Category Archives: Food Microbiology Research

France – Nestlé allowed to restart pizza plant linked to deadly E. coli outbreak

Food Safety News

Nestlé has been given permission to partially reopen a plant in France where pizzas behind a serious E. coli outbreak were made.

French officials from the Nord area of the country in the Hauts-de-France region gave the green light this past week for operations to restart at the Buitoni factory in Caudry.

Approval only applies to production of pizzas with cooked dough. The line making pizzas with no re-baking of dough, such as the Fraîch’Up range, will not restart yet.

Fraîch’Up frozen pizzas were the source of the largest E. coli-HUS outbreak ever documented in France. 

One element of the plan to restart has included the dismantling and cleaning of 19,000 parts on the production line.

England – Differential impact of the COVID-19 pandemic on laboratory reporting of Norovirus and Campylobacter in England: A modelling approach

PLOS One

CDC Campy

The COVID-19 pandemic has impacted surveillance activities for multiple pathogens. Since March 2020, there was a decline in the number of reports of norovirus and Campylobacter recorded by England’s national laboratory surveillance system. The aim is to estimate and compare the impact of the COVID-19 pandemic on norovirus and Campylobacter surveillance data in England.

USA – FDA Advises Restaurants and Retailers Not to Serve or Sell and Consumers Not to Eat Product Labeled as Sun Hong Foods, Inc. Enoki Mushrooms Sourced from China Due to Possible Listeria Contamination

FDA

December 17, 2022

Audience

  • Restaurants and food retailers that have received Sun Hong Foods Inc. enoki mushrooms sourced from China.
  • Consumers, especially those who are or could become pregnant, the elderly, and persons with weakened immune systems, who have recently consumed enoki mushrooms labeled as Sun Hong Food, Inc. product sourced from China.

Product

  • Product is labeled as Sun Hong Foods Inc, (Montebello, CA) enoki mushrooms sourced from China.

Purpose

  • The U.S. Food and Drug Administration is advising consumers not to eat, restaurants and food retailers not to sell, and to dispose of product labeled as Sun Hong Foods Inc. enoki mushrooms sourced from China due to possible contamination with Listeria monocytogenes.

Summary of Problem and Scope

  • On November 28, 2022, Missouri state partners, as a part of the FDA-funded Laboratory Flexible Funding Model Cooperative Agreement Program collected enoki mushroom samples from retail markets in Missouri. One sample labeled as Sun Hong Foods Inc, (Montebello, CA) and sourced from China tested positive for Listeria monocytogenes, but does not appear to match any clinical isolates from active outbreaks.

FDA Actions

  • The FDA is issuing this alert advising consumers to not eat and restaurants and food retailers to not sell Sun Hong Foods Inc. enoki mushrooms sourced from China due to possible Listeria monocytogenes contamination. FDA is also advising consumers, restaurants and food retailers to dispose of Sun Hong Foods Inc. enoki mushrooms sourced from China.
  • The FDA is awaiting information on further interstate distribution of the enoki mushrooms and will continue to monitor the investigation and provide assistance to state authorities as needed.

Symptoms of Listeriosis

There are a range of symptoms for listeriosis. Depending on the severity of the illness, symptoms may last from days to several weeks. Mild symptoms may include a fever, muscle aches, nausea, vomiting, and diarrhea. If the more severe form of listeriosis develops, symptoms may include headache, stiff neck, confusion, loss of balance, and convulsions. For the very young, the elderly, and the immune-compromised listeriosis can result in death.

People infected with L. monocytogenes may start to see symptoms in a few hours or as long as two to three days after eating contaminated food. More severe forms of listeriosis may take anywhere from three days to three months to develop.

Listeriosis can be particularly dangerous for pregnant women and their newborn babies, leading to serious complications with their pregnancy, including miscarriage and stillbirth. Babies born with a listeriosis infection may develop severe health complications that require immediate medical attention, lead to lifelong health problems, or result in death. Women who suspect they have symptoms of listeriosis (muscles aches, nausea, stiffness in neck, headaches, etc.) should seek medical care immediately and tell their health provider what they ate. Learn more about People at Risk of Foodborne Illness.

