Probiotics in fermented foods or commercially available supplements benefit the host by providing metabolites/peptides. The production of these metabolites varies with available substrates/prebiotic present in the system and their concentration. In this study, 0.5% peanut flour (PF) was used to stimulate the growth and production of metabolites of wild-type Lactobacillus casei (LC wt ) and compare with an engineered L. casei (LC CLA ) capable of converting a higher amount of conjugated linoleic acid (CLA). The total extracellular metabolites present in the cell-free cultural supernatant (CFCS) of LC wt (without peanut), LC wt+PF (with peanut), and LC CLA were collected after 24 h and 48 h of incubation, and their antagonistic activities against enterohemorrhagic Escherichia coli (EHEC EDL933) growth and pathogenesis were evaluated. All the collected metabolites exhibited varying efficiency in restraining EDL933 growth while supplementing low concentration of CLA to the 48-h CFCS from LC wt showed augmented antagonism toward EDL933. A downregulation of key virulence genes was observed from metabolites collected at 48-h time point. These observations indicate that the presence of metabolites in CFCSs including CLA, produced by Lactobacillus , which was further identified by gas chromatography-mass spectrometry; plays a critical role. This study demonstrates the potential applicability of Lactobacillus -originated CLA in the prevention of EDL933 mediated illnesses.
NELLORE: At least 15 children fell sick after they reportedly ate adulterated food at an Anganwadi in Podalakuru town of Nellore district. According to reports, the children were suffering from diarrhoea since Wednesday evening. Parents staged a demonstration at the Anganwadi and blamed the negligent attitude of the staff for delaying treatment to their children.
They alleged that the children began vomiting and fell sick after eating food provided at the Anganwadi. They said the children were admitted to a local hospital for treatment where doctors confirmed that they fell sick due to the adulterated food they ate. The children are getting treated at various hospitals in Podalakuru and Nellore city. Sources said their condition is stable now.






