Category Archives: STX 2

Research -Occurrence of Campylobacter spp., Salmonella spp. and shiga toxin‐producing Escherichia coli in inline milk filters from Swedish dairy farms

Wiley Online

Abstract

This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from 12.6% of filters. The stx genes (stx 1stx 2, or both) were screened from 71% of the filters and STEC was isolated from 14% of these. Of the STEC isolates, 21 contained the stx 1 gene, 19 the stx 2 gene, and five a combination of both stx 1 and stx 2 genes. Whole genome sequence typing on 34 of the 45 STEC showed that they belonged to 21 different serotypes, of which STEC O145:H28 was the most common (2%). STEC O157:H7 was only found from one (0.3%) of the filters. A combination of stx 2 and eae genes was found from 0.7% of the total number of inline milk filters analyzed, while stx 2a was found in 24% of the whole genome‐sequenced isolates. There was a significant positive correlations between number of animals per farm and presence of pathogens on milk filters.

Research – Survey of Intact and Nonintact Raw Pork Collected at Retail Stores in the Mid-Atlantic Region of the United States for the Seven Regulated Serogroups of Shiga Toxin–Producing Escherichia coli

Journal of Food Protection

ABSTRACT

A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga toxin–producing Escherichia coli (STEC-7) using standard microbiological and molecular methods. In short, 21 (5.3%) of the 395 ground or nonintact pork samples and 3 (3.4%) of the 119 intact pork samples tested positive via the BAX system real-time PCR assay for the stx and eae virulence genes and for the somatic O antigens for at least one of the STEC-7 serogroups. However, none of these 24 presumptive-positive pork samples subsequently yielded a viable isolate of STEC displaying a STEC-7 serogroup-specific surface antigen in combination with the stx and eae genes. These data suggest that cells of STEC serogroups O26, O45, O103, O111, O121, O145, or O157:H7 are not common in retail raw pork samples in the mid-Atlantic region of the United States.

HIGHLIGHTS
  • None of the 514 retail raw pork samples were positive for STEC-7.

  • Four of 514 raw pork samples harbored E. coli of unknown serogroup containing stx and eae.

  • STEC-7 are uncommon in retail raw pork samples in the U.S. mid-Atlantic region.

 

RASFF Alert – STEC E.coli – Frozen VP Buffalo Sausages

RASFF-Logo

RASFF – shigatoxin-producing Escherichia coli (stx1+ stx2+ /25g) in frozen vacuum-packed buffalo sausages from Belgium in the Netherlands

RASFF Alerts – STEC E.coli – Lamb Carcase – Bovine Meat

RASFF-Logo

RASFF -enteropathogenic Escherichia coli (O157 stx1+, stx2-, eae+) in lamb carcass from Belgium in Belgium

RASFF -shigatoxin-producing Escherichia coli (O103 stx1+ stx2- eae+ /25g) in frozen bovine meat from Uruguay in Finland

RASFF Alerts – STEC E.coli – Frozen Beef

Last two weeks catch up

RASFF-Logo

RASFF – shigatoxin-producing Escherichia coli (stx1+ /25g) in frozen boneless beef topside from Brazil in the Netherlands

RASFF – shigatoxin-producing Escherichia coli ( stx2+ eae-; stx2+ eae+ present /25g) in frozen beef from Uruguay in Finland

RASFF Alert -STEC E.coli – Chilled Beef

RASFF-Logo

RASFF – shigatoxin-producing Escherichia coli (stx1+, stx2+,) in chilled beef from Argentina in Italy

Summary of the last two weeks RASFF Alerts – STEC E.coli – Frozen Minced Meat – Chilled Beef

RASFF-Logo

RASFF – shigatoxin-producing Escherichia coli (O157:H7, O26:H11) in frozen beef minced meat with raw material from Spain in France

RASFF – shigatoxin-producing Escherichia coli (stx2+) in chilled beef from Argentina in Italy

Research – Whole Genome Sequencing Characterization of Shiga Toxin–Producing Escherichia coli Isolated from Flour from Swiss Retail Markets