Due to the range in severity of illness, people should consult their health care provider if they suspect that they have developed symptoms that resemble a(n) L. monocytogenes infection.

Additional Information 

Research – Life and death of an ‘altruistic’ bacterium

Science Daily

Biofilms, complex communities of bacteria, abound around us: on the surface of cheese where they give off flavors and aromas, in streams where they form the slimy substance on rocks, on our teeth where they form plaque.

Living in a biofilm provides numerous advantages to bacteria: things like resource sharing, shelter from predators, and increased resistance to toxic compounds such as antibiotics.

But having the option to leave the biofilm when environmental conditions deteriorate can be a plus for bacteria, too, allowing them to relocate to a more hospitable environment.

”For the bacterium Caulobacter crescentus, the biofilm becomes a kind of prison in perpetuity: once cells are attached to a surface through a strong adhesive at one end of the cell, they cannot leave the biofilm,” said Yves Brun, a professor in the Department of Microbiology, Infectious Diseases and Immunology at Université de Montréal.

”However, when these attached cells divide, their unattached ‘daughter’ cells have a choice of joining the biofilm or swimming away.”

Research -Microbial safety and sanitary quality of strawberry primary production in Belgium: risk factors for Salmonella and Shiga toxin producing Escherichia coli (STEC) contamination.

PDF

ABSTRACT

Strawberries are an important fruit in Belgium both in production and consumption, but little information is available about the presence of Salmonella and STEC in these berries, the risk factors in agricultural production and possible specific mitigation options.

In 2012, a survey was undertaken of three soil and three soilless cultivation systems in Belgium.

No Salmonella spp. was isolated. No STEC was detected in the strawberry samples (0 out of 72), but STEC was detected by qPCR in 11 out of 78 irrigation water and 2 out of 24 substrate samples.

Culture isolates were obtained for 2 out of 11 qPCR positive irrigation water samples and 2 out of 2 substrate samples. Multivariable logistic regression analysis revealed elevated generic E. coli numbers (odds ratio (OR) for 1 log increase being 4.6) as the most important risk factor for STEC, together with the berry picking season (elevated risk in summer).

Presence of generic E. coli in the irrigation water (≥ 1 cfu per 100 ml) was mainly influenced by the type of irrigation water (collected rainfall water stored in ponds was more often contaminated than ground water pumped from boreholes (OR = 5.8)) and the lack of prior treatment (untreated water versus water subjected to sand filtration prior to use (OR = 19.2)).

The follow-up study in 2013 at one of the producers indicated cattle as the most likely source of STEC contamination of the irrigation water.

Research – An outbreak of Cryptosporidium parvum linked to pasteurised milk from a vending machine in England: a descriptive study, March 2021

Cambridge Org

We describe the investigations and management of a Cryptosporidium parvum outbreak of linked to consumption of pasteurised milk from a vending machine. Multiple locus variable number of tandem repeats analysis was newly used, confirming that C. parvum detected in human cases was indistinguishable from that in a calf on the farm. This strengthened the evidence for milk from an on-farm vending machine as the source of the outbreak because of post-pasteurisation contamination. Bacteriological indicators of post-pasteurisation contamination persisted after the initial hygiene improvement notice. We propose that on-farm milk vending machines may represent an emerging public health risk.

Research -Quantitative microbiological risk assessment of traditional food of animal origin produced in short supply chains in Poland

EFSA

Abstract

Polish raw-milk cheeses produced in short supply chains may pose a threat to consumer safety due to pathogen presence. Listeria monocytogenes is a bacterium of great importance for the food safety of refrigerated RTE foods due to its ability to grow at refrigeration temperatures.

During the EU-FORA fellowship, a stochastic risk assessment was designed and executed to estimate the risk for consumers from L. monocytogenes in these products. The aim was to develop a probabilistic QMRA model that would incorporate the variability and uncertainty of the model’s inputs such as prevalence, initial concentration levels, product intrinsic factors, domestic storage temperature and consumer behaviour. The project involved data collection and analysis, growth model selection, mathematical modelling and Monte Carlo analysis in R programming language.

