Category Archives: Food Microbiology Research

Research – CFA Briefing – Relevance of generic E coli (inc testing issues) to food safety

Chilled Food Assoc

Escherichia coli (E. coli) is a bacterium commonly found in the gut of humans and warm-blooded
animals. Most strains of E. coli are harmless and some provide many health benefits to the host; for example,
they prevent colonization of the gut by harmful pathogens.

However, there are small groups of E.coli, referred to as pathogenic E.coli, that can cause severe disease in
humans, which are well known to be transmitted by food and/or water and have been implicated in major
food borne outbreaks worldwide e.g. radish sprouts in Japan, Sprouted seeds in Germany, raw / undercooked
beef products UK and USA.

This briefing note looks at the relevance of generic E coli to food safety referring to fresh produce and is also relevent to all food types.

With sections including transmisson, testing and prevention it concludes:

  • A positive for E.coli O157 alone may not cause illness.
  • The isolation of stx (Shiga toxins) gene has to be isolate in an E.coli, usually with a gene for attachment for the isolate to cause serious illness.
  • All parties agree with the confirmed stage of the method, but FSA’s decision to call isolation of stx alone as presumptive is misleading.
  • Action required in the event of a presumptive result is inappropriate and not possible for the industry.
  • Food safety can only be controlled by routinely testing for indicator organisms e.g. generic E. coli and monitoring trends to proactively prompt investigation.
  • HACCP and PRP’s are paramount, supported by environmental and product monitoring.

CFA 057 22 Briefing paper – relevance of generic E coli (inc testing issues) to food safety

USA – FDA Warning Letter – Mariscos Bahia, Inc. – Salmonella Outbreak

FDA

The United States Food and Drug Administration (FDA) and the California Department of Public Health (CDPH) inspected your seafood processing facility, Mariscos Bahia Inc, located at 8300 Rex Rd., Pico Rivera, CA 90660-3719 from October 6 to October 21, 2022. The inspection was initiated as part of a multistate foodborne outbreak investigation of Salmonella Litchfield (S. Litchfield) infections linked to seafood, including fresh, raw salmon, supplied by your facility. According to the Centers for Disease Control and Prevention (CDC), 39 people from 4 states were infected with the outbreak strain of S. Litchfield. During our inspection we collected environmental samples (swabs) from various areas in your facility, including areas that are near food during processing operations. FDA laboratory analyses found the presence of the outbreak strain of S. Litchfield and two additional Salmonella strains, Salmonella Mbandaka (S. Mbandaka) and Salmonella Oranienburg (S. Oranienburg), which are human pathogens, in your facility. On October 20, 2022, your firm recalled various types of fresh fish processed at your facility and distributed between June 14, 2022 and October 17, 2022, because they had the potential of being contaminated with Salmonella. FDA and CDC have determined, based upon the epidemiologic, traceback evidence, and whole genome sequencing (WGS) matches between clinical and FDA environmental samples, that fresh salmon processed at your facility was the likely source of this multistate S. Litchfield outbreak.

USA – FDA -Core Investigation Table

FDA

What’s New

  • For the outbreak of Salmonella Hartford (ref # 1144) in a not yet identified product, the case count has increased from 47 to 50 cases. FDA has also initiated an onsite inspection and sample collection and analysis.
Date
Posted
Ref Pathogen
or
Cause of
Illness
Product(s)
Linked to
Illnesses

(if any)
Total
Case
Count

Status
3/8/2023 1144 Salmonella Hartford Not Yet
Identified
50 Active
3/1/2023 1143 Hepatitis A Virus Frozen Organic
Strawberries
See
Outbreak
Advisory
Active
2/15/2023 1123 Listeria
monocytogenes
Not Yet
Identified
See
CDC
Investigation
Notice
Active
11/9/2022 1127 Listeria
monocytogenes
Enoki
Mushrooms
See
Outbreak
Advisory
Active

Research – Proposal and Verification of the Theory of Layer-by-Layer Elimination of Biofilm in Listeria monocytogenes

