Category Archives: Pathogen

Research – Researchers identify biofilm hotspots, document danger zones

Food Safety News

Scientists in Austria have investigated where biofilms hide and what bacteria can be found in them.

Biofilms are proven sources of contamination in the food industry. They can cause additional costs in production and can be a danger to consumer health.

Researchers at the unit of food microbiology at Vetmeduni Vienna looked at biofilms in an Austrian meat processing environment that included pork, poultry and beef. Knowledge gained on presence and composition, published in the International Journal of Food Microbiology, could help to prevent and reduce biofilm formation within food processing environments.

USA -Fresh Express’s cyclospora outbreak now spans eight states and three provinces

Food Safety News

A troublesome Cyclospora outbreak continues to grow in North America with 37 confirmed cases in Canada added to 206 in the midwest United States. Cyclospora is a coccidian parasite that causes diarrheal disease in humans called cyclosporiasis.

The update today involves an outbreak of Cyclospora infections traced to Fresh Express bagged garden salad products containing lettuce, carrots, and red cabbage.

As of July 8 there are 37 confirmed cases of Cyclospora illness linked to this outbreak in three Canadian provinces: Ontario (26), Quebec (10) and Newfoundland and Labrador (1).  Canadians became sick between mid-May and mid-June.  One Canadian has been hospitalized. No deaths have been reported. Individuals who became ill are between 21 and 70 years of age. The majority of cases, 76 percent, are female.

Research – Risk of Foodborne Illness from Pet Food: Assessing Pet Owners’ Knowledge, Behavior, and Risk Perception

JFP

Pet food has been identified as a source of pathogenic bacteria, including Salmonella and Escherichia coli. A recent outbreak linked to Salmonella -contaminated pet treats infected over 150 people in the United States. The mechanism by which contaminated pet food leads to human illness has not been explicated. Pet owners’ food safety knowledge and their pet food handling practices have not been reported. This study evaluated pet owners’ food safety knowledge and pet-food handling practices through an online consumer survey. The survey consists of 62 questions and assesses (1) owners’ food safety knowledge and pet-food handling practices; (2) owners’ interaction with pets; (3) owners’ risk perception related to their own health, their children’s health, and their pets’ health. The survey was pilot-tested among 59 pet owners before distribution to a national consumer panel, managed by Qualtrics XM. All participants (n=1,040) were dog and/or cat owners in the United States. Almost all pet owners interacted with their pets (93%) and most cuddled, allowed their pets to lick them, and slept with their pets. Less than one-third of pet owners washed their hands with soap after interacting with their pets. Over half (58%) the owners reported washing their hands after feeding their pets. Most pet owners fed their pets dry pet food and dry pet treats. Some fed their pets raw meat or raw animal product (RAP) diets because they believed these diets to be beneficial to their pet’s overall health. Many owners (78%) were unaware of pet food recalls or outbreaks associated with foodborne pathogens. Less than 25% considered dry pet foods and treats as a potential source of foodborne pathogens. The findings of this study indicated the need for consumer education about pet food handling. The data collected can assist in developing more accurate risk assessment models and consumer education related to pet food handling.

Research -Cyclospora: A Long-term Challenge for Food Suppliers

World Aware

Outbreaks of cyclosporiasis, an illness caused by the parasite Cyclospora cayetanensis, occur almost every year in the US, and this summer is no exception. People can become infected with Cyclospora by consuming food or water contaminated with the parasite. Since May 1, more than 206 laboratory-confirmed cases of cyclosporiasis have been reported across 8 states in the Midwest.

Based on interviews with patients, investigators traced the outbreak back to bagged salad mix. The recent outbreak of Cyclospora infections highlights the importance of compliance with the Food and Drug Administration Produce Safety Rule and specifically worker health and hygiene principles.

The Centers for Disease Control and Prevention (CDC) and the Food and Drug Administration (FDA) have made attempts to better understand the factors contributing to Cyclospora infections.  However, many cases of cyclosporiasis cannot be directly linked to an outbreak, in part because of the lack of validated molecular typing tools for Cyclospora cayetanensis.

It is likely, in the future, new analysis methods will be developed to differential strains of Cyclospora, if there is enough genetic diversity. This will allow a way to focus more quickly on illness clusters and more rapid traceback of food vehicles to production sites. This should allow for environmental assessments at production sites to determine routes of contamination and prevention option.

In the meantime, the best we can do is to emphasize the importance of compliance with the Food and Drug Administration Produce Safety Rule, and specifically worker health and hygiene principles.

 

 

Research -Sneaky Salmonella finds a backdoor into plants

News Wise

Newswise — As the world wrestles with the coronavirus (COVID-19) pandemic, which arose after the virus jumped from an animal species to the human species, University of Delaware researchers are learning about new ways other pathogens are jumping from plants to people.

Opportunistic bacteria — salmonella, listeria and E.coli, for example — often piggyback on raw vegetables, poultry, beef and other foods to gain entry into a human host, causing millions of foodborne illnesses each year.

But University of Delaware researchers Harsh Bais and Kali Kniel and their collaborators now have found that wild strains of salmonella can circumvent a plant’s immune defense system, getting into the leaves of lettuce by opening up the plant’s tiny breathing pores called stomates.

The plant shows no symptoms of this invasion and once inside the plant, the pathogens cannot just be washed off.