Microbiological and physicochemical testing were carried out throughout the year on two types of cheeses in combination with a domestic refrigerator temperature survey and accompanying consumption questionnaire. Collected data were fitted to probability distributions using R. The appropriate growth model for the pathogen was selected based on an inoculation study performed on one of the raw-milk cheeses and the chosen mathematical model was written into the R script developed for the QMRA. The dose–response model used the ingested dose calculated from the modelled concentration of L. monocytogenes at the time of consumption and the single serving size from the questionnaire to estimate the probability of illness. The final risk was expressed as probability of listeriosis for Polish consumers per serving of raw-milk cheese.

Research – Microbiological Quality of Pig Carcasses in a Slaughterhouse under Risk-Based Inspection System

MDPI

Abstract

Meat product inspection is one of the procedures adopted more than a century ago to guarantee food quality and safety for consumption. Due to technology and regulation advancement for farming and slaughtering pigs, a change in zoonotic profile attributed to pork has been identified.
 
Thus, a global movement began to establish inspection parameters based on epidemiological risk profiles, culminating in the publication of a new regulation in Brazil in 2018. This normative instruction establishes that slaughterhouses under federal inspection must implement risk-based inspection until 2028.
 
Changes in the inspection system can generate questions and objections on the part of customers and consumer markets. In order to assess microbiological contamination when adopting a risk-based inspection system, the occurrence of Salmonella spp. and the quantification of Enterobacteriaceae and mesophilic aerobic counts were compared in pig carcasses slaughtered under traditional and risk-based inspection systems.
 
A statistical significance reduction was identified regarding the quantification of Enterobacteriaceae (log −0.18 to −1.61 CFU/cm2) and mesophilic aerobic counts (log 4.60 to 3.49 CFU/cm2). The occurrence of Salmonella spp. did not show a significant difference (4% to 5.3%). The results allowed us to conclude that adopting risk-based inspection systems improves food safety through Enterobacteriaceae and mesophilic aerobic counts reduction.

Research – Prevalence ofE. coliO157:H7 in water sources: an overview on associated diseases, outbreaks and detection methods

Elsevier

Escherichia coliO157:H7 is a zoonotic pathogen with its ability to cause human illness ranging from diarrheal dis-ease to fatal hemolytic uremic syndrome.E. coliO157:H7 had been associated with waterborne outbreaksresulting in high morbidity and mortality worldwide. Therefore, it is important to investigate the prevalence ofE. coliO157:H7 in water sources especially used for drinking and to develop the diagnostic methods for itsearly detection. The review describes traditional cultural methods, immunological techniques, and polymerasechain reaction (PCR)–based methods for detection of this bacterium in water sources. The current PCR-basedtechniques such as real-time PCR are more specific and sensitive and require less detection time (b3 hours).These methods can be applied for regular water monitoring and proper management of water sources to preventwaterborne diseases due toE. coliO157:H7.

 

Research – Vibrio vulnificus and Vibrio parahaemolyticus in Oysters under Low Tidal Range Conditions: Is Seawater Analysis Useful for Risk Assessment?

MDPI

Food Illness

Abstract

Human-pathogenic Vibrio bacteria are acquired by oysters through filtering seawater, however, the relationships between levels of these bacteria in measured in oysters and overlying waters are inconsistent across regions. The reasons for these discrepancies are unclear hindering our ability to assess if -or when- seawater samples can be used as a proxy for oysters to assess risk. We investigated whether concentrations of total and human pathogenic Vibrio vulnificus (vvhA and pilF genes) and Vibrio parahaemolyticus (tlhtdh and trh genes) measured in seawater reflect concentrations of these bacteria in oysters (Crassostrea virginica) cultured within the US lower Chesapeake Bay region. We measured Vibrio spp. concentrations using an MPN-qPCR approach and analyzed the data using structural equation modeling (SEM). We found seawater concentrations of these bacteria to predictably respond to temperature and salinity over chlorophyll a, pheophytin or turbidity. We also inferred from the SEM results that Vibrio concentrations in seawater strongly predict their respective concentrations in oysters. We hypothesize that such seawater-oyster coupling can be observed in regions of low tidal range. Due to the ease of sampling and processing of seawater samples compared to oyster samples, we suggest that under low tidal range conditions, seawater samples can foster increased spatial and temporal coverage and complement data associated with oyster samples.