Category Archives: Bacillus

RASFF Alert- Bacillus cereus – Chilli Sauce

RASFF

Bacillus cereus in chilli sauce, from China in  Austria, Belgium, China, Finland, France, Germany, Hong Kong, Ireland, Malta, Netherlands, Romania, Sweden.

Hong Kong – Do not consume a batch of bottled preserved bean curd due to excessive level of Bacillus cereus

CFS

Issue Date 11.6.2024
Source of Information Food Surveillance Programme, Centre for Food Safety
Food Product Bottled preserved bean curd
Product Name and Description Product name: Preserved Beancurd

Brand: Yuet Wo

Place of origin: China

Net weight: 12 ounces

Best-before date: May 30, 2025

Distributor: Yuet Wo Sauce And Preserved Fruits Limited

Reason For Issuing Alert The CFS collected the above-mentioned sample from a retail outlet in Tsuen Wan for testing under its routine Food Surveillance Programme. The test result showed that the sample contained Bacillus cereus at a level of 130,000 per gram. According to the Microbiological Guidelines for Food, if ready-to-eat food contains Bacillus cereus at a level of more than 100,000 per gram, it is considered unsatisfactory.

Bacillus cereus is commonly found in the environment. Unhygienic conditions in food processing and storage may give rise to its growth. Consuming food contaminated with excessive Bacillus cereus or its heat-stable toxins may cause gastrointestinal upset such as vomiting and diarrhoea.

Action Taken by the Centre for Food Safety CFS has informed the vendor concerned of the irregularity and has instructed the vendor to stop sales and to remove from shelves the affected batch of the product. The distributor concerned has initiated a recall of the affected batch of the product according to the CFS’s instructions.

The CFS will alert the trade to the incident, and will continue to follow up and take appropriate action. An investigation is ongoing.

Advice to the Trade The trade should stop using or selling the product concerned immediately if they possess it.
Advice to Consumers Not to consume the affected product if they have bought any.
Further Information The CFS press release

Members of the public may call the hotline of the distributor at 2492 3354 during office hours for enquiries about the recall.

Centre for Food Safety

Belgium – Wangzhihe brand chili sauce – Bacillus cereus

AFSCA

chilisaus

chilisaus

The FASFC withdraws Wangzhihe brand chili sauce due to the possible presence of Bacillus cereus.

The FASFC asks not to use this product and to return it to the point of sale from which it was purchased.

Product Description :

– Product category: chili sauce

– Brand: Wangzhihe

– Barcode: 5013635101917

– Expiration date (DDM): 05/09/2025

– Weight: 240g

The product was distributed through various points of sale.

For any further information, you can contact the AFSCA contact point for consumers: 0800/13.550 or pointdecontact@afsca.be.

RASFF Alert- Bacillus cereus – Tempah

RASFF

Bacillus cereus in tempeh from Denmark in Germany and Sweden

Exposure assessment of Bacillus cereus introduced into reconstituted milk production in China

Science Direct

Abstract

Bacillus cereus (B. cereus) is one of the common food-borne pathogens in humans and has a high tolerance to the environment. Considering the high lethal temperature of B. cereus spores, it is difficult to be effectively killed by common pasteurization methods, resulting in a certain amount of residual spores in milk. In this paper, a quantitative risk assessment model of B. cereus was constructed to assess the risk of introducing B. cereus in the production of reconstituted milk based on the collected detection data of B. cereus contamination in Chinese milk powder. As a result, it is found that B. cereus is relatively seriously polluted on the pipeline from mixing tank to UHT sterilization (with a mean concentration of 5.17 log cfu) in the production process of reconstituted milk. This study provides a theoretical basis for formulating B. cereus contamination control measures in the production of reconstituted milk related products.

Research – Inactivation mechanism of phenyllactic acid against Bacillus cereus spores and its application in milk beverage

Science Direct

Phenyllactic acid (PLA) as a natural phenolic acid exhibits antibacterial activity against non-spore-forming bacteria, while the inhibitory effect against bacterial spore remained unknown. Herein, this study investigated the inactivation effect of PLA against Bacillus cereus spores. The results revealed that the minimum inhibitory concentration of PLA was 1.25 mg/mL. PLA inhibited the outgrowth of germinated spores into vegetative cells rather than germination of spores. PLA disrupted the spore coat, and damaged the permeability and integrity of inner membrane. Moreover, PLA disturbed the establishment of membrane potential due to the inhibition of oxidative metabolism. SEM observations further visualized the morphological changes and structural disruption caused by PLA. Besides, PLA caused the degradation of DNA of germinated spores. Finally, PLA was applied in milk beverage, and showed promising inhibitory effect against B. cereus spores. This finding could provide scientific basis for the application of PLA against spore-forming bacteria in food industry.

Hungary – Dragon Superfoods Organic In Shape Mix 200 g – Bacillus cereus and Mould/Mold

NEBIH

Dragon Superfoods Organic In Shape Mix 200 g

Date 2024-05-16
Name of Product Dragon Superfoods Organic In Shape Mix 200 g
Nature of the problem Presence of Bacillus cereus and molds
DetailsNébih asks customers not to consume the product that matches the identification data below!