Stomates are little kidney-shaped openings on leaves that open and close naturally and are regulated by circadian rhythm. They open to allow the plant to cool off and breathe. They close when they detect threats from drought or plant bacterial pathogens.

Research – A bacterium that causes food-borne illness grows flagella under stressful conditions

Massive Science CDC E.coli

Micro-organisms, especially bacteria, play essential roles in our bodies, especially in our guts. Some bacteria are beneficial, and some like E.coli are harmful. Another Escherichia strain (in the same genus as E. coli) named Escherichia albertii is also pathogenic to humans, causing diarrhea and food-borne illnesses.  E. albertii was identified for the first time during an illness outbreak in Bangladesh.

Pathogenic bacteria like E. albertii are very motile, meaning they move around a lot. They are able to do this using hair-like structures called flagellaE. albertii was originally described as non-hairy bacterium and thus far has been considered to be a non-motile pathogenic micro-organism.

 

Research -The European Union control strategy for Campylobacter spp. in the broiler meat chain

Wiley Online

Campylobacter is the most common cause of bacterial gastroenteritis worldwide and the most frequently reported foodborne pathogen in the European Union (EU). While campylobacteriosis is generally self‐limiting, some patients could develop severe sequelae. The predominant source of infection is poultry. This review addresses the most relevant factors influencing the prevalence and contamination level of Campylobacter spp. in the poultry chain continuum. The emphasis was put on the novel control strategy for Campylobacter that is based on evidence‐based risk assessment and the introduction of process hygiene criterion intended for monitoring the prevalence and counts of Campylobacter spp. on broiler carcasses at slaughter level. The reduction of Campylobacter spp. in the poultry meat chain in the EU can only be achieved with an integrated meat safety assurance approach. This includes primary interventions at the level of the poultry farm, implementation of effective control measures at slaughterhouses, and fostering awareness campaigns aimed at consumers.

 

Research – Examination of the Culturable Microbiota from Low-Moisture Foods Imported into Canada for Antibacterial Activity against Listeria monocytogenes

Journal of Food Protection

ABSTRACT

Listeria monocytogenes, a resilient and ubiquitous foodborne pathogen, is associated with a high case-fatality rate in humans. This study investigated the culturable microbiota of low-moisture foods (LMFs) imported into Canada to see how well bacteria isolated from these foods could inhibit or inactivate the growth of L. monocytogenes. Imported LMFs were acquired from various supermarkets in the Greater Toronto Area (Ontario, Canada). The foods included dried apples, bee pollen, cumin seeds, date fruits, fennel seeds, pistachios, raisins, and seaweed. Bacterial strains were isolated from the foods using blood agar and then screened using an in-house-designed growth inhibition plate assay against L. monocytogenes. The inhibitory strains detected were then identified using 16S rRNA sequencing. Diverse bacteria were recovered from the foods; 236 isolates belonging to 122 observed phenotypes were obtained. From the inhibition plate assays, 10 of the 11 imported LMFs harbored inhibitory strains against L. monocytogenes, whereby 48 of the collected isolates (20%) were found to produce a zone of inhibition against this pathogen. The inhibitory strains belonged to six genera (Acinetobacter, Aerococcus, Bacillus, Lysinibacillus, Paenibacillus, and Sporosarcina) and 15 unique species. Among all foods tested, the date fruit microbiota displayed the greatest number and diversity of anti–L. monocytogenes inhibitory strains. Overall, it was found that the culturable microbiota of LMFs, imported into Canada, possess bacterial members that can inhibit the growth of L. monocytogenes. These results could lead to the discovery of either novel antimicrobial metabolites or beneficial anti–L. monocytogenes bacteria that could be added to foods to inactivate and/or control L. monocytogenes.

HIGHLIGHTS
  • Modification to an inhibition plate assay detects anti-Listeria bacteria more rapidly.
  • Unconventional anti-Listeria genera, such as Lysinibacillus, were discovered.
  • Date fruit microbiota warrants further investigation for anti-Listeria applications.
  • Isolated bacteria from low-moisture foods are diverse and can inhibit L. monocytogenes growth.

Research – Pet ownership and pet type and their implications for food safety in the home: Evidence from a national survey

Journal of Food Protection

Physical contact between humans and their pets increases the potential for zoonotic disease transmission. This study used the 2016 FDA Food Safety Survey to compare the food handling behaviors of pet owners and non-pet owners, since poor food handling and hygiene habits can increase the likelihood of disease transmission from animals to humans. Results show that both pet ownership and pet type were important in predicting food safety behaviors. After controlling for sociodemographic factors included in this study (gender, age, household income, household size, and race/ethnicity), pet ownership was significantly associated with overall food safety practices, and more specifically with better handwashing behaviors, kitchen cleaning, and ownership and use of a food thermometer, as well as a greater awareness of foodborne pathogens. Cat owners and cat/dog owners had better overall food safety practices and better handwashing compared to dog owners. After controlling for sociodemographic variables, there were no significant associations between pet ownership and perception of risks associated with unsafe food handling practices.

Research – Study finds olive leaf extract can inhibit the growth of foodborne pathogens

New Food Magazine

The extract, from Euromed, was found to inhibit the growth of Listeria monocytogenes and could be a potential adjunct to help control foodborne pathogens.

A recently published study has found that olive leaf extract from Euromed, standardised herbal extract and active pharmaceutical ingredients producer, inhibits the growth of Listeria monocytogenes bacteria.