Category Archives: Mould Toxin

Research – Comparative study of microbiological transfer from four materials used in direct contact with apples

Science Direct

Several materials such as plastic, wood, cardboard or stainless steel are used as working surfaces or packaging in direct contact with foodstuffs. In food industries, the hygienic surface status is one of the criteria to product conform packaging as described in the European regulation ECR 1935/2004. Today in European Union, it exists one harmonized regulation specific for Food Contact material made of plastic called EU N°10/2011 (Anonymous 2011a). This regulation specifies that materials intended for safe foodstuff contact must not modify food characteristics in terms of chemical, microbiological and sensorial properties.

This study aims to compare the survival and transfer of Penicillium expansum conidia and Escherichia coli cells from several materials to apples. Poplar, cardboards, newly manufactured plastic and reusable plastic specimens were artificially inoculated with both microorganisms, subsequently put in contact with apples and stored under realistic storage conditions. After incubation for up to 1 week, apples and specimens were analysed to assess the survival of the microorganisms and their transfer from materials to apples.

While P. expansum survived and did not grow on any of the materials, E. coli mortality was observed after 1 h on wood and cardboard and after 1 week on both plastics. The proportion of microorganisms transferred was different according to the considered material. This transfer was lower than 1% for wood.

Research – The effect of royal jelly and propolis alone and in combination on inhibition of Aspergillus parasiticus growth, aflatoxin production, and aflR gene expression

Wiley Online

The objective of this study was to determine the inhibitory effect of royal jelly (RJ) and propolis on growth, aflatoxin production and aflR gene expression in Aspergillus parasiticus . Inhibitory effect of RJ and propolis against a standard strain of A. parasiticus (ATCC 15517) was determined alone and in combination in accordance with the CLSI M38‐A2 and checkerboard methods, respectively. The aflatoxin concentrations in the control and treated media were determined by HPLC. Also, the quantitative changes in the aflR gene expression were analyzed. The minimum inhibitory concentrations (MIC) of RJ and propolis alone were 3,200 and 100μg/ml, respectively. Also, the MICs of RJ and propolis in combination were 200 and 25μg/ml, respectively. When combined, a synergistic interaction was observed with a FICI of 0.312. Total levels of aflatoxin decreased from 386.1ppm to 8.72, 3.01 and 1.75ppm at 1,600μg/ml of RJ, 50μg/ml of propolis and 100+12.5μg/ml of RJ and propolis, respectively. In addition, the level of afIR gene expression was significantly decreased after treatment with RJ and propolis extracts alone and with their combination. The findings reveal that RJ and propolis extracts, either alone or in combination, have a significant inhibitory effect on aflR gene expression in aflatoxin production.

Research – Quantitative microbial spoilage risk assessment (QMSRA) of pasteurized strawberry purees by Aspergillus fischeri (teleomorph Neosartorya fischeri)

Science Direct

Aspergillus fischeri ascospores are known as potential spoilage microorganisms of pasteurized fruit products due to their high incidence in fruits, the ability to survive pasteurization and to grow in acidic conditions. This study aimed to develop a quantitative microbial spoilage risk assessment (QMSRA) model approach to estimate the spoilage risk of packaged strawberry purees due to A. fischeri under various scenarios regarding product formulation, processing and storage conditions. The development of the risk assessment comprised three steps: (1) initial contamination level of raw material by ascospores (N0), (2) inactivation of ascospores during thermal processing (Np) and (3) determination of the number of ascospores which are able to survive thermal processing and develop visible mycelia (D = 2 mm) during storage (Nf). Data of visible growth (tv, days) comprised distributions previously obtained as function of water activity (aw) (0.860–0.985), oxygen (0–21%), temperature (8–30 °C) and pasteurization (95–105 °C/15 s). The simulations were performed in triplicate with 100,000 iterations using the software R. The outcome “spoilage risk” was defined as the probability of having at least one ascospore (Nf) capable of forming visible colonies in 100 g-pack strawberry puree within the typical use-by dates. Overall, high probabilities of spoilage were estimated for purees pasteurized at milder treatments at 85 °C/15–60 s (67%) and 90 °C/15–60 s (≥40%) stored at ambient temperature (22 °C). The spoilage risk was only effectively reduced (0.02%) by increasing pasteurization conditions to 95 °C for at least 45 s. Moreover, the microbial stability of such purees, i.e., spoilage risk <0.001% (=less than 1 spoilage pack out of 105 produced units) was predicted to occur for purees treated at 100 °C/15 s or stored at chilled conditions (≤8 °C) or at strict anaerobic conditions or produced as concentrates (aw ≤ 0.860). Based on the outcomes obtained, a set of specifications for Heat-Resistant Moulds (HRMs) in raw material and pasteurized purees aimed to be used as an ingredient was suggested. Furthermore, the results can be used to support risk management decisions in identifying and quantifying the impact of possible interventions during formulation, processing and storage conditions of fruit purees to effectively reduce this risk.

