Category Archives: STEC E.coli

RASFF Alert – STEC E.coli – Minced Beef

RASFF-Logo

RASFF – shigatoxin-producing Escherichia coli (stx+ eae+ /25g) in minced beef from Belgium in Belgium

 

USA – Consumers urged to check homes for beef implicated in illness – STEC E.coli

Food Safety News

Ecoli Istock

Image CDC

Illinois officials have issued a public alert regarding certain ground beef because product testing following a reported illnesses showed E. coli contamination.

Testing showed the non-O157 E. coli was present in beef prepared by a “custom exempt” establishment, David B’s Custom Meats in Carlinville, IL, in Macoupin County, according to a notice from the Illinois Department of Agriculture (IDOA). 

There is concern that consumers may still have unused portions of the implicated beef in their homes. The ground beef has been sold since the beginning if this year and was still on sale as of yesterday. Freezing does not kill E. coli bacteria, so consumers are urged to check their freezers and refrigerators for the implicated beef.

USA – David B’s Custom Meats recalls ground beef after E. coli illness and positive test – STEC E.coli

Food Poison Journal

The Illinois Department of Agriculture (IDOA) is issuing a public health alert due to an undetermined amount of ground beef product prepared under custom exemption that may be contaminated with Non-O157 Shiga toxin E. coli (STEC), a bacteria commonly known as “E. coli”. That product was prepared at a Type II Establishment (custom exempt), David B’s Custom Meats, located in Carlinville Illinois, in Macoupin County at the beginning of 2020 to current. Custom exempt meat products are not inspected and cannot be offered for sale; because of this, a recall of the affected product was not requested.

The problem was discovered when a resident of Macoupin County notified local public health officials about sickness after consuming ground beef. The sample collected from the remaining product tested positive for presence of NON-O157 Shiga toxin-producing E.coli.

USA – 43 Thousand Pounds of Hamburger recalled over E. coli O157

Food Poison Journal

Lakeside Refrigerated Services, a Swedesboro, N.J. establishment, is recalling approximately 42,922 pounds of ground beef products that may be contaminated with E. coli O157:H7, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.

The raw ground beef items were produced on June 1, 2020.

Iceland – Monitoring of Sunu 2019

Monitoring of Sunu 2019

Salmonella increased in poultry and pigs in 2019 compared to the year before there was an increase in salmonella infections in humans. The rapid increase in the illness caused by toxin producing E. coli (STEC) in people when the cycle of infection occurred last summer in the south . Food has published a report on the monitoring agents exhibit and antibiotic resistance in 2019 on the website of the Agency.

Sununu are diseases or pathogens (súnuvaldar) transmitted between humans and animals. Control of salmonella (Salmonella spp.) And Campylobacter (Campylobacter spp.) In the first stages of the food chain is powerful in the country and deliver increased consumer food safety. Other food-borne bacteria cause disease in people less than in the light of the very serious illnesses may include monitoring of the bacteria no less important.

The incidence of salmonella in poultry, the products of poultry and pigs increased from the previous year. The increase was due to repeated infected a few farms where it has been difficult to get rid of certain strains of bacteria. A similar increase was not found in people and it indicates that the monitoring of fire and slaughterhouses, in accordance with national programs, minimize the risk of contamination of poultry and pork in people. Whole Gene Research conducted in support that conclusion. While salmonella was found in one sample from the domestic pork market reminiscent of the constant need to be alert and to encourage proper treatment of food with consumers.

No significant changes were observed in the prevalence of Campylobacter in people or poultry and their products. Campylobacter was detected in three samples of frozen chicken meat market (2.1% of samples), samples from both domestic and foreign meat. In all cases, this was a very little bacterial contamination or below the detection limit (<10 cfu / g) counting method which dies Campylobacter mostly frozen.

The rapid increases in illness in people due to infections caused by toxin producing E. coli (STEC), which is explained primarily by a rally in the summer of 2019 in which 22 children and two adults became ill. So far, the incidence of this infection have been very low in people in this country, one to three cases a year. Epidemiological evidence regarding the cycle and screening conducted in 2018 and 2019 for STEC in meat market suggest that the bacteria are part of the microflora of Icelandic cattle and sheep. Research is needed, however, better prevalence of STEC in livestock and focus on preventive measures in slaughterhouses and meat processors to reduce the risk of spreading STEC in meat. Moreover, it must avoid the unclean artifacts present at the slaughterhouse.