MDPI

Abstract

Biofilms are microbial communities that represent a high abundance of microbial life forms on Earth. Within biofilms, structural changes during clearance processes occur in three spatial and temporal dimensions; therefore, microscopy and quantitative image analysis are essential in elucidating their function. Here, we present confocal laser scanning microscopy (CLSM) in conjunction with ISA-2 software analysis for the automated and high-throughput quantification, analysis, and visualisation of biofilm interiors and overall biofilm properties in three spatial and temporal dimensions. This paper discusses the removal process of Listeria monocytogenes (LM) biofilms using slightly acidic electrolytic water, non-electrolytic hypochlorite water, and alternating the use of strongly acidic and strongly alkaline electrolytic water. The results show that the biofilm gradually thins and gutters from the initial viscous dense and thick morphology under the action of either biocide. This process is consistent with first-level kinetics. After CLSM filming to observe the biofilm structure, analysis software was used to process and quantify the biovolume, average biofilm thickness, biofilm roughness and other indicators; fluorescence enzyme markers were used to verify the remaining amount of extracellular nucleic acid. In this study, we proposed and validated the theory of layer-by-layer elimination of LM biofilm.

France – GROUND STEAK ON DEMAND – Salmonella

Gov france

Identification information of the recalled product

  • Product category Feed
  • Product subcategory Meats
  • Product brand name WITHOUT
  • Model names or references MEAT SVA
  • Identification of products
    GTIN Date
    109000006386 Use-by date 03/10/2023
  • Packaging GROUND STEAK ON DEMAND
  • Marketing start/end date From 03/10/2023 to 03/10/2023
  • Storage temperature Product to be stored in the refrigerator
  • Further information GROUND STEAK MADE ON DEMAND
  • Geographic area of ​​sale MARL
  • Distributors INTERMARCCHE – CHALONS EN CHAMPAGNE

Practical information regarding the recall

  • Reason for recall PRESENCE OF SALMONELLA IN GROUND STEAK ON DEMAND
  • Risks incurred by the consumer Salmonella spp (causative agent of salmonellosis)

USA – FDA Releases FDA Activities for the Safety of Imported Seafood

FDA

Today, the FDA released Activities for the Safety of Imported Seafood (PDF).  The report shares the comprehensive approach the FDA is taking to ensure that imported seafood consumed in the U.S. meets food safety requirements and the standards of domestically produced seafood.

Seafood is one of the most highly traded food commodities in the world with 2018 total imports accounting for approximately 94% of seafood sold by volume in the U.S. Seafood contains high quality protein and other essential nutrients and is an important part of a healthy diet. The safety of imported seafood, particularly shrimp, the most consumed type of seafood in the U.S., has garnered the attention of Congress and industry, among other stakeholders.

The FDA Strategy for the Safety of Imported Food (Import Strategy) is the roadmap for this report.  It describes a comprehensive approach to imported food safety – guided by four goals:

  • Food Offered for Import Meets U.S. Food Safety Requirements
  • FDA Border Surveillance Prevents Entry of Unsafe Foods
  • Rapid and Effective Response to Unsafe Imported Food
  • Effective and Efficient Food Import Program

The Activities for the Safety of Imported Seafood details how established FDA regulation and innovative programs and technology are employed to support each of the four goals as they relate to imported seafood safety. These include proactively engaging and establishing partnerships with FDA regulatory counterparts in countries that export seafood to the United States; exploring the use of Artificial Intelligence (AI), specifically Machine Learning (ML), to strengthen predictive analytics; and developing new tools that leverage technology such as geographic information system (GIS) to provide spatial intelligence about potential seafood hazards.

EU – Oysters linked to 170 illnesses in Finland; people sick in other countries – Norovirus

Food Safety News

french oysters

Contaminated shellfish are continuing to cause illnesses in several European countries and Hong Kong.

At least 170 people have fallen ill in Helsinki, Finland, after eating oysters in various restaurants in February and March. Cases have also been reported in other Finnish cities.

Norovirus has been found in nine different oyster batches with two from the Netherlands, six from France, and one from Ireland. Importers have withdrawn affected batches from the market.

Officials have tested food from restaurants and taken patient samples, finding norovirus. Some of those sick reported eating oysters.

A report on the outbreak is being prepared for the Finnish Food Authority (Ruokavirasto) and the Finnish Institute for Health and Welfare (THL).

Norovirus is the most commonly identified cause of foodborne outbreaks in Finland. Between 2017 and 2021, oysters caused 11 norovirus outbreaks in which more than 110 people fell ill.

Finland has published four Rapid Alert System for Food and Feed (RASFF) alerts on food poisoning due to norovirus in oysters since late February.

