Category Archives: HT-2

Research – Mycotoxin control in cereals: safe guarding food

Safe Food Net

The aim of this research was to review (and where possible improve/refine
current rapid screening methods for the mycotoxin T-2 and its metabolites in
cereals for human consumption.
Researchers engaged with the relevant sector of the cereal processing industry in
Ireland and the UK, and the relevant primary production sector, to gain detailed,
valuable information.
The research conducted was designed to clarify deficits in T-2 toxin control in the
relevant processing and primary production sectors, evaluate the robustness of
current analytical detection and control methods therein, augment same if deemed
necessary, and implement workable T-2 toxin control solutions at sectoral level.
Objectives
The specific objectives of the research project were to:
• Conduct a literature review of the most up-to-date scientific and
regulatory data for T-2 toxin and its metabolites, the specific environmental conditions required for their production, and the impact of climate change on their occurrence and distribution.
• Undertake a thorough analysis of the currently available commercial rapid analytical techniques for the screening of T-2 and HT-2 toxins in cereals for direct human consumption.
• Undertake a survey of the relevant processing and primary production sectors to determine what testing they currently conduct for mycotoxins, to fully understand the shortfalls in relation to mycotoxin monitoring and control within the industry.
Conduct a survey of oats and barley (n≥100) using mass spectrometry to identify contamination levels of, not only the mycotoxins of interest, i.e., T-2 and HT-2, but also all other regulated mycotoxins including aflatoxins, deoxynivalenol, zearalenone, fumonisins and ochratoxin A.
• Perform a thorough evaluation of a selected number of rapid test kits to ascertain fitness for purpose and determine how to adapt the most robust methods for better performance. If required, undertake a training workshop on the use of the best performing rapid testing kits.
• Engage with stakeholders to discuss the implications of the findings of
the project and produce a series of conclusions and recommendations
for stakeholders on the island of Ireland.

Research- Monitoring of Animal Feed Contamination by Mycotoxins: Results of Five Years of Official Control by an Accredited Italian Laboratory

MDPI

Abstract

Mycotoxin contamination of animal feed is a complex issue in both animal wellness and food safety. The most diffused mycotoxins subject to the official control of animal feed are Aflatoxin B1 (AF), Zearalenone (ZEA), Deoxynivalenol (DON), Ochratoxin A (OCRA), Fumonisins (FUMO), and T-2/HT-2 toxins. This work describes the results of five years of monitoring focused on the evaluation of mycotoxin contamination of animal feed. Analytical determinations were carried out by means of accredited ELISA. The obtained results showed a non-alarming scenario, with several samples resulting as “non-compliant” according to the Maximum Residue Limits (MRLs) set in European Regulation No. 574/2011. Out of 722 analyzed samples coming from 2 Italian regions, Apulia and Basilicata, 14 samples were characterized by mycotoxin concentrations higher than related MRL; in particular, 5, 4, and 5 non-compliant samples for DON, AF, and ZEA, respectively. This study also evaluated the possible correlations between mycotoxin type and feed use with a special focus on animal sensitivity to mycotoxins.

Research – The Investigation of Mycotoxins and Enterobacteriaceae of Cereal-Based Baby Foods Marketed in Turkey

MDPI

In this study, a total of 85 cereal-based baby foods with or without milk (four different brands; A, B, C, and D) collected from Ankara local markets, Turkey were analyzed for mycotoxins, total aerobic mesophilic bacteria (TAMB), and Enterobacteriaceae contamination. Baby foods were analyzed for 12 toxicological important mycotoxins such as aflatoxin B1, B2, G1, and G2; fumonisin B1 and B2; ochratoxin A; sterigmatocystin (STE); deoxynivalenol (DON); zearalenone (ZON); and T-2 toxin and HT-2 toxin by LC-MS/MS multi-mycotoxin method. In addition to these mycotoxins, the presence of aflatoxin M1 (AFM1) was investigated in baby foods containing milk. The classical culture method was used for microbiological analysis. Consequently, at least one mycotoxin was detected in 69.41% of the total samples. The most frequently detected mycotoxins were STE (34.12%) and HT-2 (34.12%). However, AFM1 was not detected in any of the baby foods containing milk. Also, TAMB and Enterobacteriaceae were isolated from 30.59% and 10.59% of samples, respectively. As a result, it was determined that the mycotoxin levels in the analyzed samples were in accordance with the mycotoxin levels specified in the Turkish Food Codex.

RASFF Alert – Mycotoxin T-2/HT-2 – Wheat Flour

RASFF-Logo

RASFF – T-2 toxin (106.7 µg/kg – ppb) and HT-2 toxin (346 µg/kg – ppb) in wheat flour from Belgium in Belgium

Abstract

T-2 toxin (T2) and HT-2 (HT2) toxin are trichothecenes, which form part of the group of Fusarium mycotoxins. Food and feed samples used to estimate human dietary and animal exposure were reported either as the individual results for T2 and/or, HT2, and/or as the sum of the two. The highest concentrations were reported in oats and oat-containing commodities. Very high levels were reported in a small number of data on specific plant- and herb-based dietary supplements. In humans, the mean chronic dietary exposure to the sum of T2 and HT2 was highest in‘Toddlers’and‘Infants’, with maximum upper bound (UB) estimates of 64.8 and 62.9 ng/kg body weight (bw) per day, respectively.The 95th percentile dietary exposure was highest in‘Infants’with a maximum UB estimate of 146 ng/kgbw per day. UB estimations were on average four fold higher than lower bound (LB) estimations. Average acute exposure ranged from a minimum of 13.4 ng/kg bw per day, estimated in‘Elderly’,up to a maximum of 64.7 ng/kg bw per day estimated in‘Toddlers’. The highest 95th percentile acute dietary exposure was estimated for a dietary survey within the age class‘Infants’(170 ng/kg bw per day).Overall, among processed foods the main contributors were cereal flakes,fine bakery wares and, for acute exposure, also bread and rolls. In the elderly and very elderly, dietary supplements made an important contribution. Exposure to the sum of T2 and HT2 in farm and companion animals varied according to the animal species. Exposures considering mean concentration scenarios varied between 0.03–0.08 (LB–UB)lg/kg bw per day in beef cattle and 1.13–1.47lg/kg bw per day in milking goats. Forhigh concentration scenarios, exposures varied between 0.12–0.16lg/kg bw per day and 2.37–2.58lg/kg bw per day in the same species. In the absence of data, potential modified form were not included.

©2017 European Food Safety Authority.EFSA Journal published by John Wiley and Sons Ltd on behalf of European Food Safety Authority