov HK

  The Centre for Health Protection (CHP) of the Department of Health is today (July 25) investigating two epidemiologically linked food poisoning clusters affecting tourists of inbound tour groups from the Mainland. The CHP reminded the public to maintain personal, food and environmental hygiene to prevent foodborne diseases.

     All affected persons had meals in Hong Kong and subsequently travelled to Macao with the tour groups and sought medical advice there. According to the epidemiological investigations by the CHP and Macao authorities, two clusters with 13 affected persons were identified.

     The first cluster involved five males and three females aged between 48 and 68, who developed abdominal pain, nausea, vomiting and diarrhoea about 14 hours after having lunch in a restaurant in To Kwa Wan on July 22. The stool sample of one of the patients tested positive for Vibrio parahaemolyticus.

     The other cluster involved two males and three females aged between 26 and 57, who developed similar symptoms about nine hours after having lunch in the same restaurant on the same day.

     All affected persons have sought medical advice in Macao. None of them required hospitalisation and all of them are in stable condition.

     Initial investigations revealed that the affected persons aforementioned had consumed common food, while some other tour groups also patronised the restaurant. The CHP is currently contacting other tour groups to find out if they are being affected. The personnel from the Centre for Food Safety (CFS) of the Food and Environmental Hygiene Department have conducted an inspection at the restaurant. The investigations of the CHP and the CFS are ongoing.