Research – Aged meat not riskier than fresh meat

EFSA

Aged meat does not pose any additional risks compared to fresh meat if it is aged under controlled conditions, EFSA experts concluded in a scientific opinion released today.

Meat ageing is a process during which microbes and enzymes act upon the meat to break down the connective tissue, thereby tenderising the meat and giving it a richer flavour. This can be done through two main methods: wet ageing and dry ageing. Wet ageing is used for beef, pork and lamb that is stored and refrigerated in a vacuum package, while dry aged beef is refrigerated without packaging which results in a dry surface that is cut off before preparation.

“Aged meat has risen in popularity in recent years among the food industry and restaurants yet until now there has been a lack of knowledge about its safety. EFSA’s advice contributes to fill that gap and provides a solid scientific basis for food business operators to produce aged meat that is safe,” said the Chair of EFSA’s Panel on Biological Hazards, Prof Kostas Koutsoumanis.

There are no additional risks involved provided that the specific combination of time and temperature identified in the scientific opinion are observed during the ageing process, said EFSA’s experts. For example, dry aged beef can be considered as safe as fresh beef if ageing is done for up to 35 days at a temperature of 3°C or lower.

USA – Resources for the Control of Listeria monocytogenes for Manufacturers of Soft Fresh Queso Fresco-type CheesesUSA –

FDA

Summary of FDA’s Strategy to Help Prevent Listeriosis Outbreaks Associated with Soft Fresh Queso Fresco-Type Cheeses

This fact sheet is intended to help Queso Fresco-type soft cheese manufacturing groups further their understanding of possible food safety risks that can occur in production and the resources available to help them consistently produce safe food.

What are soft Queso Fresco-type cheeses?

Soft Queso Fresco-type (QFT) cheeses are fresh, unripe cheeses that do not go through a significant aging process. These types of cheeses are white/off-white in color and very moist. They also have a low salt and acid content, which gives them a shorter shelf life compared to that of other aged cheeses. This also means soft QFT cheeses must always be refrigerated. Depending on the country of origin, some of the more popular fresh, soft QFT cheeses are labeled as either Latin-style or Mexican-style cheeses. Well-known examples of QFT cheeses include Oaxaca, Asadero, Queso Blanco, Queso Fresco, Requeson, and Panela, amongst others [1],[2].

What are some concerns with soft Queso Fresco-type cheeses?

The pathogen of primary concern among soft QFT cheeses is Listeria monocytogenes (L. monocytogenes), a type of disease-causing bacteria that specifically causes the infection listeriosis. Outbreaks of listeriosis have been linked with soft QFT cheeses made from raw milk or those that contain ingredients that were contaminated somewhere during the cheese-making process.  Historical outbreaks of listeriosis have been associated with QFT made from unpasteurized milk, but recent outbreaks have been caused by cheeses made from pasteurized milk that was contaminated during the cheese-making process. The soft QFT cheeses linked to past outbreaks were often manufactured by smaller-scale producers. These outbreaks have often affected a higher proportion of pregnant Hispanic women and their newborns. Listeriosis infections in pregnant women can result in serious illness, miscarriage, or even stillbirth. Older adults, newborns, and people with weakened immune systems are also at higher risk for developing serious symptoms associated with listeriosis. In addition to the risk of listeriosis linked to soft QFT cheese, the Centers for Disease Control and Prevention has reported multiple outbreaks linked to other pathogenic bacteria in these products, including SalmonellaCampylobacter jejuni, and Brucella [3],[4].

USA – Raw milk bill requires Hawaii Legislature to choose between health risks or food security

Food Safety News

Hawaii House Bill 521 reads like another attempt to weaken the regulation of raw milk until you notice that the year 3000 is currently listed as the effective date. That might be a long wait for those who want to legalize raw milk and raw milk products in the Aloha State including the bill’s powerful author.

Nevertheless, HB521 is getting serious consideration from Hawaii’s legislative committee. The bill has already been heard by the House Finance Committee and the Committee on Consumer Protection and Commerce. Those two committees agree that the purpose of HB 521 is to:

1) Authorize and decriminalize the sale of raw milk and raw milk products directly to consumers for human consumption, subject to certain conditions; and

(2) Authorize the sale of raw goat milk for pet consumption, subject to certain conditions.