Notifier: Culinaris Retail Kft.

Product name: Dragon Superfoods Organic In Shape Mix 200 g

Brand: Dragon Superfoods

Product origin: Bulgaria

Nature of the problem: presence of Bacillus cereus and molds

Shelf life: 02/04/2025

Item number: 02/04/2025

Packaging: 200 g

Manufacturer: Smart Organic Bulgaria

Distributor: Culinaris Retail Kft. (Budapest)

Action: Product recall, withdrawal from circulation

Culinaris Retail Kft. informed its customers about the recall on its website .

The affected product can be returned to any Culinaris store, the price of the product will be refunded even without a block. 

You can read more about the nature of the problem here .

The product recall only applies to the product identified by the above data.

Research – Microbial community structure of plant-based meat alternatives.

Nature

A reduction in animal-based diets has driven market demand for alternative meat products, currently raising a new generation of plant-based meat alternatives (PBMAs). It remains unclear whether these substitutes are a short-lived trend or become established in the long term. Over the last few years, the trend of increasing sales and diversifying product range has continued, but publication activities in this field are currently limited mainly to market research and food technology topics. As their popularity increases, questions emerge about the safety and nutritional risks of these novel products. Even though all the examined products must be heated before consumption, consumers lack experience with this type of product and thus further research into product safety, is desirable. To consider these issues, we examined 32 PBMAs from Austrian supermarkets. Based on 16S rRNA gene amplicon sequencing, the majority of the products were dominated by lactic acid bacteria (either Leuconostoc or Latilactobacillus), and generally had low alpha diversity. Pseudomonadota (like Pseudomonas and Shewanella) dominated the other part of the products. In addition to LABs, a high diversity of different Bacillus, but also some Enterobacteriaceae and potentially pathogenic species were isolated with the culturing approach. We assume that especially the dominance of heterofermentative LABs has high relevance for the product stability and quality with the potential to increase shelf life of the products. The number of isolated Enterobacteriaceae and potential pathogens were low, but they still demonstrated that these products are suitable for their presence.

Research – An In-Depth Study on the Inhibition of Quorum Sensing by Bacillus velezensis D-18: Its Significant Impact on Vibrio Biofilm Formation in Aquaculture

MDPI

Abstract

Amid growing concerns about antibiotic resistance, innovative strategies are imperative in addressing bacterial infections in aquaculture. Quorum quenching (QQ), the enzymatic inhibition of quorum sensing (QS), has emerged as a promising solution. This study delves into the QQ capabilities of the probiotic strain Bacillus velezensis D-18 and its products, particularly in Vibrio anguillarum 507 communication and biofilm formation. Chromobacterium violaceum MK was used as a biomarker in this study, and the results confirmed that B. velezensis D-18 effectively inhibits QS. Further exploration into the QQ mechanism revealed the presence of lactonase activity by B. velezensis D-18 that degraded both long- and short-chain acyl homoserine lactones (AHLs). PCR analysis demonstrated the presence of a homologous lactonase-producing gene, ytnP, in the genome of B. velezensis D-18. The study evaluated the impact of B. velezensis D-18 on V. anguillarum 507 growth and biofilm formation. The probiotic not only controls the biofilm formation of V. anguillarum but also significantly restrains pathogen growth. Therefore, B. velezensis D-18 demonstrates substantial potential for preventing V. anguillarum diseases in aquaculture through its QQ capacity. The ability to disrupt bacterial communication and control biofilm formation positions B. velezensis D-18 as a promising eco-friendly alternative to conventional antibiotics in managing bacterial diseases in aquaculture.

Research – Exploring Propolis as a Sustainable Bio-Preservative Agent to Control Foodborne Pathogens in Vacuum-Packed Cooked Ham

MDPI

Abstract

The search for natural food additives makes propolis an exciting alternative due to its known antimicrobial activity. This work aims to investigate propolis’ behaviour as a nitrite substitute ingredient in cooked ham (a ready-to-eat product) when confronted with pathogenic microorganisms of food interest. The microbial evolution of Listeria monocytogenesStaphylococcus aureusBacillus cereus, and Clostridium sporogenes inoculated at known doses was examined in different batches of cooked ham. The design of a challenge test according to their shelf life (45 days), pH values, and water activity allowed the determination of the mesophilic aerobic flora, psychotropic, and acid lactic bacteria viability. The test was completed with an organoleptic analysis of the samples, considering possible alterations in colour and texture. The cooked ham formulation containing propolis instead of nitrites limited the potential growth (δ < 0.5 log10) of all the inoculated microorganisms until day 45, except for L. monocytogenes, which in turn exhibited a bacteriostatic effect between day 7 and 30 of the storage time. The sensory analysis revealed the consumer’s acceptance of cooked ham batches including propolis as a natural additive. These findings suggest the functionality of propolis as a promising alternative to artificial preservatives for ensuring food safety and reducing the proliferation risk of foodborne pathogens in ready-to-eat products.