Journal of Food Protection

ABSTRACT

Shiga toxin–producing Escherichia coli (STEC) strains are often found in food and cause human infections. Although STEC O157:H7 is most often responsible for human disease, various non-O157 subtypes have caused individual human infections or outbreaks. The importance of STEC serogroup typing is decreasing while detection of virulence gene patterns has become more relevant. Whole genome sequencing (WGS) reveals the entire spectrum of pathogen information, such as toxin variant, serotype, sequence type, and virulence factors. Flour has not been considered as a vector for STEC; however, this product has been associated with several STEC outbreaks in the last decade. Flour is a natural product, and milling does not include a germ-reducing step. Flour is rarely eaten raw, but the risks associated with the consumption of unbaked dough are probably underestimated. The aim of this study was to determine the prevalence of STEC in flour samples (n = 93) collected from Swiss markets and to fully characterize the isolates by PCR assay and WGS. The prevalence of STEC in these flour samples was 10.8% as indicated by PCR, and a total of 10 STEC strains were isolated (two flour samples were positive for two STEC subtypes). We found one stx2-positve STEC isolate belonging to the classic serogroups frequently associated with outbreaks that could potentially cause severe disease. However, we also found several other common or less common STEC subtypes with diverse virulence patterns. Our results reveal the benefits of WGS as a characterization tool and that flour is a potentially and probably underestimated source for STEC infections in humans.

HIGHLIGHTS
  • Several STEC serotypes, including O26, were isolated from 8 (8.6%) of 93 flour samples.

  • STEC isolates from flour had a variety of virulence patterns.

  • Flour is a probably underestimated source of STEC infections in humans.

  • WGS for STEC characterization is more comprehensive than common serotyping.

RASFF Alerts – STEC E.coli – Soft Cheese

RASFF-Logo

RASFF – shigatoxin-producing Escherichia coli (stx1+ stx2- eae-) in soft cheese from Belgium in Germany

UK – England: Health officials warn of E. coli STEC infections in individuals returning from Egypt

Outbreak News Today

British health officials are advising travelers to Egypt of a number of Shiga toxin-producing Escherichia coli (STEC) infections reported in people who traveled to the Hurghada region of Egypt.

There have been 18 cases of STEC in individuals returning from Egypt in 2019, including one case of Hemolytic Uremic Syndrome (HUS).

E. coli can cause an unpleasant diarrhea illness with stomach cramps and occasionally fever. Most people will recover without the need for medical treatment, but younger and older people may go on to develop complications of the infection, leading to kidney failure. This rare condition is called hemolytic uremic syndrome (HUS), which in very rare circumstances can be fatal.

E. coli is caught through ingesting contaminated food or water.

PHE

PHE recommends travellers to the region to:

  • where possible, avoid eating salads and uncooked vegetables
  • only eat fruit they can peel
  • avoid unpasteurised milk, cheese and ice cream
  • avoid food that has been left uncovered in warm environments and exposed to flies
  • ensure all meat is cooked thoroughly before you eat it, avoiding any meat that is pink or cold
  • avoid ice, unless made with filtered or bottled water, and tap water, even when brushing teeth
  • only drink bottled water or use ice made from bottled/filtered water
  • wash your hands thoroughly after visiting the toilet, and always before preparing or eating food. Alcohol gel can be helpful (but not entirely effective) when hand washing facilities are not available
  • when swimming, try and avoid swallowing water where possible and supervise children when swimming.
  • don’t swim whilst ill

For more information, visit NHS.UK.

This advice also applies to other countries where E. coli infections are common, including Turkey and Spain.

Dr Nick Phin, Deputy Director, National Infection Service, Public Health England, said:

We are aware of people returning from Egypt with E. coli infections, some with a serious kidney complication called haemolytic uraemic syndrome (HUS). We are gathering information about those affected to better understand the cause.

There are simple precautions that travellers can take. These include ensuring meat is cooked thoroughly, not drinking tap water or ice made from tap water and trying to avoid swallowing water when swimming.

Anyone suffering from diarrhoea and vomiting should ensure they keep well hydrated and seek medical advice if their symptoms don’t improve within 48 hours. They should also avoid preparing or serving food while they have symptoms and thoroughly wash their hands after using the toilet to stop the bug being passed to others. Individuals with symptoms after returning from holiday should seek medical advice from their GP or NHS 111.