RASFF Alerts – Aflatoxin – Dried Figs – Chakki Atta Wheat Flour

European Food Alerts

RASFF

Aflatoxins (B1 = 10.5; Tot. = 25.1 µg/kg – ppb) in dried figs from Turkey in Belgium

RASFF

Aflatoxins (B1 = 7.5; Tot. = 8.2 µg/kg – ppb) in chakki atta wheat flour from Nepal, via India in the UK

RASFF Alert- Aflatoxin – Candies with Peanuts

RASFF-Logo

RASFF – aflatoxins (B1 = 3.57; Tot. = 4.10 µg/kg – ppb) in candies with peanuts from Ukraine, with raw material from India in Poland

RASFF Alert – Mycotxin – Fumonsins – Corn Flour

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RASFF – fumonisins (18255 µg/kg – ppb) in corn flour from Peru in Spain

RASFF Alert – Animal Feed – Aflatoxin – Shelled Groundnuts

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RASFF -aflatoxins (B1 = 74 µg/kg – ppb) in shelled groundnuts from Argentina in the UK

Research – Effects of smoke produced from smoldering plants on the Aspergillus flavus growth and production of aflatoxin in pistachio

Wiley Online

Aflatoxin (AF) contamination of the Iranian exporting pistachio has become a major problem in the last decades. In this study, the antifungal effects of smoke produced from the smoldering of several herbal plants were investigated. Four different ratios of plant weight/exposure time (5/5, 10/15, 15/30, and 20 g/45 min) were used from each plant material to smoke two isolates of Aspergillus flavus (A47 and A3), grown in potato dextrose agar (PDA). The results showed that the 20 g/45 min treatment using smoldering cinnamon bark, neem leaves, and clove flowers had efficient inhibitions of 100, 85, and 75%, respectively. Furthermore, the smoking of pistachio inoculated with a spore concentration of 1 × 106 / ml using 30 g of cinnamon bark, neem leaves, and clove flowers for 75 min was capable of preventing the production of different types of AF, that is, B1, B2, G1, and G2 in the treated products. There is a good potential to smoke pistachio with these three herbal plants and prevent the production of AF in pistachio during the handling, storage, and transportation.

Tanzania -Caution Cited On Aflatoxin Threat to Food Security

All Africa

INCREASING contamination of food crops with aflatoxin is, besides jeopardising public health, threatening the country’s food security, experts have warned, proposing proper handling of food from farming to storage phases.

“Aflatoxin contamination is a serious problem that demands concerted mitigation efforts,”Tanzania Initiatives for Preventing Aflatoxin Contamination (TANIPAC) Project Officer Ali Hamad said yesterday.

Speaking at the ongoing third Nanenane exhibitions at Chamanangwe grounds here, the officer blamed aflatoxin on improper farming, harvesting, processing and storage of food crops.

Kenya – Kebs raises concern on high levels of aflatoxin in maize

Daily Nation 

The Kenya Bureau of Standards (Kebs) and consumers have raised concern on high levels of aflatoxin in maize, the staple food for most Kenyan households.

The complaints come even as processors experience acute shortage of the produce.

The millers say their stocks were exhausted two months to the harvest of this season’s crop in the North Rift, the country’s grain basket.

Many millers in western Kenya are getting maize from schools and other learning institutions that were closed in March to stop the spread of coronavirus.