Listeria (Listeria monocytogenes) found regularly in the known risks and production environment. That year the initiatives contained in the monitoring of food businesses that produce food ready for consumption, with an emphasis on smoke and buried fishery products, cheeses and meats. Go particular the sampling plan companies and their preventive measures against listeria.

We import control of animal products from third countries are regularly sampled for analysis of Salmonella, Listeria (L. monocytogenes) and E. coli. In 2019 were sampled consignments of eggs, cooked shrimp, mysudufti, meat products (prepared chicken dishes) and pet food. The samples were all negative.

Click to access sunur-syklalyfjaonaemi-2019.pdf

USA – FDA Releases Report on Investigation into Fall 2019 Outbreaks of Illnesses Tied to Romaine Lettuce

FDA

 

As the nation grapples with the COVID-19 public health emergency, the U.S. Food and Drug Administration is grateful for all that the food and agricultural sector is doing to provide safe and available food to consumers during this difficult time. As we work to get through the current challenge together, the FDA remains committed to protecting both the safety of workers and consumers from foodborne illness as we strive to ensure that America’s food supply remains resilient and among the safest in the world. As part of our ongoing efforts to combat foodborne illness, the FDA released the findings of an investigation into three outbreaks of E. coli O157:H7 illnesses that occurred in Fall 2019, all tied to romaine lettuce, that suggests the proximity of cattle to produce fields may have been a contributing factor.

Investigational Findings

The FDA worked with the Centers for Disease Control and Prevention (CDC) and state partners to investigate the contamination of romaine lettuce with several strains of E. coli O157:H7 that caused three outbreaks of foodborne illness beginning in September 2019 and which were declared over in January 2020. Some clusters (but not all) within each of these outbreaks were traced back to a common grower with multiple ranches/fields located in the Salinas, CA, growing region. Together, the outbreaks made 188 people ill.

During the course of on-farm investigations, one of the outbreak strains of E. coli O157:H7 was detected in a sample on public land less than two miles upslope from a produce farm with multiple fields that were identified during the traceback investigations. Other Shiga toxin-producing strains of E. coli (STEC) were found in closer proximity to where romaine lettuce crops were grown, including two samples from the border area of a farm immediately next to cattle grazing land in the hills above leafy greens fields and two samples from on-farm water drainage basins.

While these strains were not tied to the outbreaks, they do offer insight into the survival and movement of pathogens in this growing region. These findings, together with the findings from earlier leafy greens outbreaks, suggest that a potential contributing factor has been the proximity of cattle to the produce fields identified in traceback investigations. This is especially true when cattle are adjacent to and at higher elevations than produce fields.

In the report, “Investigation Report: Factors Potentially Contributing to the Contamination of Romaine Lettuce Implicated in the Three Outbreaks of E. coli O157:H7 During the Fall of 2019,” we’re calling on leafy greens growers to assess and mitigate risks associated with adjacent and nearby land uses, including grazing lands and animal operations. Of note, the number of cattle observed on nearby lands during the 2019 investigations was far lower than the volume of a large Concentrated Animal Feeding Operation, offering a useful reminder that high-density animal operations are not the only factor to consider. These key findings reinforce our concern about the possible impact of nearby and adjacent land use on the safety of leafy green crops and further underscore the importance of reviewing current operations and implementing appropriate risk mitigation strategies.

Research – Antibacterial activity and mechanism of Litsea cubeba essential oil against food contamination by Escherichia coli and Salmonella enterica

Wiley Online

Litsea cubeba essential oil (LC‐EO) has been reported as antibacterial agents, but there are few studies about its possible antibacterial mechanism. The antibacterial activities and the underlying mechanisms of LC‐EO against Escherichia coli O157: H7 and Salmonella enterica were investigated. The results showed that the LC‐EO was more effective against gram‐negative bacteria. The inhibition zone for E. coli O157: H7 and S. enterica were 3.1 ± 0.8 and 4.5 ± 0.6 mm, respectively. The minimum inhibitory concentration of LC‐EO against both bacteria was 0.9 μg/ml, while the minimum bactericidal concentrations were 4.5 and 9 μg/ml. Gas chromatography–mass spectrometry analysis confirmed that citral (86.8%) was the main component of LC‐EO. The results of a time–kill analysis illustrated that treatment with LC‐EO led to a rapid decrease in viable bacterial cell number. The release of electrolytes and nucleic acids from the bacterial cells increased with the dose of LC‐EO. Propidium iodide uptake revealed that LC‐EO caused cell membrane damage. Scanning electron and transmission electron microscopy showed that LC‐EO caused damage to the cell walls and membranes, resulting in cell deformation, atrophy, and large central voids. Thus, LC‐EO may provide the basis for the development of new natural food preservatives.

Research – Effectiveness and Functional Mechanism of a Multicomponent Sanitizer against Biofilms Formed by Escherichia coli O157:H7 and Five Salmonella Serotypes Prevalent in the Meat Industry

Journal of Food Protection

ABSTRACT

Biofilm formation by Escherichia coli O157:H7 and Salmonella enterica at meat processing plants poses a potential risk of meat product contamination. Many common sanitizers are unable to completely eradicate biofilms formed by these foodborne pathogens because of the three-dimensional biofilm structure and the presence of bacterial extracellular polymeric substances (EPSs). A novel multifaceted approach combining multiple chemical reagents with various functional mechanisms was used to enhance the effectiveness of biofilm control. We tested a multicomponent sanitizer consisting of a quaternary ammonium compound (QAC), hydrogen peroxide, and the accelerator diacetin for its effectiveness in inactivating and removing Escherichia coli O157:H7 and Salmonella enterica biofilms under meat processing conditions. E. coli O157:H7 and Salmonella biofilms on common contact surfaces were treated with 10, 20, or 100% concentrations of the multicomponent sanitizer solution for 10 min, 1 h, or 6 h, and log reductions in biofilm mass were measured. Scanning electron microscopy (SEM) was used to directly observe the effect of sanitizer treatment on biofilm removal and bacterial morphology. After treatment with the multicomponent sanitizer, viable E. coli O157:H7 and Salmonella biofilm cells were below the limit of detection, and the prevalence of both pathogens was low. After treatment with a QAC-based control sanitizer, surviving bacterial cells were countable, and pathogen prevalence was higher. SEM analysis of water-treated control samples revealed the three-dimensional biofilm structure with a strong EPS matrix connecting bacteria and the contact surface. Treatment with 20% multicomponent sanitizer for 10 min significantly reduced biofilm mass and weakened the EPS connection. The majority of the bacterial cells had altered morphology and compromised membrane integrity. Treatment with 100% multicomponent sanitizer for 10 min dissolved the EPS matrix, and no intact biofilm structure was observed; instead, scattered clusters of bacterial aggregates were detected, indicating the loss of cell viability and biofilm removal. These results indicate that the multicomponent sanitizer is effective, even after short exposure with dilute concentrations, against E. coli O157:H7 and S. enterica biofilms.

HIGHLIGHTS
  • No viable biofilm cells were detected after treatment with the multicomponent sanitizer.
  • Prevalence of both pathogens was low after treatment with the multicomponent sanitizer.
  • SEM analysis revealed that treatment dissolved the EPS matrix and destroyed the biofilm.

Research – Inhibitory effect of thyme and cinnamon essential oils against E. coli O157:H7 in Tahini

Scielo

Tahini is a common food product in the Mediterranean area that is used as a main ingredient in variety of ready-to-eat foods. The objective of the current study was to investigate the inhibitory effect of thyme oil (TO) or cinnamon oil (CO) on E. coli O157:H7 viability in tahini and diluted tahini at different storage temperatures. Addition of 2.0% CO to tahini reduced E. coli O157:H7 numbers by 1.38, 1.79 or 2.20 log10 CFU/mL at 10, 25 or 37 °C, respectively, by 28d. In diluted tahini at 10 °C, no viable cells of E. coli O157:H7 by 21d were detected when 1.0% CO was used. However, at 25 and 37 °C, no viable cells were detected by 14d when CO was added at 0.5% level. Addition of 2.0% TO to tahini, resulted in 1.82, 2.01 or 1.65 log10 CFU/mL reduction in E. coli O 157:H7 numbers was noted at 37, 25 or 10 °C, respectively, by 28d. In diluted tahini, TO at 0.5% or 1.0% induced complete reduction in the viability of E. coli O157:H7 by 28d storage at 37 or 25 °C. At 10 °C, a 3.02 log10 CFU/mL reduction was observed by 28d compared to the initial inoculation level in samples treated with 2.0% TO.

USA – Ravioli with E. coli recalled

Food Poison Journal

The U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) is issuing a public health alert because approximately 70 pounds of raw beef ravioli products, produced by P&S Ravioli Company, a Philadelphia, Pa. establishment, may be contaminated with E. coli O157:H7. A recall was not requested because the affected product is no longer available for purchase.